Partial characterization of toxins associated with stem end rot of mango caused by Lasiodiplodia theobromae
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Abstract
In this study, the toxicity of liquid culture media from different isolates of Lasiodiplodia theobromae was characterized and some properties of the toxic metabolites were distinguished. In this work toxin producing ability of L. theobromae was revealed by studying the physical parameters viz., osmotic potential, toxin concentration, pH, temperature and biological parameter like host specificity and wilting index. The obtained results showed that the optimal toxin-production conditions for L. theobromae in potato dextrose broth under pH 6.0, at 25-35°C for 7 days. The liquid culture from all isolates were toxic to mango plants and induced the rapid wilting. The toxin obtained from the liquid culture has thermal, acid base stability and a broad range of toxicity to main host and non-host plants. Moreover, the direct bioassay for two components of the liquid filtrates precipitated by ethanol showed that the active ingredient of the toxin is a kind of non protein substance, which was further endorsed by the papain hydrolysis analysis. Our results confirmed the chemical nature of toxic compound elucidating the favorable environmental conditions for toxin production of L. theobromae and proved potential role of toxic metabolites in the mechanism of disease development.
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Article Details
Bioassay, Lasiodiplodia theobromae, Partial characterization, Phytotoxin and Stem end rot
Bhathagar, A. and Kalpana, S, (1995). Effect of certain car-bohydrates on the pathogenesis of Colletotrichum cap-sici causing anthranose of chillies. Ind. Phytopathol., 24: 658-666.
Boonyapranai, K.B., Tungpradit, R., Lhieochaiphant, S. and Phutrakul, S. (2008). Optimization of submerged culture for the production of naphthoquinones pigment by Fusa-rium verticillioides. Chiang Mai J. Sci., 35 (3): 457-466.
Das gupta, S.N. and Zachariah, A.T. (1945). Dieback of mango. A new disease in India. Ind. J. Bot. Sci., 24 (1): 101-108.
Girish, K., Shnakara, B.S. and Raveesha, K.A. (2009). Crude toxin extract from culture filtrate of Phomopsis azadirachtae infecting neem and its phytotoxicity. Int. J. Integ. Biol., 6 (2): 79-84.
He, G., Matsuura, H. and Yoshihara, T. (2004). Isolation of ?-methylene-?-butyrolactone derivative, a toxin from the plant pathogen Lasiodiplodia theobromae. Phytochem., 65: 2803-2807.
Jayasankar, S., Litz, R.E., Gray, S. and Moon, P.A. (1999). Response of embryogenic mango cultures and seedling bioassays to a partially purified phytotoxin produced by a mango leaf isolate of Colletotrichum gloeosporioides Penz. In vitro Cell Dev. Biol. Plants., 35: 475-479.
Jeong, Y., Kim, J., Kim, S., Kang, Y., Nagamtsu, T. and Hwang, I. (2003). Taxoflovin produced by Burkholderia glumae causing rice grain rot responsible for inducing bacterial wilt in many filed crops. Plant Disease, 87: 890-895.
Johnson, G.I., Mead, A.J., Cooke, A.W. and Dean, J.R. (1992). Mango stem end rot pathogens – fruit infection by endophytic colonization of the inflorescence and pedicel. Ann. Appl. Biol., 120: 225-234.
Latha, P., Prakasam, V., Jonathan, E.I., Samiyappan, R. and Natarajan, C. (2012). Effect of culture media and envi-ronmental factors on mycelial growth and pycnidial production of Lasiodiplodia theobromae in physic nut (Jatropha curcus). J. Envi. Biol., 34: 683-687.
Litzenberger, S.C. (1949). Nature and susceptibility to Helminthosporium victoriae and resistance to Puccinia coronata in oats. Phytopathol., 39: 300-318.
Mario, A., Alves, D.C., Janaina, A.T., Raphael, C.I., Roney, R.B., Patricia, T.M., Avelina, A.I.F., Zuleica, B.F., Robert, F.H.P., Neelum, K. and Aneli, M.B. (2012). Lasiodiplodan, an exocellular (1-6)-b-D-glucan from Lasiodiplodia theobromae MMPI: production on glu-cose, fermentation kinetics, rheology and anti-proliferative activity. J. Ind. Microb. Bio., 12: 1112-1127.
Mathan, S., Subramanian, V. and Nagamony, S. (2013). Optimization and antimicrobial metabolite production from endophytic fungi Aspergillus terreus KC 582297. Euro. J. Exp. Biol., 3 (4): 138-144.
Merlin, J.N., Nimal, I.V.S., Christhuda, S., Praveen, K.P. and Agastian, P. (2013). Optimization of growth and bioac-tive metabolite production: Fusarium solani. Asian J. Pharm. Clinical Res., 6 (3): 98-103.
Naik, K.S., Hiremath, P.C. and Hedge, R.K. (1991). Toxic metabolite production by Colletotrichum gloeo-sporioides causing blight of coriander. Karnataka J. Agri. Sci., 4: 27-31.
Narasimhudu, Y. and Reddy, P.S.N. (1992). A note on gum-mosis of mango. Ind. Phytopathol., 45 (2): 261-262.
Parthasarathy, S., Nagendran, K., Narayanan, P., Rajalak-shmi, J., Thiribhuvanamala, G. and Prabakar, K. (2015). Novel insights into the phytotoxins production of Colle-totrichum gloeosporioides causing anthracnose of mango. Trends Biosci., 8 (15): 3924-3927.
Phipps, P.M. and Porter, D.M. (1998). Collar rot of peanut caused by Lasiodiplodia theobromae. Plant Dis., 82: 1205-1209.
Sharma, M.C. and Sharma, B.C. (1966). Toxic metabolite production by Colletotrichum causing citrus die-back in India. Ind. Phytopathol., 22: 67-74.
Sivanesan, A. (1984). The bitunicate ascomycetes and their ana-morphs. Cramer, Vaduz, 701pp.
Sutton, B.C. (1980). The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew, Surrey, U.K, 696pp.
Venkadaravannapa, V., Nargand, N.B., and Laxminara-yanareddy, C.N. (2007). Variation in the toxic second-dary metabolite production by Colletotrichum gloeo-sporioides isolates causing mango anthracnose. J. Plant Prot. Envi., 4 (2): 34-40.
Tovar-pedraza, J.M., Mora-aguilera, J.A., Nava-diaz, C., Teliz-ortiz, D., Valdovinos-ponce, G., Villegas-monter, A. and Hernandz-morales, J. (2012). Identification, pathogenicity, and histopathology of Lasiodiplodia theobromae on mamey sapote grafts in Guerrero, Mex-ico. Agrocien., 46: 147-161.
Zambettakis, E.C. (1954). Recherrches sur la sytematique des “Sphaeropsidales -Phaeodidymae”. Bull. Trimes. Soc. Mycol. Front., 70: 219-349.
Zhang, G.M., Fang, B.H., Chen, H. and Li, X.L. (2012). Characterisitcs of the toxin extracted from the liquid culture of Colletotrichum capsici f. nicotianae. App. Biochem. Biotech., 167 (52-61).
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