Adhesive properties of food and faecal potential probiotic lactobacilli
Article Main
Abstract
In the present investigation, total four isolates of Lactobacillus species i.e. L. casei, L. helveticus, L. brevis and L. fermentum were examined for the cell surface hydrophobicity by bacterial adherence to hydrocarbons assay in LAPTg broth and hydrophobicity was calculated as percentage decrease in Optical Density at 600 nm. The general range of hydrophobicity in Lactobacilli was found in between 6-73%. Remarkably, L. helveticus and L . fermentum showed 73% hydrophobicity in xylene. Higher value of hydrophobicity could point toward a better ability of lactobacilli to adhere to epithelium cells. The outcome of present study concludes that L. helveticus and L. fermentum have good adhesive properties which may help them to adhere to surface epithelium of host cell and further screening with other probiotic attributes could be designated as probiotics.
Article Details
Article Details
Keywords
Lactobacillus, Probiotic, Hydrophobicity, Optical density
References
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Norde, W. and Lyklemm, J. (1993). Protein adsorption and bacterial adhesion to solid surfaces: a colloid- chemical approach. Colloids and Surfaces A, 38:1-13.
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Perers, L. L., Andaker, L., Edebo, O., Stendahl and Tagesson, C. (1977). Association of some enterobacteria with the intestinal mucosa of mouse in relation to their partition in aqueous polymer two-phase systems. Acta Pathologica ET Microbiologica Scandinavica Section B, 85:308-316.
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Rosenberg, M., Bayer, E. A., Delarea, J. and Rosenberg, E. (1982). Role of thin fimbriae in adherence and growth of Acinetobacter calcoaceticus on hexadecane. Applied and Environmental Microbiology, 44:929-937.
Rosenberg, M., Gutnick, D. and Rosenberg, E. (1980). Adherence of bacteria to hydrocarbons: A simple method for measuring cell surface hydrophobicity. FEMS Microbiology Letters, 9:28-33.
Satio, T. (2004). Selection of useful Probiotics lactic acid bacteria from the Lactobacillus acidophilus group and their application to functional foods. Rev. Art. Animal Science Journal, 75:1-13.
Vindderola, C. G. and Reinheimer, J. A. (2003). Lactic acid starter and probiotic bacteria: A comparative in vitro study of probiotic characteristics and biological barrier resistance. Food Research International, 36:895-904.
Yamamoto, K., Miwa, T., Taniguchi, H., Nagano, T., Shimamura, K., Tanaka and Kumagai, T. (1996). Binding specificity of Lactobacillus to glycolipids. Biochemical and Biophysical Research Communications, 228:148-152.
Annuk, H., Hynes, S.O., Hirmo, S., Mikelsaar, M. and Wadstrom, T. (2001). Characterization and differentiation of lactobacilli by lectin typing. Journal of Medical Microbiology, 50:1069-1074.
Busscher, H. I. and Weerkamp, A. H. (1987). Specific and non-specific interactions in bacterial adhesion to solid substrata. FEMS Microbiology Reviews, 46:165-173.
Coconnier, M.H., Klaenhammer, T.R., Kerneis, S., Bernet, M.F. and Servin, A.L. (1992). Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocytes and mucus–secreting cell lines in culture. Applied Environmental Microbiology, 58:2034-2039.
Derjaguin, B. V. and Lanadau, L. (1941). Theory of the stability of strongly charged lyophobic sols and the adhesion of strongly charged particles in solutions of electrolytes. Acta Physiochimica, 14:633-662.
Drisko, J. A., Giles, C. K. and Bischoff, B. J. (2003). Probiotics in health maintenance and disease prevention. Alternative Medicine Review, 8(2): 143-155.
Fuller, R. (1989). Probiotics in man and animals. Journal of Applied Bacteriology, 66:365-378.
Fuller, R. and Gibson, G. R. (1997). Modification of the intestinal micro flora using Probiotics and Prebiotics. Gastroenterology, 32(222):28 31.
Gusils, C., Perez Chaia, A., Gonzalez, S. and Oliver, G. (1999). Lactobacilli isolated from chicken intestines: Potential use as Probiotics. Journal of Food Production, 62 (3):252-256.
Handley, P. S., Harty, D. W. S., Wyatt, J. E., Brown, C. R., Doran, J. P. and Gibbs, A. C. C. (1987). A comparison of the adhesion, co aggregation and cell-surface hydrophobicity properties of fibrillar and fimbriate strains of Enterococcus salivarius. Journal of General Microbiology, 133:3207-3217.
Isberg, R. R. and Barnes, P. (2002). Dancing with the host: flow dependent bacterial adhesion. Cell, 110:1-4.
Jin, L. Z., Ho, Y. W., Abdullah, N., Ali, M. A. and Jalaludin, S. (1996). Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken. Letters in Applied Microbiology, 23:67-71.
Lindahl, M., Faris, A., Wadstrom, T. and Hjerten, S. (1981). A new test based on salting out to measure relative hydrophobicity of bacterial cells. Biochemical and Biophysics Acta, 677:471-476.
Ly, M. H., Aguedo, M., Goudot, S., Le, M. L., Cayot, P., Teixeira, J. A., Le, T. M., Belin, J. M. and Wache, Y. (2008). Interactions between bacterial surfaces and milk proteins, impact on food emulsions stability. Food Hydrocolloid, 22: 742-751.
Matsumura, A., Saito, T., Arakuni, M., Kitazawa, H., Kawai, Y. and Itoh, T. (1999). New binding assay and preparative trial of cell surface lectin from Lactobacillus acidophilus group lactic acid bacteria. Journal of Dairy Science, 82:2525-2529.
Norde, W. and Lyklemm, J. (1993). Protein adsorption and bacterial adhesion to solid surfaces: a colloid- chemical approach. Colloids and Surfaces A, 38:1-13.
Ouwehand, A. C., Salminen, S. and Isolauri, E. (2002). Probiotics: An overview of beneficial effects. Antonie Van Leeuwenhoek, 82:279-289.
Perers, L. L., Andaker, L., Edebo, O., Stendahl and Tagesson, C. (1977). Association of some enterobacteria with the intestinal mucosa of mouse in relation to their partition in aqueous polymer two-phase systems. Acta Pathologica ET Microbiologica Scandinavica Section B, 85:308-316.
Puniya, A.K., Puniya, M., Nagpal, R., Malik, M., Kumar, S., Mishra, V., Dhewa T., Pant, S. and Singh, K. (2008). Functional dairy Foods: A healthy hope. In: A. Kumar, G. Sahal, and R. P. Kaur (Eds.), Lecture Notes: Workshop on Biotechnology Education (pp.195-205). Dehradun: Saraswati Press.
Reid, G., Serivin, A. L., Bruce, A. W. and Busscher, H. J. (1993). Adhesion of three Lactobacillus strains to human urinary and intestinal epithelial cells. Micro-bioscience, 75:57-65.
Rosenberg, M., Bayer, E. A., Delarea, J. and Rosenberg, E. (1982). Role of thin fimbriae in adherence and growth of Acinetobacter calcoaceticus on hexadecane. Applied and Environmental Microbiology, 44:929-937.
Rosenberg, M., Gutnick, D. and Rosenberg, E. (1980). Adherence of bacteria to hydrocarbons: A simple method for measuring cell surface hydrophobicity. FEMS Microbiology Letters, 9:28-33.
Satio, T. (2004). Selection of useful Probiotics lactic acid bacteria from the Lactobacillus acidophilus group and their application to functional foods. Rev. Art. Animal Science Journal, 75:1-13.
Vindderola, C. G. and Reinheimer, J. A. (2003). Lactic acid starter and probiotic bacteria: A comparative in vitro study of probiotic characteristics and biological barrier resistance. Food Research International, 36:895-904.
Yamamoto, K., Miwa, T., Taniguchi, H., Nagano, T., Shimamura, K., Tanaka and Kumagai, T. (1996). Binding specificity of Lactobacillus to glycolipids. Biochemical and Biophysical Research Communications, 228:148-152.
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Adhesive properties of food and faecal potential probiotic lactobacilli. (2009). Journal of Applied and Natural Science, 1(2), 138-140. https://doi.org/10.31018/jans.v1i2.52
