L.K. Behera M. R. Nayak D. Nayak D.B. Jadeja


The present investigation was conducted for mass multiplication of Jatropha curcas L. through axillary bud culture. For this nodal segment from 3-5 months old nursery grown plants were used as explants for axillary bud culture. The sterilization treatment involving dipping explants in 0.1 per cent HgCl2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants. The treatment MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1) and number of shoots per explant (6.3). Among different treatments for root initiation, half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1) and length of longest root (4.9 cm) when established shoots were treated with it. Such produced plantlets showed nearly cent per cent survival after hardening and acclimatization. It showed that explants surface sterilized with 0.1 per cent HgCl2 solution for 5 minutes inoculated in MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA and half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC were best in shoot establishment and root development respectively for mass multiplication of J. curcas L. through axillary bud culture.


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Axillary bud culture, In vitro, Jatropha curcas, Mass multiplication, Plantlets

Batra, A., Sardhana, J. and Julfikar, D. A. (2000). High efficiency propagation of Jatropha curcas L.: A medicinally potent plant species. Role of Biotechnology in Medicinal and Aromatic plants, Ukaaz Publication, Hyderabad, pp. 274-285.
Kumar, N. and Reddy, M.P. (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Annals of Applied Biology, 156: 367-375.
Momin, G.G. (2013). Experimental investigation on Jatropha oil as a biodiesel fuel with analysis of its emission characteristics. International Journal of Research in Advent Technology, 1(4): 102-110.
Panse, V.G. and Sukhatme, P.V. (1967). Statistical methods for agricultural workers. Indian Council of Agricultural Research, New Delhi.
Patil, V. and Singh, K. (1991). Oil Gloom to Oil Boom Jatropha curcas-A promising agro-forestry crop. Agro-forestry federation, Maharashtra and Ministry of Environment and Forest, New Delhi.
Rajore, S. and Batra, A. (2005). Efficient plant regeneration via shoot tip explant in Jatropha curcas L. Journal of Plant Biochemistry and Biotechnology, 14(1): 73-75.
Sardana, J., Batra, A. and Ali, D. J. (2000). An expeditious method for regeneration of somatic embryos in Jatropha curcas L. Phytomorphology, 50(3/4): 239-242.
Silva, G. N., Faroni, L. R. A. , Sousa, A. H. and Freitas, R. S. (2012). Bioactivity of Jatropha curcas L. to insect pests of stored products. Journal of Stored Products Research, 48: 111-113.
Sujatha, M. and Mukta, N. (1996). Morphogenesis and plant regeneration from tissue cultures of Jatropha curcas. Plant cell, Tissue and Organ Culture, 44(2): 135-141.
Thakur, S. K. and Shukla, S. V. (2006). Mass multiplication of Jatropha curcas L. through micropropagation. Indian Journal of Agroforestry, 8(2):32-37.
Thomas, R., Sah, N.K. and Sharma, P.B. (2008). Therapeutic biology of Jatropha curcas; a mini review. Current Pharmaceutical Biotechnology, 9(4): 315-324.
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Behera, L., Nayak, M. R., Nayak, D., & Jadeja, D. (2014). In vitro mass multiplication of Jatropha (Jatropha curcas L.) through axillary bud culture. Journal of Applied and Natural Science, 6(1), 189–192. https://doi.org/10.31018/jans.v6i1.398
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