Role of vitamin C against bifenthrin induced oxidative damage in lungs of Wistar rats
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Abstract
The aim of present study was to unravel the protective role of vitamin C on oxidative stress parameters in lung homogenates of bifenthrin intoxicated rats. Rats were divided into four groups. Group I served as control while group II animals were treated with bifenthrin @ 5.8mg/Kg/day. In group III, vitamin C was orally administered @ 60mg/Kg/day where as group IV received both vitamin C and bifenthrin @ 60mg/Kg/day and 5.8mg/Kg/day respectively. After 30th day of treatment, lung samples were taken and analysed for oxidative stress parameters. Significant (P<0.05) increase in lipid peroxidation was observed from control value of 4.80±0.39 to 7.90±0.50 in bifenthrin treated animals. Mean control values of SOD, GSH-Px and CAT were 0.55±0.05, 0.98±0.03 and 138.70±6.01 which were significantly (P<0.05) decreased to values of 0.27±0.0, 0.53±0.05 and 91.10±9.70 respectively in bifenthrin treated animals. The value of GST increased significantly (p<0.05) to 1.05±0.06 in bifenthrin administered animals from control value of 0.70±0.08. Pre-treatment with vitamin C in ameliorative group IV significantly restored the normal values of lipid peroxidation, SOD, GST and CAT but could not reverse the decreased values of GSH-Px. The present research is first of its type where in free radical generation due to bifenthrin –a commonly used insecticide was evaluated in lung homogenates when given orally which might be due to residues present in the lung. Besides it will be helpful in better understanding of toxicological profile of pyrethoids, the most commonly used insecticides.
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Bifenthrin, Oxidative stress, Rats, Vitamin C
Aebi, H.E. (1983). Catalase. In Methods Enzymatic analysis, ed.HU. Bergmeyer, New York: Academic Press.
Ahmed, S., Saleem, M.A. and Khurram, R.K. (2004). Effect of cypermethrin (10 EC) and bifenthin (10 EC) on levels of acid and alkaline phosphatases in a strain of tribolium castaneum (Herbst ) (Coleoptera: tenebrionidae). Pak. Entomol. 26: 47-51.
Belma, G., Aylin, G. and Filiz, H. (2001). Cypermethrininduced oxidative stress in rat brain and liver is prevented by vitamin E or allopurinol. Toxicol Lett. 118:139-46.
Bielski, B.H., Richter, H.W. and Chan, P.C. (1975). Some properties of the ascorbate free radical. Ann N Y Acad Sci. 258:231–237
Buettner, G.R., Moseley, P.L. (1993). EPR spin trapping of free radicals produced by bleomycin and ascorbate. Free Radic Res Commun. 19:89 -93.
Cadenas, S., Rojas, C. and Barja, G. (1998). Endotoxin increases oxidative injury to proteins in guinea pig liver: protection by dietary vitamin C. Pharmacol Toxicol. 82:11–18
Chelikani, P., Fita, I. and Loewen, P.C. (2004). Diversity of structures and properties among catalases. Cell. Mol. Life Sci. 61 (2): 192–208.
Chisolm, G.M. and Steinberg, D. (2000). The oxidative modification hypothesis of atherogenesis: an overview. Free Radic Biol Med. 28:1815– 1826
Dar, M.A., Raina , R., Mir, A.H., Sultana, M. and Pankaj, N.K. (2014). Effect of dermal application of bifenthrin on acetylcholinesterase and oxidative stress indices in rat blood, lung and kidney. Proc Natl Acad Sci India Sect B Bio Sci.doi: 10.1007/s4001-014-0390-z.
Dubey, N., Raina, R. and Khan, A.M. (2012). Toxic effects of deltamethrin and fluoride on anti-oxidant parameters in rats. Fluoride. 45: 242-246
Eraslan, G., Kanbur, M. and Silici, S. (2008). Effect of cypermethrin on some biochemical changes in rats: the protective role of prospolis. Exp.Anim. 57: 453-60.
Habig, W.H, Pabst, M.J and Jakoby, W.B. (1974) .Glutathione –S-transferase. The first enzymatic step in mercapturic acid foramtion. J Biol Chem. 249: 7130-7139.
Hafeman, D.G., Sunde, R.A. and Hoekstra, W.G. (1974). Effect of dietry selenium on erythrocyte and liver glutathione peroxidase in rat. J Nutr. 104: 580-587.
Halliwell, B. and Gutterridge, J.M.C. (1989). Free radicals in biology and medicine, 2nd edition. Clarendon press, Oxford.pp 188-276.
Halliwell, B., Zhao, K. And Whiteman, M. (1999). Nitric oxide and peroxynitrite. The ugly, the uglier and the not so good: a personal view of recent controversies. Vitamin C: Antioxidant Actions in Humans. Free Radic Res. 31:651–669
Khan, A.M, Sultana, M., Raina, R., Dubey, N. and Dar, S.A. (2013). Effect of sub acute toxicityof bifenthrin on antioxidant status and haematology after the oral exposure in goats. ProcNatl Acad Sci India Sect B Bio Sci.doi: 10.1007/s4001-01300157-y
Llewellyn, D.M, Brazier, A., Brown, R., Cocker, J., Evans, M.L. and Hampton, J. (1996) Occupational exposure to permethrin during its use as a public hygiene insecticide. Ann Occup Hyg. 40: 499-509.
Manna, S., Bhattacharyya, D., Mandal, T.K. and Das, S. (2005). Repeated dose toxicity of deltamethrin in rats. Indian J Pharmacol. 37: 160-164.
Marklund, S. and Marklund, M. (1974). Involvement of superoxide anion radical in auto-oxidation of pyrogallol and a convenient assay of superoxide dismutase. European J. Biochem. 47: 469-474.
Pesticide Manual (1997). Tomlin CDS,ed. The Pesticide Manual. London: The British Crop Protection Council.
Raina, R., Verma, P.K., Kusum. and Vinay Kant. (2009). Effect of repeated dermal application of ?-cypermethrin on lipid peroxidation and antioxidant system in rats. Toxicol. Int. 16: 27-30.
Sadowska-Woda, I., Popwicz, D. and Karowicz-Billinska, A. (2010). Bifenthrin induced oxidative stress in human erythrocytes and protective effect of selected flavonols. Toxicol In Vitro. 24:460-464.
Shafiq-ur-Rehman. (1984). Lead-induced regional lipid peroxidation in brain. Toxicol Lett. 21 (3): 333-337.
Shakoori, A.R., Naveeda, T. and Sameem, M.A. (1994). Response of melathion-resistant and susceptible strains of Tribolium castaneum (Herbst) to bifenthrin toxicity. Pakistan J.Zool. 26 (2): 169-178.
US Department of Health and Human Services (1993). Hazardous Substances Data Base.HHS. Washington, D
Walker, M.H. and Keith, L.H. (1992). EPA’s Pesticide Fact Sheet Database.Lewis Publishers, Chelsea, MI : 2-12
Yousef, M.I, Awad, T.I. and Mohamed, E.H. (2006). Deltamethrin induced oxidative damage and biochemi-cal alterations in rat and its attenuation by vitamin E. Toxicology. 227(3): 240-247
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