Pampangouda Pampangouda K. B. Munishamanna H. Gurumurthy


The fermentation of little millet (Panicum sumatrense) carried out by probiotic yeast (Saccharomyces boulardii) and lactic acid bacteria (Lactobacillus acidophilus). The germinated little millets grains were fermented for 5 days grains were dried and grounded in a grinder and sieved. The sieved flours were analyzed for proximate composition, minerals and phytic acid content. The results showed that the little millet fermented by combined inoculation of Saccharomyces boulardii and L. acidophilus significantly enhanced protein content (10.95%) and decreases the fat (2.61%) and carbohydrate (82.01%) and single inoculation with L. acidophilus decreased the fibre (1.05%) and ash (2.30%). Single inoculation of L. acidophilus enhanced the minerals content Ca (41.44mg), Mg (141.08 mg), phosphorus (238.43mg), Fe (7.98 mg) and Zn (4.69mg) and over dual inoculation and control treatments. Similarly, Single inoculation
of Lactobacillus acidophilus showed significant reduction in phytic acid (114.76 mg) content over dual inoculation (126.49 mg) and control (188.95 mg) treatments. The results indicated that fermentation of little millet by L. acidophilus enhanced mineral contents and reduction in phytic acid content.


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Lactobacillus acidophilus, Little millet, Probiotic, Saccharomyces boulardii

Amagloh, F.K., Weber, J.L. Brough, L. Hardacre, A., Mutukumiraa, N. and Coad, J. (2012). Complementary food blends and malnutrition among infants in Ghana: A review and a proposed solution Sci. Res. Essays.,7(9):972-988.
Antony, U. and Chandra, T.S. (1998). Antinutrient reduction and enhancement in protein, starch and mineral availability in fermented flour of finger millet (Eleusine coracana). J. Agric. Food. Chem., 46(7):2578-2582.
AOAC (1980). Official Methods of Analysis, 13th Edition. Association of Official Analytical Chemists, Washington, DC.
Chandru, R., Ranganna, B., Subramanyam, S. and Munishamanna, K.B. (2007) Quality evaluation of ragi flour packaged and marketed as ready to cook product in Karnataka RPF-III submitted to PC cell, PHT, ICAR, CIPHET, Ludhiana.
Chandru, R., Ranganna, B., Subramanyam, S., Palinimuthu, V. and Munishamanna, K. B. (2010). Studies on Extension of shelf life of finger millet flour. Mysore J. Agril. Sci., 44(1): 44-48.
Fasasi, O.S. (2009). Proximate antinutritional factors and functional properties of processed pearl millet (Pennisetumglaucum). J. Food Technol., 7(3): 92-97.
Gabriel, R.A.O. and Akharaiyi, F.C. (2007). Effect of spontaneous fermentation on the chemical composition of thermally treated jack beans (CanavaliaennsiformisL.). Int. J. Biol. Chem. 1(2): 91-97.
Haug, w. and Lantzsch, H.J. (1983).Sensitive method for the rapid determination of phytate in cereals and cereal products.J. Food Sci. Agric., 34: 1423- 1426.
Hoben, H.J. and Somasegaran, P. (1982).Comprison of the pour, spread and drop plate methods for enumeration of Rhizobium spp. In inoculants made from pre-sterilized peat. Appl. Environ. Microbiol., 14: 1246-1247.
Ijarotimi, O.S. (2012). Influence of germination and fermentation on chemical composition, protein quality and physical properties of wheat flour (Triticum aestivum). J. Cereals and Oil seeds India, 3(3): 35-47.
Inyang, C.U. and Zakari, U.M. (2008).Effect of germination and fermentation of pearl millet on proximate chemical and sensory properties of instant “fura”- A Nigerian cereal food. Pak. J. of Nutr., 7(1): 9-12.
Magdi, A.O. (2010).Effect of traditional fermentation process on the nutrient and antinutrient contents of pearl millet during preparation of Lohoh. J. Saudi Soc. Agric. Sci.,10: 1–6.
Mardia, E.E. Abdullahi, H.E. and Nabila, E.Y. (2001). Effect of fermentation and dehulling on starch, total polyphenols, phytic acid content and in vitro protein digestibility of pearl millet. Food chem., 77: 193-196.
Obizoba, I.C. (1988).Nutritive value of malted, dry or wet milled sorghum and corn.Cereal chem., 65: 447-449.
Oyenuga, V.A. (1968). Nigeria foods and feeding stuffs: their chemistry and nutritive value. Ibadan: University Press.
Ranganna, B., Ramya, K.G., Kalpana, B. and Arunkumar, P. (2012). Nutri products from popped kodo and proso millet grains. Mysore J. Agric. Sci., 46(3): 500-504.
Reihaneh, A.G. and Jamuna, P. (2007). The impact of germination and dehulling on nutrients, antinutrients, in vitro iron and calcium bioavailability and in vitro starch and protein digestibility of some legume seeds. LWT., 40: 1292-1299.
Sharma, P. (2008).Minor millets of tribal area, Agrotech Publishing Academy, Udaipur, India.
Sorbhi, R., Ivan, S. and Severino, S.P. (2012). Production of potentially probiotic beverages using single and mixed cereal substrates fermented with lactic acid bacteria culture. Food Microbiol., 30: 239-244.
Sripriya, G., Usha, A. and Chandra, T.S. (1997). Change in carbohydrates, free amino acids, organic acids, phytate and HCL extractability of minerals during germination and fermentation of finger millet (Eleusine coracana). Food Chem., 58(4): 345-350.
Wakil, S.M. and Kazeem, M.O. (2012). Quality assessment of weaning food produced from fermented cereal-legume blends using starters. Int. Food Res. J., 19(4): 1679-1685.
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Pampangouda, P., Munishamanna, K. B., & Gurumurthy, H. (2015). Effect of Saccharomyces boulardii and Lactobacillus acidophilus fermentation on little millet (Panicum sumatrense). Journal of Applied and Natural Science, 7(1), 260-264. https://doi.org/10.31018/jans.v7i1.599
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