Bioconversion of steroid compounds due to interaction of the bark extract of Michelia champaca with fungi Curvularia verruculosa
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Abstract
Functional groups bioconversion of steroid compounds was observed in the interaction of secondary metabolites of Michelia champaca with Curvularia verruculosa fungal. In an inhibited mechanism, this extract can lyse the cell walls of the fungus. This research aims to determine changes in secondary metabolite compounds involved in the inhibition process of M. champaca bark extract on the growth of the fungus C. verruculosa.The interaction of secondary metabolites of M. champaca with C. verruculosa fungi was observed by biomass test method and bioconversion analysis of steroid compounds was observed by gas chromatography-mass spectroscopy (GC-MS). Based on the biomass test of M. champaca bark extract with 1% concentration can inhibit the biomass formation of C. verruculosa fungal with inhibiting power is 74.73% and at 2% concentration has been able to inhibit completely the biomass formation of C. verruculosa fungal (100%) for a-8 days period of incubation. The bioconversion of the functional groups in steroid compounds was observed by changing allopregnane-7α, 11α-diol-3,20-dione compounds to pregnane-3,20-dione; pregnane-3,11-diol-20-one; and pregnenolone, as well as the bioconversion of β-sitosterol compounds to pseudosarsapogenin-5,20-dien; (3β, 24S)-stigmastane-5-en-3-ol; and (3β, 22E)-stigmastane-5,22-dien- 3-ol.
Article Details
Article Details
Bioconversion, Curvularia verruculosa, extract of Michelia champaca, interaction, steroid compounds
Bawa I G. A. G. (2019). Active Compounds and Antifungal Activity of Bark Extract of Michelia alba to Curvularia verruculosa fungal caused Leaf Spot Disease in Rice (Oryza sativa L.) (Disertacion). Denpasar: Udayana University.
Bawa I G. A. G. (2021). Exploration of Plant Extracts have Antifungal Activity Against Curvularia verruculosa Fungi Causing Leaf Spot Disease in Rice Plants (Oryza sativa L.). Journal of Chemistry, 15(1), 8-15. DOI: https://doi.org/10.24843/JCHEM.2021v15.i01.p02
Bhatti H. N. & Khera R. A. (2012). Biological Transformations of Steroidal Compounds: A Review. Steroids, 77, 1267-1290. DOI: https://doi.org/10.1016/j.steroids.2012.0 7.018
Dembitsky V. M. (2023). Biological Activity and Structural Diversity of Steroids Containing Aromatic Rings, Phosphate Groups, or Halogen Atoms. Molecules. 28, 1-53. https://doi.org/10.3390/molecules28145549
Dias A. C. D. S, Couzinet-Mossion A, Ruiz N, Lakhdar F, Etahiri S, Bertrand S, Ory L, Roussakis C, Pouchus Y. F, Nazih E. H. & Wielgosz-Collin G. (2019). Steroids from Marine-Derived Fungi: Evaluation of Antiproliferative and Antimicrobial Activities of Eburicol. Marine Drugs. 17, 372, 1-15.
Donova M. V. & Egorova O. V. (2012). Microbial Steroid Transformations: Current State and Prospects. Appl Microbiol Biotechnol., 94(6), 1423-1447.DOI: https://doi.org/10.1007/s00253-012-4078-0
Doyle W. I. & Meeks J. P. (2018). Excreted Steroids in Vertebrate Social Communication. The Journal of Neuroscience. 38(14), 3377-3387.
Hashem M, Alamri S. A, Shathan A. A, Alrumman S. A. & Moustafa M. F. M. (2016). Suppression of Phytopathogenic Fungi by Plant Extract of Some Weeds and Possible Mode of Action. British Microbiology Research Journal, 15(3), 1-13.DOI: http://dx.doi.org/10.9734/BMRJ/2016/26629
Holland H. L, Dore S, Xu W. & Brown F. M. (1994). Formation of 5-alpha Steroids by Biotransformation Involving the 5-alpha-Reductase Activity of Penicillium decumbens, Steroids, 59, 642–647. DOI: https://doi.org/10.1016/0039-128x(94)90020-5
Hu Z, He B, Ma L, Sun Y, Niu L. & Zeng B. (2017). Recent Advances in Ergosterol Biosynthesis and Regulation Mechanisms in Saccharomyces cerevisiae. Indian J. Microbiol. 57(3), 270–277. DOI: https://doi.org/10.29208/jsfk.2017.4.1.147
Hunter A. C, Coyle E, Morse F, Dedi C, Dodd H. T. & Koussoroplis S. J. (2009). Transformation of 5-ene Steroids by the Fungus Aspergillus tamarii KITA: Mixed Molecular Fate in Lactonization and Hydroxylation Pathways with Identification of a Putative 3β-Hydroxy-Steroid Dehydrogenase/D5-D4 Isomerase Pathway. Biochim. Biophys. Acta, 1791, 110–117.DOI: https://doi.org/10.1016/j.bbalip.2008.12.005
Kollweov V, Tarlachkov S, Shutov A, Kazantsev A. & Donova M. (2023). Identification of a Gene Encoding a New Fungal Steroid 7-Hydroxylase and Its Functional Characterization in Pichia pastoris Yeast. International Journal of Molecular Science. 24, 1-17. DOI : https://doi.org/10.3390/ijms242417256.
Kristan K. & Rizner T. L. (2012). Steroid-transforming Enzymes in Fungi. Journal of Steroid Biochemistry & Molecular Biology, 129, 79– 91.
Lobastova T. G, Gulevskaia S. A, Sukhodolskaia G. V. & Donova M. V. (2009). Dihydroxylation of Dehydroepiandrosterone in Positions 7alpha and 15alpha by Mycelial Fungi, Prikl. Biokhim. Mikrobiol., 45, 684–689. DOI: http://dx.doi.org/10.1134/S0003683809060076.
Long N. & Zhong G. (2022). The C-22 sterol desaturase Erg5 is responsible for ergosterol biosynthesis and conidiation in Aspergillus fumigatus. J. Microbiol. 60(6),620-626. DOI: https://doi.org/10.1007/s12275-022-1564-7.
Masterson M, Bittar R, Chu H, Yamanaka N. & Haga-Yamanaka S. (2022). Rapid Assessment of Insect Steroid Hormone Entry into Cultured Cells. Frontiers in Physiology. 12, 1-10. DOI : https://doi.org/10.3389/fphys.2021.816058.
Otang W. M, Grierson D. S. & Ndip R. N. (2011). The Effect of the Acetone Extract of Arctotis arctotoides (Asteraceae) on the Growth and Ultrastructure of Some Opportunistic Fungi Associated with HIV/AIDS. Int. J. Mol. Sci., 12, 9226-9235.DOI: https://doi.org/10.3390%2Fijms12129226
Rendowaty A, Djamaan A. & Handayani D. (2017). Optimal Cultivation Time and Antibacterial Activity from Ethyl Acetate Extracts of Symbiont Fungi Aspergillus unguis (WR8) with Haliclona fascigera. Jurnal Sains Farmasi & Klinis, 4(1), 49-54
Sameera V. (2011). Novel Techniques in the Production of Industrially Imperative Products. J Microbial Biochem Technol, R1.003,1-9. DOI: http://dx.doi.org/10.4172/1948-5948.R1-003
Simard J, Ricketts M. L, Gingras S, Soucy P, Feltus F. A. & Melner M. H. (2005). Molecular Biology of the 3β-Hydroxysteroid Dehydrogenase/D5-D4 Isomerase Gene Family, Endocr. Rev., 26, 525–582. DOI: https://doi.org/10.1210/er.2002-0050
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