Effect of adding different concentrations of chamomile (Matricaria chamomilla L) extract to preserve the quality of chilled Awassi ram semen
Article Main
Abstract
Chamomile extract has numerous compounds that act as antioxidants, enhancing semen characteristics during cooling for 72 h. The present study aimed to evaluate the effects of adding chamomile (Matricaria chamomilla L) extract to sperm extenders during cooling storage on semen quality parameters. Three rams were taken, semen was pooled and distributed into four parts and diluted with a Tris-based extender. Therefore, the control group was C0, without chamomile extract; the second group was C1 (0.30 mg chamomile extract); the third group was C2 (0.60 mg chamomile extract); and the fourth group was C3 (0.90 mg chamomile extract). Samples were stored at 4 °C for 72 h. The individual motility, live sperm, membrane integrity, total, and types of abnormalities (head, middle, and tail) of sperm were evaluated. Results showed that individual motility, live sperm, and sperm membrane integrity in the C1 group were significantly increased (P<0.05) throughout storage. The C2 group showed significant improvements (P<0.05) in individual motility and sperm membrane integrity for 72 h. Sperm abnormalities decreased significantly (P<0.05) in groups C1 and C2 at 48 and 72 h compared to the C0 and C3 groups. Abnormal tail sperm decreased significantly (P<0.05) in the C1, C2, and C3 groups compared to the C0 group at 72 h. The study indicated that adding 0.30 mg and then 0.60 mg of chamomile extract to semen extender enhances sperm motility, sperm membrane integrity, and live sperm, while also reducing total abnormal sperm and abnormal sperm (head, middle, and tail) compared to 0.90 mg.
Article Details
Article Details
Keywords
Antioxidants, Chamomile, Chilling, Extender, Ram semen
References
Abd Noor, S. A. A. M. (2020). Studying the Pharmaceutical; Elements and Bio-Effect of the Chamomile Plants as a Medicinal Plant. Systematic Reviews in Pharmacy, 11(6). DOI: 10.31838/srp.2020.6.108
Ahmed, H. H., El-Sallami, I. H., Abdul-Hafeez, E. Y., & Ibrahim, O. H. (2022). Response of growth, flowering, nutrient uptake and essential oil of German chamomile to organic nutrition. Assiut Journal of Agricultural Sciences, 53(5), 78-92.
Akhter, S., Zubair, M., Mahmood, M., Andrabi, S. M. H., Hameed, N., Ahmad, E., & Saleemi, M. K. (2023). Effects of vitamins C and E in tris citric acid glucose extender on chilled semen quality of Kail ram during different storage times. Scientific Reports, 13(1), 18123. https://doi.org/10.1038/s41598-023-43831-2
Akram, W., Ahmed, S., Rihan, M., Arora, S., Khalid, M., Ahmad, S., Ahmad, F., Haque, S., & Vashishth, R. (2024). An updated comprehensive review of the therapeutic properties of Chamomile (Matricaria chamomilla L.). International Journal of Food Properties, 27(1), 133-164. https://doi.org/10.1080/10942912.2023.2293661
Al-Ameri, M. H. (2022). Evaluation of testicular biometry and spermatozoa recovered after slaughter from cauda epididymal of Awassi ram. Archives of Razi Institute, 77(6), 2329-2334. DOI: 10.22092/ARI.2022.358950.2338
Al-Ameri, M. H. (2023). Effect of addition of honey and skim milk and cooled cauda epididymal spermatozoa of Awassi ram. Bionatura, 8(2):1-5. http://dx.doi.org/10.21931/RB/2023.08.02.70
Al-Snafi, A. E., & Hasham, L. F. (2023). Bioactive constituents and pharmacological importance of matricaria chamomilla: a recent review. GSC Biological and Pharmaceutical Sciences, 22(2), 079-098. . https://doi.org/10.30574/gscbps.2023.22.2.0477
Al-Subaihawi, H. R. B.& Al-Saab, H. K. J. (2017). Extractiuon and lyophilization of the low density of lipoprotein instead of egg yolk on some semen parameters of Awassi rams. The Iraqi Journal of Agricultural Sciences. 48(1): 350-359.
Al-Subaihawi, H. R. B., Al-Saab, H. K. J. & AL-Sarray, M. A. R. (2017). Extractiuon and lyophilization low density of lipoproteins and used it instead of egg yolk in semen extender of Awassi rams and their influence in sperm abnormalities store at 5 ° C. The Iraqi Journal of Agricultural Sciences. 48(1): 342-349.
Allai, L., Druart, X., Terzioğlu, P., Louanjli, N., Nasser, B., Ozturk, M., & El Amiri, B. (2023). The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk‐Based Extender Impacts Ram Sperm Quality. Veterinary Medicine International, 2023(1), 6248890. https://doi.org/10.1155/2023/6248890
Azawi, O. I., Al-Dahash, S. Y. A., and Juma, F. T. (1993). Effect of different diluents on Shami goat semen. Small Ruminant Research, 9(4), 347-352.
Bahmani, M., Saki, K., Golshahi, H., Rafieian-Kopaei, M., Abdali, N., Adineh, A., Namdari, F., & Bahmani, F. (2015). Ethnobotanical and therapeutic uses of camomille. J Chem Pharm Res, 7(1), 640-5.
Bassuony, N. I., Assi, M. M., Sakr, A. A., Bakr, A. A., Anwar, D. A., & Badr, M. R. (2023). Effect of Bioactive Compounds of Lemon, Onion and Garlic Extract on Buffalo Semen Quality, Antioxidant Status and Ultrastructure Changes. Alexandria Journal of Veterinary Sciences, 76(1): 108-116. DOI: 10.5455/ajvs.124422
Berakdar, N., & Alahmad, A. (2022).Review of oxidative stress and antioxidants. Innovare Journal of Life Sciences, 10(1):1-3.http://dx.doi.org/10.22159/ijls.2022v10i1.43577
Berean, D. I., Bogdan, L. M., & Cimpean, R. (2024). Advancements in Understanding and Enhancing Antioxidant-Mediated Sperm Cryopreservation in Small Ruminants: Challenges and Perspectives. Antioxidants, 13(6), 624. https://doi.org/10.3390/antiox13060624
Bisht, S. P. S., Mishra, R., & Kumari, K. (2012). Anti-microbial and free radical scavenging activity of chamomile flower essential oil. Asian Journal of Pharmaceutical and Health Sciences, 2(1): 283-285.
Chan, E. W. C., Lim, Y. Y., & Chew, Y. L. (2007). Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food chemistry, 102(4), 1214-1222. doi:10.1016/j.foodchem.2006.07.009
Chauhan, R., Singh, S., Kumar, V., Kumar, A., Kumari, A., Rathore, S., Kumar, R., & Singh, S. (2022). A comprehensive review on biology, genetic improvement, agro and process technology of German chamomile (Matricaria chamomilla L.). Plants, 11(1), 29. https://doi.org/10.3390/plants11010029
Chiş, M. S., Păucean, A., Man, S., Pop, A., Mureşan, A. E., Fostoc, G., & Muste, S. (2019). A comprehensive review regarding the botanical origin, medicinal uses and chemical composition of roman and German chamomile. Hop and Medicinal Plants, 1-2:28-37.
Chiselita, O., Darie, G., Rotari, D., Chiselita, N., Besliu, A., Efremova, N., & Caraman, M. (2023). Brewer yeast mannoproteins as an efficient supplement for preservation of ram sperm by refrigeration. Scientific Papers. Series D. Animal Science, 66(1).
Dai, Y. L., Li, Y., Wang, Q., Niu, F. J., Li, K. W., Wang, Y. Y., Wang, J., Zhou, C.Z.& Gao, L. N. (2023). Chamomile: a review of its traditional uses, chemical constituents, pharmacological activities and quality control studies. Molecules, 28(1), 133. https://doi.org/10.3390/molecules28010133
Diab, H. M., Ahmed, O. M., Fahim, H. I., & Mohamed, M. Y. (2021). Usage of some natural or synthetic compounds as antioxidant and their effects on cryopreservation and penetration of ram spermatozoa. Adv. Anim. Vet. Sci, 9(5), 743-753. http://dx.doi.org/10.17582/journal.aavs/2021/9.5.743.753
Dias, S. D., Andrade, L. P., Rolo, J., Delgado, F. M. G., & Oliveira, A. C. P. D. (2022). Effect of plant extracts in sperm viability. III Colóquio Nacional de Plantas Aromáticas e Medicinais, 129-135.
El Amiri, B., & Rahim, A. (2024). Exploring Endogenous and Exogenous Factors for Successful Artificial Insemination in Sheep: A Global Overview. Veterinary Sciences, 11(2), 86. https://doi.org/10.3390/vetsci11020086
EL Mihyaoui, A., Castillo, M. I. E. C., Antonio, C. A. N. O., Hernandez-Ruiz, J., Lamarti, A., & Arnao, M. B. (2021). Comparative study of wild chamomile plants from the north-west of Morocco: Bioactive components and total antioxidant activity. Journal of Medicinal Plants Research, 5(10), 431-441. DOI: 10.5897/JMPR2021.7159
El Mihyaoui, A., Esteves da Silva, J. C., Charfi, S., Candela Castillo, M. E., Lamarti, A., & Arnao, M. B. (2022). Chamomile (Matricaria chamomilla L.): a review of ethnomedicinal use, phytochemistry and pharmacological uses. Life, 12(4), 479. https://doi.org/10.3390/life12040479
Fırat, Z., Demirci, F., & Demirci, B. (2018). Antioxidant activity of chamomile essential oil and main components. Natural Volatiles and Essential Oils, 5(1), 11-16.
Gundogan, M. 2009. Short term preservation of ram semen with different extenders. Kafkas Univ Vet Fak Derg. 15(3):429-435. DOI:10.9775/kvfd.2009.033-A
Ifeanyi, O. E. (2018). A review on free radicals and antioxidants. Int. J. Curr. Res. Med. Sci, 4(2), 123-133. http://dx.doi.org/10.22192/ijcrms.2018.04.02.019
Jeyendran, R. S., Van der Ven, H. H., Perez-Pelaez, M., Crabo, B. G., and Zaneveld, L. J. D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Reproduction, 70(1): 219-228.
Jumintono, J., Alkubaisy, S., Singh, K., Mikolaychik, I., & Morozova, L. (2021). Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 C for 50 hours. Archives of Razi Institute, 76(4), 115. DOI: 10.22092/ari.2021.355901.1735
Khodadadi, H., Khodaei-Motlagh, M., Kazemibonchenari, M., & Momeni, H. R. (2023). The effect of Eggplant peel extract addition (Solanummelongena) on Farahani ram sperm after oxidative stress (freeze-thawing). Iranian Veterinary Journal, 19(1), 45-51. http://creativecommons.org/licenses/by-nc/4.0/
Khozein, S., Eslami, M., & Farrokhi-Ardabili, F. (2024). Improvement of ram semen quality by luteolin enrichment during cold preservation. Archives Animal Breeding, 67(1), 123-132. https://doi.org/10.5194/aab-67-123-2024
Lee, S. Y., Ferdinand, V., & Siow, L. F. (2022). Effect of drying methods on yield, physicochemical properties, and total polyphenol content of chamomile extract powder. Frontiers in Pharmacology, 13, 1003209. DOI 10.3389/fphar.2022.1003209
Levano, G., Quispe, J., Vargas, D., García, M., López, A., Aguila, L., & Valdivia, M. (2023). Effect of atomized black maca (Lepidium meyenii) supplementation in the cryopreservation of alpaca (Vicugna pacos) epididymal spermatozoa. Animals, 13(13), 2054. https://doi.org/10.3390/ani13132054
Loizzo, M. R., & Silva, A. S. (2021). Natural antioxidants: innovative extraction and application in foods. Foods, 10(5), 937. https://doi.org/10.3390/foods10050937
Mahdy, T., Aboufandoud, E. I. D., Ibrahem, A. L., El Badawy, A. A., El-Hawary, A. F. A., & Khalifa, E. I. (2024). Improvement of chilling spermatozoa characteristics and their fertility by supplementation of L-Arginine to ram semen extender. Egyptian Journal of Animal Production, 61(1), 13-20.
Neila-Montero, M., Alvarez, M., Riesco, M. F., Soriano-Úbeda, C., Montes-Garrido, R., Palacin-Martinez, C., de Paz, P., Anel, L., & Anel-Lopez, L. (2024). The Adaptation Time to the Extender as a Crucial Step for an Accurate Evaluation of Ram Sperm Quality during the Liquid Storage. Veterinary Sciences, 11(3), 132. https://doi.org/10.3390/vetsci11030132
Qasem, A., Assaggaf, H., Montesano, D., Khalil, Z., Al-Mijalli, S. H., Baaboua, A. E., El Omari, N., El Menyiy, N., Bakrim, S., Sheikh, R.A., Alshahrani, M.M., Al Awadh, A.A., Zengin, G., Bouyahya, A., & Mrabti, H. N. (2022). Determination of chemical compounds and investigation of biological properties of matricaria chamomilla essential oils, honey, and their mixture. Molecules, 27(18), 5850. https://doi.org/10.3390/molecules27185850
Ros-Santaella, J. L., & Pintus, E. (2021). Plant extracts as alternative additives for sperm preservation. Antioxidants, 10(5), 772. https://doi.org/10.3390/antiox10050772
Saeedi, M., Khanavi, M., & Shahsavari, K. (2024). Matricaria chamomilla: an updated review on biological activities of the plant and constituents. Research Journal of Pharmacognosy, 11(1), 109-136. DOI: 10.22127/RJP.2023.404256.2145
Sah, A., Naseef, P. P., Kuruniyan, M. S., Jain, G. K., Zakir, F., & Aggarwal, G. (2022). A comprehensive study of therapeutic applications of chamomile. Pharm aceuticals, 15(10),1284.https://doi.org/10.33 90/ph15101284
Sarma, N., Gogoi, R., Begum, T., Lal, M., Perveen, K., Alsahikh, N. A., & Alsulami, J. A. (2023). A study on the chemical profile of cultivated chamomile (Matricaria chamomilla L.) flower essential oil from North East India with special emphasis on its pharmacological importance. Journal of Essential Oil Bearing Plants, 26(3), 745-760. DOI: 10.1080/0972060X.2023.2239289
Shokry, D. M., Abd Eldaim, M. A., Badr, M. R., Khalifa, H. K., Orabi, S. H., Hassan, A. M., & Dohreig, R. (2021). Enhancement impact of Moringa oleifera leaves extract–base extender on cryopreservation and fertilization of Barki ram sperms: comparative study with vitamin E and selenium combination. Italian Journal of Animal Science, 20(1), 1175-1186. https://doi.org/10.1080/182 8051X.2021.1953411
Sohail, T., Zhang, L., Wang, X., Jiang, C., Wang, J., Sun, X., & Li, Y. (2024). Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage. Animals, 14(2), 319. https://doi.org/10.3390/ani14020319
Soltanpour, F., & Moghaddam, G. (2013). Effects of frozen diluents on storage of ram sperm. International journal of Advanced Biological and Biomedical Research, 1(12), 1698-1704.
Soltanpour, F., Moghaddam, G., Asadpour, R., & Rafat, S. A. (2014). Effect of Antioxidant combinations on sperm quality of cross breed rams during liquid storage. Int J Adv Biol Biom Res, 2(3): 732-740.
Susilowati, S., Triana, I. N., Wurlina, W., Arimbi, A., Srianto, P., & Mustofa, I. (2019). Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days. Veterinary World, 12(11), 1784.
Susilowati, S., Mustofa, I., Wurlina, W., Hernawati, T., Oktanella, Y., Soeharsono, S., & Purwanto, D. A. (2022). Green tea extract in the extender improved the post-thawed semen quality and decreased amino acid mutation of Kacang buck sperm. Veterinary Sciences, 9(8), 403. https://doi.org/10.3390/vetsci9080403
Thuwaini, M.M. (2023). Medicinal plants with antioxidant activity – A review. J Pharm Adv Res, 6(11): 1980-2016.
Triyaningrum, T., Fahrudin, M., Karja, N. W. K., & Prasetyaningtyas, W. E. (2024). The quality of chilled ram semen after storage in extender with addition of melatonin at 4° C. Jurnal Kedokteran Hewan-Indonesian Journal of VeterinarySciences, 18(1):34-39. https://doi.org/10.21157/j.ked.hewan.v18i1.34649
Vahedi, V., Hedayat Evrigh, N., Behroozlak, M., & Dirandeh, E. (2018). Antioxidant effects of Thyme (Thymus vulgaris) extract on ram sperm quality during cryopreservation. Iranian Journal of Applied Animal Science, 8(2), 263-269.
Yang, J., Zhang, X., Hua, Z., Jia, H., Li, K., & Ling, C. (2024). High-Quality Assembly and Analysis of the Complete Mitogenomes of German Chamomile (Matricaria recutita) and Roman Chamomile (Chamaemelum nobile). Genes, 15(3), 301. https://doi.org/10.3390/genes1 5030301
Ahmed, H. H., El-Sallami, I. H., Abdul-Hafeez, E. Y., & Ibrahim, O. H. (2022). Response of growth, flowering, nutrient uptake and essential oil of German chamomile to organic nutrition. Assiut Journal of Agricultural Sciences, 53(5), 78-92.
Akhter, S., Zubair, M., Mahmood, M., Andrabi, S. M. H., Hameed, N., Ahmad, E., & Saleemi, M. K. (2023). Effects of vitamins C and E in tris citric acid glucose extender on chilled semen quality of Kail ram during different storage times. Scientific Reports, 13(1), 18123. https://doi.org/10.1038/s41598-023-43831-2
Akram, W., Ahmed, S., Rihan, M., Arora, S., Khalid, M., Ahmad, S., Ahmad, F., Haque, S., & Vashishth, R. (2024). An updated comprehensive review of the therapeutic properties of Chamomile (Matricaria chamomilla L.). International Journal of Food Properties, 27(1), 133-164. https://doi.org/10.1080/10942912.2023.2293661
Al-Ameri, M. H. (2022). Evaluation of testicular biometry and spermatozoa recovered after slaughter from cauda epididymal of Awassi ram. Archives of Razi Institute, 77(6), 2329-2334. DOI: 10.22092/ARI.2022.358950.2338
Al-Ameri, M. H. (2023). Effect of addition of honey and skim milk and cooled cauda epididymal spermatozoa of Awassi ram. Bionatura, 8(2):1-5. http://dx.doi.org/10.21931/RB/2023.08.02.70
Al-Snafi, A. E., & Hasham, L. F. (2023). Bioactive constituents and pharmacological importance of matricaria chamomilla: a recent review. GSC Biological and Pharmaceutical Sciences, 22(2), 079-098. . https://doi.org/10.30574/gscbps.2023.22.2.0477
Al-Subaihawi, H. R. B.& Al-Saab, H. K. J. (2017). Extractiuon and lyophilization of the low density of lipoprotein instead of egg yolk on some semen parameters of Awassi rams. The Iraqi Journal of Agricultural Sciences. 48(1): 350-359.
Al-Subaihawi, H. R. B., Al-Saab, H. K. J. & AL-Sarray, M. A. R. (2017). Extractiuon and lyophilization low density of lipoproteins and used it instead of egg yolk in semen extender of Awassi rams and their influence in sperm abnormalities store at 5 ° C. The Iraqi Journal of Agricultural Sciences. 48(1): 342-349.
Allai, L., Druart, X., Terzioğlu, P., Louanjli, N., Nasser, B., Ozturk, M., & El Amiri, B. (2023). The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk‐Based Extender Impacts Ram Sperm Quality. Veterinary Medicine International, 2023(1), 6248890. https://doi.org/10.1155/2023/6248890
Azawi, O. I., Al-Dahash, S. Y. A., and Juma, F. T. (1993). Effect of different diluents on Shami goat semen. Small Ruminant Research, 9(4), 347-352.
Bahmani, M., Saki, K., Golshahi, H., Rafieian-Kopaei, M., Abdali, N., Adineh, A., Namdari, F., & Bahmani, F. (2015). Ethnobotanical and therapeutic uses of camomille. J Chem Pharm Res, 7(1), 640-5.
Bassuony, N. I., Assi, M. M., Sakr, A. A., Bakr, A. A., Anwar, D. A., & Badr, M. R. (2023). Effect of Bioactive Compounds of Lemon, Onion and Garlic Extract on Buffalo Semen Quality, Antioxidant Status and Ultrastructure Changes. Alexandria Journal of Veterinary Sciences, 76(1): 108-116. DOI: 10.5455/ajvs.124422
Berakdar, N., & Alahmad, A. (2022).Review of oxidative stress and antioxidants. Innovare Journal of Life Sciences, 10(1):1-3.http://dx.doi.org/10.22159/ijls.2022v10i1.43577
Berean, D. I., Bogdan, L. M., & Cimpean, R. (2024). Advancements in Understanding and Enhancing Antioxidant-Mediated Sperm Cryopreservation in Small Ruminants: Challenges and Perspectives. Antioxidants, 13(6), 624. https://doi.org/10.3390/antiox13060624
Bisht, S. P. S., Mishra, R., & Kumari, K. (2012). Anti-microbial and free radical scavenging activity of chamomile flower essential oil. Asian Journal of Pharmaceutical and Health Sciences, 2(1): 283-285.
Chan, E. W. C., Lim, Y. Y., & Chew, Y. L. (2007). Antioxidant activity of Camellia sinensis leaves and tea from a lowland plantation in Malaysia. Food chemistry, 102(4), 1214-1222. doi:10.1016/j.foodchem.2006.07.009
Chauhan, R., Singh, S., Kumar, V., Kumar, A., Kumari, A., Rathore, S., Kumar, R., & Singh, S. (2022). A comprehensive review on biology, genetic improvement, agro and process technology of German chamomile (Matricaria chamomilla L.). Plants, 11(1), 29. https://doi.org/10.3390/plants11010029
Chiş, M. S., Păucean, A., Man, S., Pop, A., Mureşan, A. E., Fostoc, G., & Muste, S. (2019). A comprehensive review regarding the botanical origin, medicinal uses and chemical composition of roman and German chamomile. Hop and Medicinal Plants, 1-2:28-37.
Chiselita, O., Darie, G., Rotari, D., Chiselita, N., Besliu, A., Efremova, N., & Caraman, M. (2023). Brewer yeast mannoproteins as an efficient supplement for preservation of ram sperm by refrigeration. Scientific Papers. Series D. Animal Science, 66(1).
Dai, Y. L., Li, Y., Wang, Q., Niu, F. J., Li, K. W., Wang, Y. Y., Wang, J., Zhou, C.Z.& Gao, L. N. (2023). Chamomile: a review of its traditional uses, chemical constituents, pharmacological activities and quality control studies. Molecules, 28(1), 133. https://doi.org/10.3390/molecules28010133
Diab, H. M., Ahmed, O. M., Fahim, H. I., & Mohamed, M. Y. (2021). Usage of some natural or synthetic compounds as antioxidant and their effects on cryopreservation and penetration of ram spermatozoa. Adv. Anim. Vet. Sci, 9(5), 743-753. http://dx.doi.org/10.17582/journal.aavs/2021/9.5.743.753
Dias, S. D., Andrade, L. P., Rolo, J., Delgado, F. M. G., & Oliveira, A. C. P. D. (2022). Effect of plant extracts in sperm viability. III Colóquio Nacional de Plantas Aromáticas e Medicinais, 129-135.
El Amiri, B., & Rahim, A. (2024). Exploring Endogenous and Exogenous Factors for Successful Artificial Insemination in Sheep: A Global Overview. Veterinary Sciences, 11(2), 86. https://doi.org/10.3390/vetsci11020086
EL Mihyaoui, A., Castillo, M. I. E. C., Antonio, C. A. N. O., Hernandez-Ruiz, J., Lamarti, A., & Arnao, M. B. (2021). Comparative study of wild chamomile plants from the north-west of Morocco: Bioactive components and total antioxidant activity. Journal of Medicinal Plants Research, 5(10), 431-441. DOI: 10.5897/JMPR2021.7159
El Mihyaoui, A., Esteves da Silva, J. C., Charfi, S., Candela Castillo, M. E., Lamarti, A., & Arnao, M. B. (2022). Chamomile (Matricaria chamomilla L.): a review of ethnomedicinal use, phytochemistry and pharmacological uses. Life, 12(4), 479. https://doi.org/10.3390/life12040479
Fırat, Z., Demirci, F., & Demirci, B. (2018). Antioxidant activity of chamomile essential oil and main components. Natural Volatiles and Essential Oils, 5(1), 11-16.
Gundogan, M. 2009. Short term preservation of ram semen with different extenders. Kafkas Univ Vet Fak Derg. 15(3):429-435. DOI:10.9775/kvfd.2009.033-A
Ifeanyi, O. E. (2018). A review on free radicals and antioxidants. Int. J. Curr. Res. Med. Sci, 4(2), 123-133. http://dx.doi.org/10.22192/ijcrms.2018.04.02.019
Jeyendran, R. S., Van der Ven, H. H., Perez-Pelaez, M., Crabo, B. G., and Zaneveld, L. J. D. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Reproduction, 70(1): 219-228.
Jumintono, J., Alkubaisy, S., Singh, K., Mikolaychik, I., & Morozova, L. (2021). Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 C for 50 hours. Archives of Razi Institute, 76(4), 115. DOI: 10.22092/ari.2021.355901.1735
Khodadadi, H., Khodaei-Motlagh, M., Kazemibonchenari, M., & Momeni, H. R. (2023). The effect of Eggplant peel extract addition (Solanummelongena) on Farahani ram sperm after oxidative stress (freeze-thawing). Iranian Veterinary Journal, 19(1), 45-51. http://creativecommons.org/licenses/by-nc/4.0/
Khozein, S., Eslami, M., & Farrokhi-Ardabili, F. (2024). Improvement of ram semen quality by luteolin enrichment during cold preservation. Archives Animal Breeding, 67(1), 123-132. https://doi.org/10.5194/aab-67-123-2024
Lee, S. Y., Ferdinand, V., & Siow, L. F. (2022). Effect of drying methods on yield, physicochemical properties, and total polyphenol content of chamomile extract powder. Frontiers in Pharmacology, 13, 1003209. DOI 10.3389/fphar.2022.1003209
Levano, G., Quispe, J., Vargas, D., García, M., López, A., Aguila, L., & Valdivia, M. (2023). Effect of atomized black maca (Lepidium meyenii) supplementation in the cryopreservation of alpaca (Vicugna pacos) epididymal spermatozoa. Animals, 13(13), 2054. https://doi.org/10.3390/ani13132054
Loizzo, M. R., & Silva, A. S. (2021). Natural antioxidants: innovative extraction and application in foods. Foods, 10(5), 937. https://doi.org/10.3390/foods10050937
Mahdy, T., Aboufandoud, E. I. D., Ibrahem, A. L., El Badawy, A. A., El-Hawary, A. F. A., & Khalifa, E. I. (2024). Improvement of chilling spermatozoa characteristics and their fertility by supplementation of L-Arginine to ram semen extender. Egyptian Journal of Animal Production, 61(1), 13-20.
Neila-Montero, M., Alvarez, M., Riesco, M. F., Soriano-Úbeda, C., Montes-Garrido, R., Palacin-Martinez, C., de Paz, P., Anel, L., & Anel-Lopez, L. (2024). The Adaptation Time to the Extender as a Crucial Step for an Accurate Evaluation of Ram Sperm Quality during the Liquid Storage. Veterinary Sciences, 11(3), 132. https://doi.org/10.3390/vetsci11030132
Qasem, A., Assaggaf, H., Montesano, D., Khalil, Z., Al-Mijalli, S. H., Baaboua, A. E., El Omari, N., El Menyiy, N., Bakrim, S., Sheikh, R.A., Alshahrani, M.M., Al Awadh, A.A., Zengin, G., Bouyahya, A., & Mrabti, H. N. (2022). Determination of chemical compounds and investigation of biological properties of matricaria chamomilla essential oils, honey, and their mixture. Molecules, 27(18), 5850. https://doi.org/10.3390/molecules27185850
Ros-Santaella, J. L., & Pintus, E. (2021). Plant extracts as alternative additives for sperm preservation. Antioxidants, 10(5), 772. https://doi.org/10.3390/antiox10050772
Saeedi, M., Khanavi, M., & Shahsavari, K. (2024). Matricaria chamomilla: an updated review on biological activities of the plant and constituents. Research Journal of Pharmacognosy, 11(1), 109-136. DOI: 10.22127/RJP.2023.404256.2145
Sah, A., Naseef, P. P., Kuruniyan, M. S., Jain, G. K., Zakir, F., & Aggarwal, G. (2022). A comprehensive study of therapeutic applications of chamomile. Pharm aceuticals, 15(10),1284.https://doi.org/10.33 90/ph15101284
Sarma, N., Gogoi, R., Begum, T., Lal, M., Perveen, K., Alsahikh, N. A., & Alsulami, J. A. (2023). A study on the chemical profile of cultivated chamomile (Matricaria chamomilla L.) flower essential oil from North East India with special emphasis on its pharmacological importance. Journal of Essential Oil Bearing Plants, 26(3), 745-760. DOI: 10.1080/0972060X.2023.2239289
Shokry, D. M., Abd Eldaim, M. A., Badr, M. R., Khalifa, H. K., Orabi, S. H., Hassan, A. M., & Dohreig, R. (2021). Enhancement impact of Moringa oleifera leaves extract–base extender on cryopreservation and fertilization of Barki ram sperms: comparative study with vitamin E and selenium combination. Italian Journal of Animal Science, 20(1), 1175-1186. https://doi.org/10.1080/182 8051X.2021.1953411
Sohail, T., Zhang, L., Wang, X., Jiang, C., Wang, J., Sun, X., & Li, Y. (2024). Astaxanthin Improved the Quality of Hu Ram Semen by Increasing the Antioxidant Capacity and Mitochondrial Potential and Mitigating Free Radicals-Induced Oxidative Damage. Animals, 14(2), 319. https://doi.org/10.3390/ani14020319
Soltanpour, F., & Moghaddam, G. (2013). Effects of frozen diluents on storage of ram sperm. International journal of Advanced Biological and Biomedical Research, 1(12), 1698-1704.
Soltanpour, F., Moghaddam, G., Asadpour, R., & Rafat, S. A. (2014). Effect of Antioxidant combinations on sperm quality of cross breed rams during liquid storage. Int J Adv Biol Biom Res, 2(3): 732-740.
Susilowati, S., Triana, I. N., Wurlina, W., Arimbi, A., Srianto, P., & Mustofa, I. (2019). Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days. Veterinary World, 12(11), 1784.
Susilowati, S., Mustofa, I., Wurlina, W., Hernawati, T., Oktanella, Y., Soeharsono, S., & Purwanto, D. A. (2022). Green tea extract in the extender improved the post-thawed semen quality and decreased amino acid mutation of Kacang buck sperm. Veterinary Sciences, 9(8), 403. https://doi.org/10.3390/vetsci9080403
Thuwaini, M.M. (2023). Medicinal plants with antioxidant activity – A review. J Pharm Adv Res, 6(11): 1980-2016.
Triyaningrum, T., Fahrudin, M., Karja, N. W. K., & Prasetyaningtyas, W. E. (2024). The quality of chilled ram semen after storage in extender with addition of melatonin at 4° C. Jurnal Kedokteran Hewan-Indonesian Journal of VeterinarySciences, 18(1):34-39. https://doi.org/10.21157/j.ked.hewan.v18i1.34649
Vahedi, V., Hedayat Evrigh, N., Behroozlak, M., & Dirandeh, E. (2018). Antioxidant effects of Thyme (Thymus vulgaris) extract on ram sperm quality during cryopreservation. Iranian Journal of Applied Animal Science, 8(2), 263-269.
Yang, J., Zhang, X., Hua, Z., Jia, H., Li, K., & Ling, C. (2024). High-Quality Assembly and Analysis of the Complete Mitogenomes of German Chamomile (Matricaria recutita) and Roman Chamomile (Chamaemelum nobile). Genes, 15(3), 301. https://doi.org/10.3390/genes1 5030301
Issue
Section
Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
How to Cite
Effect of adding different concentrations of chamomile (Matricaria chamomilla L) extract to preserve the quality of chilled Awassi ram semen. (2025). Journal of Applied and Natural Science, 17(3), 1003-1010. https://doi.org/10.31018/jans.v17i3.6479
