Effect of super-optimal levels of fertilizers on soil enzymatic activities during growth stages of wheat crop on an Inceptisol
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Abstract
A field experiment was conducted during 2010-2011 and 2011-2012 to investigate the effect of optimal (100% NPK) to super-optimal doses (200% NPK) of mineral fertilizers on soil enzymes such as dehydrogenase (DHA), acid phosphatase (Ac-PA), alkaline phosphatase (Alk-PA), fluorescien diacetate hydrolysis (FDA), urease and nitrate reductase (NRA) at three physiological stages (CRI, anthesis and maturity) of wheat crop on an Inceptisol. Dehydrogenase activity was reduced by 28-37% when fertilizer application was at super-optimal dose (200% NPK), whereas, urease and NRA responded positively in the range of 43-44% and 213-231% respectively. Alk-PA
was 7.3-7.9% higher in treatments receiving 125% NPK as compared to control (100% NPK); whereas, Ac-PA declines in the plots receiving 175 and 200% of recommended dose of fertilizer (RDF) as compared to 150% NPK levels. Addition of 175% RDF increased the FDA to the tune of 46-53% as compared to 100% NPK. A significant (P?0.05) positive interaction between fertilizer treatments and physiological stages of wheat growth was observed on soil enzyme activities (except urease and NRA) being highest at the anthesis stage of wheat. Correlation matrix analysis showed that DHA was correlated with the studied enzyme activities except Ac-PA and FDA; whereas, strong correlation was observed between urease and NRA (r=0.981, P=0.01). This study provides theoretical and practical base for avoiding super optimal application of fertilisers which hinders the enzyme activities and vis-a-vis sustainable nutrient enrichment under rhizosphere.
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Article Details
Physiological stages, Soil enzymes, Super-optimal fertilization, Wheat
Blaise, D., Singh, J.V., Bonde, A.N., Tekale, K.U. and Mayee, C.D. (2005). Effects of farmyard manure and fertilizers on yield, fibre quality and nutrient balance of rainfed cotton (Gossypium hirsutum). Bioresour. Technol. 96: 345-349.
Clarholm, M. (1993) Microbial biomass P, labile P, and acid phosphatase activity in the humus layer of a spruce forest, after repeated additions of fertilizers. Biol. Fertil. Soils 16:287–292
Douglas, L.A. and Bremner, J.M. (1971). A rapid method of evaluating different compounds as inhibitors of urease activity in soils. Soil Biol. Biochem. 3: 309-315.
Ge, G., Li, Z., Fan, F., Chu, G., Hou, Z. and Liang, Y. (2010). Soil biological activity and their seasonal variations in response to long-term application of organic and inorganic fertilizers. Plant Soil. 326: 31-44.
Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research. John Wiley and Sons, New York.
Graham, M.H. and Haynes, R.J. (2005). Organic matter accumulation and fertilizer-induced acidification interact to affect soil microbial and enzyme activity on a longterm sugarcane management experiment. Biol. Fert. Soils. 41: 249-256.
Green, V.S., Stott, D.E. and Diack, M. (2007). Assay of fluorescien diacetate hydrolytic activity: optimization for soil sample. Soil Biol. Biochem. 38: 693-701.
Harlook, K. (2006). PAU Honours, The Ludhiana Tribune, March 9, Chandigarh
Klein, D.A., Loh, T.C. and Goulding, R.L. (1971). A rapid procedure to evaluate dehydrogenase activity of soils low in organic matter. Soil Biol. Biochem. 3: 385-387.
Mandal, A., Patra, A.K., Singh, D., Swarup, A. and Masto, R.E. (2007). Effect of long-term application of manure and fertilizer on biological and biochemical activities in soil during crop development stages. Bioresour. Technol. 98: 3585-3592.
Mandal, A., Patra, A.K., Singh, D., Swarup, A., Purakayastha, T.J. and Masto, R.E. (2009). Effects of longterm organic and chemical fertilization on N and P in wheat plants and in soil during crop growth. Agrochimica. 53: 79-91.
Masto, R.E., Chhonkar, P.K., Singh, D. and Patra, A.K. (2006). Changes in soil biological and biochemical characteristics in a long-term field trial on a sub-tropical inceptisol. Soil Biol. Biochem. 38: 1577-1582.
Nayak, D.R., Babu, Y.J. and Adhya, T.K. (2007). Long-term application of compost influences microbial biomass and enzyme activities in a tropical Aeric Endoaquept planted to rice under flooded condition. Soil Biol. Biochem. 39: 1897-1906.
Okur, N., Altindisli, A., Cengel, M., Gocmez, S. and Kayikcioglu, H.H. (2009). Microbial biomass and enzyme activity in vineyard soils under organic and conventional farming systems. Turk. J. Agric. For. 33: 413-423.
Olander, L.P. and Vitousek, P.M. (2000). Regulation of soil phosphatase and chitinase activity by N and P availability. Biogeochemistry. 49: 175-190.
Patra, A.K., Abbadie, L., Clays-Josserand, A., Degrange, V., Grayston, S.J., Loiseau, P., Louault, F., Mahmood, S., Nazaret, S., Philippot, L., Poly, F., Prosser, J.I., Richaume, A. and Le Roux, X. (2005). Effect of grazing on microbial functional groups involved in soil N dynamics. Ecol. Monogr. 75: 65-80.
Quilchano, C. and Mara˜n´on, T. (2002). Dehydrogenase activity in Mediterranean forest soils. Biol. Fert. Soils. 35: 102-107.
Rakshit, R., Patra, A.K., Pal, D., Kumar, M. and Singh, R. (2012). Effect of elevated CO2 and temperature on nitrogen dynamics and microbial activity during wheat (Triticum aestivum L.) growth on a subtropical inceptisol in India. J. Agron. Crop Sci. 198: 452-465.
Rakshit, R., Patra, A.K., Purakayastha, T.J., Singh, R.D., Pathak, H., Dhar, S. and Das, A. (2016). Super-optimal fertilization affects root growth and soil microbial abundance and biomass during wheat growth. J. Food Agric. Environ. 14: 112-118.
Roberge, M.R. (1978). Methodology of soil enzyme measurement and extraction. In: R.G.Burn (Ed.), Soil Enzymes. Academic Press, pp.350.
Roldán, A., Salinas-García, J.R., Alguacil, M.M., Díaz, E. and Caravaca, F. (2005). Soil enzyme activities suggest advantages of conservation tillage practices in sorghum cultivation under subtropical conditions. Geoderma. 129: 178-185.
Saha, S., Gopinath, K.A., Mina, B.L. and Gupta, H.S. (2008). Influence of continuous application of inorganic nutrients to a maize–wheat rotation on soil enzyme activity and grain quality in a rainfed Indian soil. Eur. J. Soil Biol. 44: 521-531.
Sannino, F. and Gianfreda, L. (2001). Pesticide influence on soil enzymatic activities. Chemosphere. 45: 417-425.
Speir, T.W. and Cowling, J.C. (1991). Phosphatase activities of pasture plants and soils: relationship with plant productivity and soil P fertility induces. Biol. Fert. Soils. 12: 189-194.
Stark, C., Condron, L.M., Stewart, A., Di, H.J. and O'Callaghan, M. (2007). Effects of past and current crop management on soil microbial biomass and activity. Biol. Fert. Soils. 43: 531-540.
Trevors, J.T. (1984). Effect of substrate concentrations, inorganic NO3--N, temperature and pH on dehydrogenase activity in soil. Plant Soil. 77: 284-293.
Wen-Hui, Z., Zu-Cong, C. and He, Z. (2007). Effects of long term application of inorganic fertilizers on biochemical properties of a rice-planting red soil. Pedosphere. 17: 419-428.
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