Burkholderia sp. from rhizosphere of Rhododendron arboretum: Isolation, identification and plant growth promotory (PGP) activities
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Abstract
Plant growth promoting rhizobacteria (PGPR) is beneficial bacteria that colonize plant roots and enhance plant growth by wide variety of mechanism like phosphate solubilisation, etc. Use of PGPR has steadily increased in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. The present research work was designed to isolate and characterize the PGP activity of Burkholderia sp. For this purpose rhizospheric soil from Rhododendron arboreum of Kumaun Himalaya was collected and efficient bacterial strain was screened on the basis of phosphate solubilization. Further, assessment of various parameters of plant growth promotion activity was done and enhanced production of IAA (16.4 ?gml-1) and (20.8 ?gml-1) was observed in the presence of 250?gml-1 and 500 ?g ml-1 of tryptophan, respectively. Correspondingly, in respect of 7.8 ?g ml-1 IAA without tryptophan, and their confirmation was executed by TLC. A remarkable change in color from green to reddish-brown zone on CAS plates, suggests the positive result for siderophore production, and finally the seed germination and pot trial experiment depicted the growth index of wheat plant. Therefore, the present study suggests that Burkholderia sp. is beneficial for plant growth promotion.
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Keywords
Burkholderia sp., IAA, PGPR, Phosphate solubilization, Siderophore
References
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Ahmad, F., Ahmad, I. and Khan, M. S. (2005). Indole acetic acid production by the indigenous isolates of azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turk. J. Biol., 29: 29-34.
Ahmad, F., Ahmad, I. and Khan, M. S. (2006). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microb. Res., 36: 1-9.
Anwar, S. M., Siddique, M. T., Verma, A., Rao, Y., Nailwal, T., Ansari, M. W. and Pande, V. (2014). Multitrait plant growth promoting (PGP) rhizobacterial isolates from Brassica juncea rhizosphere keratin degradation and growth promotion. Comm. Integ. Biol., 7( I): e27683-1-9
Bakthavatchalu, S., Shivakumar, S. and Sullia, S. B. (2012). Identification of multi-trait PGPR isolates and evaluation of their potential as biocontrol agents. Acta Biologica Indica., 1(1): 61-67
Bharucha, U., Patel, K. and Trivedi, U. B. (2013). Optimization of indole acetic acid production by Pseudomonas putida UB1 and its effect as plant growth-promoting rhizobacteria on mustard (Brassica nigra). Agric. Res., 2: 215–221.
Buysens, S., Heungens, K., Poppe, J. N. and Hofte, M. (1996). Involvement of pyochelin and pyoverdin in suppression of pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2. Appl. Environ. Microbiol., 62(3): 865–871.
Dastager, S. G., Deepa, C. K. and Pandey, A. (2010). Isolation and characterization of novel plant growth promoting Micrococcus sp NII-0909 and its interaction with cowpea. Plant Physiol. Biochem., 48(12): 987-992.
Gamalero, E., Lingua, G., Capri, F. G., Fusconi, A. and Berta, G. (2004). Colonization pattern of primary tomato roots by Pseudomonas fluorescens A6RI characterized by dilution plating, flow cytometry, fluorescence, confocal and scanning electron microscopy. FEMS Microbiol. Ecol., 48: 79–87.
Goel, A. K., Sindhu, S. S. and Dadarwal, K. R. (2002). Stimulation of nodulation and plant growth of chickpea (Cicer arietinum L.) by Pseudomonas spp. antagonisctic to fungal pathogens. Biol. Fertil. Soil, 36: 391-396.
Holt, J. G., Krieg, N. R. and Sneath, P. H. A. (1994). Bergey’s mannual of determinative Bacteriology, 19th edition, Williams & Wilkins, Baltimore.
Kaur, N. and Sharma, P. (2013). Screening and characterization of native Pseudomonas sp. as plant growth promoting rhizobacteria in chickpea (Cicer arietinum L.) rhizosphere. Afr. J. Microbiol. Res., 7(16): 1465-1474.
Khalid, A., Arshad, M. and Kahir, Z. A. (2004). Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. Appl. Soil. Ecol., 96: 473–480.
Kravchenko, L. V., Azarova, T. S., Makarova, N. M. and Tikhonovich, I. A. (2004). The effect of tryptophane of plant root metabolites on the phytostimulating activity of rhizobacteria. Microibiologia, 73(2): 156–158.
Kumar, A., Kumar, A., Devi, S., Patil, S. and Payal, C. (2012). Isolation, screening and characterization of bacteria from Rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study. Rec. Res. Sci. Technol., 4(1): 01-05.
Mia, M. A. B., Shamsuddin, Z. H. and Mahmood, M. (2012). Effects of rhizobia and plant growth promoting bacteria inoculation on germination and seedling vigor of lowland rice. Afric. J. Biotechnol., 11 (16): 3758-3765.
Naz, I., Bano, A., Rehman, B., Pervaiz, S. and Iqbal, M. (2012). Potential of Azotobacter vinelandii Khsr1 as bio-inoculant. Afric. J. Biotechnol., 11(45): 10368-10372.
Noori, M. S. S. and Saud, H. M. (2012). Potential plant growth-promoting activity of Pseudomonas sp isolated from paddy soil in Malaysia as biocontrol agent. Plant. Pathol. Microbiol., 3(2): 120.
Pikovaskaya, R. I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Micobiologia, 17: 362-370.
Prasanna, R., Joshi, M., Rana, A. and Nain, L. (2010). Modulation of IAA production in cyanobacteria by tryptophan and light. Polish J. Microbiol., 59(2): 99-105.
Rani, M. U. and Reddy, G. A. (2012). Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea. Afric. J. Biotechnol., 11(32): 8085-8091.
Reddy, V., Rao, K.N. and Reddy, S.M. (2011). Production of IAA by earthworm-borne fungi. Scholars Research Library. Ann. Biol. Res., 2 (3): 178-181.
Sakthivel, U. and Karthikeyan, B. (2012). Isolation and characterization of plant growth promoting rhizobacteria (pgpr) from the rhizosphere of coleus forskohlii grown soil. Int. J. Rec. Sci. Res. 3(5): 288 – 296.
Sahasrabudhe, M. M. (2011). Screening of rhizobia for indole acetic acid production, Schol. Res. Lib. Ann. Biol. Res., 2: 460-468.
Shobha, G. and Kumudini, B. S. (2012). Antagonistic effect of the newly isolated PGPR Bacillus spp. on Fusarium oxysporum. Int. J. App. Sci. Engin. Res., 1(3): 463-474.
Sivakumar, T., Shankar, T., Vijayabaskar, P. and Ramasubramanian, V. (2012). Plant growth promoting activity of nickel tolerant Bacillus cereus TS1. J. Agric. Technol., 8: 2101-2113.
Strom, B. and Garhardson, B. (1988). Differential reaction of wheat and Pea genotypes to root inoculation with growth affecting rhizosphere bacteria. Plant. Soil, 109: 263-267.
Sujatha, N. and Ammani, K. (2014). Phosphate solubilisation by the Isolates of Fluorescent pseudomonads. Int. J. Adv. Pharm. Boil. Chem., 3(1): 24-28,
Turan, M., Ataoglu, N. and Sahin, F. (2006). Evaluation of the capacity of phosphate solubilizing bacteria and Fungi on the different form of phosphorous in liquid culture. Sustain. Agric., 28: 99-108.
Ahmad, F., Ahmad, I. and Khan, M. S. (2005). Indole acetic acid production by the indigenous isolates of azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turk. J. Biol., 29: 29-34.
Ahmad, F., Ahmad, I. and Khan, M. S. (2006). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microb. Res., 36: 1-9.
Anwar, S. M., Siddique, M. T., Verma, A., Rao, Y., Nailwal, T., Ansari, M. W. and Pande, V. (2014). Multitrait plant growth promoting (PGP) rhizobacterial isolates from Brassica juncea rhizosphere keratin degradation and growth promotion. Comm. Integ. Biol., 7( I): e27683-1-9
Bakthavatchalu, S., Shivakumar, S. and Sullia, S. B. (2012). Identification of multi-trait PGPR isolates and evaluation of their potential as biocontrol agents. Acta Biologica Indica., 1(1): 61-67
Bharucha, U., Patel, K. and Trivedi, U. B. (2013). Optimization of indole acetic acid production by Pseudomonas putida UB1 and its effect as plant growth-promoting rhizobacteria on mustard (Brassica nigra). Agric. Res., 2: 215–221.
Buysens, S., Heungens, K., Poppe, J. N. and Hofte, M. (1996). Involvement of pyochelin and pyoverdin in suppression of pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2. Appl. Environ. Microbiol., 62(3): 865–871.
Dastager, S. G., Deepa, C. K. and Pandey, A. (2010). Isolation and characterization of novel plant growth promoting Micrococcus sp NII-0909 and its interaction with cowpea. Plant Physiol. Biochem., 48(12): 987-992.
Gamalero, E., Lingua, G., Capri, F. G., Fusconi, A. and Berta, G. (2004). Colonization pattern of primary tomato roots by Pseudomonas fluorescens A6RI characterized by dilution plating, flow cytometry, fluorescence, confocal and scanning electron microscopy. FEMS Microbiol. Ecol., 48: 79–87.
Goel, A. K., Sindhu, S. S. and Dadarwal, K. R. (2002). Stimulation of nodulation and plant growth of chickpea (Cicer arietinum L.) by Pseudomonas spp. antagonisctic to fungal pathogens. Biol. Fertil. Soil, 36: 391-396.
Holt, J. G., Krieg, N. R. and Sneath, P. H. A. (1994). Bergey’s mannual of determinative Bacteriology, 19th edition, Williams & Wilkins, Baltimore.
Kaur, N. and Sharma, P. (2013). Screening and characterization of native Pseudomonas sp. as plant growth promoting rhizobacteria in chickpea (Cicer arietinum L.) rhizosphere. Afr. J. Microbiol. Res., 7(16): 1465-1474.
Khalid, A., Arshad, M. and Kahir, Z. A. (2004). Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. Appl. Soil. Ecol., 96: 473–480.
Kravchenko, L. V., Azarova, T. S., Makarova, N. M. and Tikhonovich, I. A. (2004). The effect of tryptophane of plant root metabolites on the phytostimulating activity of rhizobacteria. Microibiologia, 73(2): 156–158.
Kumar, A., Kumar, A., Devi, S., Patil, S. and Payal, C. (2012). Isolation, screening and characterization of bacteria from Rhizospheric soils for different plant growth promotion (PGP) activities: an in vitro study. Rec. Res. Sci. Technol., 4(1): 01-05.
Mia, M. A. B., Shamsuddin, Z. H. and Mahmood, M. (2012). Effects of rhizobia and plant growth promoting bacteria inoculation on germination and seedling vigor of lowland rice. Afric. J. Biotechnol., 11 (16): 3758-3765.
Naz, I., Bano, A., Rehman, B., Pervaiz, S. and Iqbal, M. (2012). Potential of Azotobacter vinelandii Khsr1 as bio-inoculant. Afric. J. Biotechnol., 11(45): 10368-10372.
Noori, M. S. S. and Saud, H. M. (2012). Potential plant growth-promoting activity of Pseudomonas sp isolated from paddy soil in Malaysia as biocontrol agent. Plant. Pathol. Microbiol., 3(2): 120.
Pikovaskaya, R. I. (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Micobiologia, 17: 362-370.
Prasanna, R., Joshi, M., Rana, A. and Nain, L. (2010). Modulation of IAA production in cyanobacteria by tryptophan and light. Polish J. Microbiol., 59(2): 99-105.
Rani, M. U. and Reddy, G. A. (2012). Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea. Afric. J. Biotechnol., 11(32): 8085-8091.
Reddy, V., Rao, K.N. and Reddy, S.M. (2011). Production of IAA by earthworm-borne fungi. Scholars Research Library. Ann. Biol. Res., 2 (3): 178-181.
Sakthivel, U. and Karthikeyan, B. (2012). Isolation and characterization of plant growth promoting rhizobacteria (pgpr) from the rhizosphere of coleus forskohlii grown soil. Int. J. Rec. Sci. Res. 3(5): 288 – 296.
Sahasrabudhe, M. M. (2011). Screening of rhizobia for indole acetic acid production, Schol. Res. Lib. Ann. Biol. Res., 2: 460-468.
Shobha, G. and Kumudini, B. S. (2012). Antagonistic effect of the newly isolated PGPR Bacillus spp. on Fusarium oxysporum. Int. J. App. Sci. Engin. Res., 1(3): 463-474.
Sivakumar, T., Shankar, T., Vijayabaskar, P. and Ramasubramanian, V. (2012). Plant growth promoting activity of nickel tolerant Bacillus cereus TS1. J. Agric. Technol., 8: 2101-2113.
Strom, B. and Garhardson, B. (1988). Differential reaction of wheat and Pea genotypes to root inoculation with growth affecting rhizosphere bacteria. Plant. Soil, 109: 263-267.
Sujatha, N. and Ammani, K. (2014). Phosphate solubilisation by the Isolates of Fluorescent pseudomonads. Int. J. Adv. Pharm. Boil. Chem., 3(1): 24-28,
Turan, M., Ataoglu, N. and Sahin, F. (2006). Evaluation of the capacity of phosphate solubilizing bacteria and Fungi on the different form of phosphorous in liquid culture. Sustain. Agric., 28: 99-108.
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How to Cite
Burkholderia sp. from rhizosphere of Rhododendron arboretum: Isolation, identification and plant growth promotory (PGP) activities. (2014). Journal of Applied and Natural Science, 6(2), 473-479. https://doi.org/10.31018/jans.v6i2.485
