Archana Rani M. Kumar Sanjeev Kumar


In vitro rapid micropropagation of Withania somnifera; has been achieved using shoot apex explants, when cultured on MS medium with different auxins: Indole Butyric Acid (IBA) 0.5-4 mg/l, Indole Acetic Acid (IAA) 2 mg/l and Naphthalene Acetic Acid (NAA) 0.5-3 mg/l and cytokinin: Benzyl Amino Purine (BAP) 0.5-4 mg/l. NAA and BAP stimulated direct as well as callus mediated multiple shoot. IBA alone and in combination with IAA stimulated rhizogenesis, while IBA with NAA proliferated callus-mediated rhizogenesis. The rooted plantlets through sequential hardening process could be established in the field.


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Micropropagation, Shoot apex explant, Rhizogneesis, Withania somnifera

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Rani, A., Kumar, M., & Kumar, S. (2014). In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants. Journal of Applied and Natural Science, 6(1), 159–163. https://doi.org/10.31018/jans.v6i1.393
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