Physiological and biochemical evaluation of Vitamin H7 in Rabbits stressed by Triton
Article Main
Abstract
Vitamin H7 (biotin) is essential for fatty acid synthesis and glucose metabolism. Under stress conditions, such as those induced by Triton (a nonionic surfactant that increases oxidative stress markers and induces hyperlipidemia), the body may require more biotin to manage oxidative stress and maintain metabolic balance. Studying its effects in Triton-stressed rabbits can reveal its potential protective benefits. The aim was to understand how Triton affects the vital functions of rabbits and whether Vitamin H7 plays a role in protecting these functions from negative impacts. Forty male rabbits were divided into four groups and treated daily for eight weeks. The control received no treatment. The Triton group was given water with Triton 300 mg/kg. The Vitamin H7 group received 200 µg/kg, and the Triton and Vitamin H7 group received both treatments. The treatment continued for eight consecutive weeks. The Triton group showed the highest activity of aryl esterase and bilirubin, as well as the lowest activity of superoxide dismutase (SOD) and acetylcholinesterase (Ach), while the Vitamin H7 group showed low activity of aryl esterase and Total Bilirubin, Along with high superoxide dismutase (SOD) activity. The Triton with Vitamin H7 group showed mitigation of the negative effect of triton and improvement in total Bilirubin levels. Triton induces toxic effects, such as oxidative stress, and impacts cell membranes, leading to an increase in aryl esterase activity and a decrease in Ach and SOD. In contrast, Vitamin H7 helps mitigate these toxic effects by supporting cellular metabolism and improving enzyme function, reducing total bilirubin levels, and enhancing liver function.
Article Details
Article Details
Keywords
Bilirubin, Enzymes, Oxidative stress, Triton , Vitamin H7
References
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Lengfeld, J., Zhang, H., Stoesz, S., Murali, R., Pass, F., Greene, M.I., Goel, P.N. & Grover, P. (2021). Challenges in detection of serum oncoprotein: relevance to breast cancer diagnostics. Breast Cancer: Targets and Therapy, 575-593. https://doi.org/10.2147/BCTT.S331844
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Mukherjee, S., Banerjee, O. & Singh, S. (2023). The role of B vitamins in protecting mitochondrial function. In Molecular Nutrition and Mitochondria, 167-193. https://doi.org/10.1016/B978-0-323-90256-4.00001-1
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Olfat, N., Ashoori, M. & Saedisomeolia, A. (2022). Riboflavin is an antioxidant: A review update. British Journal of Nutrition, 128(10), 1887-1895. https://doi.org/10.1017/S0007114521005031
Patani, A., Balram, D., Yadav, V.K., Lian, K.Y., Patel, A. & Sahoo, D.K. (2023). Harnessing the power of nutritional antioxidants against adrenal hormone imbalance-associated oxidative stress. Frontiers in Endocrinology, 14, 1271521. https://doi.org/10.3389/fendo.2023.1271521
Penberthy, W.T., Sadri, M. & Zempleni, J. (2020). Biotin. In Present Knowledge in Nutrition, 289-303. Academic Press . https://doi.org/10.1016/B978-0-323-66162-1.00017-2
Rafeeq, H., Hussain, A., Shabbir, S., Ali, S., Bilal, M., Sher, F. & Iqbal, H.M. (2022). Esterases as emerging biocatalysts: Mechanistic insights, genomic and metagenomic, immobilization, and biotechnological applications. Biotechnology and applied biochemistry, 69(5), 2176-2194. https://doi.org/10.1002/bab.2277
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Soncini, R. & Klein, W. (2023). Surface tension in biological systems-a common problem with a variety of solutions. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 111475. https://doi.org/10.1016/j.cbpa.2023.111475
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Ashok, P. (2021). Proteomic Analysis of Exosomes Produced by Human Pluripotent Stem Cells During Xeno-Free Differentiation in Stirred-Suspension Toward Cardiomyocytes Doctoral dissertation, Tufts University.
Atamanalp, M., Parlak, V., Özgeriş, F.B., Çilingir Yeltekin, A., Ucar, A., Keleş, M.S. & Alak, G. (2021). Treatment of oxidative stress, apoptosis, and DNA injury with N-acetylcysteine at simulative pesticide toxicity in fish. Toxicology Mechanisms and Methods, 31(3), 224-234. https://doi.org/10.1080/15376516.2021.1871794
Bianco, A. (2021). Meccanismi di protezione della Total Bilirubin a contro il danno metabolico a livello cellulare. https://hdl.handle.net/11368/2988914
Boraschi, D., Alijagic, A., Auguste, M., Barbero, F., Ferrari, E., Hernadi, S., Mayall, C., Michelini, S., Navarro Pacheco, N.I., Prinelli, A. & Swart, E. (2020). Addressing nanomaterial immunosafety by evaluating innate immunity across living species. Small, 16(21). https://doi.org/10.1002/smll.202000598
Chen, J., Zheng, Y., Fan, Y., Yang, J., Yang, J., Lu, Z., Cai, K., Guo, Y., Huang, J., Sun, Q. & Wang, F. (2024). Novel non-radical elimination mechanism of phosphorus flame retardant parent compound in Triton X-100 enhanced solar activated peroxydisulfate process: Key role of energy transfer from singlet oxygen to Triton X-100. Separation and Purification Technology, 335. https://doi.org/10.1016/j.seppur.2023.126148
Coremen, M., Turkyilmaz, I.B., Us, H., Us, A.S., Celik, S., Ozel, A.E., Bulan, O.K. & Yanardag, R. (2022). Lupeol inhibits pesticides induced hepatotoxicity via reducing oxidative stress and inflammatory markers in rats. Food and Chemical Toxicology, 164. https://doi.org/10.1016/j.fct.2022.113068
Creeden, J. F., Gordon, D. M., Stec, D. E., & Hinds Jr, T. D. (2021). Bilirubin as a metabolic hormone: the physiological relevance of low levels. American Journal of Physiology-Endocrinology and Metabolism, 320(2),E191-E207. https://doi.org/10.1152/ajpendo.00405.2020
Fathi, M., & Mardani, P. (2024). Stress in Broiler Farming. Modern Technology and Traditional Husbandry of Broiler Farming, 41. https://doi.org/10.5772/intechopen.1005612
Godswill, A.G., Somtochukwu, I.V., Ikechukwu, A.O. & Kate, E.C. (2020). Health benefits of micronutrients (vitamins and minerals) and their associated deficiency diseases: A systematic review. International Journal of Food Sciences, 3(1),1-32. https://doi.org/10.47604/ijf.1024
Gooran, N., Tan, S.W., Frey, S.L. & Jackman, J.A. (2024). Unraveling the Biophysical Mechanisms of How Antiviral Detergents Disrupt Supported Lipid Membranes: Toward Replacing Triton X-100. Langmuir, 40(12), 6524-6536. https://doi.org/10.1021/acs.langmuir.4c00174
Gwozdzinski, K., Pieniazek, A. & Gwozdzinski, L. (2021). Reactive oxygen species and their involvement in red blood cell damage in chronic kidney disease. Oxidative medicine and cellular longevity, 2021(1). https://doi.org/10.1155/2021/6639199
Haro-Reyes, T., Díaz-Peralta, L., Galván-Hernández, A., Rodríguez-López, A., Rodríguez-Fragoso, L. Ortega-Blake, I. (2022). Polyene antibiotics physical chemistry and their effect on lipid membranes; impacting biological processes and medical applications. Membranes, 12(7), 681. https://doi.org/10.3390/membranes12070681n
Honeder, S.E., Tomin, T., Schinagl, M., Pfleger, R., Hoehlschen, J., Darnhofer, B., Schittmayer, M. & Birner‐Gruenberger, R. (2023). Research Advances Through Activity‐Based Lipid Hydrolase Profiling. Israel Journal of Chemistry, 63(3-4). https://doi.org/10.1002/ijch.202200078
Inverarity, C. B. (2020). Development of a" Pro-Matrix" Advanced Cell Scaffold Biomaterial to Support Mesenchymal Stem Cell Therapy. Open University (United Kingdom).
Kannan, S., Balakrishnan, J. Nagarajan, P. (2024). Vitamin B7 (Biotin) and Its Role in Hair, Skin and Nail Health. In Hydrophilic Vitamins in Health and Disease, 233-252. https://doi.org/10.1007/978-3-031-55474-2_10
Lampitella, E.A., Marone, M., Achanta, N.S., Porzio, E., Trepiccione, F. & Manco, G. (2024). The Human Paraoxonase 2: An Optimized Procedure for Refolding and Stabilization Facilitates Enzyme Analyses and a Proteomics Approach. Molecules, 29(11), 2434. https://doi.org/10.3390/molecules29112434
Lengfeld, J., Zhang, H., Stoesz, S., Murali, R., Pass, F., Greene, M.I., Goel, P.N. & Grover, P. (2021). Challenges in detection of serum oncoprotein: relevance to breast cancer diagnostics. Breast Cancer: Targets and Therapy, 575-593. https://doi.org/10.2147/BCTT.S331844
Mohammadi, M.A., Farshi, P., Ahmadi, P., Ahmadi, A., Yousefi, M., Ghorbani, M. & Hosseini, S.M. (2023). Encapsulation of vitamins using nanoliposome: Recent advances and perspectives. Advanced Pharmaceutical Bulletin, 13(1),48. https://doi.org/10.34172/apb.2023.005
Mukherjee, S., Banerjee, O. & Singh, S. (2023). The role of B vitamins in protecting mitochondrial function. In Molecular Nutrition and Mitochondria, 167-193. https://doi.org/10.1016/B978-0-323-90256-4.00001-1
Nemček, L. & Hagarová, I. (2021). The recent strategies employed in chemical analysis of contaminated waters, sediments and soils as a part of the remediation process: Extraction. Environmental Pollution and Remediation, 131-173. https://doi.org/10.1007/978-981-15-5499-5_5
Ofoedu, C.E., Iwouno, J.O., Ofoedu, E.O., Ogueke, C.C., Igwe, V.S., Agunwah, I.M., Ofoedum, A.F., Chacha, J.S., Muobike, O.P., Agunbiade, A.O. & Njoku, N.E. (2021). Revisiting food-sourced vitamins for consumer diet and health needs: A perspective review, from vitamin classification, metabolic functions, absorption, utilization, to balancing nutritional requirements. PeerJ, 9, e11940. https://doi.org/10.7717/peerj.11940
Olfat, N., Ashoori, M. & Saedisomeolia, A. (2022). Riboflavin is an antioxidant: A review update. British Journal of Nutrition, 128(10), 1887-1895. https://doi.org/10.1017/S0007114521005031
Patani, A., Balram, D., Yadav, V.K., Lian, K.Y., Patel, A. & Sahoo, D.K. (2023). Harnessing the power of nutritional antioxidants against adrenal hormone imbalance-associated oxidative stress. Frontiers in Endocrinology, 14, 1271521. https://doi.org/10.3389/fendo.2023.1271521
Penberthy, W.T., Sadri, M. & Zempleni, J. (2020). Biotin. In Present Knowledge in Nutrition, 289-303. Academic Press . https://doi.org/10.1016/B978-0-323-66162-1.00017-2
Rafeeq, H., Hussain, A., Shabbir, S., Ali, S., Bilal, M., Sher, F. & Iqbal, H.M. (2022). Esterases as emerging biocatalysts: Mechanistic insights, genomic and metagenomic, immobilization, and biotechnological applications. Biotechnology and applied biochemistry, 69(5), 2176-2194. https://doi.org/10.1002/bab.2277
Rodríguez-Cano, A.M., Calzada-Mendoza, C.C., Estrada-Gutierrez, G., Mendoza-Ortega, J.A. & Perichart-Perera, O. (2020). Nutrients, mitochondrial function, and perinatal health. Nutrients, 12(7), 2166. https://doi.org/10.3390/nu12072166
Soncini, R. & Klein, W. (2023). Surface tension in biological systems-a common problem with a variety of solutions. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 111475. https://doi.org/10.1016/j.cbpa.2023.111475
Zhanassova, K., Kurmanbayeva, A., Gadilgereyeva, B., Yermukhambetova, R., Iksat, N., Amanbayeva, U., Bekturova, A., Tleukulova, Z., Omarov, R. & Masalimov, Z. (2021). ROS status and antioxidant enzyme activities in response to combined temperature and drought stresses in barley. Acta Physiologiae Plantarum, 43, 1-12. https://doi.org/10.1007/s11738-021-03281-7
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Physiological and biochemical evaluation of Vitamin H7 in Rabbits stressed by Triton. (2025). Journal of Applied and Natural Science, 17(3), 1062-1067. https://doi.org/10.31018/jans.v17i3.6650
