Genetic diversity analysis of some Coriandrum sativum cultivars exposed to laser using some molecular indicators
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Abstract
Coriander, Coriandrum sativum L. is an herbal crop from the family Apiaceae, It has a wide range of uses, including as a flavouring agent and in medical applications. Therefore, physical factors have recently been given attention, including laser radiation applications used in agriculture for biostimulating seeds and seedlings, thereby leading to changes in the DNA sequence. The present study aimed to identify genetic changes in Coriander plants exposed to laser radiation.Coriander seeds were obtained from local markets in Mosul city in different varieties (local, Syrian, Italian). The study found that there were differences at the molecular level between the three types of coriander used in the study, the difference in the total number of bands/variety of coriander for the total of the five primers used (OPA1, OPA2, OPD1 OPK, POD3) according to the difference in the period of exposure to laser radiation three different times 5 ,10,20 minutes. Depending on the results of the RAPD-PCS analysis, the total number of amplification bands was 182, comprising 180 polymorphic bands and 2 unique bands, which accounted for 98.9% and 1.10%, respectively. There were differences at the molecular level between the three types of coriandrum according to the different treatments with laser radiation and this primer. Genetic similarity was calculated, with a maximum similarity value of 0.9 and a minimum similarity value of 0.2. The cluster tree analysis showed that genotypes that are close to each other are more similar than those that are far apart. This study used diversity genetic information and different types of coriander seeds with different primers compared with other study .
Article Details
Article Details
Coriandrum sativum , RAPD-PCS, Laser radiation, Mutation
Bhat, S., Kaushal, P., Kaur, M. & Sharma, H. K. (2014). Coriander (Coriandrum sativum L.): Processing, nutritional and functional aspects. African Journal of Plant Science, 8(1), 25-33. https://doi.org/10.5897/AJPS2013.1118.
Chen, Y. P., Yue, M. & Wang, X. L. (2005). Influence of He-Ne laser irradiation on seeds thermodynamic parameters and seedlings growth of Isatis indogotica. Plant Science 168(3),601–606. https://doi.org/10.1016/j.plantsci.2004.09.005
Ezzulddin, T. A. M., Jwher, M. & Dabdoub, S. A. (2020). Detection of similarity and genetic distance between Iraqi chicken varieties and different standard strains. Iraqi Journal of Veterinary Sciences, 34(2), 333-337. DOI: 10.33899/ijvs.2019.126109.1235
Hajlaoui, H., Arraouadi, S., Noumi, E., Aouadi, K., Adnan, M., Khan, M. A., Kadri, A. & Snoussi, M. (2021). Antimicrobial, antioxidant, anti-acetylcholinesterase, antidiabetic, and pharmacokinetic properties of Carum carvi L. and Coriandrum sativum L. essential oils alone and in combination. Molecules, 26 (12), 3625. https://doi.org/10.3390/molecules26123625
Hasan, M., Hanafiah, M. M., Aeyad Taha, Z., AlHilfy, I. H., & Said, M. N. M. (2020). Laser irradiation effects at different wavelengths on phenology and yield components of pretreated maize seed. Applied Sciences, 10(3), 1189
Hassan, M., Shaaban, S. A., El Ziat, R. A., & Khaled, K. A. (2024). Laser-induced changes in the gene expression, growth and development of gladiolus grandiflorus cv.“White prosperity”. Scientific Reports, 14(1), 6257.
Ibrahim, M. A. & Faisal, R. M. (2024). Molecular characterization of antibiotic resistance and virulence genes on plasmids of Proteus mirabilis isolated from urine samples of Hospitals in Mosul City, Iraq. Journal of Applied and Natural Science, 16(2), 830-841. https://doi.org/10.31018/jans.v16i2.5526.
Lin, H. H., Lin, K. H., Yang, M. J., Nguyen, H. C., Wang, H. J., Huang, H. X., & Huang, M. Y. (2022). Physiological responses and antioxidant properties of coriander plants (Coriandrum sativum L.) under different light intensities of red and blue lights. Scientific Reports Scientific Reports, 12(1), 21139. https://doi.org/10.1038/s41598-022-25749-3.
Mahleyuddin, N. N., Moshawih, S., Ming, L. C., Zulkifly, H. H., Kifli, N., Loy, M. J., Sarker, M. M. R., Al-Worafi, Y. M., Goh, B. H., Thuraisingam, S. & Goh, H. P. (2021). Coriandrum sativum L.: A Review on Ethnopharmacology, Phytochemistry, and Cardiovascular Benefits. Molecules. 27(1), 209. https://doi.org/10.3390/molecules27010209.
Melo, R. A., Morales, R. G. F., Bucsan, E. E., Costa, J. C., Resende, L. V. & Menezes, D. (2010). Genetic diversity in coriander genotypes based on molecular marker RAPD. Horticultura Brasileira, 28(2), 207-211.
Michiels, A. N., Van den Ende, W., Tucker, M., Van Riet, L., & Van Laere, A. (2003). Extraction of high-quality genomic DNA from latex-containing plants. Analytical biochemistry, 315(1), 85-89.
Osman, Y. A., El-Tobgy, K. M. & El-Sherbini, E. S. A. (2009). Effect of laser radiation treatments on growth, yield and chemical constituents of fennel and coriander plants. J. Appl. Sci. Res, 5(3), 244-252.
Podleśny, J., Stochmal, A., Podleśna, A., & Misiak, L. E. (2012). Effect of laser light treatment on some biochemical and physiological processes in seeds and seedlings of white lupine and faba bean. Plant Growth Regulation, 67, 227–233. https://doi.org/10.1007/s10725-012-9681-7.
Roychowdhury, R. & Tah, J. (2011). Assessment of chemical mutagenic effect in mutation breeding programme for M1 generation of carnation (Dianthus caryophyllus). Research in Plant Biology 1(4), 23–32 .
Scandar, S., Zadra, C. & Marcotullio, M. C. (2023). Coriander (Coriandrum sativum) Polyphenols and Their Nutraceutical Value against Obesity and Metabolic Syndrome. Molecules. 28(10), 4187. https://doi.org/10.3390/molecules28104187.
Sharma, A., Rajpurohit, D., Jain, D.,Verma, P. & Joshi, A. (2019). Molecular characterization of coriander (Coriandrum sativum L.) genotypes using random amplified polymorphic DNA (RAPD) markers. Journal of Pharmacognosy and Phytochemistry, 8(3), 4770-4775 .
Shoaib, H., Ayyildiz, H. F., Ismail, U. & Kara, H. (2023). Antimicrobial Activity of Coriander. In Handbook of Coriander (Coriandrum sativum), 1st ed.; CRC Press: Boca Raton, FL, USA, 123–144.
Singh, S. K., Kakani, R. K., Meena, R. S., Pancholy, A., Pathak, R. & Raturi, A. (2012). Studies on genetic divergence among Indian varieties of a spice herb, Coriandrum sativum L. Journal of Environmental Biology., 33, 781-789.
Tomar-Rukam, S., Kulkarni, G. U., Parakhia, M. V., Thakkar, J. R., Rathod, V. M., Solanki, R. K. & Golakiya, B. A. (2014). Genetic diversity analysis in coriander (Coriandrum sativum) genotypes through morphological and molecular characterization. Res. J. Biotech, 9, 1-11.
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