Determination of the role of Dermococcus nishinomiyaensis and Pseudomonas aeruginosa in polyethylene (PE) and polyethylene terephthalate (PT) biodegradation
Article Main
Abstract
Plastic pollution is a worldwide menace to the ecosystem and human health, and only less than 10 percent of the plastic waste has been recycled and its continued existence causes climate change. The purpose of the study was to test the potential of biodegradation of Dermococcus inshinomiyaensis on two common types of plastics (Polyethylene Terephthalate (PET) and Polyethylene (PE)) when compared to the established degrader, Pseudomonas aeruginosa, at conditions that simulated the natural environment. The biodegradation was evaluated in terms of biofilm biomass determination, relative weight loss, scanning electron microscopy (SEM), and Fourier-transform infrared (FT-IR) spectroscopy. Biofilm quantification showed that D. inshinomiyaensis formed stronger biofilms on PET bottles (absorbance 0.16) than on PE bags (0.10–0.12), with activity comparable to that of P. aeruginosa. The analysis of weight loss showed that PE was severely degraded by D. inshinomiyaensis (12.2%), P. aeruginosa (40%), and PET was not severely degraded (0.7%). FT-IR spectra of PE indicated new absorption bands beyond 400 cm-¹, confirming structural defects induced by microbial activity. Distinct peaks between 1600–2653 cm-¹, including 1639 and 1737 cm-¹ for P. aeruginosa and 1981–2325 cm-¹ for D. inshinomiyaensis, suggested the formation of novel intermediates. SEM analysis also showed intense deformations in PE using D. inshinomiyaensis so that it generated a wide range of cracks, wrinkles, and grooves, which were much more effective compared to the action of P. aeruginosa.These results demonstrate the novelty of D. inshinomiyaensis as a potential biodegrader of PE, as it provided potential use in the management of plastic waste in a sustainable environment.
Article Details
Article Details
Biodegradation, Fourier-transform infrared, Polyethylene, Sustainable development, Terephthalate
Bardají, D. K. R., Furlan, J. P. R. & Stehling, E. G. (2019). Isolation of a polyethylene degrading Paenibacillus sp. from a landfill in Brazil. Archives of Microbiology, 201(5), 699-704. 10.1007/s00203-019-01637-9
Bolo, N. R., Diamos, M. A. J. C., SIA, S., Ocampo, M. A. B. & Suyom, L. M. (2015). Isolation, identification, and evaluation of polyethylene glycol and low density polyethylene-degrading bacteria from Payatas Dumpsite, Quezon City, Philippines. Phil. J. Health Res. Dev., 19 (1), 50-59.
Chen, Z., Zhao, W., Xing, R., Xie, S., Yang, X., Cui, P., ... & Zhou, S. (2020). Enhanced in situ biodegradation of microplastics in sewage sludge using hyperthermophilic composting technology. Journal of Hazardous Materials, 384, 121271. https://doi.org/10.1016/j.jhazmat.20 19.121271
Das, M. P. & Kumar, S. (2015). An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech., 5(1), 81-86. DOI: 10.1007/s13205-014-0205-1
Dwicania, E., Rinanti, A. & Fachrul, M. F. (2019, December). Biodegradation of LLDPE plastic by mixed bacteria culture of Pseudomonas aeruginosa and Brevibacterium sp. In Journal of Physics: Conference Series (1402, 2, 022105). IOP Publishing. DOI: 10.1007/s12088-012-0250-6
Gupta, K. K. & Devi, D. (2020). Characteristics investigation on biofilm formation and biodegradation activities of Pseudomonas aeruginosa strain ISJ14 colonizing low density polyethylene (LDPE) surface. Heliyon, 6(7). DOI: 10.1016/j.heliyon.2020.e04398
Han, Y. N., Wei, M., Han, F., Fang, C., Wang, D., Zhong, Y. J.& Li, F. M. (2020). Greater biofilm formation and increased biodegradation of polyethylene film by a microbial consortium of Arthrobacter sp. and Streptomyces sp. Microorganisms, 8(12), 1979. DOI: 10.3390/microorganisms8121979
Jung, J. W., Kang, J. S., Choi, J. & Park, J. W. (2020). Chronic toxicity of endocrine disrupting chemicals used in plastic products in Korean resident species: Implications for aquatic ecological risk assessment. Ecotoxicology and Environmental Safety, 192, 110309. DOI: 10.1016/j.ecoenv.2020.110309
Kim, H. R., Lee, H. M., Yu, H. C., Jeon, E., Lee, S., Li, J. & Kim, D. H. (2020). Biodegradation of polystyrene by Pseudomonas sp. isolated from the gut of superworms (larvae of Zophobas atratus). Environmental Science & Technology, 54(11), 6987-6996. DOI: 10.1021/acs.est.0c01495
Kong, D., Wang, L., Chen, X., Xia, W., Su, L., Zuo, F., ... & Wu, J. (2022). Chemical-biological degradation of polyethylene combining Baeyer–Villiger oxidation and hydrolysis reaction of cutinase. Green Chemistry, 24(5), 2203-2211. https://doi.org/10.1039/D2GC00425A
Lahive, E., Walton, A., Horton, A. A., Spurgeon, D. J. & Svendsen, C. (2019). Microplastic particles reduce reproduction in the terrestrial worm Enchytraeus crypticus in a soil exposure. Environmental Pollution, 255, 113174. https://doi.org/10.1016/j.envpol.2019.113174
Lee, H. M., Kim, H. R., Jeon, E., Yu, H. C., Lee, S., Li, J. & Kim, D. H. (2020). Evaluation of the biodegradation efficiency of four various types of plastics by Pseudomonas aeruginosa isolated from the gut extract of superworms. Microorganisms, 8(9), 1341. doi.org/10.3390/microorganisms8091341
Lv, S., Li, Y., Zhao, S. & Shao, Z. (2024). Biodegradation of typical plastics: from microbial diversity to metabolic mechanisms. International Journal of Molecular Sciences, 25(1), 593. https://doi.org/10.3390/ijms25010593
Tari V. S.& Kannan, K. (2022). "Microbial bioremediation of polythene and plastics: a green sustainable approach" In a Malik, J. A. (Ed.). Microbes and Microbial Biotechnology for Green Remediation, p550. Elsevier. 10.1016/B978-0-323-90452-0.00003-7
Mir, S., Asghar, B., Khan, A. K., Rashid, R., Shaikh, A. J., Khan, R. A. & Murtaza, G. (2017). The effects of nanoclay on thermal, mechanical and rheological properties of LLDPE/chitosan blend. Journal of Polymer Engineering, 37(2), 143-149. 10.1515/polyeng-2015-0350
Mohammad, G.A. & Taha Daod, S. (2021) Impact of tween 80 on fatty acid composition in two bacterial species. Archives of Razi Institute, 76(6), pp. 1617–1627. 10.22092/ari.2021.356359.1826
Mohammad, G.A.; Al-Ani, A.G.&Al-Taee, S.M. (2024). Assessing the common ancient traditions prescribed by herbalists for otitis media treatment. Malaysian Journal of Microbiology, 20(5),. 614-622. DOI: http://dx.doi.org/10.21161/mjm.230187. 10.21161/mjm.230187
Mahmoud, A. & Mohammad, G. (2024). Curing of Citrobacter spp. strains antibiotic resistance harboring plasmids using novel physical, chemical and natural substances. Egyptian Journal of Veterinary Sciences, 55(7), 2107-2118. 10.21608/EJVS.2024.270065.1844
Moharir, R. V. & Kumar, S. (2019). Challenges associated with plastic waste disposal and allied microbial routes for its effective degradation: A comprehensive review. Journal of Cleaner Production, 208, 65-76. 10.1016/j.jclepro.2018.10.059
Raziyafathima, M. P. P. K., Praseetha, P. K. & Rimal, I. R. S. (2016). Microbial degradation of plastic waste: a review. J. Pharma Chem. Biol. Sci., 4, 231-242.
Royer, S. J., Ferrón, S., Wilson, S. T.& Karl, D. M. (2018). Production of methane and ethylene from plastic in the environment. PloS One, 13(8). 10.1371/journal.pone.0200574
Ru, J., Huo, Y. & Yang, Y. (2020). Microbial degradation and valorization of plastic waste. Frontiers in Microbiology, 11, 442. https://doi.org/10.3389/fmicb.2020.00442
Shah, A. A., Hasan, F., Hameed, A. & Ahmed, S. (2008). Biological degradation of plastics: A comprehensive review. Biotechnology Advances, 26(3), 246–265. https://doi.org/10.1016/j.biotechadv.2007.12.005
Urbanek, A. K., Rymowicz, W. & Mirończuk, A. M. (2018). Degradation of plastics and plastic-degrading bacteria in cold marine habitats. Applied Microbiology and Biotechnology, 102(18), 7669–7678. https://doi.org/10.1007/s00253-018-9195-y.
Webb, H. K., Arnott, J., Crawford, R. J. & Ivanova, E. P. (2012). Plastic degradation and its environmental implications with special reference to poly (ethylene terephthalate). Polymers, 5(1), 1-18. 10.3390/polym5010001
Zhang, J., Gao, D., Li, Q., Zhao, Y., Li, L., Lin, H., ... & Zhao, Y. (2020). Biodegradation of polyethylene microplastic particles by the fungus Aspergillus flavus from the guts of wax moth Galleria mellonella. Science of the Total Environment, 704, 135931. 10.1016/j.scitotenv.2019.135931
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
