Radicle emergence test as a quick vigour test to predict field emergence performance in rice (Oryza sativa L.) seed lots
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Abstract
An experiment was made to standardize the radicle emergence test to predict the field emergence performance in ten different seed lots [L1 to L4: high vigour lots (> 90 % germination), L5 to L7: medium vigour lots (80-90 % germination) and L8 to L10: low vigour lots (< 80 % germination)] of rice cv. CO 51. The results showed that the significant differences are observed in physiological and biochemical parameters in different seed lots. The seed vigour was classified into three groups viz., high, medium and low vigour based on the relationship between mean germination time and field emergence. When the Mean Germination Time (MGT) was < 34 hours, the field emergence was > 85 per cent, which was considered as high vigour; when the MGT was 34-35 hours, the field emergence was 80-85 per cent, that was considered as medium vigour; when the MGT was > 35 hours, the field emergence was < 80 per cent, that was considered as low vigour. The radicle emergence test (2mm radicle length) was highly negatively correlated with mean germination time (-0.930**) followed by mean just germination time (-0.852**) and electrical conductivity of seed leachate (-0.827**) and it was positively correlated with field emergence (0.894**) followed by germination (0.878**) and dehydrogenase activity (0.864**). The R2 values between seed vigour parameters and radicle emergence test were significantly higher in 2mm length of radicle emergence when compared with 1mm length of radicle emergence. Finally, the study concluded that 36 hour MGT with the attainment of 2mm radicle emergence percentage could be used as a quick method to assess rice seed lots' quality by the seed analysts and seed industry.
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Article Details
Field emergence, Germination, Radicle emergence, Rice, Vigour
Demir, I., Ermis, S., Mavi, K. & Matthews, S. (2008). Mean germination time of pepper seed lots (Capsicum annuum L.) predicts size and uniformity of seedlings in germination tests and transplant modules. Seed Science and Technology, 36(1), 21-30. DOI: 10.15258/sst.2010.38.1.02.
Ellis, R. H. & Roberts, E. H. (1980). The influence of temperature and moisture on seed viability period in barley (Hordeum distichum L.). Annals of Botany, 45(1), 31-37.
International Seed Testing Association (2012). International Rules for Seed Testing. International Seed Testing Association (ISTA). Bassersdorf, Switzerland.
International Seed Testing Association (2014). Seed Vigour Testing. International Rules for Seed Testing, , International Seed Testing Association (ISTA). Zurich, Switzerland.
International Seed Testing Association. (2016). Seed Vigour Testing. International Rules for Seed Testing, , International Seed Testing Association (ISTA). Zurich, Switzerland.
Kittock, D. L. & Law, A. G. (1968). Relationship of seedling vigor to respiration and tetrazolium chloride reduction by germinating wheat seeds 1. Agronomy Journal, 60(3), 286-288.
Kolasinska, K., Szyrmer, J. & Dul, S. (2000). Relationship between laboratory seed quality tests and field emergence of common bean seed. Seed Science and Technology, 31(1), 54-70. (DOI: https://doi.org/10.2135/cropsci20 00.402 470x).
Larsen, S. Ugilt, Povlsen, F. Vestergaard, Eriksen, E. Nymann & Pedersen, H.C. (1998). "The influence of seed vigour on field performance and the evaluation of the applicability of the controlled deterioration vigour test in oil seed rape (Brassica napus) and pea (Pisum sativum). Seed Science and Technology, 26 (4), 627-641.
Luo, Y., Y.J. Guan, Y.T. Huang, J. Li, Z. Li. & J. Hu. (2015). Single counts of radicle emergence provides an alternative method to test seed vigour in sweet corn. Seed Science and Technology, 43 (3),519-525.
Maguire, J. D. (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor 1. Crop Science, 2(2), 176-177.
Matthews, S. & Powell, A. (2011). Towards automated single counts of radicle emergence to predict seed and seedling vigour. Seed Testing International (ISTA Bulletin), 142, 44-48.
Matthews, S., Noli, E., Demir, I., Khajeh-Hosseini, M. & Wagner, M. H. (2012). Evaluation of seed quality: from physiology to international standardization. Seed Science Research, 22(S1), S69-S73.
Mavi, K., Powell, A. A. & Matthews, S. (2016). Rate of radicle emergence and leakage of electrolytes provide quick predictions of percentage normal seedlings in standard germination tests of radish (Raphanus sativus). Seed Science and Technology, 44(2), 393-409. (DOI: https://doi.org/10.15258/sst.2016.44.2.12)
Navratil, R. J. & Burris, J. S. (1980). Predictive equations for maize inbred emergence 1. Crop Science, 20(5), 567-571. https://doi.org/10.2135/cropsci1980.001 1183X00200 0050004x
Naylor. (2003). Germination of seed lots of Italian ryegrass (Lolium multiflorum Lam.) after extended natural ageing in cool storage. Seed Science and Technology 31 (1), 177-185.
Powell. (1988). Seed vigour and field establishment. Advances in research and technology of seeds. 30(3), 56-66. (DOI: https://doi.org/10.1093/jxb/erv490).
Presley, J. T. (1958). Relation of protoplast permeability to cotton seed viability and predisposition to seedling disease. Plant Disease Reporter, 42(7), 852.
Qun, S., Wang, J. H. & Sun, B. Q. (2007). Advances on seed vigor physiological and genetic mechanisms. Agricultural Sciences in China, 6(9), 1060-10 66.
Suganthi, A. & Selvaraju, P. (2017). Fixing accelerated ageing test period and evaluation of physical, physiological and biochemical changes during accelerated ageing in groundnut CO 7 pods. International Journal of chemical studies, 5(6), 174-179.
Tekrony, D. M. & Egli, D. B. (1991). Relationship of seed vigor to crop yield: a review. Crop Science, 31(3), 816-822.
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