Energy dispersive X-ray Spectroscopy technique as a taxonomic indicator in the classification of Klanchoe blossfeldiana Poelln. cultivars
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Abstract
Determining elemental mapping in living plant samples using the energy–dispersive X-ray Spectroscopy technique (SEM-EDS) can be used as an indicator to solve classification problems among closely related taxonomic ranks, such as cultivars and other intraspecific categories. Four cultivars of Klanchoe blossfeldiana Poelln.: K. blossfeldiana Clandiva Monro ( white petals), K. blossfeldiana Clandiva Hayworth (red petals), K. blossfeldiana Clandiva Fonda ( yellow petals ), and K. blossfeldiana Clandiva Bardot (orange petals) were investigated by ( SEM – EDS ) to explain element distribution in relation to petals colour to find out similarities and differences among these cultivars. All the investigated elements were common in all studied cultivars' petals except ( Al3+) ; ( Si3+) ; ( Ni2+ ) were absent in some studied cultivars and present in the others . Scanning electron microscope (SEM) Microphotograph of four K. blossfeldiana Cultivars petals showed different patterns from layer composition of zigzag form and very fine structures in K. blossfeldiana Clandiva Monro to regular composition of circular grooves and chains of luminous white structures of irregular thickness in K. blossfeldiana Clandiva Hayworth, and white Y-shaped structures appeared higher than grey ones in K. blossfeldiana Clandiva Fonda and K. blossfeldiana Clandiva Bardot, which had orange petals showing slightly zigzag structures with light gray to white in color, and irregular-shaped small structures. This research highlights the possibility of using elemental mapping and linking it with petal colour traits in plant classification.
Article Details
Article Details
Crassulaceae, Elemental mapping , Energy dispersive x-ray , Klanchoe blossfeldiana, Spectroscopy technique, Taxonomy
Al-Naib, A. I . & Al-Wattar, M. T. (2021). Molecular taxonomy and genettic diversity evaluation among seven cultivares of Klanchoe blossfeldiana Poelln using Random Amplified Polymerase DNA (RAPD) Markres . Journal of Plant Archives , 21(Suppliment-1), 294–299. https://doi.org/10.51470/plantarchives.2021.v21.s1.047
Bhardwaj,S. , Kapoor,B., Kapoor , D., Thakur, U., Dolma , U.,Y. & Raza,A. 2025. Manifold roles of potassium in mediating drought tolerance in plants and their underlying mechanisms. Journal of Plant Science V. 351. 112337 . https://doi.org/10.1016/j.plantsci.2024.11 2337
Ent, A., Przybyłowicz, W. J., Jonge, M. D., Harris, H. H., Ryan, C. G., Tylko, G., Paterson, D. J., Barnabas, A. D., Kopittke, P. M. & Mesjasz‐Przybyłowicz, J. (2018). X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants. Journal of New Phytologist, 218(2), 432–452. https://doi.org/10.1111/nph.14810
Erickson , M.F. & Pessoa , D.M. A. (2022) . Determining factors of flower coloration . Journal of Acta Botanica Brasilica, 2022, 36: e2021abb0299 . doi: 10.1590/0102-33062021abb0299
Ghobadifard, M., Farhadi, S. & Mohebbi, S. (2019). Catalytic performance of ZnFe2O4 nanoparticles prepared from the [ZnFe2O(CH3COO)6(H2O)3]·2H2O complex under microwave irradiation. Journal of Research on Chemical Intermediates, 45(2), 379–400. https://doi.org/10.1007/s1116401836076
Husted, S., Persson, D. P., Laursen, K. H., Hansen, T. H., Pedas, P., Schiller, M., & Schjoerring, J. K. (2011). The role of atomic spectrometry in plant science. Journal of Analytical Atomic Spectrometry, 26(1), 52-79. https://doi.org/10.1039/COJA00058B
Frimpong-Manso, S., Asiedu-Gyekye, I. J., Naadu, J. P., Magnus-Aryitey, G. T., Nyarko, A. K., Boamah, D. & Awan, M. (2015). Micro and Macro Element Composition of Kalanchoe integra Leaves: An Adjuvant Treatment for Hypertension in Ghana. International Journal of Hypertension, 2015, 1–5. https://doi.org/10.1155/2015/579497
Lianah , L., Idris , F. & Krisantini , K. (2019). Short Communication: Analysis of the chemical constituents and micromorphology of Bauhinia scandens using SEM-EDS techniques. Biodiversitas Journal of Biological Diversity, 20(7). https://doi.org/10.13057/biodiv/d200736
Lu,j.,Wu ,D., Li ,S . & Gao ,X. ( 2024 ) . Reaction Process of Solid Waste Composite-Based Cementitious Materials for Immobilizing and Characterizing Heavy Metals in Lead and Zinc Tailings: Based on XRD, SEM-EDS and Compressive Strength Characterization . Journal of Molecules , 29, 996. https://doi.org/10.3390/molecules29050996
Manimaran , G., Duraisamy ,S., Subramanium ,Th., Rangasamy , A., Alagarsamy , C., James , P., Selvamani e , S., Perumal a , D., Veerappan ,M., Arunan , Y.,E. & Periakaruppan , J. 2025. Silicon-driven approaches to salinity stress tolerance: Mechanisms, uptake dynamics, and microbial transformations. Journal of Plant Stress 16 . 100825 . https://doi.org/10.1016/j.stress.2025.100825
Nakanishi, T. M. (2021). Novel Plant Imaging and Analysis Water, Elements and Gas, Utilizing Radiation and Radioisotopes. In Springer eBooks (1st ed.). Springer Nature. https://doi.org/10.1007/978-981-33-4992-6
Norton, J. (2021 ). The Determination of Atomic Weights. In sites pitt. The Large Scale Structure of Inductive Inference. Large chapter Atomic weight . pdf . http://www.pitt.edu/~jdnorton.
Sánchez-Lara, F., Manzanares-Acuña, E., Badillo-Almaraz, V., Gutiérrez-Hernández, R., García-Aguirre, K. K., Vargas-Díaz, M. E., Hernández-Rangel, Á. O., Hernández-Sánchez, K. M. & Escobar-León, M. C. (2022). Comparative Study of Heavy Metals in Selected Medicinal Plants and Extracts, Using Energy Dispersive X-ray Fluorescence. Journal of Applied Sciences, 12(22), 11772. https://doi.org/10.3390/app122211772
Shaw, J. (2008). An investigation of the cultivated Kalanchoe daigremontiana group, with a checklist of Kalanchoe cultivars. Journal of Hanburyana, 3, 17–79. https://api.semanticscholar.org/CorpusID:82007589
Shitein, R., Smith, G. F. & Ikeda, J. (2021). Aspects of the taxonomy of the Kalanchoe daigremontiana species complex (Crassulaceae subfam. Kalanchooideae) and associated interspecific hybrids in southern Madagascar, with the description of a new nothospecies, K. ×descoingsii (=K. laetivirens × K. tubiflora). Journal of Phytotaxa, 524(4), 235–260. https://doi.org/10.11646/phytotaxa.524.4.2
Siroka, Z. (2023). Toxicity of House Plants to Pet Animals. Journal of Toxins, 15(5). https://doi.org/10.3390/toxins15050346
Takaku, Y., Takehara, S., Suzuki, C., Suzuki, H., Shimomura, M. & Hariyama, T. (2020). In situ elemental analyses of living biological specimens using “NanoSuit” and EDS methods in FESEM. Journal of Scientific Reports, 10(1), 14574. https://doi.org/10.1038/s415980 20715238
Trunschke, J., Lunau, K., Pyke, G. H., Ren, Z.-X. & Wang, H. ( 2021 ). Flower Color Evolution and the Evidence of Pollinator-Mediated Selection. Journal of Frontiers in Plant Science, 12, 617851. https://doi.org/10.3389/fpls.2021.617851
Wang , Y., Zhang,X., Zhang ,W., Peng,M., Tan, M., Qaseem,M.,F., Li , H.and Wu,A. ( 2023 )
Physiological and transcriptomic responses to magnesium deficiency in Neolamarckia Cadamba . Journal Plant Physiology and Biochemistry .V. 197. 107645.https://doi.org/10.1016/j.plaphy.2023.107645
Win, Z., Soe Oo, S. & Win Kyaw, N. (2020). Elemental Concentration of Jasmine Flowers and Their Uses. Journal of Engineering Education and Applied Science, 02(01), 321–324.
Xie, C., Hu, C., Deng, X., Shao, W., Gao, Y., Huang, W. & Song, X. (2023). Relationship between flower color and cellular physicochemical factors in Bletilla striata. Journal of Horticulturae, 9(4), 426. https://doi.org/10.3390/horticulturae9040426 .
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