Study of chromosomal abnormalities in Channa punctatus exposed to fenvalerate
Article Main
Abstract
The chromosomal aberration test in kidney cells of Channa punctatus was conducted to study the genotoxic effect of synthetic pyrethroid fenvalerate, Different chromosomal abnormalities were seen and their frequency was recoded in metaphase spreads obtained from fishes exposed to three sub lethal concentrations (0.0625, 0.0314 and 0.0157 ppm) of this compound. The exposure to fenvalerate caused various structural abnormalities in chromosomes such as chromatid break, fragment, gap, chromatid separation, deletion and ring type chromosomes. Results obtained in present investigations clearly indicate that fenvalerate acts as a genotoxic agent in Channa punctatus.
Article Details
Article Details
Keywords
Fenvalerate, Chromosomal aberration, Genotoxic effect, Channa punctatus
References
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Seth, N. and Saxena, K.K. (2003). Haematological responces in a fresh water fish Channa punctatus to experimental fenvalerate poisoning. Bull. Environ. Contam. Toxicol., 71: 1192-1199.
Sirohi, V. and Saxena, K.K. (2006). Toxic effect of ë-cyhalothrin on biochemical contents of fresh water fish Channa punctatus. J. Fisheries Aquatic Sci., 1(2): 112-116
Al-Sabti, K. and Metcalfe, C.D. (1995). Fish micronuclei for assessing genotoxicity in water. Mutat. Res., 343: 121-135.
Alderic, D.F. (1967). Detection and measurement of water pollution biological assay. Canadian Fisheries, 9: 33-39.
Ali, D., Nagpure, N.S., Kumar, S., Kumar, R., Kushwaha, B. and Lakra, W.S. (2009) Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food Chem. Toxicol., 47(3):650-656.
Ali, F.K., El-Shehawi, A.M. and Seehy, M.A. (2008). Micronuleus test in fish genome: a sensitive monitor for aquatic pollution. African J. Biotech., 7(5): 606-612.
Arokia-Rita, J.J. and Selvanayagam, M. (1998). Genotoxic effect of fenvalerate on the chromosomes of fish Oreochrochromis mossambicus (Peter). Poll. Res., 17(2): 119-122.
Asano, Y.F.N., Sifuni, T. and Ojima, Y. (1998). Development of genotoxic assay systems that use aquatic organisms. Mautat. Res., 399: 125-133.
Bond, G.G. and Rossbacher, R. (1993). A review of potential human carcinogenecity of the chlorophenoxy herbicides. MCPA, MCPP and 2, 4-DP. Br. J. Ind. Med., 50:340-348.
Chauhan, L.K.S., Pant, N., Gupta, S.K. and Srivastava, S.P. (2000). Induction of chromosomal aberrations, micronucleus formation and sperm abnormalities in mouse following of carbofuran exposure. Mut. Res., 465: 123-129.
Devi, K.D.; Rozati, R.; Banu, B.S.; Jamil, K. and Grover, P. (2001). In vivo genotoxic effect of potassium dichromate in mice leukocytes using comet assay. Food Chem. Toxicol., 39: 859-865.
Eisler, R. (1992). Fenvalerate hazards to fish, wildlife and invertebrates. A synoptic review. Biological report fish and wildlife service. US Department of the interior. 2.111 + 43pp. Contaminant Hazard Reviews, Report. 24.
Grisolia C.K. and Cordeiro CMT (2000). Variability in micronucleus induction with different mutagens applied to several species of fish. Genetics and Molecular Biology, 23: 235-239.
Lioi, M.B.; Santoro, A.; Barbieri, R., Salzano, S. and Ursine, M.V. (2004). Ochratoxin A and Zearalenone : a comperative study on genotoxic effects and cell death induced in bovine lymphocyte, Mutation Res., 1: 19-27.
Manna, G.K. and Mukherjee, P.K. (1986). Effect of organophosphate insecticide melathion on chromosome, cell division and total muscle proteins of the cichlid fish Tilapia. In Pers. Cytol. and Genet. (Eds. G.K. Manna and U. Sinha) 5: 225-235.
Mathew, A., N.P. and Jahageerdar, S. (1999). Effect of heavy metal on the karyotype of Channa punctatus. Indian J.Fish, 46(2): 167-172.
Matsumura, F. (1985). In: Toxicology of insecticides, 2nd ed. Plenum Press, New York.
Nagpure, N.S. and Barat, A. (1997). A simplified method of fish chromosome preparation by in vitro colchicine treatment. Ind. J.Exp. Biol., 35:915-916.
Ojima, Y. (1982). Method in fish cytogenetics Nucleus. 25: 1-7.
Pimental, D. and Levitan, L. (1986). Pesticides-Amounts applied and amounts reaching pests. Biosciences. 36: 86-91.
Poli, P., Mello, M.A., de B Guschini, A.; Castro, V.L.S.S.; de Restivo, F.M., Rossi, C., Zocchi, T.M.A.D., de Mello, M.A. and de Castro V.L.S.S. (2003). Evaluation of the genotoxicity induced by fungicide fenarimol in mammalian and plant cells by use of single cell gel electrophoresis assay, Mutation Res. 540: 57-66.
Rishi, K.K. and Haobam, M.S. (1990). A chromosomal study on four species of snake haeds (Ophicoephalidae, Pisces) with comments on their Karyotype evolution. Caryologia, 43: 163-163.
Rishi, K.K. and Grewal, S.. (1995). Chromosome aberration test for the insecticide, dichlorvos, of fish chromosomes. Mutat. Res., 344: 1-4.
Saxena, K.K. and Rana, R. (2005). Genotoxic effect of cypermethrin in a fresh water fish Channa punctatus. Proceedings of International Transfer of Technology Initiative Workshop on Parasitology and Genotoxicity in Sustainable Aquaculture, Allahabad : 26.
Saxena, K.K. and Rana, R. (2008). Genotoxicity of permethrin in Channa punctatus by using chromosomal aberration test. Proceedings of 78th Annual Session and Symp. Nat. Acad. Sci., India. 41.
Saxena, K.K. and Seth, N. (2002). Toxic effect of cypermethrin on certain hematological aspects of fresh water fish Channa punctatus. Bull. Environ. Contam. Toxicol., 64: 364-369.
Saxena, K.K. and Sirohi, V. (2007). Effect of ë-cyhalothrin on the activities of trypsin and lipase in fresh water fish Channa punctatus. J. Fish. Aquatic Sci., 2(2): 168-172.
Seth, N. and Saxena, K.K. (2003). Haematological responces in a fresh water fish Channa punctatus to experimental fenvalerate poisoning. Bull. Environ. Contam. Toxicol., 71: 1192-1199.
Sirohi, V. and Saxena, K.K. (2006). Toxic effect of ë-cyhalothrin on biochemical contents of fresh water fish Channa punctatus. J. Fisheries Aquatic Sci., 1(2): 112-116
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Study of chromosomal abnormalities in Channa punctatus exposed to fenvalerate. (2010). Journal of Applied and Natural Science, 2(1), 70-73. https://doi.org/10.31018/jans.v2i1.99
