The study was carried out to standardize the optimal growth, sporulation and production of perfect stage of pathogen on different media. Among different media used such as Potato dextrose Agar (PDA), Oat meal Agar, Ragi flour agar, yeast extract + 2% soluble starch, Host extract + 2% soluble sucrose agar, Potato dextrose agar + Biotin + Thiamine and Rice flour agar, Oat meal agar and potato dextrose agar was found to be best media for radial growth and sporulation of M. grisea. Maximum conidia length (9.46Î¼m) and breadth (7.36Î¼m) was recorded in Oat meal agar followed by Potato dextrose agar and least conidia length (6.15 Î¼m) and breadth (5.11 Î¼m) was recorded in ragi flour media after 20 days of inoculation. Conidial size varied in leaf and neck blast isolates, the maximum mean colony diameter of 88.00mm and 89.16mm in neck and leaf blast was recorded in Oat meal agar respectively. The maximum sporulation mean index was observed in Oat Meal agar of 3.15 Î¼m in leaf and 3.20 Î¼m in neck blast was recorded. The best growth of the pathogen was recorded at optimum pH range from 6.0 - 7.0 and temperature of 27oC. Therefore oat meal agar media was found to be best among all the media used for growth, sporulation, conidial size and colony characters of M. grisea.
Blast, Fungus, Isolates, Resistance, Rice
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