A competent protocol for large scale production of sugarcane (Saccharum officinarum L.) through meristem culture
Article Main
Abstract
A rapid micro propagation and acclimatization response of two different varieties of sugarcane Co86032 and CoN 04131(Saccharum officinarum L.) was obtained in this study. The shoot apical meristem of different sizes wascultured on Murashige and Skoog medium supplemented with different concentrations and combinations of ben-zylaminopurine and kinetin either alone or in combination with each other alongwith GA3. Best shoot formation response in Co 86032 was obtained on MS medium containing 1.5mg/l BAP while in CoN 04131 the combination of 0.5 mg/l BAP with 0.25 mg/l Kinetin showed best shoot formation response from apical meristem. Meristem of 3.0 mm size proved to be the best size for micropropagation of sugarcane. Excellent multiplication response of In vitro formed shoots was obtained when the concentration of BAP was decreased to 1.0 mg/l in Co 86032and 0.25 mg/l BAP and Kin in CoN 04131 (i.e. 0.25 mg/lBAP + 0.25 mg/l Kinetin. MS medium containing 1.0 mg/l NAA and 2.0 mg/l IBA showed 100% rooting response of In vitro regenerated shoots of both the varieties of sugarcane within eight days of inoculation. Best hardening response was obtained in sand+ soil + pressmud (1:1:1) media.
Article Details
Article Details
Benzylaminopurine, Gibberellin, Kinetin, Meristem culture and Naphthalene acetic acid
Ali, A., Naz, S., and Iqbal, J. (2008). An efficient protocol for large scale production of sugarcane through micropropagation. Pak. J. Bot., 40:139-149.
Barba, R.C., Zamora, A.B., Malion, A.K. and Linga, C.K. (1978). Sugarcane tissue culture research. Proc. Int. Soc. Sugarcane Technol.,16:1843-1863.
Biondi, (1986). Practical application of in vitro propagation: Present situation and future prospects. Giom. Bot. Ital., 120:29-42.
Cheema, K.L. and Hussain, M. (2004).Micropropagation of sugarcane through apical bud and axillary bud. Food and agricultural organization of the United Nations., 6 (2): 157-159.
Das, S., Jha, T.B. and Jha, S. (1996). Strategies for improvement of Cashewnut through Tissue Culture.In: Plant Tissue Culture. Islam AS (ed.) Oxford and IBH Publishing Co. Pvt. Ltd, pp. 1-7.
Engelman, F. (1995). Brief overview of IPGRI’s research activities on In vitro conservation of plant species. IPGRI, via delleSetteChiese, Rome, Italy., 2 (1): 9-10.
Flores, S. and Tobin, E.M. (1988).Cytokinin regulation of LHEP mRNA levels.The involvement of post transcriptional regulation.Plant Mol. Biol., 11: 409-415.
Goel, Y., Singh, V.P., Lal, M. and Sharma, M.L. (2010).In vitro morphogenesis in leaf sheath explants of sugarcane hybrid var. CoS 99259.
Guimarces, C.T. and Sobral, W.S. (1998). The Saccharum complex: relation to other and ropogoneae. Plant Breed.Rev., 16: 269-288.
Khattak, W.A., Islam, M.U. and Ullah, U. (2014).Effect of different media concentrations on callogensis in sugarcane (Saccharum officinarum L.).African J. of biotechnology., 13 (11):1219-1222.
Khan, S.A., Rashid, H., Chaudhary, M.F., Chaudhry, Z. and Afroz, A. (2008). Rapid micropropagation of three elite Sugarcane (Saccharum officinarum L.) varieties by shoot tip culture.African Journal of Biotechnology.,7 (13): 2174-2180.
Lorenzo, J.C., Ojeda, E., Espinosa, A. And Borroto, C. (2001). Field performance of temporary immersion bioreactor derived sugarcane plants. In vitro cell Dev. Biol., Plant, 37: 803-806.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol., Plant.,15: 473-487.
Nand, L. and Singh, H.N. (1994).Rapid clonal multiplication of sugarcane through tissue culture.Plant Tissue Cult., 4: 1-7.
Nadgauda, R.S. (2002). Need for tissue culture. Tissue culture pilot plant, National Chemical Laboratory, Pure pp. 1-4.
Pruski, K.T., Astatkie, and Nowak, J. (2005).Tissue culture propagation of Mngoliancherry (Prunusfruticosa) and Nanking cherry (Prunustomentosa) Plant Cell, Tiss.and Org. Cul.,82 (2): 207-211.
Saunders, M.J. and Hepler, P.K. (1982). Calcium ionophore A23187 stimulates cytokininlikemitosis in Funaria. Science, 217: 943-945.
Sauvaire, D. and Glozy, R. (1978). Multiplication vegetative de canne a Sucre (Saccharum sp.) par bouturage in vitro. CR Acad. Sci. Paris, Seri D., pp. 467-470.
Siddiqui, F.A. (1993). A study of the elimination of Sugarcane mosaic virus from Saccharum officinarum by means of in vitro meristem and callus culture and some biochemical aspects of regenerated healthy and infected plants. Ph.D. Thesis.Department of Botany, University of Punjab, Lahore, Pakistan.
Tolera, B., Diro, M., and Belew, D. (2014).Response of Sugarcane (Saccharum officinarum L.)Varieties to BAP and Kinetin on in vitro Shoot Multiplication.International J. of Innovative Research and development., 3(5): 694-695.
Vervoodre, P. and Grimes, H.D. (1994). The role of calcium and calmodulin in carrot somatic embryogenesis. Plant Cell Physiol., 35: 135-144.
Weiler, E.W. (1984). Immunoasssay of plant growth regulators. Ann. Review of Plant Physiol.,35: 85-95.
Yi, M., Guo, Y., Yang, Z., Guo and Chen, G. (2004). Adventitious shoot bud formation and plant regeneration from in vitro-cultured stem segments of reed (Phragmitescommunis Trin.). In vitro cellular and developmental Biol., 40 (4): 412-415.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)