Detection and separation of fatty acids and proteins in monogenean flatworm Polystoma integerrimum
Article Main
Abstract
Metabolite methodology in parasites assists in gaining a better comprehension of their roles in infection, adaptation, pathogenesis, taxonomy, diagnosis, and host-parasite interactions. The present study aimed to isolate and detect fatty acids and proteins in tissue extract of the whole flatworm Polystoma integerrimum that settle in the urinary bladder of the frog Bufo viridis, using Reversed-phase high-performance liquid chromatography (RP-HPLC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) techniques, respectively.The results showed that the number of fatty acids in flatworm extract was ten. Six of them were three unsaturated fatty acids, which included oleic (C18:1n-9), linoleic (C18:2n-6), and linolenic (C18:3n-3) acids, and three other saturated fatty acids, which included myristic (C14:0), palmitic (C16:0) and stearic acids (C18:0). The palmitic (49.022%) and stearic (35.499%) fatty acids were the highest among the ten total fatty acids. In addition, the SDS-PAGE technique showed that the number of protein bands in the flatworm extract was six, where the second (76859 D), fourth (23356 D), and fifth (13966 D) bands of the extract matched the first (bovine serum albumin), fourth (pepsin), and fifth (alpha-lactalbumin) bands of the standard proteins, respectively. The other three bands, the first, third, and sixth, were not matched by any of the standard protein bands. The present study could be a starting point for studying more metabolites and increasing the present-day knowledge of their types, mechanism of action, and their role in the biological and taxonomical fields to fill the gap in data about this flatworm.
Article Details
Article Details
Keywords
Flatworm, Metabolites, RP-HPLC, Separation techniques, SDS-PAGE
References
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Gazzinelli-Guimaraes, P. H., & Nutman, T. B. (2018). Helminth parasites and immune regulation. F1000Research, 7, 1685. doi.org/10.12688/f1000research.15596.1
Grano, M. A. (2020). The Asian market of frogs as food for humans during COVID-19. Risk and consequences for public health. Medicine Papers, 6(4), 77-87. URL: https://www.researchgate.net/publication/348296156
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Hade, B. F., Al-Biatee, S. T., & Al-Rubaie, H. M. (2022). Traditional and molecular diagnosis of Haemonchus contortus in sheep in Babylon province, Iraq. Iraqi Journal of Veterinary Sciences, 36(2), 479-481. doi.org/10.33899/ijvs.2021.130533.1842
Hajji, T., Telahigue, K., Rabeh, I., & El Cafsi, M. (2022). Lipid classes and fatty acid composition in two parasitic copepods Peroderma cylindricum and Lernaeocera lusci and their respective fish hosts Sardina pilchardus and Merluccius merluccius from the Tunisian waters. Grasas y Aceites, 73(3), e469-e481. doi.org/10.3989/gya.0100211
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Leeming, S. J., Hahn, C., Koblmüller, S., McAllister, C. T., Vanhove, M. P., & Kmentová, N. (2023). Amended diagnosis, mitochondrial genome, and phylogenetic position of Sphyranura euryceae (Neodermata, Monogenea, Polystomatidae), a parasite of the Oklahoma salamander. Parasite, 30, 27-45. doi.org/10.1051/parasite/2023025
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Detection and separation of fatty acids and proteins in monogenean flatworm Polystoma integerrimum . (2025). Journal of Applied and Natural Science, 17(2), 638-647. https://doi.org/10.31018/jans.v17i2.6415
