Dhuha Sabah Nadir Alhan Mohammed Alwan


A significant therapeutic plant from the Malvaceae family is Abutilon hirtum (L.). It is frequently used in traditional medicine to treat a variety of illnesses. Rutin is a flavonoid molecule with commercial value that has anticancer, nutritional, and anti-ageing properties. This research project was carried out to evaluate the effect of abiotic elicitor Methyl Jasmonate (MeJA) at concentrations 0.0, 0.01, 0.02, 0.03 mmol.L-1 on accumulation of rutin by callus cultures  of  Abutilon hirtum L. after 30 days of growth on  Murashige and Skoog (MS) medium to which 2000 µg.L-1 2,4-Dichlorophenoxy acetic acid (2,4-D) + 500 µg.L-1 Kinetin (Kin) were added. The results showed that the best callus stems cultivated on MS growth medium fortified with 2000 µg.L-1 2,4-D + 500 µg.L-1 Kin, recorded the highest fresh weight of 2.887 g after four weeks. This induced callus was characterized by its friable texture. The data of rutin detection in cultures of callus obtained from stems explant of A. hirtum L., using High-Performance Liquid Chromatography (HPLC) indicated the presence of rutin in these cultures by comparison with the rutin standard samples containing a precisely known concentration of a substance for use in quantitative analysis.. Treatment with 0.03 mmol.L-1 of Methyl Jasmonate gave the best rutin amount of 5.606 mg.g-1 was much higher than the control treatment of 1.569 mg.g-1. These data clearly indicated that callus cultures are a potential continuous and constant source of rutin, as a secondary metabolite, and as an alternative to field plants.


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Abutilon hirtum L, Abiotic elicitors, Callus cultures, MeJA, Rutin.

Abarikwu, S. O., Olufemi, P. D., Lawrence, C. J., Ochulor, A. C. & Barikuma, A. M. (2017). Rutin, an antioxidant flavonoid induces glutathione and glutathione peroxidase activities to protect against ethanol effect in cadmium-induced oxidative stress in the testis of adult rats. Andrologia, 49(7):1-12. DOI: 10.1111/and.12696
Ahmad, T. A ., Al-Mahdawe, M. M. & Nadir, Dh. S. (2020). Effect of methyl jasmonate on the production of furanocoumarins in cell suspension cultures of Ruta graveolens L. Pant Cell, Tissue and Organ Culture (PCTOC), 143 (3): 565-571. DOI: 10.1007/s11240-020-01941-z
 Ali, M., Abbasi, B.H. & Ali, G.S. (2015). Elicitation of antioxidant secondary metabolites with jasmonates and gibberellic acid in cell suspension cultures of Artemisia absinthium L. Plant Cell Tiss. Org.  120:1099–1106. DOI: 10.1007/s11240-014-0666-2
Ali, S., Yasmeen, S., Afsa, N., Malik, A., Iqbal, L., Lateef, M. & et. al. (2009). Mutiniside, new antioxidant phenolic glucoside from Abutilon muticum. J. of Asian natural products Res. 11(5): 457-64. DOI: 10.1080/10286020902835539
Boligon, A. A., Jesus, H. S., Plana, M., Elrum, T. F., Cruz, R. C., Mossmann, N. & Athayde, M. L. (2015). Development and validation of an HPLC-DAD analysis for flavonoids in the gel of Scutica buxifolia. J. of Chromatogra. Sci. 53, 1557-1561. DOI: 10.1093/chromsci/bmv056
Brenzel, K. N. (1995). Sunset Western Gardens, 6th edition. Sunset publishing corporation. Menlo Park, Califorinia, U. S. A.
Caglayan, C., Kandemir, F. M. Darendelioglu, E., Yildrim, S., Kucukler, S. and Dortbudak, MB. (2019). Rutin ameliorates mercuric chloride-induced hepatotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis. J. Trace. Elem. Med. Biol. 56(1), 60-68. DOI: 10.1016/j.jtemb.2019.07.011
Chen, R., Hassan, H., Rawlinson, C. & Morgan, d. (2021). Pharmacological properties of rutin and its potential uses for Alzheimers disease. J. of Experi. and Trans. Med. 13(2): 1-12.
Chen, W.Y., Huang, Y. C., Yang, M. L., Lee, C. Y., Yeh, C. H. et al. (2014). Protective effect of rutin on LPS- induced acute lung injury via down-regulation of MIP-2 expression and MMP-9 activation through inhibition of Akt phosphorylation. Int. Immunopharma. 22(2): 409-413. DOI: 10.1016/j.intimp.2014.07.026
Divya, P.; Puthusseri, B. & Neelwarne, B. (2014). The effect of plant regulators on the concentration of carotenoids and phenolic compounds in foliage of coriander. LWT. Food Sci. Technol. 56 101-110. DOI: 10.1016/j.lwt.2013.11.012
Exposito, O., Syklowska-Baranek, K., Moyano, E., Onrubia, M., Bonfill, M., Palazon, J. & Cusido, R. M. (2010). Metabolic responses of Taxus media transformed cell cultures to the addition of methyl jasmonate. Biotechnol. Prog.  26,1145–1153.DOI: 10.1002/btpr.424
Gomaa, A. A., Samy, M. N., Desoukey, S. Y. & Kamel, M. S. (2018). Anti-inflammatory, analgesic, antipyretic and antidiabetic activities of Abutilon hirtum (Lam.) Sweet. J. of Clinic. Phytosci. 4,11. DOI: 10.1186/s40816-018-0069-8
Gorbani, I. (2017). Mechanisms of antidiabetic effects of flavonoid rutin. J. of Biomed. and Pharmaco. 96: 305-312. DOI: 10.1016/j.biopha.2017.10.001
Govindarajan, S., Subiramani, S., Kumar, P. & Kumar, T. S. (2015). Enhanced production of psoralen through elicitors treatment in adventitious root culture of Psoralea corylifolia L. Int. J. of Pharma. and Pharmacce. Sci. 7(1), 146-49.
Hamed, M., Mohamed, M., Refai, L., Hammam, O., El-Ahwany, E., Salah, F., & Hassanein, H. (2015). The active constituents of Pelargonium zonale induced cytotoxicity in human hepatoma cell line HepG2. Int J Pharm Appl, 6(1), 10-9.
Hamed, M. M., Refahy, L. A., & Abdel-Aziz, M. S. (2015). Evaluation of antimicrobial activity of some compounds isolated from Rhamnus cathartica L. Orient J Chem, 31(2), 1133-1140.
Hamed, M. M.; Refahy, L. & Abdel-Aziz, M. (2017). Assessing the bioactivity and antioxidative properties of some compounds isolated from Abutilon hirtum (Lam). Asian J. of Pharma. and Clinic. Res. 10(3): 333-340. DOI:10.22159/ajpcr.2017.v10i3.16229
Hassanein, H. I., El-Ahwany, E. G., Salah, F. M., Hammam, O. A., Refai, L., & Hamed, M. (2011). Extracts of five medicinal herbs induced cytotoxicity in both hepatoma and myeloma cell lines. Journal of Cancer Science & Therapy, 3(10), 239-243. DOI: :10.4172/1948-5956.1 000097
Issa, F. H., Meften, M. T., Tlasha, J. J. A., & Hammood, A. H. (2019). In vitro evaluation of ions, proline and enzymes activity in stressed callus of two tomato hybrids Lycopersicon esculentum Mill. International Journal of Agricultural and Statistical Sciences, 15(1), 91-95.
Jan, R., Asaf, S., Numan, M., Lubna & Min-Kim, K. (2021). Plant secondary metabolite biosynthesis transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy, (11), 968. DOI: 10.3390/agronomy11050968 
Ji,  J.; Feng, Q., Sun, H.,  Zhang, X.,  Li, J. K. &  Gao, J.P. (2019). Response of Bioactive Metabolite and Biosynthesis Related Genes to Methyl Jasmonate eicitation in Codonopsis pilosula. Molecules.  24 (3), 533. DOI: 10.3390/molecules24030533 
Kassem, H. A. (2007). Study of polyphenolic compotents and macro- and micromophological characters of Abutilon hirtum (Lam.) Sweet. Bulletin of Faculty of Pharmacy, Cairo University. 45(3), 173-83.
Kaur, S. & Muthuraman, A. (2016). Therapeutic evaluation of rutin in two-kidney one-clip model of renovascular hypertension in rat. J. of Life Sci. 150, 89-94. DOI: 10.1016/j.lfs.2016.02.080
Liao, C. Y., Smet, W., Brunoud, G., Yoshida, S., Vernoux, T. & Weijers, D. (2015) Reporters for sensitive and quantitative measurement of auxinresponse. Nat Methods, 12:207–210, 2 p . DOI: 10.1038/nmeth.3279
Ljung, K. (2013). Auxin metabolism and homeostasis during plant development. Development 140:943–950. DOI: 10.1242/dev.086363
Meinhart, A. D., Damin, F. M., Calderao, L., Filho, J. T. & Godoy, H. T. (2020). Rutin in herbs and infusions: screening of new sources and consumption estimation. J. of Food Sci. Technol. 40(1), 133-120. DOI: 10.1590/fst.01219 
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physical Plant, 15: 473-497.
Murthy, H. N., Lee, E. J. & Paek, K. Y. (2014). Production of secondary metabolites from cell and organ cultures: Strategies and approaches for biomass improvement and metabolite accumulation. J. of Plant Cell Tissue Organ Cult. 118: 1-6. DOI: 10.1007/s11240-014-0467-7
Nadir, D. S. (2021 b). Initiation and Cultivation of Cell Suspension of Ruta graveolens L. and Studying The Effect of some Elicitors on The Accumulation of Furanocomarin in vitro. A.Ph.D Thesis, submitted to College of Education for pure Science, University of Diyala. Iraq. Pp: 225
Nadir, D.S., Ibrahem, M.M. & Ahmad, T. A. (2021 a). Effect of methyl jasmonate on the production of furanocoumarins in callus cultures of Ruta graveolens L. Indian J. of Eco. 48(3): 950-954.
Nithya, K., Rosheni, N., Brindha, S., Nishmitha, S., Elango, N., Gokila, V. and et al. (2016). Anticataract activity of Abutilon hirtum on glucose induced cataract in goat Eye lens. Int. J. of Pharma. Scie. Rev. and Res. 38(2), 145-148.
Ramawat, K. G. & Merillon, J. M. (2019). Biotechnology, secondary metabolites. Second edition, Published by CRC Press. PP, 586.
Růžička, K., Šimášková, M., Duclercq, J., Petrášek, J., Zažímalová, E., Simon, S., & Benková, E. (2009) Cytokinin regulates root meristem activity via modulation of the polar auxin transport. Proc. Natl. Acad. Sci. USA. 106:4284–4289. DOI: 10.1073/pnas.0900060106
Saeed S., Ali H., Khan T., Kayani W., Khan M.A. (2017). Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth.; a high valued endangered medicinal plant. Physiol. Mol. Biol. Pla. 23:229–237. DOI: 10.1007/s12298-016-0406-
Saini, R. K.; Prashanth, K. V. H.; Shetty, N. P. & Giridhar, P. (2014). Elicitors, SA and MJ enhance carotenoids and tocopherol biosynthesis and expression of antioxidant related genes in Moringa oleifera Lam. leaves. Acta. Physiol. Plant. 36:2695–2704. DOI: 10.1007/s11738-014-1640-7
See, K. S., Bhatt, A. & Keng, C. L. (2011). Effect of sucrose and methyl jasmonate on biomass and anthocyanin production in cell suspension culture of Melastoma malabathricum (Melastomaceae). Rev. biol. Trop. 59 (2): 0034-7744. 
Tahir, S. M., Victor, K. & Abdulkadir, S. (2011). The Effect of 2, 4-Dichlorophenoxy Acetic Acid (2, 4-D) Concentration on Callus Induction in Sugarcane (Saccharum officinarum). Nigerian J. of Basic and App. Sci. 19 (2), 213-217.
Udayakumar, R., Choi C. W., Kim K. T., Kasthurirengan S ., Mariashibu T . S ., Rayan J .J .S . & Ganapathi, A. (2013). In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal . J . M . P . R ., 7(1), 43-52.
Zhang, M., Li S., Nie, L., Chen, Q., Xu, X., Yu, L. & Fu, C. (2015). Two jasmonate-responsive factors, TcERF12 and TcERF15, respectively act as repressor and activator of tasy gene of taxol biosynthesis in Taxus chinensis. Plant Mol. Biol.  89, 463–473. DOI: 10.1007/s11103-015-0382-2
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Nadir, D. S., & Alwan , A. M. (2022). Effect of abiotic elicitor methyl jasmonate on production of rutin in callus cultures of Abutilon hirtum L. Journal of Applied and Natural Science, 14(4), 1493–1499. https://doi.org/10.31018/jans.v14i4.4006
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