Deepali Singh Karuna Vishunavat


Burkholderia glumae is a seed-borne rice bacterium that causes bacterial panicle blight (BPB), which is a promising disease in many rice-growing areas around the world. The bacterium has been successfully grown on King’s B agar medium (KBM) at 280C for 48 hrs. It is Gram negative, non-spore-forming, rod-shaped with cell size 0.5 to 0.7 - 1.5 to 2.0 μm with rounded ends. The colony appears as grayish white or yellow due to the pigment. The bacterium gave positive test in gelatin liquefaction, KOH solubility and nitrate reduction and while negative test in starch hydrolysis, oxidase reaction, levan production and arginine dihydrolase test. The bacterium utilized different carbon sources viz., sugars, amino acids, sugar alcohols and organic acids when examined through BiologTMGN2 Microplate System. This study would help in control and management of seed-borne bacterial plant pathogen B. glumae.


Download data is not yet available.


Metrics Loading ...




Biochemical, Burkholderia glumae, Cultural, Morphological

Buchanan RE, Gribbons NE (1974). Bergey’sManual of Determinative Bacteriology (8thedition).Williams & Wilkins Co. Baltimore USA.
Coenye, T. and Vandamme, P. 2003. Diversity and significance of Burkholderia species occupying diverse ecological niches.Environmental Microbiology.5(9): 719-729.
Cottyn, B.; Cerez, M.T.; VanOutryve, M.F.;Barroga, J.; Swings, J. and Mew, T.W.1996. Bacterial diseases of rice. I. Pathogenic bacteria associated with sheath rot complex and grain discoloration of rice in the Philippines. Plant Dis. 80 (4): 429-437.
Goldstein, J.I.; Newbury, D.E.; Echlin, P.; Joy, D.C.; Fiori, C. and Lifshin, E. 1992. Scanning electron microscopy and X-ray microanalysis; a text for biologists, materials scientists, and geologists. Plenum Press, New York.
Jones, J.B. 1993. Evaluation of the Biolog GN MicroPlate system for identification of some plant-pathogenic bacteria.Plant Disease. 77(6): 553-558.
Palleroni, N.J. (1984). Genus I. Pseudomonas Migula 1984, 237AL.In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 141–199.Edited by N.R. Krieg & J.G. Holt. Baltimore: Williams & Wilkins.
Stolpe and Godkeri. (1981). Non -pathogenic members of genus pseudomonas in the prokaryotes. Ed.Marthineret. al., Springer Verlag, New York, pp. 719 -741.
Tsushima, S.; Naito, H. and Koitabashi, M. (1995). Change in panicle susceptibility associated with flowering rate of spikelets in bacterial grain rot of rice caused by Pseudomonas glumae. Annals of the Phytopath.Soc. of Jap. 61 (2): 109-113.
Tsushima, S.; Wakimoto, S. and Mogi, S. (1986). Selective medium for detecting Pseudomonas glumae Jurita et Tabei, the causal bacterium of grain rot of rice. Ann. Phytopath. Soc. Japn. 52: 253-259.
Urakami, T.; Ito-yoshida, C.; Araki, H.; Kijima, T.; Suzuki, K.I. and Komagata, K. (1994). Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Bu r kho ld e r ia spp . and d e s c r ipt io n o f Burkholderiavandii sp. nov. International Journal of Systematic Bacteriology 44:235-245.
Citation Format
How to Cite
Singh, D., & Vishunavat, K. (2015). Identification of a seed-borne rice bacterium, Burkholderia glumae using cultural, morphological and biochemical methods. Journal of Applied and Natural Science, 7(2), 562-566. https://doi.org/10.31018/jans.v7i2.645
More Citation Formats:
Research Articles