Biochemical investigations on vigour enhancement in aged seeds upon seed priming in onion

##plugins.themes.bootstrap3.article.sidebar##

PDF
Published Jun 1, 2016
DOI: https://doi.org/10.31018/jans.v8i2.885

##plugins.themes.bootstrap3.article.main##

Umesha Umesha
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur -584102 (Karnataka), INDIA
S.N. Vasudevan
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur -584102 (Karnataka), INDIA
K. Bhanuprakash
Division of Seed Science and Technology, Indian Institution of Horticultural Research, Hesaraghatta, Bangalore- 560089 (Karnataka), INDIA
B. Manjunatha
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, Raichur -584102 (Karnataka), INDIA
G. Sarika
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore 560065 (Karnataka), INDIA
N. Amruta
Department of Seed Science and Technology, College of Agriculture, University of Agricultural Sciences, GKVK, Bangalore 560065 (Karnataka), INDIA

Abstract

Loss in seed quality that occurs during all process, from maturation in the field to storage, leads to seed deterioration. Storage of onion seeds under ambient, hot and humid conditions is very problematic since these conditions deteriorate seed quality faster. Seed deterioration is associated with many metabolic defects that occur due to changes in enzymes and protein levels. This deterioration can be rectified to the extent possible by the technique of seed priming. The results clearly indicated that priming will restore the lost seed vigour in aged seeds due to reactivation of enzyme activity in old seeds. The germination percentage improved from 60% to 79.5% and 72.5% in GA3 and hydroprimed, respectively. Priming will also restore the lost seed vigour in aged seeds due to reactivation of proteins in old seeds and expression of these proteins in priming treatments are related to priming induced proteins in contrast to their absence in the aged seeds which are necessary for germination and longevity of seeds.

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Esterase, Onion, Peroxidase, Sodium Dodecyl Sulfate-Polyacrylamide gel electrophoresis SDS-PAGE, Vigour

References
Basu, R.N. (1976). Physico-chemical control of see deterioration. Seed Research, 4:15–23.
Bewley, J.D., (1997). Seed germination and dormancy. Plant Cell, 9: 1055-1066.
Bhanuprakash, K., Yogeesha, H.S. and Arun, M.N. (2010). Physiological and biochemical changes in relation to seed quality in ageing Bell pepper. Indian J. of Agric. Sci., 80 (9): 9-11.
Dure, L.II., Crouch, M., Harada, J., Mundy, H.J., Quatrano, R., Thomas, T. and Sung, Z.R. (1989). Common amino-acid sequence domains among the LEA proteins of higher plants. Plant Molecular Biology, 12:475- 486.
Fujikora, Y. and Karssen, C.M. (1992). Effects of controlled deterioration and osmopriming on protein synthesis of cauliflower seeds during early germination. Seed Sci. Res., 2: 23-31.
Glaszman, J.C. (1987). Isoenzymes and classification of Asian rice varities. Theor. Appl. Genet., 74: 21-30.
Gurusinghe, S., Powell, A.L.T. and Bradford, K.J. (2002). Enhanced expression of Bip is associated with treatments that extend storage longevity of primed tomato seeds. J. Amer. Soc. Hort. Sci., 127: 528-534.
Haroun S.A. and Hussein, M.H. (2003). The promo tive effect of algal biofertilizers on growth, protein pattern and some metabolic activities of Lupinus termis plants grown in siliceous soil. Asian J. Plant Sci., 2: 944-951.
Heydecker, W., Higgins, J. and Gulliver, R.L. (1973). Ac-celerated germination by osmotic seed treatment. Na-ture, 246: 42-44.
Job, C., Kersulec, A., Chareyre, S., Pepin, R. And Job, D., (1997), The solubilization of the basic subunit of sugarbeet seed 11-S globulin during priming and early germination. Seed Sci. Res., 7: 225-243.
Laemeli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Na-ture, 227: 680–685
Laemeli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Na-ture, 227: 680–685
McDonald, M.B. (1999). Seed deterioration: physiology, repair and assessment. Seed Sci. Technol., 27: 177–237.
Muhammad, A. and Muhammad, A.A. (2002). Evaluation of physiological quality of onion seed stored for different periods. International J. Agric. Boil., 4 (3): 365-369.
Powell, A.A., Yule, L.J., Jing, H.C., Groot, S.P.C., Bino, R.J. and Pritchard, H.W. (2000). The influence of aer-ated hydration seed treatment on seed longevity as as-sessed by the viability equations. J. Exptl. Bot., 51(353): 2031-2043.
Sathish, S. Sundareswaran, S., Senthil, N. and Ganesan, K.N. (2012). Biochemical changes due to seed priming in maize hybrid COH(M) 5. Res. J. of Seed Sci., 5 (3): 71-83.
Soumya, K.J. (2011). Studies on physiological, biochemical and molecular aspects of seed invigouration in cucum-ber (Cucumis sativus L.) Ph.D. Thesis, Univ. Agric. Sci., Bengaluru, Karnataka (India).
Varier, A., Alice, K.V. and Dadlani, M. (2010). Subcellualr basis of seed priming. Current Sci., 99 (4): 450-456.
Soumya, K.J. (2011). Studies on physiological, biochemical and molecular aspects of seed invigouration in cucum-ber (Cucumis sativus L.) Ph.D. Thesis, Univ. Agric. Sci., Bengaluru, Karnataka (India).
Varier, A., Alice, K.V. and Dadlani, M. (2010). Subcellualr basis of seed priming. Current Sci., 99 (4): 450-456.
Issue
Vol 8 No 2 (2016)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Biochemical investigations on vigour enhancement in aged seeds upon seed priming in onion. (2016). Journal of Applied and Natural Science, 8(2), 855-859. https://doi.org/10.31018/jans.v8i2.885