Changes in protein profile and RNA content of Apis mellifera worker pupa on parasitization with Tropilaelaps clareae
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Abstract
Tropilaelaps infestation of Apis mellifera pupa caused reduction in total protein concentration of the infested pupa. This may be due to protein feeding by mite. The additional protein fractions observed by SDS-PAGE and the difference in the nature of free amino acids suggested synthesis of newer proteins perhaps related to the stress response of the host. This opinion is strengthened by the increase in concentration of RNA ( 184.580±36.987 in non-infested pupa and 293.402±50.329 in infested pups) observed in the present study which was responsible for increased transcription of genes encoding antiparasite peptides as reported by other workers.
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Keywords
Apis mellifera, Peptides, Topilaelaps clareae
References
Anderson, D.L. and Morgan M.J. (2007). Genetics and morphological variation of bee parasitic Tropilaelaps mites (Acari:Laelapidae): new and re-redefined species. Exp. Appl. Acrol., 43:1-24.
Badotra, P., Kumar, N.R. and Sharma, S.(2009). Influence of Varroa jacobsoni Oudeman parasatization on the protein profile and RNA content of Apis mellifera L. Worker brood. Haltere, 1(1):25-27.
Badotra, P., Kumar, N.R. and Harjai K. (2013). Varroa causes oxidative stress in Apis mellifera. L. Journal of Global Biosciences, 2(6):199-201.
Ball, B. (1997). Varroa and viruses. In: Varroa! Fight the mite (Eds. Munns, P. and Jones), International bee Research Association, Cardiff. pp.11-15.
Choi, S.Y. and Woo, K.S. (1985). Studies on the bionomics of bee mite Varroa jacobsoni Oudemans and its chemical control. Resp. Off. Rural Dev. Suweon, Korea, 16: 69-76.
De Jong, D., Morse, R.A. and Eickwort, G.C. (1982). Mite pests of honeybees. Ann. Rev. Entomol.,27:229-252.
Delfinado, M. and Baker, E.W. (1961). Tropilaelaps, a new genus of mites from the Philippines (Laelapidae: Acarina). Fieldana Zool., 44:53-56.
Dooremalen, C.V., Stam, E., Gerritsen, L., Cornelissen, B., van der Steen, J., van Langevelde, J., and Blacquiere, T. (2013). Interactive effect of reduced pollen availability and Varroa destructor infestation limits growth and protein content of young honey bees. Journal of Insect Physiology, 59:487–493
Glinski, Z. and Jarosz, J. (1984). Alteration in haemolymph proteins of drone honey bee larvae parasitized by |Varroa jacobsoni. Apidiologie, 15(3):329-338.
Grobov, O.F. (1976). Varroasis in bees. Apimondia, 46:91.
Kolev, D.A. and Shabanov, M.(1989). Changes in |haemolymph protein of drone larvae and pupae parasitized by Varroa jacobsoni. Acta Microbiologica Bulgarica, 24:41-44.
Kumar, R., Kumar, N.R. and Bhalla, O.P. (1993). Studies on the development biology of Tropilaelaps clareae Delfinado and Baker (Acarina: Laelapidae ) vis a vis the threshold stage in life cycle of Apis mellifera Linn. (Hymenoptera: Apidae). Exp. App. Acarol., 17: 621–625.
Kumar, R. And Kumar, N.R. (1995). Mites (Acrina) in close environment of honey bees. Insect Env., 1(2):5-7.
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. |Nature 227:680-685.
Laigo, F.M. and Morse, R.A. (1968). The mite Tropilaelaps clareae in Apis dorsata colonies in the Philippines. Bee World,49:116–118.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Bio. Chem.,193:265-275.
Rinderer, T.E., Oldroyd, B.P., Lekprayoon, C., Wongsiri, S., Boonthal, C. and Thapa, R.(1994). Extended survival of the parasitic honey bee mite Tropilaelaps clareae on adult worker of Apis mellifera and Apis dorsata. J. Apic. Res.,33:171-174.
Schneider, W.C. (1945). Phosphorus compounds in animal tissues. Extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. J. Bio. Chem.,161:293.
Swarup, H., Pathak, S.C. and Arora, S. (1981). Labroatory techniques in modern Biology. Kalyani Publisher, New Delhi.
Weinberg, K.P. and Madel, G. (1985). The influence of the mite Varroa jacobsoni Oud. on the protein concentration and haemolymph volume of the brood of worker bees and drones of the honey bee Apis mellifera L. Apidologie,16(4):421 – 436.
Badotra, P., Kumar, N.R. and Sharma, S.(2009). Influence of Varroa jacobsoni Oudeman parasatization on the protein profile and RNA content of Apis mellifera L. Worker brood. Haltere, 1(1):25-27.
Badotra, P., Kumar, N.R. and Harjai K. (2013). Varroa causes oxidative stress in Apis mellifera. L. Journal of Global Biosciences, 2(6):199-201.
Ball, B. (1997). Varroa and viruses. In: Varroa! Fight the mite (Eds. Munns, P. and Jones), International bee Research Association, Cardiff. pp.11-15.
Choi, S.Y. and Woo, K.S. (1985). Studies on the bionomics of bee mite Varroa jacobsoni Oudemans and its chemical control. Resp. Off. Rural Dev. Suweon, Korea, 16: 69-76.
De Jong, D., Morse, R.A. and Eickwort, G.C. (1982). Mite pests of honeybees. Ann. Rev. Entomol.,27:229-252.
Delfinado, M. and Baker, E.W. (1961). Tropilaelaps, a new genus of mites from the Philippines (Laelapidae: Acarina). Fieldana Zool., 44:53-56.
Dooremalen, C.V., Stam, E., Gerritsen, L., Cornelissen, B., van der Steen, J., van Langevelde, J., and Blacquiere, T. (2013). Interactive effect of reduced pollen availability and Varroa destructor infestation limits growth and protein content of young honey bees. Journal of Insect Physiology, 59:487–493
Glinski, Z. and Jarosz, J. (1984). Alteration in haemolymph proteins of drone honey bee larvae parasitized by |Varroa jacobsoni. Apidiologie, 15(3):329-338.
Grobov, O.F. (1976). Varroasis in bees. Apimondia, 46:91.
Kolev, D.A. and Shabanov, M.(1989). Changes in |haemolymph protein of drone larvae and pupae parasitized by Varroa jacobsoni. Acta Microbiologica Bulgarica, 24:41-44.
Kumar, R., Kumar, N.R. and Bhalla, O.P. (1993). Studies on the development biology of Tropilaelaps clareae Delfinado and Baker (Acarina: Laelapidae ) vis a vis the threshold stage in life cycle of Apis mellifera Linn. (Hymenoptera: Apidae). Exp. App. Acarol., 17: 621–625.
Kumar, R. And Kumar, N.R. (1995). Mites (Acrina) in close environment of honey bees. Insect Env., 1(2):5-7.
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. |Nature 227:680-685.
Laigo, F.M. and Morse, R.A. (1968). The mite Tropilaelaps clareae in Apis dorsata colonies in the Philippines. Bee World,49:116–118.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. J. Bio. Chem.,193:265-275.
Rinderer, T.E., Oldroyd, B.P., Lekprayoon, C., Wongsiri, S., Boonthal, C. and Thapa, R.(1994). Extended survival of the parasitic honey bee mite Tropilaelaps clareae on adult worker of Apis mellifera and Apis dorsata. J. Apic. Res.,33:171-174.
Schneider, W.C. (1945). Phosphorus compounds in animal tissues. Extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. J. Bio. Chem.,161:293.
Swarup, H., Pathak, S.C. and Arora, S. (1981). Labroatory techniques in modern Biology. Kalyani Publisher, New Delhi.
Weinberg, K.P. and Madel, G. (1985). The influence of the mite Varroa jacobsoni Oud. on the protein concentration and haemolymph volume of the brood of worker bees and drones of the honey bee Apis mellifera L. Apidologie,16(4):421 – 436.
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How to Cite
Changes in protein profile and RNA content of Apis mellifera worker pupa on parasitization with Tropilaelaps clareae. (2014). Journal of Applied and Natural Science, 6(2), 693-695. https://doi.org/10.31018/jans.v6i2.519
