In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants
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Abstract
In vitro rapid micropropagation of Withania somnifera; has been achieved using shoot apex explants, when cultured on MS medium with different auxins: Indole Butyric Acid (IBA) 0.5-4 mg/l, Indole Acetic Acid (IAA) 2 mg/l and Naphthalene Acetic Acid (NAA) 0.5-3 mg/l and cytokinin: Benzyl Amino Purine (BAP) 0.5-4 mg/l. NAA and BAP stimulated direct as well as callus mediated multiple shoot. IBA alone and in combination with IAA stimulated rhizogenesis, while IBA with NAA proliferated callus-mediated rhizogenesis. The rooted plantlets through sequential hardening process could be established in the field.
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Keywords
Micropropagation, Shoot apex explant, Rhizogneesis, Withania somnifera
References
Adhikari, S. R. and Pant, B. (2013). Induction and proliferation of in vitro Mass of Callus of Withania somnifera (L.) Dunal. Research in Plant Sciences, 1(3):58-61.
Agarwal, M. and Kamal, R. (2004). In vitro clonal propagation of Momordica charantia L. Indian J. Biotech., 3:426-430.
Agnihotri, S., Singh, R.R. and Chaturvedi, H.C. (2001). In vitro high frequency regeneration of plantlets of Vigna mungo and their in vitro growth. Indian J. Biotech., 39:916-920.
Asthana, R. and Raina, M.K. (1989). Pharmacology of Withania somnifera (L.)Dun. Indian Drugs,26:199-205.
Atta-Alla, H., and Van Staden, J. (1997). Micropropagation and establishment of Yucca aloifolia.Plant Cell Tiss Org Cult., 48:209-212.
Ghosh, K.C. and Banerjee, N. (2003). Influence of plant growth regulators on in vitro micropropagation of Rauvolfia tetraphyllia L. Phytomorpholog.,55 (1):11-19.
Govindaraju, B., Rao, SR., Venugopal, RB., Kiran, SG., Kaviraj, CP. and Rao, S. (2003). High frequency plant regeneration in Ashwagandha [Withania somnifera (L.) Dunal].: an important medicinal plant. Plant Cell Biotechnology and Molecular Biology. 4 (1/2): 49-56.
Kanungo, S. and Sahoo, S.L. (2011). Direct organogenesis of Withania somnifera L. from apical bud. International Res. J. Biotech., 2(3): 58-61.
Kavidra, N.T., Neelesh, C.S., Vaibhav, T. and Brahma, D. (2000). Micropropagation of Centella asiatica (L.) a valuable medicinal herb. Plant Cell Tiss. Org. Cult., 62:175-179.
Kumar, O. A., Yothirmayee, G. and Tata, S.S. (2011). Multiple shoot regeneration from nodal explants of Ashwagandha (Withania somnifera (L.) Dunal. Asian j. Exp. Biol. Sci., 2(4):636-640.
Kumar, A. O., Jyothirmayee, G. and Subba, T. S. (2011). In vitro multiple shoot induction from shoot tip explants of Ashwaganda–an important medicinal plant. International Journal of Plant, Animal and Environmental Sciences, 1(3):64-68.
Manickam, V. S., Mathavan, R.E. and Antonisamy, R. (2000). Regeneration of Indian ginseng plantlets from stem callus. Plant Cell, Tissue and Organ Culture, 62 (3): 181-185.
Mishra, L.C., Singh, B.B., Dagenais, S. (2000). Scientific basis for the therapeutic use of Withania somnifera (Ashwagandha): A review.Altern. Med. Rev., 5: 334-346.
Misra, H.O., Singh, S. and Kumar, S. (1997). Ashwagandha- Withania somnifera cultivation in India. Central Institute of Medicinal and Aromatic Plants, Lucknow. Farm Bull., No. 005
Nagesh, K.S. (2008). High frequency multiple shoot induction of Curculigo orchioides Gaertn: shoot tip v/s rhizome disc. Taiwania, 53:242-247.
Offord, C.A. and Tyler, J.L. (2009). In vitro propagation of Pimelea spicata R. Br (Thymelaeaceae), an endangered species of the Sydney region, Australia. Plant. Cell. Tiss. Org. Cult., 98:19-23.
Ojha, A. and Choudhary, R. (2013). Callus development and indirect shoot regeneration From leaves and stem explants of Stevia Rebaudiana bertoni. Journal of Cell and Tissue Research, 13(2): 3755-3759.
Patel, P. and Krishnamurthy, R. (2013). Feasibility studies on in vitro mass-scale propagation of Indian Ashwagandha (Withania somnifera) cultivars for commercial purpose.Journal of Pharmacognosy and Phytochemistry, 2(2):168-174.
Pawar, P., Teli, N. and Bhalsing, S. (2001). Micropropagation and organogenetic studies in Withania somnifera (L.) Dunal. Journal of Plant Biology. 28:217-221.
Panwar, G.S., Attitalla, I. H., Guru, S.K. (2011). An efficient in vitro clonal propagation and estimation of reserpine content in different plant parts of Rauwolfia serpentina. American-Eurasian Journal of Scientific Research, 6 (4):217-222.
Rani, G., Arora, S. and Nagpal, A. (2003). Direct rhizogenesis from in vitro leaves of Withania somnifera (L.) Dunal. Journal of Herbs, Spices and Medicinal Plants, 10 (3): 47-54.
Supe, U., Dhote, F. and Roymon, M.G. (2006). In vitro Plant Regeneration of Withania somnifera. Plant Tiss. Cult. Biotech, 16:111-115.
Thomas, T.D. and Shankar, S. (2009). Multiple shoots induction and callus regeneration in Sarcostemma brevistigma Wight and Arnott, a rare medicinal plant. Plant Biotechnol. Rep., 3:67-74.
Udayakumar, R., Choi, C.W., Kim, K.T., Kim, S.C., Kasthurirengan, S., Mariashibu, T.S., Sahaya, R.J. J. and Ganapathi, A. (2013). In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal. J Medicinal Plants Res., 7(1): 43-52.
Agarwal, M. and Kamal, R. (2004). In vitro clonal propagation of Momordica charantia L. Indian J. Biotech., 3:426-430.
Agnihotri, S., Singh, R.R. and Chaturvedi, H.C. (2001). In vitro high frequency regeneration of plantlets of Vigna mungo and their in vitro growth. Indian J. Biotech., 39:916-920.
Asthana, R. and Raina, M.K. (1989). Pharmacology of Withania somnifera (L.)Dun. Indian Drugs,26:199-205.
Atta-Alla, H., and Van Staden, J. (1997). Micropropagation and establishment of Yucca aloifolia.Plant Cell Tiss Org Cult., 48:209-212.
Ghosh, K.C. and Banerjee, N. (2003). Influence of plant growth regulators on in vitro micropropagation of Rauvolfia tetraphyllia L. Phytomorpholog.,55 (1):11-19.
Govindaraju, B., Rao, SR., Venugopal, RB., Kiran, SG., Kaviraj, CP. and Rao, S. (2003). High frequency plant regeneration in Ashwagandha [Withania somnifera (L.) Dunal].: an important medicinal plant. Plant Cell Biotechnology and Molecular Biology. 4 (1/2): 49-56.
Kanungo, S. and Sahoo, S.L. (2011). Direct organogenesis of Withania somnifera L. from apical bud. International Res. J. Biotech., 2(3): 58-61.
Kavidra, N.T., Neelesh, C.S., Vaibhav, T. and Brahma, D. (2000). Micropropagation of Centella asiatica (L.) a valuable medicinal herb. Plant Cell Tiss. Org. Cult., 62:175-179.
Kumar, O. A., Yothirmayee, G. and Tata, S.S. (2011). Multiple shoot regeneration from nodal explants of Ashwagandha (Withania somnifera (L.) Dunal. Asian j. Exp. Biol. Sci., 2(4):636-640.
Kumar, A. O., Jyothirmayee, G. and Subba, T. S. (2011). In vitro multiple shoot induction from shoot tip explants of Ashwaganda–an important medicinal plant. International Journal of Plant, Animal and Environmental Sciences, 1(3):64-68.
Manickam, V. S., Mathavan, R.E. and Antonisamy, R. (2000). Regeneration of Indian ginseng plantlets from stem callus. Plant Cell, Tissue and Organ Culture, 62 (3): 181-185.
Mishra, L.C., Singh, B.B., Dagenais, S. (2000). Scientific basis for the therapeutic use of Withania somnifera (Ashwagandha): A review.Altern. Med. Rev., 5: 334-346.
Misra, H.O., Singh, S. and Kumar, S. (1997). Ashwagandha- Withania somnifera cultivation in India. Central Institute of Medicinal and Aromatic Plants, Lucknow. Farm Bull., No. 005
Nagesh, K.S. (2008). High frequency multiple shoot induction of Curculigo orchioides Gaertn: shoot tip v/s rhizome disc. Taiwania, 53:242-247.
Offord, C.A. and Tyler, J.L. (2009). In vitro propagation of Pimelea spicata R. Br (Thymelaeaceae), an endangered species of the Sydney region, Australia. Plant. Cell. Tiss. Org. Cult., 98:19-23.
Ojha, A. and Choudhary, R. (2013). Callus development and indirect shoot regeneration From leaves and stem explants of Stevia Rebaudiana bertoni. Journal of Cell and Tissue Research, 13(2): 3755-3759.
Patel, P. and Krishnamurthy, R. (2013). Feasibility studies on in vitro mass-scale propagation of Indian Ashwagandha (Withania somnifera) cultivars for commercial purpose.Journal of Pharmacognosy and Phytochemistry, 2(2):168-174.
Pawar, P., Teli, N. and Bhalsing, S. (2001). Micropropagation and organogenetic studies in Withania somnifera (L.) Dunal. Journal of Plant Biology. 28:217-221.
Panwar, G.S., Attitalla, I. H., Guru, S.K. (2011). An efficient in vitro clonal propagation and estimation of reserpine content in different plant parts of Rauwolfia serpentina. American-Eurasian Journal of Scientific Research, 6 (4):217-222.
Rani, G., Arora, S. and Nagpal, A. (2003). Direct rhizogenesis from in vitro leaves of Withania somnifera (L.) Dunal. Journal of Herbs, Spices and Medicinal Plants, 10 (3): 47-54.
Supe, U., Dhote, F. and Roymon, M.G. (2006). In vitro Plant Regeneration of Withania somnifera. Plant Tiss. Cult. Biotech, 16:111-115.
Thomas, T.D. and Shankar, S. (2009). Multiple shoots induction and callus regeneration in Sarcostemma brevistigma Wight and Arnott, a rare medicinal plant. Plant Biotechnol. Rep., 3:67-74.
Udayakumar, R., Choi, C.W., Kim, K.T., Kim, S.C., Kasthurirengan, S., Mariashibu, T.S., Sahaya, R.J. J. and Ganapathi, A. (2013). In vitro plant regeneration from epicotyl explant of Withania somnifera (L.) Dunal. J Medicinal Plants Res., 7(1): 43-52.
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How to Cite
In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants. (2014). Journal of Applied and Natural Science, 6(1), 159-163. https://doi.org/10.31018/jans.v6i1.393
