Studies on modulation of hemocyte surface antigen through agglutination reaction under arsenic toxicity in edible mudcrab (Scylla serrata)
##plugins.themes.bootstrap3.article.main##
Abstract
Scylla serrata (Crustacea: Decapoda), which is widely spread on the intertidal mudflat of West Bengal, India's Sundarbans Biosphere Reserves, is a potential aqua crop and an economically significant edible species. One of the larger crab groups in the mangrove swamp of the Sundarbans is thought to be this one. The S. serrata's multifaceted immune response is directly tied to its diverse habitat and survival technique. It lives in dangerous surroundings and is constantly in danger of physiological stress brought on by various xenobiotics, such as arsenic. By producing a number of polyclonal antisera in rabbits (New Zealand White, albino), the study attempted to evaluate the surface antigen against crab hemocytes and murine lymphocytes. Control hemocytes and hemocytes treated to 1 ppm expressed very identical reactivity to antihemocyte sera for the agglutination reaction. The control results, however, shifted when exposed to 2 and 3 ppm of sodium arsenite, indicating arsenic-induced hemocyte surface modification. The agglutination reaction from the control sets of hemocytes that reacted with murine anti-lymphocyte sera gradually, shifted as the quantity of sodium arsenite in the medium of the treatment sets increased. The maximum equivalence zone of murine lymphocyte and hemocyte agglutination 98.6% and 99% respectively suggested a potential epitope sharing between two phylogenetically separate species. The situation may lead to a possible alteration of immune status and make opportunity for pathogenic foreign invaders within the mud crab body. Chronic arsenic exposure indicated a steady decline of edible and demandable S. serrata in the natural habitat of Sundarbans.
##plugins.themes.bootstrap3.article.details##
##plugins.themes.bootstrap3.article.details##
Agglutination, Antisera, Hemocyte Antigen, Scylla serrata, Sodium arsenite
Chakraborty, S., Ray, M. & Ray, S. (2021). Bivalve haemocyte adhesion, aggregation and phagocytosis: A tool to reckon arsenic induced threats to freshwater ecosystem. Fish & Shellfish Immunology, 114, 229-237. DOI: 10.10 16/j.fsi.2021.05.008
Das, S. and Roy Chowdhury, A. (2006). Arsenic: a global monster. Sci. Cult., 72(7- 8): 230 – 237.
Gomez - Caminero, A., Howe, P., Hughes, M., Kenyon, E., Lewis, D. R., Moor, M., Nag, J. Aitio, A. & Becking, G. (2001). Summary. In Environmental Health Criteria 224, Arsenic and Arsenic Compounds (Ed: 2nd), pp. 1-8: World Health Organization, Geneva.
Honigberg, B. M., Friedman, H. & Stepkowskit, S. (1971). Effect of different immunization procedures on agglutination and precipitation reactions of Trichomonas gallinae. J. Parasitol. 57(2), 363 – 369.
Klassen, C. D. (1991). Principles of toxicology. Principles of toxicology. In: Gilman, A. G., Tall, T. W., Nies, A. S., Taylor, P. (Eds.), Pharmacological Basis of Therapeutics, 8th ed. McGraw Hill, pp. 49 – 61.
Marques, M. R. F. & Barracco, M. A. (2000). Lectins, as non-self recognition factors, in crustaceans. Aquacult., 191, 23 – 44.
Ray. S. & Chattopadhyay, S. (2002). Recognition of hemocyte surface antigens of freshwater snail from contaminated habitat by antiserum raised against murine lymphocytes. In Ecology of Polluted Water. Vol. I (Ed. Kumar, A.) A.P.H. Pub. Crop. New Delhi, pp. 61 – 65.
Ray. S. & Chattopadhyay, S. (2003). Methyparathion induced modulation of surface antigen of hemocytes of Bellamya bengalensis. In Environmental challenges of the 21st Century. Chapter 44 (Ed. Kumar, A.) A.P.H. Pub. Crop. New Delhi, pp. 593 – 598.
Ray, S. & Saha, S. (2011). Arsenic toxicity of estuarine mudcrab. LAP LAMBERT Academic Publishing GmbH & Co. KG., Germany.
Rahaman, M. S., Rahman, M. M., Mise, N., Sikder, M. T., Ichihara, G. Uddin, M. K., Kurasaki, M., Ichihara, S. (2021). Environmental arsenic exposure and its contribution to human diseases, toxicity mechanism and management. Environmental Pollution. 289, 117940 (online).
Saha, S. & Ray, S. (2014). Sublethal effect of arsenic on oxidative stress and antioxidant status in the edible crab, Scylla serrata in India. Clean-soil, air, water. 42(9), 1216 – 1222. https://doi.org/10.1002/clen.201300294
Saha, S. (2015). Defence mechanisms of Crustacea against pathogenic and nonpathogenic infection. In Biswas, S., Mitra, M. K., Bar, R. (ed.) Environmental impacts on health: towards a better future. Readers Service Publication, Kolkata, India, pp. 440 – 458.
Saha, S. & Dash, G. (2021a). Climatic Stress on Cultured Scylla sp.: Impact on livelihood and Public Health in West Bengal. International Journal of Researches in Biosciences, Agriculture & Technology. S17, 331 – 344.
Saha, S. & Dash, G. (2021b). Zoonotic Vibrio diversity and diseases in mudcrab fishery: impacts on public health in India. Uttar Pradesh Journal of Zoology. 42(19), 48-58. DOI: 10.13140/RG.2.2.26393.90725
Saha, S., Pradhan, D. & Dash, D. (2022). Studies on Bio-resource, Threats and Conservation of Aquacultural Scylla serrata. In Shashi, P and Sashi, A. (Ed.) Management of natural resources and sustainable regional development. Akinik Publications, New Delhi, India, Chapter 10: pp. 111 – 141. Book DOI: https://doi.org/10.22271/ed.book.203
Shibu Vardhannan, Y. & Radhakrishnan,T. (2002). Acute toxicity of copper, arsenic and HCH to paddy field crab, Paratelphusa hydrodromus (Herb.). J. Environ. Biol. 23, 387 – 392.
Yildiz, H. Y. & Atar, H. H. (2002). Hemocyte classification and differential counts in freshwater crab (Potamon fluviatilis). Turk. J. Vet. Anim. 26, 403-406.
Van de Braak, C. B. T., Taverne, N., Botterblom, M., Van der Knaap, W. P. & Rombout, H. W. M. 2000. Characterization of different morphological features of black tiger shrimp (Penaeus monodon) hemocytes using monoclonal antibodies. Fish Shellfish Immunol., 10, 515 – 530.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
