Studies on the effect of ethanolic extract of propolis in BALB/c mice
Article Main
Abstract
Propolis is widely used since ages for the treatment of various ailments. Present study focussed on the toxicity profile of ethanolic extract of propolis on BALB/c mice. The effect of different concentrations of propolis (300, 500, 1000 mg/kg body weight) was analysed by studying the biochemical, haematological and histological changes in mice for 28 days. No significant difference in various parameters were observed in groups of mice treated with propolis and the normal control (p>0.05). Histological findings on liver, spleen, kidney and brain revealed normal architecture. The ethanolic extract of propolis did not produce significant toxic effect in mice and hence can be utilized for nutraceuticals formulations.
Article Details
Article Details
Keywords
Biochemical, Histological, Propolis, Toxicology
References
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ArvouetGrand, A., Lejeune, B., Bastide, P., Pouraat, A. and Legret, P. (1993). Propolis extract. Part 6. Subacute toxicity and cutaneous primary irritation index. Journal de Pharmacie de Belgique, 48: 165-170.
Baker,J.R.(1945). Cytological Technique, 2nd Ed. Metheren, London. pp 310-445.
Bindhu, M. (2007). Guidelines and a survey of current practices evaluation of organ weights for rodent and non rodent toxicity studies. A review of regulatory. Toxicologic Pathology, 35: 741-750.
Cirasino, L., Pisati, A. and Fasani F. (1987). Contact dermatitis from propolis. Contact Dermatitis, 16: 110-111.
Drago, L., Vecchi, D.E., Nicola, L., Gismondo, M.R. (2007). In vitro antimicrobial activity of a novel propolis formulation actichelated propolis). Journal of Applied Microbiology, 103: 1914–1921.
Gessler, M.C., Msuya, D.E., Nkunya, M.H., Mwasunbi, L.B., Schar, A. and Heinrich, M. (1995). Traditional healers in Tanzania: The treatment of malaria with plant remedies. Journal of Ethnopharmacology., 48: 131-144.
Ghisalberti, E.L. (1979). Propolis: a review. Bee world, 60: 59-84.
Hausen, B.M., Wollenweber, E., Senff, H. and Post, B. (1987). Propolis allergy I. Origineti Erties usage and literature review. Contact Dermatitis, 17: 163-170.
Hendi, N.K.K, Naher, H.S. and Al-Charrakh, A.H.(2011). In vitro anti-bacterial and antifungal activity of Iraqi propolis. Journal of Medicinal Plant Research, 5 (20): 5058-5066.
Hollands, I., Vidal, A., Gra, B. and Sotolongo, M. (1991). Evaluation of the sub chronic toxicity of Cuban propolis. Revista Cubana de Cliencias Veterinarias, 22: 91-100.
Jain, N.C. (1986). Hematologic technique. In: Schalm’s Veterinary Hematology 4th Edition, Lea and Febiger, Philadelphia, pp 20–86.
Jasprica, I., Mornar, A., Debeljak, Z., Smolcic Bubalo C., Medic Saric,C, Mayerc, L, Romic, Z., Bucan, K., Balog, T., Sobocanec, S., Sverko, V. (2007).In vivo study of propolis supplementation effects on antioxidative status and red blood cells. Journal of Ethanopharmacology, 110: 548-554.
Kalia, P., Kumar, N.R., Harjai, K. (2013). Phytochemical screening and and antibacterial activity of different extracts of propolis. International Journal of Pharmaceutical and Biological Research, 3(6): 219-222.
Kaur, R. And Kumar, N.R. (2013 a). Pollen foraging activity of Apis mellifera during autumn season in Chandigarh. Halteres, 4:12-14.
Kaur, R., Kalia, P., Kumar, N.R. and Harjai, K. (2013 b). Preliminary studies of different extracts of some honey bee products. Journal of Applied and Natural sciences. 5(2):420-422
Mani, F., Damosceno, H.C.R., Novelli, E.L.B., Martins, E.A.M., Sforcin, J.M. (2006). Propolis: Effect of different concentration, extracts and intake period on seric biochemical variables. Journal of Ethanopharmacology. 105: 95-98.
Mirzoeva, O.K., Grishanin, R.N. and Colder, P.C. (1997). Antimicrobial action of propolis and some of its components: the effect on growth, membrane potential and motility of bacteria. Microbiological Research, 152: 239–246.
Monti, M., Berti, E., Carminati, G. and Cusini, M. (1983). Occupaional and cosmetic dermatitis from propolis. Contact dermatitis, 9:163.
OECD. (2002)Guidelines for the Testing of Chemicals/ Section 4: Health Effects Test No. 423: Acute Oral toxicity – Acute Toxic Class Method. Organization for Economic Cooperation and Development, Paris, France.
OECD. (2008) Repeated dose oral toxicity test method. In: OECD Guidelines for teting of chemicals, Nº 407. Organization for Economic Cooperation and Development, Paris, France
Pan, Y.T., Ghidoni, J. and Elbein, A.D. (1993). The effects of castanospermine and swainsonine on the activity and synthesis of intestinal sucrose. Archives of Biochemistry and Biophysics, 303: 134-144.
Singla, S., Kumar, N.R. and Kaur, J. (2014). In vivo Studies on the protective effect of propolis on doxorubicin induced toxicity in liver of male rats. Toxicology International, 21(2): 191-195.
Thanabhorn, S., Jenjoy, K., Thamaree, S., Ingkaninan, K. and Panthong, A. (2006). Acute and subacute toxicity study of the ethanol extract from Lonicera japonica. Thunb. Journal of Ethanopharmacology, 107: 370-373.
WHO.(2000) General guidelines for methodologies on research and evaluation of traditional medicine. WHO Geneva.
Youdim, K.A., Shukitt-Hale, B., MacKinnon, S., Kalt, W. and Joseph, J.A. (2000). Polyphenolics enhance red blood cell resistance to oxidative stress: in vitro and in vivo. Biochimica et Biophysica Acta, 1523: 117–122.
ArvouetGrand, A., Lejeune, B., Bastide, P., Pouraat, A. and Legret, P. (1993). Propolis extract. Part 6. Subacute toxicity and cutaneous primary irritation index. Journal de Pharmacie de Belgique, 48: 165-170.
Baker,J.R.(1945). Cytological Technique, 2nd Ed. Metheren, London. pp 310-445.
Bindhu, M. (2007). Guidelines and a survey of current practices evaluation of organ weights for rodent and non rodent toxicity studies. A review of regulatory. Toxicologic Pathology, 35: 741-750.
Cirasino, L., Pisati, A. and Fasani F. (1987). Contact dermatitis from propolis. Contact Dermatitis, 16: 110-111.
Drago, L., Vecchi, D.E., Nicola, L., Gismondo, M.R. (2007). In vitro antimicrobial activity of a novel propolis formulation actichelated propolis). Journal of Applied Microbiology, 103: 1914–1921.
Gessler, M.C., Msuya, D.E., Nkunya, M.H., Mwasunbi, L.B., Schar, A. and Heinrich, M. (1995). Traditional healers in Tanzania: The treatment of malaria with plant remedies. Journal of Ethnopharmacology., 48: 131-144.
Ghisalberti, E.L. (1979). Propolis: a review. Bee world, 60: 59-84.
Hausen, B.M., Wollenweber, E., Senff, H. and Post, B. (1987). Propolis allergy I. Origineti Erties usage and literature review. Contact Dermatitis, 17: 163-170.
Hendi, N.K.K, Naher, H.S. and Al-Charrakh, A.H.(2011). In vitro anti-bacterial and antifungal activity of Iraqi propolis. Journal of Medicinal Plant Research, 5 (20): 5058-5066.
Hollands, I., Vidal, A., Gra, B. and Sotolongo, M. (1991). Evaluation of the sub chronic toxicity of Cuban propolis. Revista Cubana de Cliencias Veterinarias, 22: 91-100.
Jain, N.C. (1986). Hematologic technique. In: Schalm’s Veterinary Hematology 4th Edition, Lea and Febiger, Philadelphia, pp 20–86.
Jasprica, I., Mornar, A., Debeljak, Z., Smolcic Bubalo C., Medic Saric,C, Mayerc, L, Romic, Z., Bucan, K., Balog, T., Sobocanec, S., Sverko, V. (2007).In vivo study of propolis supplementation effects on antioxidative status and red blood cells. Journal of Ethanopharmacology, 110: 548-554.
Kalia, P., Kumar, N.R., Harjai, K. (2013). Phytochemical screening and and antibacterial activity of different extracts of propolis. International Journal of Pharmaceutical and Biological Research, 3(6): 219-222.
Kaur, R. And Kumar, N.R. (2013 a). Pollen foraging activity of Apis mellifera during autumn season in Chandigarh. Halteres, 4:12-14.
Kaur, R., Kalia, P., Kumar, N.R. and Harjai, K. (2013 b). Preliminary studies of different extracts of some honey bee products. Journal of Applied and Natural sciences. 5(2):420-422
Mani, F., Damosceno, H.C.R., Novelli, E.L.B., Martins, E.A.M., Sforcin, J.M. (2006). Propolis: Effect of different concentration, extracts and intake period on seric biochemical variables. Journal of Ethanopharmacology. 105: 95-98.
Mirzoeva, O.K., Grishanin, R.N. and Colder, P.C. (1997). Antimicrobial action of propolis and some of its components: the effect on growth, membrane potential and motility of bacteria. Microbiological Research, 152: 239–246.
Monti, M., Berti, E., Carminati, G. and Cusini, M. (1983). Occupaional and cosmetic dermatitis from propolis. Contact dermatitis, 9:163.
OECD. (2002)Guidelines for the Testing of Chemicals/ Section 4: Health Effects Test No. 423: Acute Oral toxicity – Acute Toxic Class Method. Organization for Economic Cooperation and Development, Paris, France.
OECD. (2008) Repeated dose oral toxicity test method. In: OECD Guidelines for teting of chemicals, Nº 407. Organization for Economic Cooperation and Development, Paris, France
Pan, Y.T., Ghidoni, J. and Elbein, A.D. (1993). The effects of castanospermine and swainsonine on the activity and synthesis of intestinal sucrose. Archives of Biochemistry and Biophysics, 303: 134-144.
Singla, S., Kumar, N.R. and Kaur, J. (2014). In vivo Studies on the protective effect of propolis on doxorubicin induced toxicity in liver of male rats. Toxicology International, 21(2): 191-195.
Thanabhorn, S., Jenjoy, K., Thamaree, S., Ingkaninan, K. and Panthong, A. (2006). Acute and subacute toxicity study of the ethanol extract from Lonicera japonica. Thunb. Journal of Ethanopharmacology, 107: 370-373.
WHO.(2000) General guidelines for methodologies on research and evaluation of traditional medicine. WHO Geneva.
Youdim, K.A., Shukitt-Hale, B., MacKinnon, S., Kalt, W. and Joseph, J.A. (2000). Polyphenolics enhance red blood cell resistance to oxidative stress: in vitro and in vivo. Biochimica et Biophysica Acta, 1523: 117–122.
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How to Cite
Studies on the effect of ethanolic extract of propolis in BALB/c mice. (2014). Journal of Applied and Natural Science, 6(2), 638-643. https://doi.org/10.31018/jans.v6i2.509
