Neena Kumari Suresh Kumar


The Stevia genus encompasses about 200 herbs and shrubs species. Stevia rebaudiana, one of the members has gained commercial importance as a natural low calorie sweetener, due to the presence of high con-centration of stevioside and rebaudioside - A (25% to 45% of stevioside content) in the leaves. The major processes involved in the production and quantification of steviol glycosides are extraction, purification and estimation. Various extraction methods have been used for extraction of steviol glycosides in the world. The extraction methods of steviol glycosides mostly differed at the stage of clarification of extracts. The present study is an attempt to summarize the scattered literature and reports on a single podium. Moreover, it also depicts up to date literature regarding numerous extraction, purification and quantitative estimation methods for steviol glycosides


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Extraction, Purification, Quantitative Analysis, Rebaudioside-A, Stevia, Stevioside

Abdullateef, R.A. and Osman, M. (2012). Studies on effects of pruning on vegetative traits in Stevia rebaudiana Bertoni (compositae). Int. J. Biol., 4:146-153.
Abelyan, V.A., Balayan, A.M., Ghochikyan, V.T. and Markosyan, A.A. (2004). Transglycosylation of stevioside by cyclodextrin glucanotransferases of various groups of microorganisms. Appl. Biochem. Micro., 40:129-134.
Abelyan, V., Markosyan, A. and Abelyan, L. (2010). Process for manufacturing a sweetener and use thereof. US patent 8,298,601 B2.
Abou, A.E., Abou, A.A. and Abu, M.F. (2010). Physico-chemical assessment of natural sweeteners stevioside produced from Steviare baudiana Bertoni plant. Afr. J. Food Sci.,4: 269–281.
Afandi, A., Sarijan, S. and Shaha, R.K. (2013). Optimization of rebaudioside A extraction from Stevia rebaudiana (Bertoni) and quantification by high performance liquid chromatography analysis. J. Trop. Resour. Sustain. Sci., 1:62-70.
Ahmed, M.J. and Smith, R.M. (2002). Determination of stevioside by high performance liquid chromatography with pulsed amperometric detection. J. Sep. Sci.,25: 170−172.
Ahmed, M.S. and Dobberstein, R.H. (1982). Stevia rebaudiana II. High performance liquid chromatographic separation and quantification of stevioside, rebaudioside A and rebaudioside C. J. Chromatogr., 236: 523-526.
Ahmed, M.S., Dobberstein, R.H. and Farnsworth, N.R. (1980). Stevia rebaudiana I. Use of p-bromophenacyl bromide to enhance ultraviolet detection of water soluble organic acids (steviolbioside and rebaudioside B) in high performance liquid chromatographic analysis. J. Chromatogr., 192: 387-393.
Akashi, H., Yokoyama, Y. and Osada, M. (1975). Extraction and purification of steviosides. Japan Kokai 75-24300.
Anton, S., Martin, C., Han, H., Coulon, S., Cefalu, W. and Geiselman, P. (2010). Effects of stevia, aspartame, and sucrose on food intake, satiety and postprandial glucose and insulin levels. Appetite, 55: 37–43.
Anvari, M. and Khayat, G. (2016). Separation and purification of rebaudioside A from extract of Stevia rebaudiana leaves by macroporous adsorption resins. Pol. J. Chem. Technol., 18: 127–132.
Arya, A., Kumar, S. and Kasana, M.S. (2012). Anti-inflammatory activity of in vitro regenerated calli and in vivo plant of Stevia rebaudiana (Bert.) Bertoni. IJSRP., 2: 1-5.
Ayyappa, B., Kanchi, S., Singh, P., Myalowenkosi, I.S. and Bisetty, M.D.K. (2015). Analytical evaluation of steviol glycosides by capillary electrophoresis supported with molecular docking studies. J. Iran. Chem. Soc., 12: 127–136.
Benford, D.J., Di Novi, M. and Schlatter, J. (2006). Safety evaluation of certain food additives: steviol glycosides (PDF). WHO Food Additives Series (World Health Organization Joint FAO/WHO Expert Committee on Food Additives (JECFA), 54:140.
Blumenthal, M. (1996). Perspectives of FDA’S new stevia policy, after four years, the agency lifts its ban –but only partially, Whole Foods Magazine, Febraury 1–5.
Brandle, J. (1999). Genetic control of rebaudioside A and C in leaves of the sweet herb, Stevia rebaudiana. Can. J. Plant. Sci., 79: 85-92.
Cacciola, F., Delmonte, P., Jaworska, K., Dugo, P., Mondello, L. and Rader, J.I. (2011). Employing ultra high pressure liquid chromatography as the second dimension in a comprehensive two-dimensional system for analysis of Stevia rebaudiana extracts. J. Chromatogr. A, 1218: 2012–2018.
Carakostas, M., Curry, L., Boileau and Brusick, D. (2008). Overview: the history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverages. Food. Chem. Toxicol., 46: S1–S10
Carakostas, M., Prakash, I., Kinghorn, A.D., Wu, C.D. and Soejarto, D.D. (2012). Steviol glycosides. Alternative Sweeteners, 4th ed., Revised and Expanded.(ed O’Brien N.L.). Marcel Dekker: New York, NY, USA. pp. 159–180.
Chan, P., Xu, D.Y., Liu, J.C., Chen, Y.J., Tomlinson, B., Huang, W.P. and Cheng, J.T. (1998). The effect of stevioside on blood pressure and plasma catecholamines in spontaneously hypertensive rats. Life Sci.,63: 1679-1684.
Chang, S.S. and Cook, J.M. (1983). Stability studies of stevioside and rebaudioside A in carbonated beverages. J. Agr. Food. Chem.,31: 409-412.
Chatsudthipong,V. and Muanprasat, C. (2009). Stevioside and related compounds: therapeutic benefits beyond sweetness. Pharmacol.Therapeut.,121: 41-54.
Chaturvedula, V.S.P. and Zamora, J. (2014). Reversed-phase HPLC analysis of steviol glycosides isolated from
Stevia rebaudiana Bertoni. Food Nutr. Sci., 5: 1711-1716.
Chaturvedula, V.S.P., Chen, S., Yu, O. and Mao, G. (2013a). Isolation, NMR spectral analysis and hydrolysis studies of a hepta pyranosyl diterpene glycoside from Stevia rebaudiana Bertoni. Biomolecules, 3: 733–740.
Chaturvedula, V.S.P., Clos, J.F., Joshua, R., Milanowski, D., Mocek, U., Dubois, G.E. and Prakash, I. (2011). Minor diterpenoid glycosides from the leaves of Stevia rebaudiana. Phytochem.Lett., 4: 209–212.
Chaturvedula, V.S.P., Yu, S.C.O. and Mao, G. (2013b). NMR spectral analysis and hydrolysis studies of rebaudioside N, a minor steviol glycoside of Stevia rebaudiana Bertoni. Food Nutr. Sci., 4: 1004-1008.
Chaturvedula, V.S.P. (2014). Isolation and structural characterization of a new minor penta β-d-glucopyranosyl diterpene from Stevia rebaudiana Bertoni. Am. J. Plant Sci.,5: 3519-3525.
Chaya, S.C., Mondal. S., Majumdar, G.C. and De, S. (2011). Clarification of Stevia extract by ultrafiltration:
selection criteria of the membrane and effects of operating conditions. Food Bioprod. Process., 90: 525–532.
Cheng, T.F. and Chang, W.H. (1983). Studies on the non-stevioside components of stevia extracts. Ko Hsueh Fa Chan Yueh Kan, 11: 96-108.
Cheng, T.F., Chang, C.Y. and Chang, W.H. (1985). A study on the purification of steviosides with use of ion-exchange resins. J. Chinese Agri. Chem. Society, 23: 178-190.
Chester, K., Tamboli, E.T., Singh, M. and Ahmad, S. (2012). Simultaneous quantification of stevioside and
rebaudioside A in different stevia samples collected from the Indian subcontinent. J. Pharm. Bioall. Sci., 4: 276-81.
Choi, Y. H., Kim, I. and Yoon, K.D. (2002). Supercritical fluid extraction and liquid chromatographic-electrospray mass spectrometric analysis of stevioside from Stevia rebaudiana leaves. Chromatogr. A,55: 617−620.
Cioni, P.L., Morelli, L., Andolfi, L., Macchia, M. and Ceccarini, L. (2006). Qualitative and quantitative analysis of essential oils of five lines of Stevia rebaudiana Bert.genotypes cultivated in Pista (Italy). J. Essent. Oil Res., 18: 76-79.
Crammer, B. and Ikan, R. (1986). Sweet glycosides from the Stevia plants. Chem. Britain, 22: 915-916.
Dacome, A.S., Silva, C.C., Costa, C.E.M., Fontana, J.D., Adelmann, J. and Costa, S.C. (2005). Sweet diterpene glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods. J. Process Biochem., 40: 3587-3594.
Darise, M., Kohda, H., Mizutani, K., Kasai, R. and Tanaka, O. (1983). Chemical constituents of flowers of Stevia rebaudiana Bertoni. Agric. Biol. Chem., 47: 133-135.
Das, S., Das, A.K., Murphy, R.A., Punwani, I.C., Nasution, M.P. and Kinghorn, A.D. (1992). Evaluation of the cariogenic potential of the intense natural sweeteners stevioside and rebaudioside A. Caries Res., 26: 363–366.
Debnath, M. (2008). Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia
rebaudiana. J. Med. Plants Res., 2:45-51.
Deshmukh, S.R. and Kedari, V.R. (2014). Isolation, purification and characterization of sweetners from Stevia rebaudiana (Bertoni) for their anticancerous activity against colon cancer. World J. Pharm. Sci., 3: 1394-1410.
Englert, M., Kaiser, C., Schwack, W. and Vetter, W. (2016). Isolation of (five) steviol glycosides from a Stevia rebaudiana formulation by gradient elution countercurrent chromatography. Chromatogr. A, 79: 275–284.
Erkucuk, A., Akgun, I.H. and Celiktas, Y.O. (2009).
Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: identification and optimization. J. Supercrit. Fluid.,51: 29−35.
Fu, Q.U., Guo, Z., Zhang, X., Liu, Y. and Liang, X. (2012).Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography. J. Sep. Sci.,35: 1821–1827.
Fuh, W.S. and Chiang, B.H. (1990). Purification of steviosides by membrane and exchange processes. J. Food Chem., 55: 1454-1457.
Fujinuma, K., Saito, K., Nakazato, M., Kikuchi, Y., Ibe, A. and Nishima, T. (1986).Thin layer chromatographic detection and liquid chromatographic determination of stevioside and rebaudioside A in beverages and foods following reverse phase column chromatography. J. Assoc. Off. Anal. Chem.,69: 799-802.
Gardana, C., Scaglianti, M. and Simonetti, P. (2010). Evaluation of steviol and its glycosides in Stevia rebaudiana leaves and commercial sweetener by ultra-high performance liquid chromatography–mass spectroscopy. J.Chromatogr. A, 1217: 1463–1470.
Gasmalla, M.A.A., Yang, R. and Hua, X. (2014). Stevia rebaudiana Bertoni: an alternative sugar replacer and its application in food industry. Food Eng. Rev., 6: 150–162.
Gentry, A.H. (1996). Field guide of the families and genera of woody plants to Northwest South America with
supplementary notes on herbaceous Taxa. The University of Chicago Press, 895p.
Geuns, J.M.C. (2003). Stevioside. Phytochemistry, 64: 913–921.
Ghanta, S.A., Banerjee, A., Podder and Chattopadhyay, S. (2007).Oxidative DNA damage preventive activity and anti oxidant potential of Stevia rebaudiana (Bertoni) a natural sweetener.J. Agr. Food. Chem., 55: 962-967.
Giavanetto, R. H. (1990). Method for the recovery of steviosides from plant raw material. US patent 4, 892- 938.
Giuffre, L., Romaniuk, R. and Ciarlo, E. (2013). Stevia, ka'a he'e, wild sweet herb from South America - an overview.Emir. J. Food Agric., 25: 746-750.
Gonzalez, I.A., Yolanda, M.O. and David, B.A. (2014). Validation of HPLC-UV method for determination of minor glycosides contained in Stevia rebaudiana Bertoni leaves. Biomed. Chromatogr., 29:733-738.
Gonzalez, I.A., Ordonez, Y.M. and Ancona, D.B. (2015). Determination of rebaudioside A and stevioside in leaves of S. Rebaudiana Bertoni grown in Mexico by a validated HPLC method.Am. J. Analyt. Chem., 6:878-888.
Goyal, S. K., Samsher and Goyal, R. K. (2010). Stevia (Stevia rebaudiana) a bio-sweetener: a review. Int. J. Food Sci. Nutr.,61: 1–10.
Hansen, H.J. (2010). Future use of steviol glycosides: results of a survey in the German beverage industry. J.
Verbrauch. Lebensm., 5: 237–239.
Hashimoto, Y., Moriyasu, M., Nakamura, S., Ishiguro, S. and Komuro, M. (1978).High performance liquid
chromatographic determination of Stevia components on a hydrophilic packed column. J. Chromatogr, 161: 403-405.
He, B., Liu, Y. and Shi, Z. (1994). Application of an adsorbant AB-8 in extraction and separation of stevioside. Huase Yingyong, 11: 16-19.
Hearn, L. and Subedi, P. (2009). Determining levels of steviol glycosides in the leaves of Stevia rebaudiana by near infrared reflectance spectroscopy. J. Food Compos. Anal., 22: 165–168.
Herranz, L.M., Barrajon, C.E., Beltran, D.R., Joven, J. and Micol, V. (2010). Stevia is a source for alternative sweeteners: potential medicinal effects. Agro Food Ind. Hi Tec, 21: 38–42.
Hoekstra, B., Traub, J., Chamberlain, K., Baugh, S. and Venkataraman, S.K. (2009).Comparative study of HPLC methods for the analysis of diterpene glycosides from Stevia rebaudiana. Planta Med., 75: 1003-1013.
Hossain, M.A., Siddique, A.B., Rahman, S.M.M. and Hossain, M.A. (2010). Chemical composition of the essential oils of Stevia rebaudiana Bertoni leaves. Asian J. Tradit. Med., 5: 56-61.
Hsieh, M.H., Chan, P., Sue, Y.M., Liu, J.C., Liang, T.H., Huang, T.Y., Tomlinson, B., Chow, M.S., Kao, P.F. and Chen, Y.J. (2003). Efficacy and tolerability of oral stevioside in patients with mild essential hypertension: a two-year, randomized, placebo-controlled study. Clin.Ther., 25: 2797-2808.
Huang, X.Y., Fu, J.F. and Di, D.L. (2010). Preparative isolation and purification of steviol glycosides from Stevia rebaudiana Bertoni using high speed counter current chromatography. Sep. Purif. Technol., 71: 220-224.
Huber, J., Borie, N., Chollet, S., Perret, J., Barbet, M. C., Berger, M., Dayde, J. and Renault, J.H. (2015).
Intensified separation of steviol glycosides from a crude aqueous extract of Stevia rebaudiana leaves using
centrifugal partition chromatography. Planta Med., 81: 1614-1620.
Ibrahim, M.A., Rodenburg, D.L., Alves, K., Perera, W.H., Fronczek, F.R., Bowling, J. and McChesney, J.D. (2016). Rebaudiosides R and S, minor diterpene glycosides from the leaves of Stevia rebaudiana. J. Nat. Prod., 79:1468-1472.
Inamake, M.R., Shelar, P.D., Kulkarni, M.S., Katekar, M.S. and Tambe, R. (2010). Isolation and analytical
characterization of stevioside from leaves of Stevia rebaudiana Bert; Asteraceae. Int. J. Res. Ayurveda Pharm., 1: 572-581.
Ishizone, H. (1979). Separation and purification of stevioside. Japan Kokai Tokkyo Koho, 79: 400.
Jackson, A., Tata, A., Wu, C., Perry, R., Haas, G., West, L. and Cooks, R.G. (2009). Direct analysis of stevia leaves for diterpene glycosides by desorption electrospray ionization mass spectroscopy. Analyst, 1345: 867–874.
Jadhao, D.B., Katekhaye, S.D. and Thorat, B.N. (2011).Improved RP-HPLC method for quantitative estimation of stevioside in Stevia rebaudiana Bertoni Burm.Int. J. Phyto. Pharm., 1: 27-34.
Jaitak, V., Bandna, Singh, B. and Kaul, V.K. (2009).An efficient microwave-assisted extraction process of
stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni).Phytochem. Analysis, 20: 240–245.
Javad, S., Naz, S., Ilyas, S., Tariq, A. and Aslam, F. (2014).Optimization of the microwave assisted extraction and its comparison with different conventional extraction methods for isolation of stevioside from Stevia rebaudiana. Asian J. Chem., 26: 8043-8048.
Jaworska, K., Krynitsky, A.J. and Rade, R.J.I. (2012). Simultaneous analysis of steviol and steviol glycosides by liquid chromatography with ultraviolet detection on a mixed-mode column: application to stevia plant material and stevia-containing dietary supplements. J. AOAC Int.,95: 1588-1596.
JECFA (Joint FAO/WHO Expert Committee on Food Additives).(2010). Steviol glycosides.Compendium of food addditive specifiations, 73th meeting, FAO JECFA monographs 10, pp.17−22.
Jeppesen, P., Gregersen, S., Gregersen, S., Alstrupp, K. and Hermansen, K. (2002). Stevioside induces
antihyperglycaemic, insulinotropic and glucagonostatic effects in vivo: studies in the diabetic Goto-Kakizaki (GK) rats. Phytomedicine, 9: 9–14.
Jonnala, K.K., Kiran, B.G., Kaul, V.K. and Ahuja, P.S. (2006).Process for production of steviosides from Stevia rebaudiana Bertoni.US patent 0142555.
Kakigi, Y., Suzuki, T., Icho, T., Uyama, A. and Mochizuki, N. (2013).Classification of stevia sweeteners in soft drinks using liquid chromatography and time-of-flight mass spectroscopy.Food Addit.Contam. A,30: 2043–2049.
Kamauchi, H., Kon, T., Kinoshita, K., Takatori, K. and Takahashi, K. (2015). New diterpenoids isolated from Stevia rebaudiana fermentedby Saccharomyces cerevisiae. Tetrahedron let.,56: 4377-4382.
Kasai, R., Yamaguchi, H. and Tanaka, O. (1987). High-performance liquid chromatography of glycosides on a new type of hydroxyapatite column. J. Chromatogr., 407: 205–210.
Katayama, O., Sumida, T., Hayashi, H. and Mitsuhashi, H. (1976). The practical application of stevia and research and development data.Japan: I.S.U. Company, 747p.
Kaur, G., Pandhair, V. and Cheema, G.S. (2014). Extraction and characterization of steviol glycosides from Stevia rebaudiana Bertoni leaves. J. Med. Plants. Stud., 2: 41-45.
Kaushik, R., Narayanan, P., Vasudevan, V., Muthukumaran, G. and Antony, U. (2010). Nutrient composition of cultivated stevia leaves and the influence of polyphenols and plant pigments on sensory and antioxidant properties of leaf extracts. J. Food Technol., 47: 27-33.
Kedik, S.A., Yartsev, E.I. and Stanishevskaya, I.E. (2009).Antiviral activity of dried extract ofStevia.Pharm. Chem. J.,43: 198–199.
Khaled, A., Shams, N.S., Azim, A., Saleh, I.A., Hegazy, M.E.F., Missiry, M.M. and Hammouda, F.M. (2015). Green technology: economically and environmentally innovative methods for extraction of medicinal & aromatic plants (MAP) in Egypt. J. Chem. Pharm. Res., 7: 1050-1074.
Khoddami, A., Wilkes, M.A. and Roberts, T.H. (2013).Review-techniques for analysis of plant phenolic compounds.Molecules, 18: 2328-2375.
Kienle, U. (1990). Method of making a natural sweetener based on Stevia rebaudiana, and use thereof. Int. Cl.5 A61K 35/78. U.S. Cl. 424/195.1, 586690 Sep. 24, 1990. Chemical abstracts No. 111, 22442z.
Kienle, U. (2007). Stevia rebaudiana natural sweetness in the bureaucratic jungle. J. Culinaire, 5: 59–69.
Kienle, U. (2010). A natural fabric makes career. J. Verbrauch. Lebensm.,5: 199–203.
Kinghorn, A.D. and Soejarto, D.D. (1985).Current status of stevioside as a sweetening agent for human use. In: Economic and medicinal plant research, vol.1. (eds. Wagner, H., Hikino, H. and Farnsworth, N.R.)London, New York Academic Press.pp. 1-52.
Kinghorn, A.D., Nanayakkara, N.P.D., Soejarto, D.D., Medon, P.J. and Kamath, S. (1982). Potential sweetening agents of plant origin: I. Purification of Stevia rebaudiana sweet constituents by droplet counter-current chromatography. J. Chromatogr., 237: 478-483.
Kinghorn, A.D. (2002). Overview.Stevia: the Genus Stevia (Medicinal and Aromatic Plants-Industrial Profiles.(ed. Kinghorn, A.D.). Taylor and Francis: London ,pp.1−17.
Kitada, Y., Sasaki, M. and Yamazoe, Y. (1989).Simultaneous determination of stevioside, rebaudioside A and C and dulcoside A in foods by high-performance liquid chromatography.J. Chromatogr, 474: 447-451.
Kohda, H., Kasai, R., Yamasaki, K., Murakami, K. and Tanaka, P. (1976).Steviosides from Stevia rebaudiana Bertoni.Phytochemistry, 15: 981–982.
Kolb, N., Herrera, J.L., Ferreyra, D.J. and Utiana, R.F. (2001). Analysis of sweet diterpene glycosides from Stevia rebaudiana: Improved HPLC method. J. Agr. Food Chem., 49: 4538-4541.
Koshiro, C.S.K.K. (1980).Stevia components as sweetening agents and antibiotics. Japan Patent 55-92323.
Kroyer, G. (2010). Stevioside and stevia-sweetener in food: application, stability and interaction with food ingredients. J. Verbrauch. Lebensm.,5: 225–229.
Kubica, P., Namiesnik, J. and Wasik, A. (2015). Determination of eight artificial sweeteners and common Stevia rebaudiana glycosides in non-alcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectroscopy. Anal.Bioanal. Chem., 407: 1505–1512.
Kumari, M. and Chandra, S. (2015).Phytochemical studies and estimation of major steviol glycosides in varied parts of Stevia rebaudiana.Int. J. Pharm. Pharm. Sci., 7: 62-65.
Kumari, N., Rana, R.C., Sharma, Y.P. and Kumar, S. (2016a). Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni. JANS., 8: 1953-1958. 1953-1958.
Kumari, N., Rana, R.C., Sharma, Y.P. and Kumar, (2016b).Variations in stevioside and rebaudioside-A content in Stevia rebaudiana under different growing conditions. Int. J. Agr. Sci., 8: 1329-1331.
Kuo, W.S. and Chiang, B.H. (1987). Recovery of glutamic acid from fermentation broth by membrane processes. J. Food Sci., 52: 1401.
Kuroda, A. and Kamiyama, S. (1979). Stevia leaf extract purification. Patent‐Japan Kokai Tokkyo Koho‐79 76,600: 3.
Lasekan, O. and Naidu, K.R.M. (2013). Changes in the volatile constituents of the leaves of Stevia rebaudiana Bertoni caused by different drying procedures. J. Food Agric. Environ., 11: 190-194.
Lee, C.N., Wong, K., Liu, J., Chen, Y., Chen, J. and Chan, P. (2001). Inhibitory effect of stevioside on calcium influx to produce anti-hypertension. Planta Med., 67: 796-799.
Li, J., Chen, Z., and Duolong, D. (2012). Preparative separation and purification of rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins. Food Chem., 132: 268–27.
Liu, J., Ong, C.P. and Li, S.F.Y. (1997). Subcritical fluid extraction of stevia sweeteners from Stevia rebaudiana. J. Chromatogr. Sci., 35: 446- 450.
Lorenzo, C., Diaz, J.S., Plaza, M., Quintanilla, C. and Alonso, G.L. (2014). Fast methodology of analysing major steviol glycosides from Stevia rebaudiana leaves. Food Chem., 157: 518–523.
Madan, S., Ahmad, S., Singh, G.N., Kohli, K., Singh, Y.K.R. and Garg, M. (2010). Stevia rebaudiana (Bert.)Bertoni- a review. Indian J. Nat. Prod.Resour.,1: 267-286.
Magomet, M., Tomov, T., Somann, T. and Abelyan, V.H. (2011).Process for manufacturing a sweetener and use thereof. Google patents 7,862,845B2.
Mantovaneli, I.C.C., Ferretti, E.C., Simoes, M.R. and Silva, F.C. (2004).The effect of temperature and flow rate on the clarification of the aqueous stevia-extract in a fixed-bed column with zeolites. Braz. J. Chem. Eng., 21: 449-458.
Markovic, I.S., Darmati, Z.A. and Abramovic, B.F. (2008). Chemical composition of leaf extract of Stevia rebaudianaBertoni grown experimentally in Vojvodina. J. Serb. Chem. Soc., 73: 283-297.
Martono, Y., Riyanto, S., Rohman, A., Martono, S. (2016). Effect of mobile phase composition, organic modifier and flow rate on selectivity and retention of stevioside and rebaudioside Aon isocratic RP-HPLC analysis. IJPCR, 8: 397-402.
Masuyama, F. (1980). Stevioside sweetener purification from Stevia rebaudiana leaves. Japan Kokai Patent 80,07,039.
Mauri, P., Catalano, G., Gardana, C. and Pietta, P. (1996). Analysis of stevia glycosides by capillary electrophoresis. Electrophoresis, 17: 367-371.
Mc Garvey, B.D., Attygalle, A.B., Starratt, A.N., Xiang, B., Schroeder, F.C., Brandle, J.E. and Meinwald, J. (2003). New non-glycosidic diterpenes from the leaves of Stevia rebaudiana. J. Nat. Prod., 66: 1395-1398.
Meneni, S. and Chaturvedula, V.S.P. (2015). Continuation of reversed-phase HPLC analysis studies of steviol glycosides isolated from Stevia rebaudiana Bertoni. J. Food Res., 4: 87-91.
Minne, V.J., Compernolle, F., Toppet, S. and Geuns, J.M. (2004). Steviol quantification at the picomole level by high-performance liquid chromatography. J. Agr. Food Chem., 52: 2445–2449.
Mishra, P., Singh, R., Kumar, U. and Prakash, V. (2010). Stevia rebaudiana – a magical sweetener. GJBB, 5: 62–74.
Mitchell, H.L. (2006). Sweeteners and sugar alternatives in food technology. Blackwell Publishing Ltd., Oxford, UK, pp. 341-347.
Mohan, K. and Robert, J. (2009). Hepatoprotective effects of Stevia rebaudiana Bertoni leaf extract in CCl4-induced liver injury in albino rats. Med. Aromat. Plant Sci. Biotechnol., 3: 59–61.
Mondaca, R.L., Galvez, A.G., Bravo, L.Z. and Hen, K. (2012). Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: a comprehensive review on the biochemical, nutritional and functional aspects. Food Chem., 132: 1121–1132.
Musa, A., Gasmalla, M.A.A., Miao, M., Zhang, T., Aboshora, W., Eibaid, A. and Jiang, B. (2014). Separation and structural characterization of tri-glucosyl-stevioside. J. Acad. Indus. Res., 2: 593-598.
Nikolova, D. B., Bankova, V. and Popov, S. (1994). Separation and quantification of stevioside and rebaudioside-A in plant extracts by normal phase high performance liquid chromatography and thin layer chromatography: a comparison. Phytochem. Analysis, 5: 81-85.
Oddone, B. (1999). How to grow stevia.Guarani Botanicals, Inc., Pawcatuck, Connecticut, pp. 1-30.
Okane, H. and Kamata, L. (1977). Latino wit and wisdom. Japan Patent 52,057,198.
Oshima, Y., Saito, J. and Hikino, H. (1986). Sterebins A, B, C and D, bis norditerpenoids of Stevia rebaudiana leaves. Tetrahedron, 42: 6443-6446.
Oshima, Y., Saito, J. and Hikino, H. (1988). Sterebins E, F, G and H, diterpenoids of Stevia rebaudiana leaves. Phytochemistry, 27: 624-626.
Pasquel, A., Meireles, M.A.A., Marque, M.O.M. and Petenate, A.J. (2000). Extraction of stevia glycosides with Co2 + water, Co2 + ethanol, and Co2 + water + ethanol. Braz. J. Chem. Eng., 17:271-282.
Payzant, J.D., Laidler, J.K. and Ippolito, R.M. (1999). Method of extracting selected sweet glycosides from the Stevia rebaudianaplant. U.S. Patent 5,962,678.
Phillips, K.C. (1987). Stevia: steps in developing a new sweetener. Developments in Sweeteners.(ed. Grenby, T.H.). Elsevier Applied Science London, pp. 1–43.
Piasecka, A., Krzeminnska, I. and Tys, J. (2014). Physical methods of microalgal biomass pretreatment.Int. Agrophys., 28: 341-348.
Pieri, V., Belancic, A., Morales, S. and Stuppner, H. (2011).Identification and quantification of major steviol glycosides in Stevia rebaudiana purified extracts by 1H NMR spectroscopy. J. Agr. Food Chem., 59: 4378–4384.
Pol, J., Ostra, E.V., Karasek, P., Roth, M., Benesova, K., Kotlarikova, P. and Caslavsky, J. (2007). Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana: methanol versus water. Anal. Bioanal. Chem., 388: 1847-1857.
Prakash, I., Bunders, C., Devkota, K.P., Charan, R.D., Ramirez, C., Priedemann, C. and Markosyan, A. (2014a). Isolation and Characterization of a novel rebaudioside M isomer from a bioconversion reaction of rebaudioside A and NMR comparison studies of rebaudioside M isolated from Stevia rebaudiana Bertoni and Stevia rebaudiana morita. Biomolecules, 4:374-389.
Prakash, I., Campbell, M. and Chaturvedula, V.S.P. (2012). Catalytic hydrogenation of the sweet principles of Stevia rebaudiana, rebaudioside B, rebaudioside C and rebaudioside D and sensory evaluation of their
reduced derivatives. Int. J. Mol. Sci., 13: 15126-15136.
Prakash, I., Clos, J.F. and Chaturvedula, V.S.P. (2011). Stability of steviol glycosides in mock beverages under acidic conditions. IJPSR, 3: 421-425.
Prakash, I., Dubois, G., Clos, J., Wilkens, K. and Fosdick, L. (2008). Development of rebiana, a natural, non-caloric sweetener. Food Chem. Toxicol., 46:575–582.
Prakash, I., Markosyan, A. and Bunders, C. (2014b). Development of next generation stevia sweetener:
rebaudioside M. Foods, 3: 162-175.
Puri, M., Sharma, D. and Tiwari, A.K. (2011). Downstream processing of stevioside and its potential applications. Biotechnol. Adv., 29: 781-789.
Puri, M., Sharma, D., Barrow, C.J. and Tiwari, A.K. (2012). Optimisation of novel method for the extraction of
steviosides from Stevia rebaudiana leaves. Food Chem., 132: 1113-1120.
Purkayastha, S., Markosyan, A. and Malsagov, M. (2012). Process for manufacturing a sweetener and use thereof. Patents 8,334,006.
Rajab, R., Mohankumar, C., Murugan, K., Harish, M. and Mohanan, P.V. (2009). Purification and toxicity studies of stevioside from Stevia rebaudiana Bertoni. Toxicol. Int., 16: 49-54.
Rajasekaran, T., Ramakrishna, A., Sankar, U.K., Giridhar, P. and Ravishankar, G.A. (2008). Analysis of predominant stevioside in Stevia rebaudiana Bertoni by liquid chromatography/electrospray ionization-mass spectroscopy. Food Biotechnol., 22: 179–188.
Ramesh, K., Singh, V. and Megeji, N.W. (2006). Cultivation of Stevia (Stevia rebaudiana Bertoni): a comprehensive review. Adv. Agron., 89: 137−177.
Rao, A.B., George, S.A., Alavala, S., Meshram, H.M. and Shekar, K.C. (2015). Metal salts assisted enzyme-based extraction of stevioside from the leaves of Stevia rebaudiana Bertoni. Adv. Biosci. Biotechnol., 6: 734-743.
Rao, A.B., Prasad, E., Roopa, G., Sridhar, S. and Yerrapragada, V.L.R. (2012). Simple extraction and membrane purification process in isolation of steviosides with improved organoleptic activity. Adv. Biosci. Biotechnol., 3: 327-335.
Reshu, G., Vidushi, Y. and Manvi, R. (2014). A review on importance of natural sweetener, a zero caloric
plant- stevia- having medicinal and commercial importance. IJFANS, 3:90-94.
Rodenburg,D.L., Alves, K., Perera, W.H., Ramsaroop, T., Carvalho, R. and McChesney, J.D. (2016).
Development of HPLC analytical techniques for diterpene glycosides from Stevia rebaudiana (Bertoni) Bertoni: strategies to scale-up. J. Brazil. Chem. Soc., 27: 1406-1412.
Roy, A. and De, S. (2014). Extraction of steviol glycosides using novel cellulose acetate pthalate (CAP)- polyacrylonitrile blend membranes. J. Food Eng., 126: 7-16.
Saifi, M., Ali, A., Saini, M., Nasrullah, N., Khan, S. and Abdin, M.Z. (2014). A rapid and efficient high performance thin layer chromatographic (HPTLC) method for simultaneous analysis of stevioside and rebaudioside-A in Stevia rebaudiana. Int. J. Pharm. Pharm. Sci., 6: 455-462.
Samah, N.A., Hisham, A.D.A. and Rahim, S.A. (2013). Determination of stevioside and rebaudioside A in Stevia rebaudiana leaves via preparative high performance liquid chromatography (prep-HPLC). IJCEE, 4: 86-89.
Satishkumar, J., Sarvanan, M.M. and Seethalakshmi, I. (2008). In-vitro antimicrobial and antitumor activities of Stevia rebaudiana (Asteraceae) leaf extracts. Trop. J. Pharm. Res., 7: 1143–1149.
Schiffman, S.S., Miller, S.E.A., Graham, B.G., Bennett, J.L., Booth, B.J. and Bishay, D.N. (2000). Effect of temperature, pH, and ions on sweet taste. Physiol. Behav., 68: 469–481.
Serio, L. (2010). La Stevia rebaudiana, une alternative au sucre. Phytotherapie, 8: 26–32.
Shah, R., Jager, L.S. and Begley, T.H. (2012). Simultaneous determination of steviol and steviol glycosides by liquid chromatography-mass spectroscopy. Food. Addit. Contam. A., 29: 1861-1871.
Sharma, M., Thakral, N.K. and Thakral, S. (2009). Chemistry and in vivo profile of ent- kaurene glycosides of Stevia rebaudiana Bertoni- an overview. Nat. Prod. Radiance, 8: 181-189.
Shock, C.C. (1982). Experimental cultivation of rebaudi’s Stevia in California. University of California, Davis. AgronomyProgress Report No. 122, 9p.
Silva, F.V., Bergamasco, R., Andrade, C.M.G., Pinheiro, N., Machado, N.R.C.F., Reis, M.H.M., Araujo, A.A. and Rezende, S.L. (2007). Purification process of stevioside using zeolites and membrane. Int. J. Chem. React. Eng., 5: 1-6.
Silva, P.A., Oliveira, D.F., Prado, N.R., Carvalho, D.A. and Carvalho, G.A. (2008). Evaluation of the antifungal activity by plant extracts against Colletotrichum gloeosporioides PENZ. Cienc. Agrotec., 32: 420–428.
Soejarto, D.D. (2002). Ethnobiology of stevia and Stevia rebaudiana. Stevia: the genus Stevia (Medicinal and Aromatic Plants – Industrial Profiles). (ed. Kinghorn A.D.), Taylor and Francis/CRC Press, New York/London, UK. pp. 40–67.
Surana, S.J., Gokhale, S.B., Rajmane, R.A. and Jadhav, R.B. (2006). Nonsaccharide natural intense sweeteners: an overview of current status. Nat. Prod. Radiance, 5: 270-278.
Takahashi, K., Iwata, Y., Mori, S. and Shigeta, S. (1998). In-vitroanti-HIV activity of extract from Stevia rebaudiana. Antivir. Res., 37: A59.
Takahashi, K., Matsuda, M., Ohashi, K., Taniguchi, K., Nakagomi, O., Abe, Y., Mori, S., Sato, N., Okutani, K. and Shigeta, S. (2001). Analysis of anti-rotavirus activity of extract fromStevia rebaudiana. Antivir. Res., 49: 15–24.
Takamura, K., Kawaguchi, M. and Isono, C. (1977). Separation of stevioside. Japan Kokai 77-51069.
Tavarini, S. and Angelini, L.G. (2013). Stevia rebaudiana Bertoni as a source of bioactive compounds: the effect of harvest time, experimental site and crop age on steviol glycoside content and antioxidant properties. J. Sci. Food. Agr.,93: 2121–2129.
Teo, C.C., Tan, S.N., Yong, J.W.H., Hew, C.S. and Ong, E.S. (2009). Validation of green-solvent extraction combined with chromatographic chemical fingerprint to evaluate quality of Stevia rebaudiana Bertoni. J. Sep. Sci.,32: 613-622.
Tiwari, S., Arnold, R., Saxena, A., Tiwari, A., Mishra, N., Awasthi, S. and Tiwari, S. (2014). Evaluation of extraction of stevioside and rebaudioside from Stevia rebaudiana bertoni by pressurized hot water extraction. SSJBT, 3: 153-158.
Vanek, T., Nepovim, A. and Valicek, P. (2001). Determination of stevioside in plant material and fruit teas. J. Food. Compos. Anal., 14: 383-388.
Vanneste, J., Sotto, A., Courtin, C.M., Van, C.V., Bernaerts, K., Van, I.J., Vandeur, J., Taes, S. and Vander, B.B. (2011). Application of tailor-made membranes in a multi-stage process for the purification of sweeteners from Stevia rebaudiana. J. Food. Eng., 103: 285–293.
Wang, Y.H., Avulaa, B., Tanga, W., Wanga, M., Elsohlya, M.A. and Khan, I.A. (2015).Ultra-HPLC method for quality and adulterant assessment of steviol glycosides sweeteners- Stevia rebaudiana and stevia products. Food. Addit. Contam. A., 32: 674–685.
Woelwer, R.U., Lankes, C., Wawrzun, A. and Wuest, M. (2010). Improved HPLC method for the evaluation of the major steviol glycosides in leaves of Stevia rebaudiana. Eur. Food. Res. Technol., 231: 581–588.
Woelwer, R.U. (2012). The leaves of Stevia rebaudiana (Bertoni), their constituents and the analyses thereof: a review. J. Agr. Food. Chem., 60: 886−895.
Wolwer, R., Tomberg, W. and Wawrzun, A. (2010). Investigations on the stability of stevioside and rebaudioside A in soft drinks. J. Agr. Food. Chem., 58: 12216−12220.
Yabu, R., Takase, M., Toda, K., Tanisoto, K., Yasutake, A. and Iwaeoto, Y. (1977). Studies on stevioside, natural sweetener: effect on the growth of some oral microorganisms. Hiroshima Daigaku Shigaku Zasshi, 9: 12-17.
Yadav, A.K., Singh, S., Dhyani, D. and Ahuja, A.S. (2011). A review on the improvement of Stevia. Can. J. Plant. Sci., 91: 1-27.
Yadav, S.K. and Guleria, P. (2012). Steviol glycosides from stevia: biosynthesis pathway review and their application in foods and medicine. Crit. Rev. Food. Sci. Nutr., 52: 988-98.
Yoda, S.M., Marques, M.O.M., Petenate, A.J. and Meireles, M.A.A. (2003). Supercritical fluid extraction from Stevia rebaudiana Bertoni using CO2 and CO2+water: extraction kinetics and identification of extracted components. J. Food Eng., 57:125−134.
Yu, Z.R. and Chiang, B.H. (1986). Passion fruit juice concentration by ultra filtration and evaporation. J. Food Eng., 51: 1501.
Zhang, S.Q., Kumar, A. and Kutowy, O. (2000). Membrane-based separation scheme for processing sweeteners from stevia leaves. Food. Res. Int., 33: 617–620.
Zimmermann, B.F., Woelwer, R.U. and Papagiannopoulos, M. (2011).Separation of steviol glycosides by hydrophilic liquid interaction chromatography. Food Anal. Methods, 5: 266-271.
Zlabur, J.S., Voca, S., Dobricevic, N., Brncic, M., Dujmic, F. and Brncic, S.R. (2015). Optimization of ultrasound assisted extraction of functional ingredients from Stevia rebaudiana Bertoni leaves. Int. Agrophys., 29: 231-237.
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Kumari, N., & Kumar, S. (2017). Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review. Journal of Applied and Natural Science, 9(4), 2114-2126. https://doi.org/10.31018/jans.v9i4.1497
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