In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5Â±1.44%) in 11.3Â±0.33 days and (74.0 Â± 2.13 %) in 10.0 Â± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.
Growth regulators, In vitro, Kufri Frysona, Micro-propagation, Murashige and Skoog media
Badawi, M.A., El-Sayed, S.F., Edriss, N.H. and El-Barkouki, T.M. (1996). Factors affecting production of potato plantlets from nodal cutting. Egyptian Journal of Horti-culture, 22:117-125.
Badoni, A. and Chauhan, J.S. (2009). Effect of Growth Regulators on meristem-tip development and in vitro multiplication of Potato cultivar .Kufri Himalini.. Nature and Science, 7(9):31-34.
Badoni, A. and Chauhan, J.S. (2010). In vitro Sterilization Protocol for Micropropagation of Solanum tuberosum cv. .Kufri Himalini.. Academia Arena, 2(4).
Bhuiyan, F.R. (2013). In vitro Meristem Culture and Regeneration of Three Potato Varieties of Bangladesh. Research in Biotechnology, 4(3): 29-37, 2013.
Biniam and Tadesse, (2008). A survey of viral status on po-tatoes grown in Eritrea and in vitro virus elimination of a local variety .Tsaeda embaba.. African Journal of Biotechnology, 7(4):397-403.
Gami, R.A., Parmar, S.K., Patel, P.T., Tank, C.J., Chauhan, R.M., Bhadauria, H.S. and Solanki, S.D. (2013). Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. badshah. African Journal of Biotechnology, 12(38):5640-5647.
Hoque, M.E. (2010). In-vitro regeneration potentiality of potato under different hormonal combination. World Journal of Agricultural Sciences, 6(6):660-663.
Khurana, S.M.P., Thind, T.S. and Mohan, C. (2001). Diseases of Potato and Their Management. In: Diseases of Fruits and Vegetables and Their Management, Thind, T.S. (ed) Kalyani Publishers, Ludhiana, India.
Moeini, M.J., Armin, M., Asgharipour M.R. (2011). Effects of Different Plant Growth Regulators and Potting Mixes on Micro-propagation and Mini-tuberization of Potato. Plantlets Advances in Environmental Biology, 5(4):631-638.
Struik, R.C. and Lommen, W.J. (1991). Production, storage, and use of micro and mini tubers. Proc.11th Triennial Conf. of EARR, Edinburgh, pp.122-133.
Shibli, R.A., Abu-Ein, A.M. and Mohammed, M.A. (2001). In vitro and in vivo multiplication of virus free .Spunta. potato. Pakistan Journal of Botany, 33(1):35-41.
Sathish, S.S., Janakiraman, N., Johnson, M. (2011). .In vitro propagation of Aristolochia bracteat Retz. .A medicinally important plant. Biotechnology Research International, 2:44-52.
Vargas, E.T., De GarcÃa, E. and Oropeza, M. (2005). Somatic embryogenesis in Solanum tuberosum from cell suspension cultures: histological analysis and extracellular protein patterns. Journal of Plant Physiology, 162:449-454.
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