Wayde Jitendra Eknath Akshay S. Dange Trishala A. Pagar Deepak Rameshwar Sapkal Monika Bansal


Pomegranate (Punica granatum L.) is an important fruit crop of India and other subtropical countries. Bacterial blight caused by Xanthomonas axonopodis pv. Punicae is becoming a major threat in Maharashtra as well as other states of India. It causes yield losses up to the extent of 80 – 90 %. This study aims to the collection of 7 strains (X. axonopodis) and utilized for assessment of genetic diversity by using RAPD markers. Whereas 7 strains of X. axonopodis were differentiated and distinguished into seven major region representing Malegaon, Nampur, Satana, Khakurdi, Ramnagar, Rahuri and Nashik region. However the genetic similarity within 7strains of X. axonopodis was ranged between 72.72-100 %. Similarly the genetic relationship of strains X. axonopodis was also established and showed 72.72 %, 72.72 %, 72.72 % 92.30%, 94.11% and 100 % similarity with region of Malegaon, Nampur, Satana, Khakurdi, Ramnagar, Rahuri and Nashik region. Average polymorphism in strains of X. axanopodis based on RAPD analysis was 84.095 %. The combine data analysis by using UPGMA method also revealed the two distinct groups representing seven strains of X. axonopodis and in first group M1 and second group R4 and subgroup RH6, N2, NS7, S3, K5, these genotypes were present respectively. Considering predominance and economic loss caused by X. axonopodis pv. punicae in pomegranate, it is an essential to undertake preliminary work on characterization of this pathogen at the molecular level.




Bacterial blight, Diversity, Oily spot pathogen, Pomegranate, RAPD

Abdo-Hasan, N.M., Khalil, H., Debis, B. and Mir-Ali, N. (2008). Molecular characterization of Syrian races of Xanthomonas axonopodis pv.malvacearum. J. Pl. Pathol. 90 (3): 431-439.
Chand, R. and Kishan, R. (1991).Studies of bacterial blight (Xanthomonas campestris pv. punicae) of pomegranate. Indian Phytopathol. 44 (3): 370-372.
Dhutraj D.N. and Soryawansbi A.P., (2010) Stanadardization of inoculum techniques for Xanthomonas Axonopodis PV. Vesicatoria. Journal of Plant disease Sciences.5 (2):214-216.
Edivanio, R. A., Marisa, A. S. V., Ferreira, and Alice M.Q.D (2013) Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent. Eur J Plant Pathol. 137:5–9.
Gupta, V.S., Rajebhosale, M.D., Sodhi, M., Singh, S., Gnanamanickam, S. S.,Dhaliwal, H. S. and Ranjekar, P.K. (2001). Assessment of genetic variability and strain identification of Xanthomonas oryzae pv. Oryzae using RAPD-PCR and IS1112 based PCR. Cur. Sci. 80: 8-25.
Hingorani, M.K. and Mehta, P.P. (1952). Bacterial leaf spot of pomegranate. Indian Phytopathol. 5 :55-56.
Jyoti, S.V. Krishnappa, M. and Reddy Krishna, M. (2005). Detection of X. axanopodis pv. punicae on pomegranate (Punica granatum L.) in Karnataka. J. of Mycol. and Pl. Pathol. 35(3): 518-519.
Kanwar, Z.S. (1976). A note on disease of pomegranate (Punica granatum) Hariyana. J. Horticul. Sci. 5: 171-180.
Kumar, R., Shamarao, J.M.R., Yenjerappa, S.T., Patil, H.B. (2009). Epidemiology and management of bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. Punicae. Acta Horticul. 818:291-296.
Manjula, C.P. and Khan, A.N.A. (2003). Incidence of bacterial blight of pomegranate (Punica granatum L.) in Karnataka. Indian Phytopathol. 56(3): 341
Manulis, S., Valinsky, L., Lichter, A. and Gabrie, D.W. (1994) Sensitive and specific detection of Xanthomonas Campestris cv. Pelargonii with DNA primers and probes identified by Random Amplified Polymorphic DNA Analysis. Appl. and Environ. Microbiol. 60(11): 4094-4099.
Mondal, K.K. and Sharma, J. (2009). Bacterial blight: An emerging threat to pomegranate export. Indian Farming 59: 22-23.
Mondal, K.K. and Singh, D. (2009). Bacterial blight of pomegranate-A technical bulletin. Division of Plant Pathology. Indian Agric. Res. Inst. New Delhi. : 8.
Mondal, K.K. and Mani, C. (2009). ERIC-PCR generated genomic fingerprints and their correlation with pathogenic variability of Xanthomonas campestris pv. punicae, the incitant of bacterial blight of pomegranate. Curr. Microbiol. 59: 616-620.
Mondal, K.K., Rajendran, T.P., Phaneendra, C., Mani, C., Sharma, J., Sharma, R., Pooja., Verma, G., Kumar, R., Singh, D., Kumar, A., Saxena, A. K. and Jain, R.K. (2012). The reliable and rapid polymerase chain reaction (PCR) diagnosis for Xanthomonas axonopodis pv. Punicae in pomegranate. African J. Microbiol. Res. 6 (30): 5950-5956.
Murray. M.G., and Thompson W.F. (1980): Rapid isolation of high molecular weight DNA. Nucleic. Acids Res. 8: 4321.
Parkinson, N., Cowie, C., Heeney. J. and Stead, D. (2009). Phylogenetic structure of Xanthomonas determined by comparison of gyrB sequences. Int. J. Syst. Evol. Microbiol. 59 : 264-274.
Petersen, Y., Mansvelt, E.L., Venter, E. and Langenhoven, W.E. (2010). Detection of Xanthomonas axonopodis pv. punicae causing bacterial blight on pomegranate in South Africa. J. Austrian Pl. Pathol. 39:544-546.
Sambrook, J.E, Fritsch, E.F. and Maniatis, T.A. (1989) Molecular Cloning: A Laboratory Manual, 2nd edition. Cold Spring Harbor Laboratory, Cold Spring Harbor.
Sabin F , Rukhsana b , tehmina and zafar s (2012). Assessment of genetic diversity among different indigenous Xanthomonas isolates via RAPD and ISSR. Arch. Biol. Sci., Belgrade 64 (1): 307-319.
Sharma, K.K. (2006). Important diseases, disorders and insect pests of Pomegranate and their management. Tech. Bull. NRCP, Solapur. : 2-3.
Sharma, K.K., Sharma. J., Jadhav, V.T. and Chandra, R. (2008). Bacterial blight of pomegranate and its management. Indian Pytopathol. 61 (3) :380-381.
Vauterin, L., Hoste, B., Kesters, K. and Swing, J. (1995). Reclassification of Xanthomonas. Int. J. of Systemic Bacteriol. 45 :472-489.
Versalovic, J. and Lupski, J. (2002). Molecular detection and genotyping of pathogens more accurate and rapid answers. Trends Microbiol. 10 (10):S15-21
Raut, B.T., Anahosur, K.H. and Chavan, P.N. (1993). Standardization of inoculation methods and evaluation of mango genotypes against Xanthomonas compestris pv. Mangiferae indicae, leaf spot causing organism of mango. Indian Phytopathol. 46 :326.
Research Articles

How to Cite

Assessment of the genetic diversity among oily spot (Xanthomonas axonopodis pv. punicae) pathogen of pomegranate by randomly amplified polymorphic DNA analysis. (2015). Journal of Applied and Natural Science, 7(2), 1006-1011. https://doi.org/10.31018/jans.v7i2.722