In vitro antioxidant and antimicrobial activity of carotenoid pigment extracted from Sporobolomyces sp. isolated from natural source
Article Main
Abstract
The aim of the present study was to isolate and study about the antioxidant and antibacterial activity of carotenoid pigment. Sporobolomyces sp. isolated from the phyllosphere surface of rice plant has found to produce carotenoid pigment. The present investigation was carried out for antioxidant assays viz., DPPH, iron reducing and metal chelating activity. A steady increase in the antioxidant activities was observed in the carotenoid pigment with raising the pigment concentration. In the present study, the maximum antioxidation characteristics of carotenoid by DPPH, iron reducing and metal chelating assays (75.04 %, 1.88 % and 59.32 %) were achieved by pigmentation of Sporobolomyces sp. at the concentration of 100 ?g ml-1. The antibacterial activity was studied on several organisms like Enterococcus sp., Staphylococcus aureus, Streptococcus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. Among the six pathogens, the pigment was found to be more effective against E. coli (2.9 cm) and S. aureus (2.6 cm). This study revealed that yeast carotenoid pigment was a potential source for its use in food and pharmaceutical applications.
Article Details
Article Details
Keywords
Antimicrobial activity, Antioxidant activity, Carotenoid pigment, Sporobolomyces sp.
References
Benzie, I.F. and Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical biochemistry, 239: 70-76
Bhosale, P. (2004). Environmental and cultural stimulants in the production of carotenoids from microorganisms. Applied Microbiology and Biotechnology, 63: 351–361.
Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 29: 1199-1200
Devine, D.A. and Hancock, R.E. (2002). Cationic peptides, distribution and mechanisms of resistance. Current Pharmaceutical, 8: 703-714.
Echavarri, E.C. and Johnson, E.A. (2004). Stimulation of astaxanthin formation in the yeast Xanthophyllomyces dendrorhous by the fungus Epicoccum nigrum. FEMS Yeast Research, 4: 511-519.
Edge, R., Mcgarvey, D.J. and Truscott, T.G. ( 1997). The carotenoids as antioxidants: A review. Journal of Photochemistry and Photobiology, 41: 189-200.
Frengova, G., Simova, E., Pavlova, K., Beshkova, D. and Grigorora, D. (1994). Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate. Biotechnology and Bioengineering, 44: 888-894.
Frengova, G. and Beshkova, M. (2009). Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance. J ournal of Industrial Microbiolology and Biotechnology, 36(2):163-180.
Iqbal, A., Zafar, M. and Faiz, M. (1998). Screening of some medicinal plants for their antimicrobial activities. Journal of Ethnopharmacology, 62: 183-193.
Kaur, R., Arora, S. and Singh, B. (2008). Antioxidant activity of the phenol rich fractions of leaves of Chukrasia tabularis A. Juss. Bioresource Technology, 99: 7682-7698.
Kirakosyan, A., Seymour, E., Kaufman, O.B., Warber, S., Bolling, S. and Chang, S.C. (2003). Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (Hawthorn) subjected to drought and cold stress. Journal of Agricultural and Food Chemistry, 51: 3973–3976.
Kirti, K., Amita, S., Priti, S., Mukesh Kumar, A. and Jyoti, S. (2014). Colorful world of microbes: carotenoids and their applications. Advances in Biology: 1-13.
Kumaresan, N., Sanjay, K.R., Venkatesh, K.S., Kadeppagari, R.K., Vijaylakshmi, G. and Kumar, S.U. (2008). Partially saturated canthaxanthin purified from Aspergillus carbonarius induces apoptosis in prostate cancer cell line. Applied Microbiology and Biotechnology, 80: 467-473.
Latha, B., Jeevaratnam, Murali, H.S. and Manja, K.S. (2005). Influence of growth factors on carotenoid pigmentation of Rhodotorula glutinis DFR-PDY from natural source. Indian Journal of Biotechnology, 4:353-357.
Llic, S.B., Kontantinovic, S.S. and Todorovic, Z.B. (2005). UV-vis analysis and antimicrobial activity of Streptomyces isolates. Medicine and Biology, 121: 44-46.
Lloyd, R.V., Hanna, P.M. and Mason, R.P. (1997). The origin of the hydroxyl radical oxygen in the fenton reaction. Free Radical Biology and Medicine, 22(5): 885–888.
Michalowska, A.G. and Stachowiak, B. (2010). The antioxidant potential of carotenoid extract from Phaffia rhodozyma. Acta Scientiarum Polonorum Technologia Aliment, 9(2):171-188.
Neveen, S.G. (2011). Investigation of the optimum condition and antimicrobial activities of pigments from four potent pigment-producing fungal species. Journal of Life Sciences, 5: 697-711.
Packer, L. and Cadenas, E. (2002). Hand Book of Antioxidants. In: Carotenoids: Antioxidant and other properties of green tea and black tea. Marcel Dekker, Inc., New York. pp. 372.
Padmapriya, C., Murugesan, R. and Gunasekaran, S.(2014). In vitro antioxidant and antibacterial studies of fungal pigment extracts from Parambikulam tiger reserve. Trends in Bioscience, 7(13): 1522-1557.
Puspha, S.M., Vanajakshi, V. and Vijayalakshmi, G. (2009). Antimicrobial activity of ployketides of Monascus purureus. Asian Journal of Microbiolology Biotechnology and Environmental Sciences, 9(2): 225-229.
Sanjay, K.R. (2009). Characterization of Aspergillus carbonarius mutant in relation to xanthin production toxicity studies and fermentation conditions for pigment production. Ph.d Degree, CFTRI, Mysore.
Selvameenal, L., Radhakrishnan, M. and Balagurunathan, R. (2009). Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. International Journal of Pharmaceutical sciences, 4: 499 – 504.
Shimada, K., Fujikawa, K. Yahara, K. and Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and Food Chemistry, 40: 945–948.
Singh, D., Ramesh, K. Vandna, M. and Parikshit, T. (2007). Synthesis and characterization of complexes of cyclohexadeca-6, 8, 14, 16-tetraene and their biological screening. Transition Metal Chemistry, 32: 1051-1055.
Tanaka, M., Kuei, C.W., Nagashima, Y. and Taguchi, T. (1998). Application of antioxidative maillrad reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi, 54(1): 1409–1414.
Tinkler, J. H., F. Biihm, W. Schalch and T.G. Truscott. (1994). Dietary carotenoids protect human cells from damage. Journal of Photochemistry and Photobiology, 26: 283-285.
Ushakumari,U. and Ramanujan, R. (2013). Isolation of astaxanthin from marine yeast and study of its pharmacological activity. International Current Pharmaceutical Journal, 2(3): 67-69.
Bhosale, P. (2004). Environmental and cultural stimulants in the production of carotenoids from microorganisms. Applied Microbiology and Biotechnology, 63: 351–361.
Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 29: 1199-1200
Devine, D.A. and Hancock, R.E. (2002). Cationic peptides, distribution and mechanisms of resistance. Current Pharmaceutical, 8: 703-714.
Echavarri, E.C. and Johnson, E.A. (2004). Stimulation of astaxanthin formation in the yeast Xanthophyllomyces dendrorhous by the fungus Epicoccum nigrum. FEMS Yeast Research, 4: 511-519.
Edge, R., Mcgarvey, D.J. and Truscott, T.G. ( 1997). The carotenoids as antioxidants: A review. Journal of Photochemistry and Photobiology, 41: 189-200.
Frengova, G., Simova, E., Pavlova, K., Beshkova, D. and Grigorora, D. (1994). Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrate. Biotechnology and Bioengineering, 44: 888-894.
Frengova, G. and Beshkova, M. (2009). Carotenoids from Rhodotorula and Phaffia: yeasts of biotechnological importance. J ournal of Industrial Microbiolology and Biotechnology, 36(2):163-180.
Iqbal, A., Zafar, M. and Faiz, M. (1998). Screening of some medicinal plants for their antimicrobial activities. Journal of Ethnopharmacology, 62: 183-193.
Kaur, R., Arora, S. and Singh, B. (2008). Antioxidant activity of the phenol rich fractions of leaves of Chukrasia tabularis A. Juss. Bioresource Technology, 99: 7682-7698.
Kirakosyan, A., Seymour, E., Kaufman, O.B., Warber, S., Bolling, S. and Chang, S.C. (2003). Antioxidant capacity of polyphenolic extracts from leaves of Crataegus laevigata and Crataegus monogyna (Hawthorn) subjected to drought and cold stress. Journal of Agricultural and Food Chemistry, 51: 3973–3976.
Kirti, K., Amita, S., Priti, S., Mukesh Kumar, A. and Jyoti, S. (2014). Colorful world of microbes: carotenoids and their applications. Advances in Biology: 1-13.
Kumaresan, N., Sanjay, K.R., Venkatesh, K.S., Kadeppagari, R.K., Vijaylakshmi, G. and Kumar, S.U. (2008). Partially saturated canthaxanthin purified from Aspergillus carbonarius induces apoptosis in prostate cancer cell line. Applied Microbiology and Biotechnology, 80: 467-473.
Latha, B., Jeevaratnam, Murali, H.S. and Manja, K.S. (2005). Influence of growth factors on carotenoid pigmentation of Rhodotorula glutinis DFR-PDY from natural source. Indian Journal of Biotechnology, 4:353-357.
Llic, S.B., Kontantinovic, S.S. and Todorovic, Z.B. (2005). UV-vis analysis and antimicrobial activity of Streptomyces isolates. Medicine and Biology, 121: 44-46.
Lloyd, R.V., Hanna, P.M. and Mason, R.P. (1997). The origin of the hydroxyl radical oxygen in the fenton reaction. Free Radical Biology and Medicine, 22(5): 885–888.
Michalowska, A.G. and Stachowiak, B. (2010). The antioxidant potential of carotenoid extract from Phaffia rhodozyma. Acta Scientiarum Polonorum Technologia Aliment, 9(2):171-188.
Neveen, S.G. (2011). Investigation of the optimum condition and antimicrobial activities of pigments from four potent pigment-producing fungal species. Journal of Life Sciences, 5: 697-711.
Packer, L. and Cadenas, E. (2002). Hand Book of Antioxidants. In: Carotenoids: Antioxidant and other properties of green tea and black tea. Marcel Dekker, Inc., New York. pp. 372.
Padmapriya, C., Murugesan, R. and Gunasekaran, S.(2014). In vitro antioxidant and antibacterial studies of fungal pigment extracts from Parambikulam tiger reserve. Trends in Bioscience, 7(13): 1522-1557.
Puspha, S.M., Vanajakshi, V. and Vijayalakshmi, G. (2009). Antimicrobial activity of ployketides of Monascus purureus. Asian Journal of Microbiolology Biotechnology and Environmental Sciences, 9(2): 225-229.
Sanjay, K.R. (2009). Characterization of Aspergillus carbonarius mutant in relation to xanthin production toxicity studies and fermentation conditions for pigment production. Ph.d Degree, CFTRI, Mysore.
Selvameenal, L., Radhakrishnan, M. and Balagurunathan, R. (2009). Antibiotic pigment from desert soil actinomycetes; biological activity, purification and chemical screening. International Journal of Pharmaceutical sciences, 4: 499 – 504.
Shimada, K., Fujikawa, K. Yahara, K. and Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and Food Chemistry, 40: 945–948.
Singh, D., Ramesh, K. Vandna, M. and Parikshit, T. (2007). Synthesis and characterization of complexes of cyclohexadeca-6, 8, 14, 16-tetraene and their biological screening. Transition Metal Chemistry, 32: 1051-1055.
Tanaka, M., Kuei, C.W., Nagashima, Y. and Taguchi, T. (1998). Application of antioxidative maillrad reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi, 54(1): 1409–1414.
Tinkler, J. H., F. Biihm, W. Schalch and T.G. Truscott. (1994). Dietary carotenoids protect human cells from damage. Journal of Photochemistry and Photobiology, 26: 283-285.
Ushakumari,U. and Ramanujan, R. (2013). Isolation of astaxanthin from marine yeast and study of its pharmacological activity. International Current Pharmaceutical Journal, 2(3): 67-69.
Issue
Section
Research Articles
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
How to Cite
In vitro antioxidant and antimicrobial activity of carotenoid pigment extracted from Sporobolomyces sp. isolated from natural source. (2014). Journal of Applied and Natural Science, 6(2), 649-653. https://doi.org/10.31018/jans.v6i2.511
