Article Main

V. Ramya Udaykumar Nidoni Sharangouda Hiregoudar C. T. Ramachandra J. Ashoka V. Lavanya

Abstract

In the present study, supercritical fluid extraction (SFE) technology was applied to extract deoxynojirimycin (1-DNJ) from mulberry leaf powder using carbon dioxide (CO2) as major extraction solvent with ethanol as cosolvent, and extraction parameters such as pressure (100, 150 and 200 bar), temperature (40, 50 and 60 °C) and
dynamic extraction time (40, 60 and 80 min) were systematically investigated by full factorial design to obtain the optimum extraction efficiency and extraction yield. Under optimized conditions (pressure of 200 bar, temperature of 50 °C and dynamic extraction time of 80 min), DNJ enriched extract was obtained with high extraction efficiency (96.46 %) and extraction yield (13.41 %), enabling this product to use for nutraceutical purpose. The results indicated that SC-CO2 extraction is a promising and alternative process for recovering the bioactive compounds from mulberry leaves.

Article Details

Article Details

Keywords

Carbon dioxide, Deoxynojirimycin, Extraction efficiency, Extraction yield, Mulberry, Supercritical fluid extraction

References
Bimakr, M., Rahmana, R.A., Taipa, F.S., Chaun, L.T., Gan-jloo, A., Selamat, J. and Hamid, A. (2009). Supercriti-cal carbon dioxide (SC-CO2) extraction of bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. European Journal of Scientific Research, 33: 679-690.
Ebrahimzadeh, H., Yaminib, Y., Sefidkonc, F., Chaloosid, M. and Pourmortazavib, S. M. (2003). Chemical com-position of the essential oil and supercritical carbon dioxide extracts of Zataria multiflora Boiss. Journal of Food Chemistry, 83: 357–361.
Kim, I., Kim, J.W., Lee, H.S., Ha, N.K. and Ryu, K.S. (2001). Regional and varietal variation of 1-deoxynojirimycin (DNJ) content in the mulberry leaves. International Journal of Industrial Entomology, 2: 141-147.
Kimura, T., Nakagawa, K., Saito, Y., Yamagishi, K., Suzuki, M., Yamaki, K., Shinmoto, H. and Miyazawa, T. (2004). Determination of 1-deoxynojirimycin in mul-berry leaves using hydrophilic interaction chromatogra-phy with evaporative light scattering detection. Journal of Agricultural and Food Chemistry, 52: 1415-1418.
Lang, Q.Y. and Wai, C.M. (2001). Supercritical fluid extrac-tion in herbal and natural product studies – A practical review. Talanta, 53: 771-782.
Li, B., Xu, Y., Yu-Xia, J, Wu, Yuan-Yuan, W and Tu, Y.Y. (2010). Response surface optimization of supercritical fluid extraction of kaempferol glycosides from tea seed cake. Industrial Crops and Products, 32: 123-128.
Liza, M.S., Abdul, R.R., Mandana, B., Jinap, S., Rahmat A., Zaidul, I.S.M. and Hamid, A. (2010). Supercritical carbon dioxide extraction of bioactive flavonoid from Strobilanthes crispus (Pecah Kaca). Journal of Food and Bioproducts Processing, 88: 319-326.
Lou, D.S., Zou, F.M., Yan, H. and Gui, Z. (2011). Factors influencing the biosynthesis of 1- deoxynojirimycin in Morus alba L. African Journal of Agricultural Re-search, 6(13): 2998-3006.
Olawale, A.S. (2012). Solid-liquid extraction of oils of Afri-can elemi’s (Canarium schweinfurthii’s) fruit. Interna-tional Journal of Agricultural Engineering: CIGR, 14(2): 155-160.
Park, H.S., Lee, H.J., Shin, H.M., Lee, K. W., Lee, H., Kim, Y.S., Kim, K.O. and Kim, K.H. (2007). Effects of co-solvents on the decaffeination of green tea by supercritical carbon dioxide. Journal of Food Chemistry, 105: 1011–1017.
Tsuduki, T., Kikuchi, I., Kimura, T., Nakagawa, K. and Mi-yazawa, T. (2013). Intake of mulberry 1deoxynojirimy-cin prevents diet-induced obesity through increases in adiponectin in mice. Journal of Food Chemistry, 139: 16-23.
Vichasilp, C., Nakagawa, K., Sookwong, P., Suzuki, Y., Kimura, F., Higuchi, O. and Miyazawa, T. (2009). Opti-mization of 1-deoxynojirimycin extraction from mul-berry leaves by using response surface methodology. Journal of Bioscience Biotechnology Biochemistry, 73 (12): 2684-2689.
Wang T, Li C, Zhang H and Li J. (2014). Response surface optimized extraction of 1-deoxynojirimycin from mul-berry leaves (Morus alba L.) and preparative separation with resins. Journal of Molecules, 19: 7040-7056.
Wang, Y., Ying, L., Sun, D., Zhang, S., Zhu, Y. and Xu, P. (2011). Supercritical carbon dioxide extraction of bioac-tive compounds from ampelopsis grossedentata stems: process optimization and antioxidant activity. Interna-tional Journal of Molecular Sciences, 12: 6856-6870.
Yatsunami, K., Murata, K. and Kamei, T. (2011). 1-Deoxynojirimycin content and alfa-glucosidase inhibi-tory activity and heat stability of 1-deoxynojirimycin in silkworm powder. Journal of Food and Nutritional Sciences, 2: 87-89.
Section
Research Articles

How to Cite

Process optimization of supercritical carbon dioxide (SC-CO2) extraction parameters for extraction of deoxynojirimycin (1-DNJ) from mulberry (Morus alba L.) leaves. (2016). Journal of Applied and Natural Science, 8(1), 405-411. https://doi.org/10.31018/jans.v8i1.807