Quantitative changes in phytochemicals in tomato plant due to application of resistance inducing chemicals and their role in inhibition of early blight pathogen Alternaria alternata
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Abstract
Phytochemicals viz. soluble protein, reducing sugar and phenols were quantified from tomato (Solanum lycopersicon) leaves after application of resistance inducing chemicals viz. salicylic acid, ?-aminobutyric acid, chitosan and 2,6- dichloroisonicotinic acid as 8 hr seed dip treatment or 2 hr seedling dip treatment or both treatment to study their effect on induction of resistance and inhibition of growth of pathogen. Soluble proteins and phenols were found maximum due to seed+seedling treatment of salicylic acid @ 1.5 mM concentration with 76.90 per cent and 102.68 per cent increase over control whereas reducing sugar was maximum for seed+seedling treatment of ?-aminobutyric acid @ 15.0 mM concentration with 61.38 per cent increase over control. The increased level of protein quantity had no effect on inhibition of Alternaria alternata growth, whereas the increased quantity of sugar inhibited the average growth of Alternaria up to 19.39 per cent. Among phenolic compounds catechol and the cinnamic acid (formed in shikimic acid pathway of phenol biosynthesis) was inhibitory to the A. alternata whereas tannic acid had
some effect on inhibition of Alternaria growth (13.84 % fungal growth inhibition). The increased level of sugar+phenol tested against the pathogen completely inhibited the growth of Alternaria fungus. Thus, the increased level of reducing sugar and phenol in tomato leaves due to the application of resistance inducing chemicals seems
to be inhibitory to the pathogens multiplication and pathogenesis.
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Alternaria alternata, Inhibition, Phytochemicals, Resistance inducing chemicals
Arici S.E. and H.W. Dehne. (2007). Induced resistance against Phytophthora infestans by chemical inducers Bion and BABA in tomato plants. Acta Hort. (ISHS)., 729:503-507.
Ashry N.A. and H.I. Mohamed (2012). Impact of secondary metabolites and related enzymes in flax resistance and/or susceptibility to powdery mildew. African J. Biotechnol., 11(5): 1073-1077.
Barilli E., Sillero J.C. and D. Rubiales. (2009). Chemical induction of SAR in pea (Pisum sativum L.) against pea rust enhances antifungal activity and accumulation of phenolic compounds. Induced Resistance in Plants against Insects and Diseases – Chances and Limits. 5th Meeting of the IOBC Working Group “Induced Resistance in Plants against Insects and Diseases” Granada, Spain, 12-16 May 2009, pp.29.
Bennett R.N. and R.M. Wallsgrove. (1994). Secondary metabolites in plant defence mechanisms. New Phytologist, 127 (4): 617–63.
Cheng-bo Y.I. and Y.E. Hua-zhi. (2005). Induced resistance of maize against Curvularia lunata with exogenous chemical compounds[J]. Plant Protec. pp.05.
Esmailzadeh, M., M.J. Soleimani and H. Rouhani. (2008). Exogenous applications of salicylic acid for inducing systemic acquired resistance against tomato stem canker disease. J. Boil. Sci., 8: 1039-1044.
Flors V., M.C. Miralles, E. Varas, P. Company, C. Gonzalez-Bosch and P. Garcia-Agustin. 2004. Effect of analogues of plant growth regulators on in vitro growth of eukaryotic plant pathogens. Plant Pathol., 53(1): 58–64.
Friedrich L., Lawton K., Ruess W., Masner P., Specker N., Rella M.G., Meier B., Dincher S., Staub T., Uknes S., Métraux J.P., Kessmann H. and J.A. Ryals. (1996). Benzothiadiazole derivative induces systemic acquired resistance in tobacco. Plant J., 10: 61-70.
Hadi M.R. and G.R. Baladi. (2010). The effect of salicylic nacid on the reduction of Rhizoctonia solani damage in the tubers of Marfona potato cultivar. American-Eurasian J. Agric. Environ. Sci., 7(4): 492-496.
Jakab G., Cottier V., Toquin V., Rigoli G., Zimmerli L., Metraux J.P., and B. Mauch-Mani. (2001). Betaaminobutyric acid-induced resistance in plants. Eur. J. Plant Pathol., 107:29-37.
Ju Shu L.I., M.A. De hua, Pang Jin an and Huo Zhen Rong. (2002). Induced effect of salicylic acid on the activity of several enzymes and disease resistance of cucumber. Acta Agriculturae Boreall—Sinica. pp. 02.
Kefeli V.I. and M. Kutacek . (1977). Phenolic Substances and Their Possible Role in Plant Growth Regulation. Plant Growth Regulation, Proceedings in Life Sciences. pp 181-188. Lafontaine P.J. and N. Benhamou. (1996). Chitosan treatment: An emerging strategy for enhancing resistance of greenhouse tomato plants to infection by Fusarium oxysporum f.sp. radicis-lycopersici. Biocontrol Sci. Tech., 6(1): 111-124.
Lowry O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265.
Maddox C.E., Laur L.M. and Tian L. (2010). Antibacterial activity of phenolic compounds against the phytopathogen Xylella fastidiosa. Curr Microbiol., 60(1):53-58.
Moghaddam M. R. B. and Ende W. Van den. (2012). Sugars and plant innate immunity. J. Exp. Bot., 63 (11): 3989-3998.
Muzzarelli R.A.A., Muzzarelli C., Tarsi R., Miliani M., Gabbanelli F. and M. Cartolari. (2001). Fungistatic activity of modified chitosans against Saprolegnia parasitica. Biomacromol., 2: 165–169.
Panina Y., D.R. Fravel, C.J. Baker and L.A. Shcherbakova. (2007). Changes in phenolic compounds in tomato in response to biocontrol and plant pathogenic Fusarium oxysporum. http://www.mbao.org/2006/06 Proceedings /134Fravel-mbao-06.pdf.
Polyakovskii S.A., Z.N. Kravchuk, A.P. Dmitriev. (2008). Mechanism of action of the plant resistance inducer beta-aminobutyric acid in Allium cepa. Cytology and Genetics., 42: 369-372.
Pospiezny H., S. Chirkov and J. Atabekov. (1991). Induction of antiviral resistance in plants by chitosan. Plant Sci., 79 (1): 63-68.
Reddy M.V.B., J. Arul, P. Angers, and L. Couture. (1999). Chitosan treatment of wheat seeds induces resistance to Fusarium graminearum and improves seed quality. J. Agric. Food Chem., 47 (3):1208–1216.
Rodaki A., I.M. Bohovych, B. Enjalbert, T. Young, F.C. Odds, N. A.R. Gow and A. J.P. Brown. (2009). Glucose Promotes Stress Resistance in the Fungal Pathogen Candida albicans. Mol. Biol. Cell, 20 (22): 4845-4855.
Rosa M., C. Prado, G. Podazza, R. Interdonato, J.A. González, M. Hilal, F. E. Prado. (2009). Soluble sugars Metabolism, sensing and abiotic stress: A complex network in the life of plants. Plant Signal Behav. 4(5): 388–393.
Samia M. and El- Khallal. (2007). Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (jasmonic acid& salicylic acid):1-changes in growth, some metabolic activities and endogenous hormones related to defence mechanism. Australian J.Basic Appl. Sci., 1(4): 691-705.
Si-Ammour A., Mauch-Mani B. and F.Mauch. (2003). Quantification of induced resistance against Phytophthora species expressing GFP as vital marker: b-aminobutyric acid but not BTH protects potato and Arabidopsis from infection. Mol. Plant Pathol., 4(4): 237–248.
Somogyi M. (1952). Notes on sugar determination. J. Biol. Chem., 195: 19-23.
Spletzer M.E. and A.J. Enyedi. (1999). Salicylic acid induces resistance to Alternaria solani in hydroponically grown tomato. Bioch. Cell Biol., 89: 722-727.
Swain J. and W.E. Hills. (1959). The phenolic constituents of prumas domestical. The qualitative analysis of phenolic constituents. J. Sci. Food Agric., 10: 63.
Wu H.S., Liu Y.D., Yang X.I., Chen X.Q., Wang Z.H., Kong X.Y., Liu X.X. and S. Yan. (2010). Growth responses of in vitro Fusarium oxysporum f. sp. niveum to external supply of tannic acid. J Environ Biol., 31(6):1017-1022.
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