Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp.

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Published Sep 1, 2016
DOI: https://doi.org/10.31018/jans.v8i3.953

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Mukesh R. Jangra
Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana), INDIA
Ritu Batra
Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana), INDIA
Ikbal Ikbal
Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana), INDIA
Akanksha Jain
Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana), INDIA
Rekha Ahlawat
Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana), INDIA
Virendra K. Sikka
Department of Molecular Biology, Biotechnology & Bioinformatics, College of Basic Sciences & Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004 (Haryana), INDIA

Abstract

Using glucose as carbon source and mustard cake and yeast extract as nitrogen sources bacterial isolate Pseudomonas B2 exhibited a maximum PHB recovery of 0.620 (in terms of O.D.) and PHB weight of 0.27g/L in 96 h. To determine the possibility of growth potential of Pseudomonas spp., it was grown in different carbon sources like fructose, glucose, maltose, mannitol etc. and it was found that glucose yielded good growth and PHB production. In order to incorporate cost effective nitrogen and carbon source, mustard cake and cotton cake as nitrogen source and molasses as carbon were used in medium. Statistical media optimization design was used to optimize the culture conditions for maximizing the PHB production. A maximum of 0.37 g/L of PHB and 0.746 (O.D.) PHB recoveries were obtained using optimized concentrations. Batch kinetics can be used for model development, which will make possible simulation of nutrient limited cultivation(s) for over accumulation of PHB. FTIR studies confirmed the presence of PHB.

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Keywords

Central composite design, Pseudomonas spp., Polyhydroxybutyrate, Response Surface Methodology

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Issue
Vol 8 No 3 (2016)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp. (2016). Journal of Applied and Natural Science, 8(3), 1278-1285. https://doi.org/10.31018/jans.v8i3.953