R. Kiran P. P. Singh


Bacterial blight of rice caused by Xanthomonas oryzae pv. oryzae is most serious biotic constraint of rice production in Punjab, influenced mainly by environmental factors and nutritional factors applied to the rice crop. Present study aimed at observing the relationship between rain simulation and disease spread. More number of bacterial colonies was recovered from the susceptible rice cultivar (Pusa 1121) plants where no-rain was simulated as compared to those where rain was simulated. Mean bacterial log cfu in rain simulated plant was 4.88 as compared to 5.67 in non rain simulated plots after 1day of inoculation. The population densities of bacteria increased gradually in both the treatments. The log cfu of bacteria was 5.84 in rain simulated plots as compared to 6.28 in non rain simulated plots after 7 days of inoculation. However after 14 days of inoculation, the bacterial population in guttation fluid was found
to be non-significant at 5% level of significance in rain and non rain simulated plots but the bacterial population was found to be less as compared to that after 7 days of inoculation. Rain washed off the guttation fluid which resulted in decrease of bacterial load in the infected leaf. The significant difference at 5 % level of significance was observed between the per cent disease severity and disease spread was also observed in rain simulated plots and no rain simulated plots in cultivar Pusa 1121 which is a bacterial blight resistant cultivar.


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Guttation, log CFU, Pusa 1121, Rain simulation

Butterworth, J. and McCartney, H.A. (1991). The dispersal of bacteria from leaf surfaces by water splash. Journal of Applied Bacteriolology, 71: 484-496.
Bock, C.H., Cook, A.Z., Parker, P.E., Gottwald, T.R. and Graham, J.H. (2012). Short-distance dispersal of splashed bacteria of Xanthomonas citri subsp. citri from canker-infected grapefruit tree canopies in turbulent wind. Plant Pathol, 61: 829–36.
Caicedo, A.L., Williamson, S.H., Hernandez, R.D., Boyko, A., Fledel, A., York, T.L., Polato, N.R., Olsen, KM., Nielson, R., McCouch, S.R., Bastamante, C.D. and Purugganan, M.D. (2007). Genome wide patterns of nucleotide polymorphism in domesticated rice. PLOS Genetics, 3: 1745-1756.
Ding, X.S., Boydston, C.M. and Nelson, R.S. (2001). Presence of Brome mosaic virus in barley guttation fluid and its association with localized cell death response. Phytopathology, 91: 440-448.
Feng, T.Y. and Kuo, T.T. (1975). Chemotactic response of Xanthomonas oryzae toward water droplets collected from water pores on the leaf of rice plants. Botanical Bulletin Academia Sinica, 16: 126-136.
Fitt, B.D.L., McCartney, H.A. and Walklate, P.J. (1989). The role of rain in dispersal of pathogen inoculum. Annual Review of Phytopathology, 27: 241-270.
Gnanamanickam, S.S., Priyadarshini, V.B., Narayanan, N.N., Vasudevan, P. and Kavitha, S. (1999). An overview of bacterial blight disease of rice and strategies for its management. Current Science, 77: 1435- 1443.
Mew, T.W., Mew, I.C. and Haung, J.S. (1984). Scanning of microscopy of virulent and avirulent strains of Xanthomonas campestris pv. campestris on rice leaves. Phytopathology, 74: 635-641.
Mohiuddin, M.S. and Kauffman, H.E. (1975). Multiplication studies of Xanthomonas oryzae isolates on differential rice varieties. Current Science, 44: 637-638.
Mohiuddin, M.S., Verma, J.P. and Rao, Y.P. (1977). Losses due to bacterial blight of rice. Indian Journal of Agricultural Sciences, 47: 221-223.
Roberts, S. J. (1997). Effect of weather conditions on local spread and infection by pea bacterial blight (Pseudomonas syringae pv. pisi). European Journal of Plant Pathology, 103: 711–719.
Singh, S. (2014). Guttation: path, principles and functions. Australian Journal of Botany, 61(7) 497-515. Sharabani, G., Sasson, S.M., Borenstein, M., Shulhani, R., Lofthouse, M., Chalupowicz, L. and Shtienberg, D. (2012). The significance of guttation in the secondary spread of Clavibacter michiganensis subsp. michiganensis in tomato greenhouses. Plant Pathology, 62: 578-586.
Sharma, V.K., Thind, T.S., Singh, P.P., Mohan, C., Arora, J.K. and Raj, P. (2007). Disease-weather relationships and forecasting of bacterial leaf blight of rice. Plant Disease Research, 22: 52-56.
Watanabe, M. and Asaumi, T. (1975). Studies on the physiological changes in the rice plants infected with Xanthomonas oryzae. I. Relationship between the increase in respiratory rate and multiplication of the bacteria in the infected leaves. Annals of Phytopathological Society of Japan, 41: 364-372.
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Kiran, R., & Singh, P. P. (2015). Effect of rain simulation on Xanthomonas oryzae pv. oryzae population density in guttation fluid and on the spread of bacterial blight in rice. Journal of Applied and Natural Science, 7(1), 72-76. https://doi.org/10.31018/jans.v7i1.565
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