##plugins.themes.bootstrap3.article.main##

Anurag Yadav Kusum Yadav Anupam Vashistha

Abstract

A study was designed to screen and analyze the efficient phosphate solubilizing bacteria (PSBs) from wheat rhizosphere. Five biovars of Pseudomonas fluorescens (PSM1, PSM2, PSM3, PSM4 and PSM5) were isolated from wheat rhizosphere and Bacillus megaterium MTCC 8755 procured from microbial type culture collection (MTCC) Chandigarh, India. The P. fluorescens biovar PSM1 was observed to be most efficient phosphate solubilizer. Inoculation of P. fluorescens PSM1 and B. megaterium MTCC 8755, alone and in combination, caused the highest phosphate solubilization at pH 5. At this pH, maximum phosphate solubilization was observed with B. megaterium MTCC 8755 inoculation (8.2 mg mL-1) on sixth day of incubation with P. fluorescens PSM1 (8 mg mL-1) on seventh day of incubation and with dual bacterial treatment (10.5 mg mL-1) on the fourth day of incubation. A correlation coefficient of linear regression equation of phosphate solubilization with pH indicated that pH value of the medium was directly correlated with tricalcium phosphate solubilization. The study will help in choosing soil pH specific PSB inoculant for optimizing plant growth.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Phosphate solubilizing bacteria, Pseudomons fluorescens, Rhizosphere

References
Aftab Afzal, A.B. (2008). Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticum aestivum L.). Int J Agric Biol. Int. J. Agri. Biol, 10(1): 1560-8530.
Bergey, D.H. and Holt, J. (2000). Bergey's manual of determinative bacteriology, 9 ed. Williams & Wilkins, Philadelphia.
Corbridge, D.E.C. (1985). Phosphorus: an outline of its chemistry, biochemistry, and technology. Elsevier Science Publishers.
Dawson, R.M., Dawson, R.M.C., Elliot, D.C., Elliot, W.H. and Jones, K.M. (1986). Data for Biochemical Research, 3 ed. Oxford Science Publications.
Gaind, S. and Gaur, A. (1989). Effect of pH on phosphate solubilization by microbes. Current Science, 58(21): 1208-1211.
Gulati, A., Sharma, N., Vyas, P., Sood, S., Rahi, P., Pathania, V. and Prasad, R. (2010). Organic acid production and plant growth promotion as a function of phosphate solubilization by Acinetobacter rhizosphaerae strain BIHB 723 isolated from the cold deserts of the trans-Himalayas. Archives of Microbiology, 192(11): 975-983.
Hassani, F., Asgharzade, A., Ardakani, M., Hamidi, A. and Paknejad, F. (2015). Effectiveness of phosphate solubilizing bacteria inoculation for improving phosphorus absorption and root growth indices. Research Trend, p.199.
Kang, S.-M., Khan, A.L., Hamayun, M., Shinwari, Z.K., Kim, Y.-H., Joo, G.-J. and Lee, I.-J. (2012). Acinetobacter calcoaceticus ameliorated plant growth and influenced gibberellins and functional biochemicals. Pak. J. Bot, 44(1): 365-372.
Kang, S.C., Ha, C.G., Lee, T.G. and Maheshwari, D.K. (2002). Solubilization of insoluble inorganic phosphates by a soil-inhabiting fungus Fomitopsis sp. PS 102 Current Science, 82(4): 439-442.
King, E.O., Ward, M.K. and Raney, D.E. (1954). Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med, 44(2): 301-307.
Kloepper, J.W., Lifshitz, R. and Zablotowicz, R.M. (1989). Free-living bacterial inocula for enhancing crop productivity. Trends in Biotechnology, 7(2): 39-44.
Krishnaveni, M.S. (2010). Studies on phosphate solubilizing bacteria (psb) in rhizosphere and non-rhizosphere soils in different varieties of foxtail millet (Setaria italica). International Journal of Agriculture and Food Science Technology, 1(1): 23-39.
Lundblad, R.L. and Macdonald, F. (2010). Handbook of Biochemistry and Molecular Biology, Fourth Edition. Taylor & Francis.
Maheswar, N.U. and Sathiyavani, G. (2012). Solubilization of phosphate by Bacillus sps, from groundnut rhizosphere (Arachis hypogaea L.) Journal of Chemical and Pharmaceutical Research, 4(8): 4007-4011.
Marra, L.M., Oliveira-Longatti, S.M.d., Soares, C.R.F.S., Lima, J.M.d., Olivares, F.L., Moreira, F.M.S., Marra, L.M., Oliveira-Longatti, S.M.d., Soares, C.R.F.S., Lima, J.M.d., Olivares, F.L. and Moreira, F.M.S. (2015). Initial pH of medium affects organic acids production but do not affect phosphate solubilization. Brazilian Journal of Microbiology, 46(2): 367-375.
Najjar, G., Godlinski, F., Vassilev, N. and Eichler-Löbermann, B. (2012). Dual inoculation with Pseudomonas fluorescens and arbuscular mycorrhizal fungi increases phosphorus uptake of maize and faba bean from rock phosphate. vTI Agriculture and Forestry Research: 77.
Netik, A., Torres, N.V., Riol, J.M. and Kubicek, C.P. (1997). Uptake and export of citric acid by Aspergillus niger is reciprocally regulated by manganese ions. Biochimica Et Biophysica Acta, 1326(2): 287-294.
Park, J., Bolan, N., Mallavarapu, M. and Naidu, R. (2010). Enhancing the solubility of insoluble phosphorus compounds by phosphate solubilizing bacteria, 19th World Congress of Soil Science, Soil Solutions for a Changing World Brisbane, Australia.
Prasanna, A., Deepa, V., Balakrishn, P., Deecaraman, M., Sridhar, R. and Dhandapani, P. (2011). Insoluble phosphate solubilization by bacterial strains isolated from rice rhizosphere soils from southern India. International Journal of Soil Science, 6(2): 134-141.
Roychoudhury, P. and Kaushik, B.D. (1989). Solubilization of Mussorie rock phosphate by cyanobacteria. Current Science, 58: 569-570.
Subba Rao, N.S. (1982). Advances in agricultural microbiology, London (UK), Butterworth.
Welch, S.A., Taunton, A.E. and Banfield, J.F. (2002). Effect of microorganisms and microbial metabolites on apatite dissolution. Geomicrobiology Journal, 19: 343-367.
Yoshikawa, M., Hirai, N., Wakabayashi, K., Sugizaki, H. and Iwamura, H. (1993). Succinic and lactic acids as plant growth promoting compounds produced by rhizospheric Pseudomonas putida. Can J Microbiol, 39 (12): 1150-1154.
Citation Format
How to Cite
Yadav, A., Yadav, K., & Vashistha, A. (2016). Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere. Journal of Applied and Natural Science, 8(1), 93–96. https://doi.org/10.31018/jans.v8i1.754
More Citation Formats:
Section
Research Articles