Physiological and biochemical response to higher temperature stress in hot pepper (Capsicum annuum L.)
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Abstract
The present study was conducted to evaluate the physiological and biochemical changes in some thermotolerant and thermosensitive chilli (Capsicum annuum L.) genotypes. Fourteen chilli genotypes (SL 461, PP 404, DL 161, MS 341, VR 521, PB 405, PS 403, SD 463, FL 201, AC 102, S 343, SL 462 and SL 464 along with sensitive check [Royal Wonder of bell pepper] were evaluated for heat tolerance. The observations on morpho-physiological and biochemical parameters were recorded at 45, 65, 85 and 105 days after transplanting (DAT) (high temperature period). On the basis of our studies, genotypes S 343, AC 102 and FL 201 were found to be relatively thermotolerant. However, high temperature markedly decreased the photosynthetic activity of chilli plants by decreasing the photosynthetic pigments in leaf chloroplasts of all the genotypes. The levels of ascorbic acid, total soluble sugars and total phenols increased in the leaves of all the genotypes with the maturity of the crop. Electrolyte leakage and proline content also increased with rise in temperature. Genotypes AC 102 and S 343 were able to accumulate the maximum ascorbic acid, proline, total soluble sugars and total phenols under heat stress conditions. Decrease in fruit set percent led to reduction in the total yield per plant. Maximum yield was observed in genotype S 343 followed by FL 201.
Article Details
Article Details
Chilli, High Temperature, Photosynthetic activity, Proline
Almesalmani, M., Deshmukh, P. S. and Sairam. R. (2009) High temperature stress tolerance in wheat genotypes: role of antioxidants defence enzyme. Acta Agron Hung., 57: 1-14.
Anderson, J. M. and Boardman, N. K. (1964). Studies on greening of dark brown bean plants: development of phytochemical activity. Aus J Bio., 17: 93-101.
Anonymous. (2012). Package of practices for vegetable crops, Punjab Agricultural University, Ludhiana, Punjab. PP
AVRDC (2003) Development of high yielding, diseaseresistant chilli peppers. In: AVRDC Progress report - 2003. Asian Vegetable Research and Development Centre, Shanhua, Taiwan. pp. 41-46.
Bates, L. S. Waldeen, R. P. O. and Teare, I. D. (1973). Rapid determination of free proline in water stress studies. Plant Soil., 39: 205-07.
Camejo, D. and Torres, W. (2001). High temperature effects on tomato (Lycopericon esculetinum) pigment and protein content and cellular viability. Cultivos Trop., 22: 13-17.
Challinor, A., Wheeler, T. Craufurd, P. Ferro, C. and Stephenson, D. (2007). Adaptation of crops to climate change through genotypic response to mean and extreme temperatures. Agric Ecosyst Environ., 119-190.
Conforti, F., Statti, G. A., and Menichini, F. (2007). Chemical and biological variability of hot pepper fruit (Capsicum annuum var. acuminatum) in relation to maturity stage. Food Chemis., 102: 1096-1104.
Cornic, G. (2000). Drought stress inhibits photosynthesis by decreased stomatal aperture not by affecting ATP synthesis. Trends Pl Sci., 5: 187-88.
Dahal, K. C., Sharma, M. D., Dhakal, D. D., and Shakya, S. M., (2006). Evaluation of heat tolerant chilli (Capsicum annuum L.) genotypes in western terai of Nepal. J Inst Agric Anim Sci., 27: 59-64.
Dubois, M., Gilles, K. A., Hamilton, J. K., Roberts, P. A., and Smith, F., (1956). Colorimetric method for the determination of sugars and related substances. Anal Chem., 28: 352-56.
Fletcher, R. A. and Drexlure, D. M. (1980). Interactions of dichloromethyl and 2, 4-D in cultivated oats (Avena sativa). Weed Sci., 28: 363-66.
Gulen, H. and Eris, A. (2004) Effect of heat stress on peroxidase activity and total protein content in strawberry plants. Plant Sci., 166: 739-44.
Kaya, H., Shibahara, K., Taoka, K., Iwabuchi, M., Stillman, B. and Araki, T. (2001). FASCIATA genes for chromatin assembly factor-1 in Arabdopsis maintain the cellular organization of apical meristems. Cell., 104-131.
Komayama, K., Khatoon, M., Takenaka, D., Horie, J., Yamashita, A., Yoshioka, M., Nakayama, Y., Yoshida, M., Ohira, S., Morita, N., Velitchkova, M., Enami, I. and Yamamoto, Y. (2007). Quality control of photosystem II: cleavage and aggregation of heat-damaged D1 protein in spinach thylakoids. Biochem Biophys Acta., 1767: 838-46.
Kumar, S., Kaur, R., Kaur, N., Bhandhari, K., Kaushal, N., Gupta, K., Bains, T. S. and Nayyar, H. (2011). Heat stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus) is partly mitigated by ascorbic acid application and related to reduction in oxidative stress. Acta Physiol Plant., 33: 2091-2101.
Prasad, P., Pisipati, S., Mutava, R. and Tuinstra, M. 2008. Sensitivity of grain sorgum to high temperature stress during reproductive development. Crop Sci., 48: 1911-17.
Qin, A. G., Gao, J. J. and Yu, X, C. (2009). Effects of high - and low temperature stresses on ascorbic acid metabolism system in potato leaves. J Appl Eco., 20(12): 2964-70.
Rivero, R. M., Ruiz, J. M., Garcia, P. C., Lopez Lefebre, L. R., Sanchez, E. and Romero, L. (2001). Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Sci., 160: 315-31.
Roe, J. H. and Oesterling, M. J. 1943. The determination of dehydroascorbic acid and ascorbic acid in plant tissue by the 2, 4 dinitrophenyl hydrazine method. J Biol Chem., 152: 511-17.
Saha, S. R, Hossain, M. M., Rahman, M. M.,Kuo, C. G. and Abdullah, S. (2010). Effect of high temperature stress on the performance of twelve sweet pepper genotypes. J Agril Res., 35(3): 525-34.
Scafaro, A. P., Haynes, P. A. and Atwell, B. J. (2010). Physiological and molecular changes in Oryza meridionalis Ng. a heat tolerant spices of wild rice. J Exp Bot., 61: 191-202.
Sohan, S. O. and Back, K. (2007) transgenic rice tolerant to high temperature with elevated contents of dienoic fatty acids. Biol plant 51: 340-42.
Sung D Y, Kaplan F, Lee K J and Guy C L (2003) Acquired tolerance to temperature extremes. Trends Plant Sci., 8: 179–87.
Swain, T. and Hillis, W. E. 1959. The phenolic constituents of Prunus domestica-The qualitative analysis of phenolic constituents. J Sci Fd Agric., 10: 63-68.
Wahid, A., Gelani, S., Ashraf, M., and Foolad, M. R. (2007). Heat tolerance in plants: An overview. Environ Exp Bot., 61: 199-223.
Wani, S. H., Singh, N. B.,Haribhushan, A. and Mir, J. I. (2013). Compatible solute engineering in plants for abiotic stress tolerance-role of glycine betain. Curr Genomics., 14(3) 157–65.
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