Biochemical investigations on vigour enhancement in aged seeds upon seed priming in onion

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Published Jun 1, 2016
DOI: https://doi.org/10.31018/jans.v8i2.885

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Umesha Umesha
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur -584102 (Karnataka), INDIA
S.N. Vasudevan
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur -584102 (Karnataka), INDIA
K. Bhanuprakash
Division of Seed Science and Technology, Indian Institution of Horticultural Research, Hesaraghatta, Bangalore- 560089 (Karnataka), INDIA
B. Manjunatha
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur -584102 (Karnataka), INDIA
G. Sarika
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore 560065 (Karnataka), INDIA
N. Amruta
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore 560065 (Karnataka), INDIA

Abstract

Loss in seed quality that occurs during all process, from maturation in the field to storage, leads to seed deterioration. Storage of onion seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzymes and protein levels. This deterioration can be rectified to the extent possible by the technique of seed priming. The results clearly indicated that priming will restore the lost seed vigour in aged seeds due to reactivation of enzyme activity in old seeds. The germination percentage improved from 60% to 79.5% and 72.5% in GA3 and hydroprimed, respectively. Priming will also restore the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds.

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Keywords

Esterase, Onion, Peroxidase, Sodium Dodecyl Sulfate-Polyacrylamide gel electrophoresis SDS-PAGE, Vigour

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Issue
Vol 8 No 2 (2016)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Biochemical investigations on vigour enhancement in aged seeds upon seed priming in onion. (2016). Journal of Applied and Natural Science, 8(2), 855-859. https://doi.org/10.31018/jans.v8i2.885