Molecular identification and proximate composition of wild edible wood growing mushrooms of Mizoram, North East India for food safety and livelihood
Article Main
Abstract
Exploring the nutritional attributes of wild edible mushrooms can be vital in addressing global food security. However, there is a dearth of studies on wild edible mushrooms regarding their identification, nutritional status, and molecular characterization in Mizoram, North East India, an integral landscape of the Indo-Burma hotspot region. In the present study, samples of wild edible wood-growing mushrooms were collected from the Mamit and Champhai District of Mizoram. The present study investigated the identities and phylogenetic relationships of the eight fungal species using molecular approaches. The mushrooms were identified up to the species level based on fungal sequences with known identities in GenBank, viz. Auricularia delicata, Laetiporus sulphureus, Panus roseus, Lentinus squarrosulus, Pleurotus cystidiosus, Pleurotus djamor, Pleurotus pulmonarius and Schizophyllum commune. Further, the identified mushrooms were analysed for macro and micro-nutritional values to assess their suitability for human dietary intake. Results revealed that the collected wild edible mushrooms were rich sources of protein (17.79 - 36.46 g/100 g), carbohydrates (58.14 - 33.77 g/100 g) and fibres (5.63 - 8.32 g/100 g), while the amount of fat (1.93 - 2.77 g/100 g) was low. In addition to bio-molecules, the mushroom samples contained appreciable amounts of essential minerals. Therefore, the selected mushrooms can be used as a potential foodstuff and may also be used as a supplementary protein diet to maintain human health and help achieve food security and sustainable development goals. The present results encourage bio-prospecting, and the need to explore wild edible mushrooms further, especially in the least explored global biodiversity hotspots for sustainable livelihood.
Article Details
Article Details
Epixylic, Food, Molecular identification, Nutrients, Wild edible mushrooms
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410. https://doi.org/10.1016/s0022-2836(05)80360-2.
Ao, T. & Deb, C. R. (2019). Nutritional and antioxidant potential of some wild edible mushrooms of Nagaland, India. Journal of Food Science and Technology, 56(2), 1084–1089. https://doi.org/10.1007/s13197-018-03557-w.
AOAC. (2000). Official methods of analysis. 17th edn. Methods 925.10, 65.17, 974.24, 992.16. Gaithersburg, MD: The Association of Official Analytical Chemists.
Atri, N. S., Sharma, S. K., Joshi, R., Gulati, A. & Gulati, A. (2013). Nutritional and Neutraceutical composition of five wild culinary-medicinal species of genus Pleurotus (Higher Basidiomycetes) from Northwest India. International Journal of Medicinal Mushrooms, 15(1), 49–56. https://doi.org/10.1615/intjmedmushr.v15.i1.60.
Bandara, A., Rapior, S., Mortimer, P. E., Kakumyan, P., Hyde, K. D. & Xu, J. (2019). A review of the polysaccharide, protein and selected nutrient content of Auricularia, and their potential pharmacological value. Mycosphere, 10(1), 579–607. https://doi.org/10.5943/mycosphere/10/1/10.
Bernas, E., Jaworska, G. & Lisiewska, Z. (2006). Edible mushrooms as a source of valuable nutritive constituents. Acta Scientiarum Polonorum, Technologia Alimentaria, 5(1); 5-20.
Crisan, E. V. & Sands, A. (1978) “Nutritional Value of Edible Mushroom,” In: Chang, S.T. and Hayer, W. A. Eds., Biology and Cultivation of Edible Mushrooms, Academic Press, New York, pp. 137-168..
Dawadi, E., Magar, P. B., Bhandari, S., Subedi, S., Shrestha, S. & Shrestha J. (2022). Nutritional and post-harvest quality preservation of mushrooms: A review. Heliyon, 5;8(12):e12093. https://doi.org/10.1016/j.heliyon.e12093.
El-Ramady, H., Abdalla, N., Badgar, K., Llanaj, X., Törős, G., Hajdú, P., Eid, Y. & Prokisch, J. (2022). Edible mushrooms for sustainable and healthy human food: Nutritional and Medicinal Attributes. Sustainability, 14(9):4941. https://doi.org/10.3390/su14094941.
Gençcelep, H., Uzun, Y., Tunçtürk, Y., & Demirel, K. (2009). Determination of mineral contens of wild-grown edible mushrooms, Food Chemistry, 113(4): 1033-1036. https://doi.org/10.1016/j.foodchem.2008.08.058.
Gunasekara, N. W., Nanayakkara, C. M., Karunarathna, S. C. & Wijesundera, R. L. C. (2021). Nutritional aspects of three Termitomyces and four other wild edible mushroom species from Sri Lanka. Chiang Mai Journal of Science, 48, 1236–1246.
Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl.Acids.Symp. Ser. 41:95-98.
Hyde, K.D., Xu, J., Rapior, S., Jeewon, R., Lumyong, S., Niego, A. G. T., Abeywickrama, P.D., Aluthmuhandiram, J.V.S., Brahamanage, R.S., Brooks, S., Chaiyasen, A., Chethana, K.W.T., Chomnunti, P., Chepkirui, C., Chuankid, B., de Silva, N.I., Doilom, M., Faulds, C., Gentekaki, E., Stadler, M. (2019). The amazing potential of fungi: 50 ways we canexploit fungi industrially. Fungal Diversity, 97(1), 1–136. https://doi.org/10.1007/s13225-019-00430-9
Chang, S. T. & Buswell, J. A. (1996).Mushroom Nutriceuticals. World Journal of Microbiology and Biotechnology, 12, 473-476. https://doi.org/10.1007/BF00419460.
Chawngthu, Z., Vabeikhokhei, J.M.C., Zomuanpuii, R., Mandal, S.D., & Zothanzama, J. (2021) A new species of Ophiocordyceps (Ophiocordycipitaceae) from Mizoram, India. Phytotaxa, 500(1), 11–20. https://doi.org/10.11646/phytotaxa.500.1.2
Chawngthu, Z., Thachunglura, V. L., Zothanzama, J. & Khumlianlal, J. (2024). Risk assessment of heavy metals in wild mushroom from Mizoram, India. Environmental Pollution and Management, 1, 147–151. https://doi.org/10.1016/j.epm.2024.09.001
Galappaththi, M.C.A., Patabendige, N.M., Premarathne, B.M., Hapuarachchi, K.K., Tibpromma, S., Dai, D.Q., Suwannarach, N., Rapior, S., & Karunarathna, S.C. (2022). A Review of Ganoderma Triterpenoids and Their Bioactivities., 13(1), 24. https://doi.org/10.3390/biom13010024
Isildak, Ö., Turkekul, I., Elmastas, M. & Tuzen, M. (2004). Analysis of heavy metals in some wild-grown edible mushrooms from the middle black sea region, Turkey. Food Chemistry, 86(4), 547–552. https://doi.org/10.1016/j.foodchem.2003.09.007
Kalmis, E., Yildiz, H., Ergonul, B., Kalyoncu, F. & Solak, M. H. (2011). Chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom, Tricholoma anatolicum. Turkey Journal of Biology, 35: 627-633. https://doi.org/10.3906/biy-0909-100.
Karunarathna, S. C., Lu, W., Patabedige, N., Zhao, C.-L. & Hapuarachchi, K. K. (2024). Unlocking the therapeutic potential of edible mushrooms: Ganoderma and their secondary metabolites as novel antiviral agents for combating COVID-19. New Zealand Journal of Botany, 1–59. https://doi.org/10.1080/0028825x.2024.2384453
Karunarathna, S. C., Yang, Z. L., Zhao, R. -L., Vellinga, E. C., Bahkali, A. H., Chukeatirote, E. & Hyde, K. D. (2011). Three new species of Lentinus from northern Thailand. Mycological Progress, 10(4), 389–398. https://doi.org/10.1007/s11557-010-0701-6.
Kaur, J., Singh, J., Bhadariya, V., Gogna, S., Jarial, S., Rasane, P. & Sharma, K. (2022). Edible mushrooms: A source of quality protein. Wild Mushrooms, 169–192. https://doi.org/10.1201/9781003152583-8.
Khan, N., Ajmal, M., Nickten, J., Aslam, S. & Ali, M. (2013). Nutritional value of Pleurotus (Flabellatus) djamor (R-22) cultivated on sawdusts of different woods. Pakistan Journal of Botany. 45. 1105-1108. https://eprints.bbk.ac.uk/id/eprint/9517.
Khumlianlal, J., Huidrom, S., Sharma, K. C., Thachunglura, V. L. & Indira, S. (2024). Proximate Analysis and Mineral Content of Wild Edible Mushrooms from Manipur, India. International Journal of Current Microbiology and Applied Sciences, 13(3), 269–275. https://doi.org/10.20546/ijcmas.2024.1303.026
Khumlianlal, J., Sharma, K. C., Singh, L. M., Mukherjee, P. K., & Indira, S. (2022). Nutritional profiling and antioxidant property of three wild edible mushrooms from North East India. Molecules (Basel, Switzerland), 27(17), 5423. https://doi.org/10.3390/molecules27175423.
Klomklung, N., Karunarathna, S. C., Hyde, K. D. & Chukeatirote, E. (2014). Optimal conditions of mycelial growth of three wild edible mushrooms from northern Thailand. Acta Biologica Szegediensis, 58: 39-43.
Kozarski, M., Klaus, A., Jakovljevic, D., Todorovic, N., Vunduk, J., Petrović, P., Niksic, M., Vrvic, M. M. & van Griensven, L. (2015). Antioxidants of Edible Mushrooms. Molecules (Basel, Switzerland), 20(10), 19489–19525. https://doi.org/10.3390/molecules201019489.
Kumar, R., Tapwal, A., Pandey, S., Borah, R. K., Borah, D. & Borgohain, J. (2013). Macro-fungal diversity and nutrient content of some edible mushrooms of Nagaland, India. Nusantara Bioscience, 5(1):1-7. https://doi.org/10.13057/nusbiosci/n050101.
Kurtzman, R. H. (1975). Mushrooms as a source of food proteins. In: Protein Nutritional Quality of Foods and Feeds, part 2. Ed. Friedman M, Marcel D. New York. pp. 305- 318.
Lallawmsanga, Passari, A. K., Mishra, V. K., Leo, V. V., Singh, B. P., Valliammai Meyyappan, G., Gupta, V. K., Uthandi, S. & Upadhyay, R. C. (2016). Antimicrobial Potential, Identification and Phylogenetic Affiliation of Wild Mushrooms from Two Sub-Tropical Semi-Evergreen Indian Forest Ecosystems. Plos One, 11(11), e0166368. https://doi.org/10.1371/journal.pone.0166368.
Lalrinawmi, H., Vabeikhokhei, J.M.C., Zothanzama, J. & Chawngthu, Z. (2017). Edible mushrooms of Mizoram. Science Vision. 17(4).172-181. https://doi.org/10.33493/scivis.17.04.01.
Lalzarzovi, S.T. & Lalnuntluanga (2022). Plant species diversity in a tropical semi-evergreen forest in Mizoram (northeastern India): assessing the effectiveness of community conservation. Journal of Threatened Taxa, 14(5): 21055–21067. https://doi.org/10.11609/jott.7549.14.5.21055-21067
Largent, D.L. & Stuntz, D.E. (1977). How to Identify Mushrooms to Genus I: macroscopic features. Indiana University, Mad River Press, 86 p.
Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A., Lopez, R., Thompson, J. D., Gibson, T. J. & Higgins, D. G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947–2948. https://doi.org/10.1093/bioinformatics/btm404.
Li, H., Tian, Y., Menolli, N., Ye, L., Karunarathna, S. C., Perez‐Moreno, J., Rahman, M. M., Rashid, M. H., Phengsintham, P., Rizal, L., Kasuya, T., Lim, Y. W., Dutta, A. K., Khalid, A. N., Huyen, L. T., Balolong, M. P., Baruah, G., Madawala, S., Thongklang, N. … Mortimer, P. E. (2021). Reviewing the world’s edible mushroom species: A new evidence‐based classification system. Comprehensive Reviews in Food Science and Food Safety, 20(2), 1982–2014. Portico. https://doi.org/10.1111/1541-4337.12708
Liu, S., Liu, H., Li, J. & Wang, Y. (2022). Research Progress on Elements of Wild Edible Mushrooms. Journal of fungi (Basel, Switzerland), 8(9), 964. https://doi.org/10.3390/jof8090964
Lodge, D.J., Ammirati, J., O’Dell, T.E. & Mueller, G.M. (2004). Collecting and Describing Macrofungi. In Biodiversity of Fungi: Inventory and Monitoring Methods, Mueller, G.M., Bills, G.F., Foster, M.S., Eds. New York, Academic Press, 128–158 p.
Luangharn, T., Hyde, K.D. & Chukeatirote, E. (2014). Proximate Analysis and Mineral Content of Laetiporus sulphureus Strain MFLUCC 12-0546 from Northern Thailand. Chiang Mai Journal of Science, 41(4) : 765-770.
Maaloul, A., Portillo-Lemus, L., Vitou, M., Rapior, S., Morel, S., & Fons, F. (2023). Antioxidant Potential of Several Polypores Mushrooms from the South of France. International Journal of Medicinal Mushrooms, 25(11), 1–10. https://doi.org/10.1615/IntJMedMushrooms.2023050126
Manjunathan, J. & Kaviyarasan, V. (2010). Nutrient distribution from a newly cultivated edible mushroom Lentinus tuberregium (Fr.) Tamil Nadu, India variation in mushroom energy value. J. Chem. Pharm. Res., 2(3):575-578.
Mattila, P., Konko, K., Eurola, M., Pihlava, J-M., Astola, J., Vahteristo, L., Hietaniemi, V., Kumpulainen, J., Valtonen, M. & Piironen, V. (2001). Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms. J. agric. Fd Chem., 49, 2343–2348. https://doi.org/10.1021/jf001525d.
Mleczek, M., Siwulski, M., Budka, A., Niedzielski, P., Mleczek, P., Kuczyńska-Kippen, N., Budzyńska, S., Karolewski, Z., Kalač, P. & Jędryczka, M. (2024). Can the concentration of elements in wild-growing mushrooms be deduced from the taxonomic rank? Environmental Research, 252, 119079. https://doi.org/10.1016/j.envres.2024.119079
Mortimer, P. E., Xu, J., Karunarathna, S. C. & Hyde, K. D. (eds) (2014) Mushrooms for trees and people: a field guide to useful mushrooms of the Mekong region. The World Agroforestry Centre, East Asia, Kunming, China. 125 pp.
Mwangi, R. W., Macharia, J. M., Wagara, I. N. & Bence, R. L. (2022). The antioxidant potential of different edible and medicinal mushrooms. Biomedicine & Pharmacotherapy, 147, 112621. https://doi.org/10.1016/j.biopha.2022.112621.
Nakalembe, I., Kabasa, J. D. & Olila, D. (2015). Comparative nutrient composition of selected wild edible mushrooms from two agro‑ecological zones, Uganda. SpringerPlus, 4:433. https://doi.org/10.1186%2Fs40064-015-1188-z.
Nwanze, P. I., Jatto, W., Oranusi, S. & Josiah, S. J. (2006). Proximate analysis of Lentinus squarrosulus (Mont.) Singer and Psathyrella atroumbonata Pegler. African Journal of Biotechnology, 5 (4), pp. 366-368.
Okwulehie, I.C., Urama, J. & Okorie, D. O. (2014). Chemical Composition and Nutritional Value of Mature and Young Fruiting-Bodies of Pleurotus pulmonarius Produced On Andropogon Gayanus Straw and Khaya Ivorensis Sawdust. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 9, 4(3), 72-77. https://doi.org/10.9790/3008-09347277.
Ouzouni, P.K., Petridis, D., Koller, W.D. & Riganakos, K.A. (2009). Nutritional value and metal content of wild edible mushrooms collected from West Macedonia and Epirus, Greece. Food Chemistry, 115(4), 1575–1580. https://doi.org/10.1016/j.foodchem.2009.02.014.
Paloi, S., Kumla, J., Paloi, B. P., Srinuanpan, S., Hoijang, S., Karunarathna, S. C., Acharya, K., Suwannarach, N., & Lumyong, S. (2023). Termite Mushrooms (Termitomyces), a Potential Source of Nutrients and Bioactive Compounds Exhibiting Human Health Benefits: A Review. Journal of Fungi, 9(1), 112. https://doi.org/10.3390/jof9010112
Pandey, V.V., Sharma, A., Kumar, M., Saxena, J., Kainthola, C., Pandey, A. (2018) Mushroom cultivation: Substantial key to food security. Journal of Applied and Natural Science, 10 (4): 1325-1331.
Pushpa, H. & Purushothama, K. B. (2010). Nutritional analysis of wild and cultivated edible medicinal mushrooms. World Journal Dairy Food Science, 5(2): 140-144.
Raghuramulu, N., Madhavan, N. K. & Kalyanasundaram, S. (2003). A Manual of Laboratory techniquesNational Institute of Nutrition. Indian Council of Medical Research, Hyderabad, India, pp. 56-58.
Rai, P.K. & Lalramnghinglova, H. (2011) Threatened and less known ethnomedicinal plants of an Indo-Burma hotspot region: conservation implications. Environmental Monitoring and Assessment, 178 (1): 53–62. https://doi.org/10.1007/s10661-010-1670-6.
Rai, R.D. & Sohi, H.S. (1988). How protein rich are mushrooms. Indian Horticulture, 33: 2–3.
Raman, J., Lakshmanan, H., Jan, K-Y., Oh, M., Oh, Y-L. & Im, J-H. (2020). Nutritional composition and antioxidant activity of pink oyster mushrooms (Pleurotus djamor var. roseus) grown on a paddy straw substrate. Journal of Mushrooms, 18(3):189-200. https://doi.org/10.14480/JM.2020.18.3.189.
Rampinelli, J.R., Silveira, M.L.L., Gern, R.M.M., Furlan, S.A., Ninow, J.L. & Wisbeck, E. (2010). Nutritional value of Pleurotus djamor cultivated in banana straw. Alim.Nutr.Araraquara, 21(2), 197-202. http://serv-bib.fcfar.unesp.br/.../928.
Rugolo, M., Mascoloti Spréa, R., Dias, M.I., Pires, T.C.S.P., Añibarro-Ortega, M., Barroetaveña, C., Caleja, C., & Barros, L. (2022). Nutritional Composition and Bioactive Properties of Wild Edible Mushrooms from Native Nothofagus Patagonian Forests. Foods, 11(21), 3516. https://doi.org/10.3390/foods11213516
Saha, D., Sundriyal, M., Sundriyal, R.C. (2014). Diversity of food composition and nutritive analysis of edible wild plants in a multi-ethnic tribal land, Northeast India; an important facet for food supply. Indian Journal of Traditional Knowledge, 13(4):698–705.
Sargunam, S.D., Johnsy, G., Samuel, A.S. & Kaviyarasan (2012) Mushrooms in the food culture of the Kaani tribe of Kanyakumari district. Indian Journal of Traditional Knowledge. 11(1), 150-153. http://nopr.niscair.res.in/.../13419.
Shams, R. Singh, J., Dash, K. K. & Dar, A.M. (2022) Comparative study of freeze drying and cabinet drying of button mushroom, Applied Food Research, 2(1). https://doi.org/10.1016/j.afres.2022.100084.
Shin, C. K., Yee, C. F., Shya, L. J. & Atong, M. (2007). Nutritional Properties of Some Edible Wild Mushrooms in Sabah. Journal of Applied Sciences, 7(15), 2216-2221. https://doi.org/10.3923/jas.2007.2216.2221.
Sifat, N., Lovely, F., Zihad, S.M.N.K., Hossain, M.G., Shilpi, J.A., Grice, I.D., Mubarak, M.S., & Uddin, S.J. (2020). Investigation of the nutritional value and antioxidant activities of common Bangladeshi edible mushrooms. Clinical Phytoscience, 6: 88. https://doi.org/10.1186/ s40816-020-00235-3.
Silva, S. O., da Costa S. M. G. & Clemente, E. (2002). Chemical composition of Pleurotus pulmonarius (Fr.) Quel., substrates and residue after cultivation. Brazilian Archives of Biology and Technology, 45(4):531–535. https://doi.org/10.1590/S1516-89132002000600018.
Sousa, A.S., Araújo-Rodrigues, H., & Pintado, M.E. (2023). The Health-promoting Potential of Edible Mushroom Proteins. Current pharmaceutical design, 29(11), 804–823. https://doi.org/10.2174/1381612829666221223103756
Sudheep, N. M. & Sridhar, K. R. (2014). Nutritional composition of two wild mushrooms consumed by the tribals of the Western Ghats of India. Mycology, 5(2), 64–72. https://doi.org/10.1080/21501203.2014.917733.
Teke, A. N., Bi, M. E., Ndam, L. M. & Kinge, T. R. (2021) Nutrient and Mineral Contents of Wild Edible Mushrooms from the Kilum-Ijim Forest, Cameroon. African Journal of Food Science, 15(4), pp. 152-161. https://doi.org/10.5897/AJFS2021.2089.
Thachunglura, V. L., Rai, P. K., Chawgthu, Z., Lalbiakmawia, B., Lalmuansangi, & Zothanzama, J. (2023). Pleurotus giganteus as a Valuable Source of Nutrients. Indian Journal of Science and Technology, 16(sp1), 89–94. https://doi.org/10.17485/ijst/v16sp1.msc12
Thachunglura, V. L., Rai, P. K., Chawngthu, Z., Renthlei, L., Vanlalmalsawmi, R., Bochung, L., Khumlianlal, J. & Zothanzama, J. (2024a). Nutritional composition and mineral contents of common edible wild mushrooms from Mamit and Champhai Districts of Mizoram, India. Food Agricultural Sciences and Technology, 10(1), 42-58.
Thachunglura, V.L., Rai, P.K., Khumlianlal, J., & Zothanzama, J. (2024b) A checklist of wild mushrooms in Mizoram, Northeast India. Plant Pathology & Quarantine, 14(1), 125–142. https://doi.org/10.5943/ppq/14/1/11
Torres-Martínez, B. del M., Vargas-Sánchez, R. D., Torrescano-Urrutia, G. R., Esqueda, M., Rodríguez-Carpena, J. G., Fernández-López, J., Perez-Alvarez, J. A. & Sánchez-Escalante, A. (2022). Pleurotus Genus as a Potential Ingredient for Meat Products. Foods, 11(6), 779. https://doi.org/10.3390/foods11060779.
Valverde, M.E., Hernández-Pérez, T., & Paredes-López, O. (2015). Edible Mushrooms: Improving Human Health and Promoting Quality Life. International Journal of Microbiology, 1–14. https://doi.org/10.1155/2015/376387.
Vishwakarma, P., Singh, P. & Tripathi, N.N. (2017). In-vitro antioxidant activity and nutritional value of four wild oyster mushroom collected from North-Eastern Part of Uttar Pradesh. Mycosphere, 8(4): 592–602. https://doi.org/10.5943/mycosphere/8/4/8.
Wang, D., Sakoda, A. & Suzuki, M. (2001). Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresource Technology, 78(3), 293-300. https://doi.org/10.1016/S0960-8524(01)00002-5.
Wang, M. & Zhao, R. (2023). A review on nutritional advantages of edible mushrooms and its industrialization development situation in protein meat analogues. Journal of Future Foods, 3(1), 1–7. https://doi.org/10.1016/j.jfutfo.2022.09.001
Wang, X.G., Wei, S.Y., Qi, L.L., Yang, Z.F., Tang, J., Liu, Z.L., & Wu, S.J. (2023). Complete mitochondrial genomic sequence of Auricularia delicata (Auriculariaceae), an edible Chinese mushroom. Mitochondrial DNA Part B, 8(10), 1109–1113. https://doi.org/10.1080/23802359.2023.2268759
White, T. J., Bruns, T., Lee, S. & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. PCR Protocols, 315-322.
Zhou, S., Ma, F., Zhang, X. & Zhang, J. (2016). Carbohydrate changes during growth and fruiting in Pleurotus ostreatus. Fungal Biology, 120(6–7), 852–861. https://doi.org/10.1016/j.funbio.2016.03.007.
Zohmangaiha, Vabeikhokhei J. M. C., Zothanzama, J. & Lalrinawmi, H. (2019). Ganoderma Species of Mizoram, India. International Journal of Current Microbiology and Applied Sciences, 8(04): 2871-2879. https://doi.org/10.20546/ijcmas.2019.804.335.
Zohmangaiha, C., Thachunglura, V.L., Lalnuntluanga, & Zothanzama, J. (2023). Morphological characterization and nutritional quality of Lentinula edodes. Journal of Agriculture Food and Environment, 4(3): 9-12. https://doi.org/10.47440/JAFE.2023.4302
Zothanzama, J. (2011). Wood Rotting Fungi of Mizoram .In H. Lalramnghinglova and F. Lalnunmawia (eds). Forest Resources of Mizoram: Conservation and Management. Department of Environmental Science, Mizoram University and Regional Centre, National Afforestation and Eco-development Board; North Eastern Hill University. 345: 326-345.
Zothanzama, J., Blanchette, A. R. & Lalrinawmi H. (2018). Identification of the Edible and Poisonous Mushrooms of Mizoram. Project Report – New Land Use Policy. Memo No. B.15012/1/2016. Govt of Mizoram.

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)