Assessment of genetic diversity using DNA markers among Brassica rapa var. yellow sarson germplasm lines collected from Eastern Uttar Pradesh and Uttarakhand hills
Article Main
Abstract
The genetic diversity and the relatedness among thirty-one germplasm lines of yellow sarson collected from eastern UP were evaluated using morphological characters and Random Amplified Polymorphic DNA (RAPD) markers. Molecular parameters, viz. A total number of bands, average polymorphic band, average percent polymorphism, average polymorphic information content (PIC), Jaccard’s similarity coefficient, Principal Coordinate Analysis (PCA) and dendrogram generated using RAPD markers. A total of 148 different polymorphic amplification products were obtained using 10 selected decamer primers. The Jaccard similarity coefficient ranged from 0.557-0.899. Maximum polymorphism detected was 100 %.The range of amplification was from 190bp to 9 kb. Some unique bands were also reported with different primers that can be used for the identification of particular accession. PYSC-11-11 and PYSC-11-36 genotypes showed a maximum number of unique loci of different size. 31 germplasm lines grouped into two major clusters I and II based on RAPD profiling. Morphological characterization was done on the basis of leaf, petal and beak characteristics. The similarity value among the germplasm lines ranged from 0.222 to 1.000 using morphological descriptors. The dendrogram generated grouped the germplasm accession into two major groups at 44% similarity value. The cluster analysis was comparable up to some extent with Principal Coordinate Analysis (PCA) of two and three-dimensional plots. The variability revealed by morphological and molecular profile were found to be non-comparable. This study indicated the presence of high genetic diversity among collected yellow sarson germplasm, which could be used for developing for breeding and germplasm management purposes.
Article Details
Article Details
Brassica rapa, Genetic diversity, PCA, RAPD
Ahmad, J., Mohd, A., Ram Bhajan and Khan, G.T. (2009). Assessment of Genetic Diversity and Genetic relationships among twenty varieties of Brassica juncea [L.] using RAPD markers. International J. of Biotechnology and Biochemistry, 5(1): 85-92.
Beckman, J.S. and Soller, M. (1983). Restriction fragment length polymorphism in plant genetic improvement: methodologies, mapping and costs. Theoretical and Applied Genetics, 67: 35-43.
Chen, S., Zou, W.A. Cowling and Meng, J. (2010). Allelic diversity in novel gene pool of canola quality Brassica napus enriched with alleles from B. Rapa and B. carinata. Crop Pasture Science, 61: 483-492.
Demeke, T., Adams, R.P. and Chibber, R. (1992). Potential Taxonomic use of random amplified polymorphic DNA: A case study in Brassica. Theoretical and Applied Genetics, 84: 990-994.
Divaret, I. and Thomas, G. (1998). Use of RAPD markers to analyse the genetic variability of a collection of Brassica oleracea L. In Thomas G, Monteiro AA (eds.): Proc. Int. Symp. Brassicas. Acta Hort. 459: 255–262..
Geleta, N. and Labuschagne, M.T. (2005). Qualitative traits variation in sorghum (Sorghum bicolor (L.) Moench) germplasm from eastern highlands of Ethiopia. Biodiversity and Conservation, 14: 3055–64.
Ghosh, K.K., Haque, M.E., Parvin, M.S., Akhter, F. and Rahim, M.M. (2009). Genetic diversity analysis in brassica varieties through RAPD markers. Bangladesh Journal of Agricultural Research. 34 (3): 493-503.
Gupta P.K., Varshney, R.K., and Sharma, P.C. (1999). Molecular markers and their applications in wheat breeding. Plant Breeding, 118: 369–390.
Gupta, R., Chandrashekhar, U.S., Yadav, J.B., Chakrabarty, S.K. and Dadlani, M. (2012). Assessment of genetic relatedness among Indian mustard (Brassica juncea) genotypes using morphological traits and DNA marker. The Indian Journal of Agricultural Sciences, 82(9): 746-752.
Gupta, N., Zargar, S.M., Gupta, Moni and Gupta, S.K. (2014). Assessment of Genetic Variation in Indian Mustard (Brassica juncea L.) Using PCR-Based Markers. Molecular Plant Breeding, 5(3): 10-179.
Karp, A., Kresovich, S., Bhat, K.V., Ayad, W.G. and Hodgkin, T. (1997). Molecular tools in plant genetic resources conservation: a guide to the technologies; in IPGRI Technical Bull. No. 2. International Plant Genetic Resources Institute, Rome, Italy.
Khan, W.M., Munir, I., Arif, F.M., Iqbal, A., Ali, I., Ahmad, D., Ahmad, M., Mian, A., Bakht, J. and Swati, Z.A. (2011). Genetic diversity analysis among Brassica juncea germplasm using RAPD markers. Africa Journal of Biotechnology, 10: 3654-3658.
Kresovich, S., Williams, J.G.K., McFerson, J.R., Routman, E.J. and Schaal, B.A. (1992). Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theoretical and Applied Genetics, 85: 190-196.
Mailer, R.J., Scarth, R. and Fristensky, B. (1994). Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphism amplified from arbitrary primers. Theoretical and Applied Genetics, 87: 697-704.
Marshall, P., Marchand, M.C., Lisieczko, Z. and Landry, B.S. (1994). A simple method to estimate the percentage of hybridity in canola (Brassica napus) F1 hybrids. Theoretical and Applied Genetics, 89: 853–858.
Misra, A.K. (2011) Variability for agromorphological traits in germplasm of yellow sarson (Brassica rapa L. var. yellow sarson). Cruciferae Newsletter, 30: 33-34.
Murry, H.C. And Thompson, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research, 8: 4321-4325.
Quiros, C.F., Hu, J., This, P., Cherve and Delseny, M. (1991). Developmental and chromosomal localization of genomespecific markers by the polymerase chain reaction. Theoretical and Applied Genetics, 82: 627-632.
Ren, J., McFerson, J.R., Li, R., Kresovich, S. and Lamboy, W.F. (1995). Identities and relationships among Chinese vegetable Brassicas as determined by random amplified polymorphic DNA markers. Journal of American Society for Horticultural Science, 120: 548-555.
Saha, S., Molla, R., Chandra, D. and Rahman, L. (2008). Assessment of genetic variation and relationships within the varieties of four Brassica species by RAPD markers. Australian Journal of Crop Science, 2: 105-114.
Santos, J.B., Nienhuis, J., Skroch, P., Tivang, J. and Slocum, M.K. (1994). Comparison of RAPD and RFLP genetic markers in determining genetic similarity among Brassica oleracea L. genotypes. Theoretical and Applied Genetics, 87: 909-915.
Shengwu, H., Ovesna, J., Kucera, L., Kucera, V. and Vyvadilova, M. (2003). Evaluation of genetic diversity of Brassica napus germplasm from China and Europe assessed by RAPD markers. Plant Soil and Environment, 49: 106-113.
Tautz, D. (1989). Hypervariability of simple sequences as general sources for polymorphic DNA markers. Nucleic Acids Reserch, 17(16): 6463-6471.
Thormann, C.E., Ferreira, M.E., Camargo, L.E.A., Tivang, J.G. and Osborn, T.C. (1994). Comparison of RFLP and RAPD markers to estimating genetic relationships within and among Cruciferous species. Theoretical and Applied Genetics, 88: 973-980.
Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, T.V.D., Hornes, M., Frejters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. (1991). AFLP a new technique for DNA fingerprinting. Nucleic Acid Research, 23: 4407-4414.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research, 18: 6531-6535.
Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research, 18: 7213-7218.
Yassein, A.A.M., Abdal-Aziz, S.A. and Galab, K.H. (2013). Evaluation of Genetic diversity and heritability of some rapeseed (Brassica napus) genotypes using agronomical and molecular traits. Journal of Advanced Laboratory Research in Biology, 4(4): 131-134.
Yousuf, M., Bhat, T.M. and Kudesia, R. (2012). Comparative genetic diversity studies in mustard (Brassica Juncea) varieties using randomly amplified polymorphic DNA (RAPD) analysis. African Journal of Biotechnology, 12: 3430-3434.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
