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Preeti Saini Madhurama Gangwar Anu Kalia Narinder Singh Deepti Narang

Abstract

Isolation of endophytic actinomycetes is an important step to screen antimicrobial compounds to curb the threat of drug-resistant strains of human pathogens. Out of the 50 endophytic actinomycetes obtained from surface sterilized root, stem and leaf tissues of Syzygium cumini, 50 isolates (30%) exhibited antimicrobial activity. Antistaphylococcal activity was displayed by most of the isolates, with maximum percent inhibition by J-10 (Mean of Inhibition Factor=12.12 mm2). A total of 8 isolates (4 each) were able to hydrolyse protein (proteinase activity) and solubilize chitin (chitinase activity). Results of thin layer chromatography confirm the production of chloramphenicol family |antibiotic by the isolate J-5. This is the first report providing an insight into untapped endophytic actinomycete milieu of Syzygium cumini yet to be explored which might be a promising source for novel antimicrobial agents.

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Keywords

Antagonism, Chitinase, Protease, SEM, Syzygium cumini, TLC

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Saini, P., Gangwar, M., Kalia, A., Singh, N., & Narang, D. (2016). Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens. Journal of Applied and Natural Science, 8(1), 416-422. https://doi.org/10.31018/jans.v8i1.809
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