Oral microbiota relationship with and without gingivitis in Iraqi patients
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Abstract
Gingivitis is a common oral disease characterized by inflammation of the gum tissues, predominantly caused by microbial dysbiosis. The study aimed to anticipate that the comparative analysis of oral microbiota in Iraqi patients with and without gingivitis would reveal distinct microbial profiles associated with the disease. A cross-sectional study was conducted on Iraqi patients aged 18-65, including males and females. One hundred patients and 50 healthy samples were collected from January to May 2023, diagnosed by Vitek 2 compact system and 16SrRNA. Two hundred and thirty five isolates were obtained form both techniques as four groups:Anaerobic gram-positive (Actinomyces odontolyticus, Actinomyces naeslundii, Streptococcus mutans, Streptococcus anginusom, Streptococcus mitis, Streptococcus oralis, Clostridium sordelli, Group C streptococcus,Streptococcus saliveris, Clostridium histolyticum, Lactobacillus spp., Anaerococcus prevoti, Gemella morbillorum, Turicella Otitidis , Enterococcus casseliflavus, Anaerobic gram-negative(Fusobacterium nucleatum, Vellionela spp., Tannerella forsythia, Fusobacterium mortiifirum, Porphyromonas gingivalis, Prevotella intemedia, Fusobacterium varium, Prevotella disiens, Ggregatibacter actinomycetemcomitans, Enterobacter hormaechei), Facultative gram-positive (Bacillus amyloliquefacieneus,Bacillus atrophaeus, Rothia sp., Bacillus pumilus, Bacillus subtilis, Staphylococcus epidermidis, Bacillus megaterium, Erysipelothrix sp., Staphylococcus aureus, Micrococcus luteus), and Facultative gram-negative bacteria (Pantoea calida, Klebsiella pneumoniae, Pseudomonas putida, Acinetobacter pitti). The anaerobic gram-negative bacteria were the most likely to cause gum diseases. On the other hand the confidence intervals for other group could be attributed to oral disease.Furthermore, these findings can contribute to developing diagnostic and treatment strategies for gingivitis in Iraq.
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16S rRNA gene, Gingivitis, Iraqi patients, Oral microbiota
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