Evaluation of management of bacterial stalk rot of maize (Dickeya zeae) using some chemicals and bio-agents
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Abstract
A virulent strain (M-13) of Dickeya zeae causing bacterial stalk rot of maize, isolated from Hoshiarpur district of Punjab was tested against different antimicrobial agents. Five antibacterial chemicals viz., stable bleaching powder, streptocycline, cristocycline, blitox, kocide and one bioagent (Pseudomonas fluorescens) were evaluated against D. zeae under in vitro and in vivo condition. The chemicals were tested with different concentrations i.e. 50, 100, 200, 500, and 1000 and >1000 ppm and bioagent supernatant used as such without any dilutions. All the test chemicals and bioagent (P. fluorescens strains) reduced the log cfu/ml of D. zeae. Amongst chemicals, stable bleaching powder with 100 ppm concentration showed most effective antibacterial activity which reduced the population of D. zeae with 6.35 log cfu/ml and amongst P. fluorescence strains, MPF-5 strain showed effective result with 8.07 log cfu/ml. In field condition bleaching powder also showed good result to reduce the disease severity with increased the crop yield when used as both methods (drenching and dusting). However, post inoculation drenching with 100ppm concentration showed minimum mean of disease severity (7.13%) with high yield (7.4 kg) in all three maize cultivars (Dekalb Double, Punjab Sweet Corn-1 and PMH-1). The increase yield per cent as compared to control was 52.4% in Dekalb Double, 64% in Punjab Sweet Corn-1 and 57.9 % in PMH-1 maize cultivars.
Article Details
Article Details
Bacterial stalk rot, Cultivars, Dickeya zeae, Maize management
Adaskaveg, J. E., Förster, H., and Wade, M. L. (2011). Effectiveness of kasugamycin against Erwinia amylovora and its potential use for managing fire blight of pear. Plant Dis. 95: 448-454.
Chakravarti, B.P. and Rangarajan, M. (1966). Streptocycline, an effective antibiotic against bacterial Plant pathogens. Hindustan Antibiot. Bull. 8: 209-211.
Ghosh, P. P. and Mandal, N. C. (2009). Some disease management practices for bacterial wilt of potato. The Journal of Plant Protection Sciences 1 : 51-54, 2009.
Jones, A. L., and Schnabel, E. L. (2000). The development of streptomycin resistant strains of E. amylovora. In: Fire Blight: The disease and its causative agent, E. amylovora. J. L. Vanneste, ed. CABI Publishing, New York, pp 335-351.
Karkouri, A.E., Hassani, F.Z. E., Mzibri, M.E., Benlemlih, M.E. and Hassouni, M. (2010). Isolation and Identification of an Actinomycete Strain with a Biocontrol Effect on the Phytopathogenic Erwinia chrysanthemi 3937VIII Responsible for Soft Rot Disease. Ann. Microbiol. 60: 263-68.
Kumar, A., Hunjan, M.S., Singh, P.P. and Kaur H. (2015) Status of bacterial stalk rot of maize in Punjab. Plant. Dis. Res. 30: 97-99.
Lal S (1981) Bacterial stalk rot of maize. In:Techniques of scoring for resistance to important diseases of maize. All India co-ordinated maize improvement project, IARI, New Dehli, pp 82-86.
Lal, S., Thind, B.S., Payak, M. and Ranfro, B.L. (1970). Bacterial stalk rot of maize: Resistant breeding and chemical control. Indian Phytopath. 23:150-7.
Nagaraj, M.S., Umashankar, N., Palanna, K.B. and Khan, A.N.A. (2012). Etiology and management of tip-over disease of banana by using biological agents. Int. J. Adv. Biotechnol. Res. 2: 483-86.
Parashar, R.D., Sindhan, G. S. and Singh J. P. (1986). Field application of Kloricin for the control of soft rot of potato in field and storage. J. Indian Potato Assoc. 13: 81-82.
Raghuwanshi, K.S., Hujare, B. A., Chimote, V.P. and Borkar, S.G. (2013). Characterization of xanthomonas axonopodis pv. punicae isolates from Western Maharashtra and their sensitivity to chemical treatments. The Bioscan 8: 845-850.
Randhawa, P.S. (1977). Control of bacterial stalk rots and stripe disease of Maize (Zea mays L.). M Sc thesis submitted to Punjab Agricultural University Ludhiana.
Rangarajan, M. and Chakravarti, B. P. (1967). A strain of Pseudomonas lapsa isolated from corn seed causing stalk rot in India. Plant. Dis. Rep. 51:764-766.
Rangarajan, M. and Chakravarti, B.P. (1969). Efficacy of antibiotics and fungicides against corn stalk rot bacteria. Hindustan Antibiot. Bull. 2:177-79.
Samson, R., Legendre, J.B., Christen, R., Fischer, M., Achouak, W. and Gardan, L. (2005). Transfer of Pectobacterium chrysanthemi (Burkholder et al 1953) Brenner et al 1973 and Brenneria paradisiaca to the genus Dickeya gen. nov. as Dickeya chrysanthemi comb. nov. and Dickeya paradisiaca comb. nov. and delineation of four novel species, Dickeya dadantii sp. nov., Dickeya dianthicola sp. nov., Dickeya dieffenbachiae sp. nov. and Dickeya zeae sp. nov. Int. J. Sys. Evol. Microbiol. 55:1415-27.
Sharma, J. P., Kumar S. (2000) Management of Ralstonia wilt through soil disinfectant, mulch lime and cakes in tomato (Lycopersicon esculentum). The Indian Journal of Agricultural Sciences 70.17-19.
Sharma, J.P. and Kumar, S. (2009). Linear reduction of propagules of Ralstonia solanacearum in soil by cake and chemicals. Indian Phytopath. 62: 49-53.
Shekhawat, G.S., Bhal, B.K., Kishore, V., Sangar, R.B.S., Patel, R.L., De, B.K., Sinha, S.K. and Pani, A.K. (1990). Control of bacterial wilt of potatoes by agronomic practices. J. Indian Potato. Assoc. 17: 52-60.
Thind, B.S. and Payak, M.M. (1972). Antibiotics and bleaching powder in the control of bacterial stalk rot of maize. Hindustan Antibiot. Bull. 15: 73-74.
Thind, B.S. and Payak, M.M. (1978). Evaluation of maize germplasm and estimation of losses to Erwinia stalk rot. Pl. Dis. Rep. 62: 319-23.
Yamasaki, M., Kusakari, S., Narita, K., Osamura, K. and Nagai, M. (2006). Control of root rot disease of tomatoes by using weak acidic electrolyzed water (WAEW) in the hydroponic culture solution. J. Antibac. Antifung. Agents 34:543–549.
Yuliar, Nion, Y. A. and Koki Toyota, K. (2015). Recent Trends in Control Methods for Bacterial Wilt Diseases Caused by Ralstonia solanacearum. Microbes Environ. 30:11.
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