Standardization of leaf age for radical scavenging activity in Terminalia arjuna
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Abstract
Terminalia arjuna is one of the many such plant that used in indigenous system of medicine for curing different diseases. For effective formulation of herbal drug selection of proper age of the plant is imperative. In the present study, the T. arjuna plant (leaves) was collected from the Botanical garden of Saurashtra University. The leaves were grouped in to various size and growth analysis was performed. Each extract homogenized in methanol and was used for chlorophyll estimation and antioxidant activities. Radical scavenging activity of the methanol extract of different stages leaves were determined using a stable free radical 2, 2-diphenyl-1-picryl hydrazyl (DPPH), using Micro plate assay. Further the phenolic compounds were separated by Reverse phase high pressure liquid chromatography analysis (RP-HPLC). At the leaf stage when maximum antioxidant activity was observed, phenol like Gallic acid showed distinct peak. The role of phenolic compounds in antioxidant activities is discussed.
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Keywords
Antioxidant activity, Chlorophyll content, Growth, Terminalia arjuna
References
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Bhalodi, P. (2004). Screening of some medicinal plants of Saurashtra region for antioxidant activity. M.sc. Dissertation, Department of Bioscience, Saurashtra University, Rajkot.
Chaudhari, M. and Mengi, S. (2006). Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats. Phytotherapy Research, 20: 799-805.
Cheng, H.Y., Lin, C.C. and Lin, T.C. (2002). Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn. Antivirus Research,55:447-455.
Chong, N.J. and Aziz, Z. (2011). A systematic review on chemical constituents of Centella asiatica. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2(3): 445-459.
Devi, R., Narayan, S., Vani, G., Srinivasan, P., Mohan, K., Sabitha, K. and Devi, C. (2007). Ulcer protective effect of Terminalia arjuna on gastric mucosal defensive mechanism in experimental rats. Phytotherapy Research, 21:762-767.
Dwivedi, S.V. (2007). Terminalia arjuna Weight and Arn. a useful drug for cardiovascular disorders. Journal of Ethnopharmacology, 114(2):114-129.
Gokani, S.J. and Thaker, V.S.(2001). Accumulation of abscisic acid in cotton fiber and seed of normal and abnormal bolls. Journal of Agriculture Science,137:444-451.
Halder, S., Bharal, S.N., Mediratta, P.K., Kaur, I. and Sharma, K.K. (2009). Anti-inflammatory, immunomodulatory and antinociceptive activity of Terminalia arjuna bark powder in mice and rats. Indian Journal of Experimental Biology, 47:577-583.
Kaur, K., Arora, S., Kumar, S. and Nagpala, A. (2002). Antimutagenic activities of acetone and methanol fractions of Terminalia arjuna. Food Chemical Toxicology, 40:1475-1482.
Kaur, R., Kapoor, K. and Kaur, H. (2011). Plants as a source of anticancer agents. J. Natural Product Plant Resource, 1(1):119-124.
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Lukmanul, F., Girija, A. and Boopathy, R. (2008). Antioxidant property of selected ocimum species and their secondary metabolite content. Journal of Medicinal plants, 2(9):250-257.
Manna, P., Sinha, P.M. and Sil, P.C. (2008). Protection of arsenic induced testicular oxidative stress by arjunolic acid. Redox Report, 13:67-77.
Mohanty, S. and Cock, I.E. (2012). The chemotherapeutic potential of Terminalia ferdinandiana Phytochemistry and Bioactivity. Pharmacognosy reviews, 6(11): 29-36.
Muchadia, and Thaker, V.S. (2006). Leaf area meter software®. M.sc.Dissertation, Department of Bioscience, Saurashtra University, Rajkot.
Patel, D. and Thaker V. (2007). Role of cellwall stiffening enzymes in internodes development of Merremia emarginata. General and Applied Plant Physiology, 33(1-2):25-39.
Rabadia, V.S., Thaker, V.S. and Singh, Y.D. (1999). Relationship between water content and growth of seed and fiber of three cotton genotypes. Journal of Agriculture Crop Science,183:255-261.
Raghavan, B. and Kumari, S.K. (2006). Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats. Indian Journal of Physiology and Pharmacology, 50:133-142.
Ram, A. P., Lauria, R., Gupta, P., Kumar, P. and Sharma V.N.(1997). Hypocholesterolaemic effects of Terminalia arjuna tree bark. Journal of Ethnopharmacolog, 55(3):165-169.
Reddy, T.K., Seshadri, P., Reddy, K., Jagetia, J.C. and Reddy, C.D. (2008). Effect of Terminalia arjuna extract on adriamycin induced DNA damage. Phytotherapy Research, 22:1188-1194.
Solecki, R.S. (1975). Shanidar IV a neanderthal flower burial of Northern Iraq. Science, 190(28):880-881.
Subasini, U., Rajamanickam, G.V., Dubey, G.P., Prabu, P.C. and Sahayam, C.S. (2007). Hydroalcoholic extract of Terminalia arjuna: A potential hepatoprotective herb. Journal of Biological Sciences, 7:255-262.
Tapsell, L.C., Hemphill, I. and Cabiac, L. (2006). Health benefits of herbs and species: the past, the present the future. Medical Journal of Australia, 185:24-54.
Velázquez, E., Tournier, H.A., Mordujovich de Buschiazzo, N., Saavedra, G. and Schinella, G.R. (2003). Antioxidant activity of Paraguayan plant extracts. Fitoterapia, 74:91-97.
Warrier, P.K., Nambiar, V.P. and Kutty C.B. (1996). Indian Medicinal plants. A Compendium of 500 species. Vol.1, Orient longman Pvt. Ltd., Madras, India.
Bhalodi, P. (2004). Screening of some medicinal plants of Saurashtra region for antioxidant activity. M.sc. Dissertation, Department of Bioscience, Saurashtra University, Rajkot.
Chaudhari, M. and Mengi, S. (2006). Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats. Phytotherapy Research, 20: 799-805.
Cheng, H.Y., Lin, C.C. and Lin, T.C. (2002). Antiherpes simplex virus type 2 activity of casuarinin from the bark of Terminalia arjuna Linn. Antivirus Research,55:447-455.
Chong, N.J. and Aziz, Z. (2011). A systematic review on chemical constituents of Centella asiatica. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2(3): 445-459.
Devi, R., Narayan, S., Vani, G., Srinivasan, P., Mohan, K., Sabitha, K. and Devi, C. (2007). Ulcer protective effect of Terminalia arjuna on gastric mucosal defensive mechanism in experimental rats. Phytotherapy Research, 21:762-767.
Dwivedi, S.V. (2007). Terminalia arjuna Weight and Arn. a useful drug for cardiovascular disorders. Journal of Ethnopharmacology, 114(2):114-129.
Gokani, S.J. and Thaker, V.S.(2001). Accumulation of abscisic acid in cotton fiber and seed of normal and abnormal bolls. Journal of Agriculture Science,137:444-451.
Halder, S., Bharal, S.N., Mediratta, P.K., Kaur, I. and Sharma, K.K. (2009). Anti-inflammatory, immunomodulatory and antinociceptive activity of Terminalia arjuna bark powder in mice and rats. Indian Journal of Experimental Biology, 47:577-583.
Kaur, K., Arora, S., Kumar, S. and Nagpala, A. (2002). Antimutagenic activities of acetone and methanol fractions of Terminalia arjuna. Food Chemical Toxicology, 40:1475-1482.
Kaur, R., Kapoor, K. and Kaur, H. (2011). Plants as a source of anticancer agents. J. Natural Product Plant Resource, 1(1):119-124.
Khory, R.N. (1867). The Bombay material medica and their therapeutics. Renina’s Union Press.
Lukmanul, F., Girija, A. and Boopathy, R. (2008). Antioxidant property of selected ocimum species and their secondary metabolite content. Journal of Medicinal plants, 2(9):250-257.
Manna, P., Sinha, P.M. and Sil, P.C. (2008). Protection of arsenic induced testicular oxidative stress by arjunolic acid. Redox Report, 13:67-77.
Mohanty, S. and Cock, I.E. (2012). The chemotherapeutic potential of Terminalia ferdinandiana Phytochemistry and Bioactivity. Pharmacognosy reviews, 6(11): 29-36.
Muchadia, and Thaker, V.S. (2006). Leaf area meter software®. M.sc.Dissertation, Department of Bioscience, Saurashtra University, Rajkot.
Patel, D. and Thaker V. (2007). Role of cellwall stiffening enzymes in internodes development of Merremia emarginata. General and Applied Plant Physiology, 33(1-2):25-39.
Rabadia, V.S., Thaker, V.S. and Singh, Y.D. (1999). Relationship between water content and growth of seed and fiber of three cotton genotypes. Journal of Agriculture Crop Science,183:255-261.
Raghavan, B. and Kumari, S.K. (2006). Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats. Indian Journal of Physiology and Pharmacology, 50:133-142.
Ram, A. P., Lauria, R., Gupta, P., Kumar, P. and Sharma V.N.(1997). Hypocholesterolaemic effects of Terminalia arjuna tree bark. Journal of Ethnopharmacolog, 55(3):165-169.
Reddy, T.K., Seshadri, P., Reddy, K., Jagetia, J.C. and Reddy, C.D. (2008). Effect of Terminalia arjuna extract on adriamycin induced DNA damage. Phytotherapy Research, 22:1188-1194.
Solecki, R.S. (1975). Shanidar IV a neanderthal flower burial of Northern Iraq. Science, 190(28):880-881.
Subasini, U., Rajamanickam, G.V., Dubey, G.P., Prabu, P.C. and Sahayam, C.S. (2007). Hydroalcoholic extract of Terminalia arjuna: A potential hepatoprotective herb. Journal of Biological Sciences, 7:255-262.
Tapsell, L.C., Hemphill, I. and Cabiac, L. (2006). Health benefits of herbs and species: the past, the present the future. Medical Journal of Australia, 185:24-54.
Velázquez, E., Tournier, H.A., Mordujovich de Buschiazzo, N., Saavedra, G. and Schinella, G.R. (2003). Antioxidant activity of Paraguayan plant extracts. Fitoterapia, 74:91-97.
Warrier, P.K., Nambiar, V.P. and Kutty C.B. (1996). Indian Medicinal plants. A Compendium of 500 species. Vol.1, Orient longman Pvt. Ltd., Madras, India.
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How to Cite
Standardization of leaf age for radical scavenging activity in Terminalia arjuna. (2014). Journal of Applied and Natural Science, 6(1), 76-80. https://doi.org/10.31018/jans.v6i1.378
