Effect of pigments of Pseudomonas aeruginosa on adhering and cytotoxicity of A549 cell line
Article Main
Abstract
Pseudomonas aeruginosa gram-negative, bacilli and facultative aerobic, P. aeruginosa cause cystic fibrosis patients, wounds, burns, and immunodeficienct patients, that have many virulence factors such as pyocyanin , cytotoxic ,biofilm formation and motility, Eighty-eight isolates belonging to P. aeruginosa were collected including the 66 clinical isolates obtained from different hospitals in Baghdad and were from different sources and 22 environmental isolates from previous studies of soil near oil fields. Microscopical and cultural characteristics were studied and diagnosed using biochemical tests, VITEC device, their ability to adhere to non-living (Polystyrene), living cell line (A549) and cytotoxicity of bacterial filtrate by MTT method. The results displayed that all isolates belonged to P. aeruginosa. The pigment-forming (pe26 – pc36) isolates and (PE33 – PC31) non-pigment-forming isolates were selected. That all selected bacteria were able to adhere to the Polystyrene and an epithelial carcinoma of lung (A549) was of more than 300 colony formation units in dilution (1:10) ,(1:1000), and (1:10000). The toxicity of the P. aeruginosa filtrate (pc36) isolated from clinical sources and producing pigments was 15.7, 34.5, 44 % at a concentration of 40, 60, 80 % respectively, while the isolate (pc31) that was isolated from clinical sources and non-producing pigment was 28.1, 75.2, 80.9 % at the same concentrations. As for the isolate (pe26),isolated from environmental sources and forming the pigment, the inhibition rate was 38.5, 83.1, 48.8 % at concentrations of 40, 60, 80) % respectively, and the isolate (PE33) that was isolated from environmental sources was 42, 73.4, 74.1 % at concentrations of 40, 60, 80 % respectively. The study will be helpful in evaluating the effect of pigment formation in P. aeruginosa on adhesion.
Article Details
Article Details
Keywords
A549 cell line, Adhesion, Cytotoxicity, Pigments, Pseudomonas aeruginosa
References
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Badaoui, M., Sobolewski, C., Luscher, A., Bacchetta, M., Köhler, T., van Delden, C., ... & Chanson, M. (2023). Targeting HuR-Vav3 mRNA interaction prevents Pseudomonas aeruginosa adhesion to the cystic fibrosis airway epithelium. JCI insight. https://doi.org/10.1172/jci.insight.161961.
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Buré, C., Le Sénéchal, C., Macias, L., Tokarski, C., Vilain, S. & Brodbelt, J. S. (2021). Characterization of isomers of lipid A from Pseudomonas aeruginosa PAO1 by liquid chromatography with tandem mass spectrometry with higher-energy collisional dissociation and ultraviolet photodissociation. Analytical chemistry, 93(9), 4255-4262. https://doi.org/10.1021/acs.analchem.0c05069
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Hall, S., McDermott, C., Anoopkumar-Dukie, S., McFarland, A. J., Forbes, A., Perkins, A. V., Davey, A. K., Chess-Williams, R., Kiefel, M. J., Arora, D. & Grant, G. D. (2016). Cellular effects of pyocyanin, a secreted virulence factor of Pseudomonas aeruginosa. Toxins, 8(8), 236. https://doi.org/10.3390/toxins8080236
Hawdon, N. A., Aval, P. S., Barnes, R. J., Gravelle, S. K., Rosengren, J., Khan, S., Ciofu, O., Johansen, H. K., Høiby, N. & Ulanova, M. (2010). Cellular responses of A549 alveolar epithelial cells to serially collected Pseudomonas aeruginosa from cystic fibrosis patients at different stages of pulmonary infection. FEMS Immunology & Medical Microbiology, 59(2), 207-220. https://doi.org/10.1111/j.1574-695X.2010.00693.x
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Hoffman, C. L., Lalsiamthara, J. & Aballay, A. (2020). Host mucin is exploited by Pseudomonas aeruginosa to provide monosaccharides required for a successful infection. MBio, 11(2), e00060-20. https://doi.org/10.1128/mbio.00060-20
Jawad, L. Q. & Rasheed, H. A. R. R. (2022). Isolation and Purification of Anticancer Protein Exotxin A From Pseudomonas Aeruginosa. Iraqi Journal of Agricultural Sciences, 53(1), 48-56. https://doi.org/10.36103/ijas.v53i1.1507
Khan, F., Pham, D. T. N., Oloketuyi, S. F. & Kim, Y. M. (2020). Regulation and controlling the motility properties of Pseudomonas aeruginosa. Applied microbiology and biotechnology, 104, 33-49. https://doi.org/10.1007/s00253-019-10201-w
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Lindhout, T., Lau, P. C., Brewer, D. & Lam, J. S. (2009). Truncation in the core oligosaccharide of lipopolysaccharide affects flagella-mediated motility in Pseudomonas aeruginosa PAO1 via modulation of cell surface attachment. Microbiology, 155(10), 3449-3460. https://doi.org/10.1099/mic.0.030510-0
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Effect of pigments of Pseudomonas aeruginosa on adhering and cytotoxicity of A549 cell line. (2023). Journal of Applied and Natural Science, 15(3), 954-960. https://doi.org/10.31018/jans.v15i3.4675
