Biocontrol potential of Flemingia wightiana: A natural weapon against Culex quinquefasciatus
Article Main
Abstract
Globally, mosquito-borne diseases, particlulalry those transmitted by Culex quincquefasciatus pose a significant public health challenge. Traditional methods of eradication using synthetic insecticides pose environmental concerns and a risk of developing insecticide-resistant varieties. Here, the use of plant-based biopesticides offers a safer and sustainable alternative. The study aimed to investigate the insecticidal properties of Flemingia wightiana (FW) leaves by synthesising leaf extracts and silver nanoparticles. The toxicity of the test samples was tested on Oreochromis niloticus at concentrations of 0.1, 0.5, and 1 mg/L. Furthermore, the test samples were subjected to lethality assay on C. quinquefasciatus. Laboratory bioassays were conducted to evaluate the efficacy of crude extract and silver nanoparticles of F. wightiana at varying concentrations, specifically 0.5, 1, 2, and 4 mg/L. Ovicidal, emergency and larvicidal activity were studied. The results indicated significant larvicidal activity and exhibited better potential for toxicity against Culex larvae treated with AgNPs. FW-AgNPs have substantial effect in delaying the hatching of mosquito eggs. Moulting of larvae from one instar to the next was also delayed by treatment with AgNPs. The findings demonstrated that FW-AgNPs play a significant role in controlling C. quinquefasciatus populations.
Article Details
Article Details
Keywords
Culex mosquito, Larvicide, Methanolic extract, Nanoparticles, Phytocompounds, Sustainability
References
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Chacko, L., Poyyakkara, A., Kumar, V. B. S. & Aneesh, P. M. (2018). MoS2-ZnO nanocomposites as highly functional agents for anti-angiogenic and anti-cancer theranostics. Journal of Materials Chemistry. B, Materials for Biology and Medicine, 6(19), 3048–3057. https://doi.org/10.1039/c8tb00142a
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Gavade, S. K., Surveswaran, S., Van der Maesen, L. J. G., & Lekhak, M. M. (2019). Taxonomic revision and molecular phylogeny of Flemingia subgenus Rhynchosioides (Leguminosae). Blumea-Biodiversity, Evolution and Biogeography of Plants, 64(3), 253-271.
( https://doi.org/10.3767/blumea.2019.64.03.06
George, J. A. & Paari, K. A. (2023). Bacillus velezensis-synthesized silver nanoparticles and its efficacy in controlling the Aedes aegypti. Bulletin of Materials Science (India), 46(3). https://doi.org/10.1007/s12034-023-03023-0
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Government of India, Ministry of fisheries, Animal husbandry and dairying, Department of animal husbandry and dairying, Committee for the purpose of control and supervision of experiments on animals (CPCSEA). (2021). Guidelines of CPCSEA for experimentation onfishes.https://ccsea.gov.in/WriteReadData/userfiles/file/Guidelines%20of%20CPCSEA%20for%20Experimentation%20on%20Fishes-2021.pdf
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Kaur, S. & Kaur, A. (2021). Effect of acute doses of basic violet-1 (Bv-1) On antioxidant/detoxification enzymes of Labeo rohita. Uttar Pradesh Journal Of Zoology, 42(24), 936–946. https://iqac.khalsacollege.edu.in/SSR/admin/pdf/pdf_1689397010.pdf
Khan, H. S., Tariq, M., Mukhtar, T. & Gulzar, A. (2021). Insecticidal toxicity of plant extracts and green silver nanoparticles against Aedes albopictus. Pakistan Journal of Zoology, 53(6). https://doi.org/10.17582/journal.pjz/20191214051208
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Kumar, D., Kumar, P., Singh, H. & Agrawal, V. (2020). Biocontrol of mosquito vectors through herbal-derived silver nanoparticles: prospects and challenges. Environmental Science and Pollution Research International, 27(21), 25987–26024. https://doi.org/10.1007/s11356-020-08444-6
Kumar, K. R., Nattuthurai, N., Gopinath, P. & Mariappan, T. (2015). Synthesis of eco-friendly silver nanoparticles from Morinda tinctoria leaf extract and its larvicidal activity against Culex quinquefasciatus. Parasitology Research, 114(2), 411–417. https://doi.org/10.1007/s00436-014-4198-9
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Biocontrol potential of Flemingia wightiana: A natural weapon against Culex quinquefasciatus. (2025). Journal of Applied and Natural Science, 17(4), 1727-1737. https://doi.org/10.31018/jans.v17i4.6785
