##plugins.themes.bootstrap3.article.main##

Mukesh R. Jangra Ritu Batra Ikbal Ikbal Akanksha Jain Rekha Ahlawat Virendra K. Sikka

Abstract

Using glucose as carbon source and mustard cake and yeast extract as nitrogen sources bacterial isolate Pseudomonas B2 exhibited a maximum PHB recovery of 0.620 (in terms of O.D.) and PHB weight of 0.27g/L in 96 h. To determine the possibility of growth potential of Pseudomonas spp., it was grown in different carbon sources like fructose, glucose, maltose, mannitol etc. and it was found that glucose yielded good growth and PHB production. In order to incorporate cost effective nitrogen and carbon source, mustard cake and cotton cake as nitrogen source and molasses as carbon were used in medium. Statistical media optimization design was used to optimize the culture conditions for maximizing the PHB production. A maximum of 0.37 g/L of PHB and 0.746 (O.D.) PHB recoveries were obtained using optimized concentrations. Batch kinetics can be used for model development, which will make possible simulation of nutrient limited cultivation(s) for over accumulation of PHB. FTIR studies confirmed the presence of PHB.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Central composite design, Pseudomonas spp., Polyhydroxybutyrate, Response Surface Methodology

References
Ahn, W,S., Park, S.J. and Lee, S.Y. (2001). Production of poly(3-hydroxybutyrate) from whey by cell recycle fedbatch culture of recombinant Escherichia coli. Biotechnol. Lett. 23:235–40.
Anderson, A.J. and Dawes, E.A. (1990). Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54:450–72.
Beringer, J.F., Beynon. I.L., Buchanan-Wollaston, A.V. and Johnston, A.W.B.(1978). Transfer of the drug-resistance transposon Tn5 to Rhizobium. Nature. 276: 633-634.
Bhuwal, A.K., Singh, G., Aggarwal, N.K., Goyal, V. and Yadav, A. (2014). Poly-?-hydroxybutyrate production and management of cardboard industry effluent by new Bacillus sp. NA10. Bioresour. Bioprocess. 1:2-11.
Byrom, D. (1887). Polymer synthesis by microorganisms: technology and economics. Trends Biotechnol. 5:246–50.
Choi, J.Y., Lee, J.K., You, Y., Park, W.H. (2003). Epoxidized polybutadiene as a thermal stabilizer for poly(3-hydroxybutyrate). II. Thermal stabilization of poly (3-hydroxybutyrate) by epoxidized polybutadiene. Fiber Polym 4:195–198.
Jangra, M. and Sikka, V.K. (2015). Expeditious and ecofriendly production of poly-?-hydroxy butyrate in bacteria grown on renewable agribyproducts. Int. J. Recent Sci. Res. 6:6400-6404.
Jangra, M.R., Jain, A., Batra, R., Ahlawat, R. and Sikka, V.K. (2016). Stastical analysis for optimization of bacterial polyhydroxybutyrate production using agri byproducts. Indian J. Eco. 43:557-562.
Labuzeck, S. and Radecka, I. (2001). Biosynthesis of tercopolymer by Bacillus cereus UW85. J. Appl. Microbiol. 90:353–357.
Launen, L.A., Pinto, L.J. and Moore, M.M. (1999). Optimization of pyrene oxidation by Penicillium janthinellum using response surface methodology. Appl. Microbiol. Biotechnol. 51:510–5.
Lee, S.Y. (1996). Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng. 49:1–14.
Montgomery, D.C. (2005). Design and analysis of experiments, 6th edn. Wiley, New York.
Muthazhagan, K. and Thangaraj, M. (2014). Production and ftir analysis of bio-polymer by bacillus sp isolated from vellar estuary sediment. Int. J. Sci. Invention Day. 3:625-638.
Sharma P and Bajaj BK (2015). Cost-effective-substrates for production of poly-?-hydroxybutyrate by a newly isolated Bacillus cereus PS-10. J. Environ. Biol. 36:1297-304.
Slepecky, R.A. and Law, J.H. (1961). Assay of poly-?-hydroxybutyric acid. J. Bacteriol. 82: 33-36.
Veeramanikandan, V., Mrudula, S., Yuvaraj, R. and Balaji, P. (2013). Optimization of Bacterial Poly – ? – Hydroxy Butyrate (PHB) Production from Different Industrial Waste using Central Composite Design. Int. J. Com. Appl. 62:27-34.
Wei, Y.H., Chen, W.C., Wu, H.S. and Janarthanan, O.M. (2011). Biodegradable and Biocompatible Biomaterial, Polyhydroxybutyrate, Produced by an Indigenous Vibrio sp. BM-1 Isolated from Marine Environment. Mar. Drugs. 9: 615-624.
Citation Format
How to Cite
Jangra, M. R., Batra, R., Ikbal, I., Jain, A., Ahlawat, R., & Sikka, V. K. (2016). Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp. Journal of Applied and Natural Science, 8(3), 1278–1285. https://doi.org/10.31018/jans.v8i3.953
More Citation Formats:
Section
Research Articles