Isolation, purification, and characterization of xylanase produced by three species of bacillus under submerged fermentation conditions
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Abstract
This study focuses on the screening and identification of bacteria, which can produce alkaline xylanase at alkaline pH and high temperature. Bacterial isolates from enriched decaying soil, capable of hydrolyzing xylan were screened. Selected and purified 13 bacterial colonies (Bacilli and Kurthia) grown on xylan- nutrient agar slants, were activated and transferred into the fermentation medium. Three highest xylanase producing isolates (Bacillus badius, Kurthia gibsonii, Bacillus circulans) were selected for further studies and the xylanase produced by them were screened for their kinetic properties. The optimum temperature for the activity of the xylanase from Isolates A was 50oC; and for Isolate B was 40oC, while that of Isolate C was 30oC. The optimum pH value for the xylanase from isolate A and B was 9.0. In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp bio-bleaching processes but also in the nutraceutical industry.
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Bacillus badius, Kurthia gibsonii, Submerged fermentation, Xylanase
Archana, A. and Satyanarayana, T. (1997). Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation. Enzyme and Microbial Tech. 21 (1) : 12–17.
Azeri, C., Tamer A.U. and Oskay M. (2010).Thermoactive cellulase-free xylanase production from alkaliphilic Bacillus strains using various agro-residues and their potential in biobleaching of kraft pulp. Afr. J. Biotech. 9 (1) : 63-72.
Bergey, D.H., Holt, J.G., Krieg, N.R. and Sneath, P.H.A. (1994). Bergey's Manual of Determinative Bacteriology (9th ed.).
Chidi, S.B., Godana, B., Ncube, I., Van Rensburg, E.J., Cronshaw, A. and Abotsi, E.K. (2008). Production, purification and characterization of cellulase-free xylanase from Aspergillus terreus UL 4209. Afr. J. Biotech. 7(21) : 3939–3948.
Flores, M.E., P´erez, R. and Huitr, C. (1997). ?-Xylosidase and xylanase characterization and production by streptomyces sp. CH-M-1035. Letters in Appl. Microbio. 24 (5) : 410–416.
Gupta, S., Bhushan, B., Hoondal G.S. and Kuhad, R.C. (2001). Improved xylanase production from a haloalkalophilic Staphylococcus sp SG-13 using inexpensive agricultural residues. World J Microbio. and Biotech. 17 : 5-8.
Gupta, U. and Kar, R. (2008). Optimization and scale-up of cellulase free endo xylanase production by solid state fermentation on corncob and by immobilized cells of a thermotolerant bacterial isolate. Jordan J Bio. Sci., 1: 129-134.
Kalia, V.C., Raizada, N. and Sonakya, V. (2000). Bioplastics. J Sci. and Indust. Res. 59:433–445.
Kamble, R.D. and Jadhav, A.R. (2012). Isolation, purification, and characterization of xylanase produced by a new species of Bacillus in solid state fermentation. Int. J Microbio., Article ID 683193, pp. 8.
Kang, M.K., Maeng P.J. and Rhee, Y.H. (1996). Purification and characterization of two xylanases from alkaliphilic Cephalosporium sp. strain RYM-202. Appl. and Environ. Microbio. 62 (9) : 3480–3482.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measured with the Folin phenol reagent. J Bio. Chem., 193: 265-275.
MacFaddin, J.F. (2000). Biochemical tests for identification of medical bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA
Mahilrajan, S., Balakumar, S. and Arasaratnam, V. (2012). Screening and identification of a thermophilic and alkalophilic bacterium producing xylanase. Adv. Appl. Sci. Res. 3 (1) : 242-250.
Miller G.L. (1959). Use of dinitrosalicylic acid reagent for the determination of reducing sugars. Ana. Chem., 31 : 538-542.
Prakash, S., Veeranagouda, Y., Kyoung, I. and Sreeramulu, K. (2009). Xylanase production using inexpensive agricultural wastes and its partial characterization from a halophilic Chromohalobacter sp TPSV101. World J Micro. Biotech. 25 : 197-204.
Sneath, P.H.A. (1986). Endospore-forming gram-positive rods and cocci, Bergey’s manual of systematic bacteriology. The William and Wilkins Co., Baltimore, 1104-1139.
Srinivasan, M.C. and Rele, M.V. (1999). Microbial xylanases for paper industry. Cur. Sci., 77 (1) : 137–142.
Sudan R. and Bajaj, B.K. (2007). Production and Biochemical characterization of xylanases from an alkalitolerant novel sp Aspergillus niveus RS2. World J Micro. and Biotech. 23(4): 491-500.
Viikari, V., Kantelinen, J. and Linko, M. (1994). Xylanases in bleaching: from an idea to the industry FEMS. Microbio. Rev. 13 : 335-350.
Viikari, L., Kantelinen, A., Poutanen, K. and Ranua, M. (1990). Characterization of pulps treated with hemicellulolytic enzymes prior to bleaching in Biotechnology in Pulp and Paper Manufacture, T. K. Kirk and H. M. Chang, Eds.,145–151, Butterworth-Heinemann, Stoneham, Mass, USA.
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