Plant growth-promoting rhizobacteria mediated moisture stress alleviation in the early stages of Rice (Oryza sativa L.) variety CO 51
Article Main
Abstract
Drought is one of the abiotic stresses that have a significant impact on agricultural growth across the world. Plant growth-promoting rhizobacteria (PGPR) inoculation in rice plants may be a viable and environmentally acceptable method of sustaining the development and yield of drought-stressed rice plants. The current study focused on the alleviation of drought in the early stages of rice variety CO 51 using PGPR isolated from the rhizosphere of xerophytes. The seeds were treated with bio inoculants and subjected to different moisture stress levels (10%, 20% and 30%) using PEG 6000. The seeds treated with bio inoculants exhibited higher germination percentage and growth traits such as shoot length root length and fresh weight, especially seeds treated with Bacillus velezensis VKSB5 (MT729963), and Bacillus altitudinis MLSB2 (MT729964) over uninoculated plants. This was found to be due to the increased proline accumulation and antioxidant activity in these seedlings, which plays a major role in drought alleviation by altering the osmotic potential and by its ROS scavenging mechanism. Hence this study provides evidence for the effective drought ameliorating ability of these cultures during the initial growth stages of rice. Further studies can contribute to the development of effective bio-inoculants for the mitigation of drought in rice.
Article Details
Article Details
Keywords
Bacillus, Drought, Moisture stress, Plant growth-promoting rhizobacteria, Rice
References
Azevedo, R.A.D., R.M. Alas, R.J. Smith & P.J. Lea. (1998). Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild‐type and a catalase‐deficient mutant of barley. Physiologia Plantarum, 104(2), 280-292. doi.org/10.1034/j.1399-3054.1998.1040217.x.
Bates, L.S., R.P. Waldren & I.D. Teare. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205-207. doi.org/10.1007/BF00018060.
Beauchamp, C. & I. Fridovich. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1), 276-287. doi.org/10.1016/0003-2697(71)90370-8.
Boretti, A. & L. Rosa. (2019). Reassessing the projections of the world water development report. NPJ Clean Water, 2(1), 1-6. doi.org/10.1038/s41545-019-0039-9.
Cai, J., Y. He, R. Xie & Y. Liu. (2020). A footprint-based water security assessment: An analysis of hunan province in china. Journal of Cleaner Production, 245, 118485. doi.org/10.1016/j.jclepro.2019.118485.
Fahad, S., A.A. Bajwa, U. Nazir, S.A. Anjum, A. Farooq, A. Zohaib, S. Sadia, W. Nasim, S. Adkins & S. Saud. (2017). Crop production under drought and heat stress: Plant responses and management options. Frontiers in Plant Science, 1147. doi.org/10.3389/fpls.2017.01147.
Farooq, M., A. Wahid, N. Kobayashi, D. Fujita & S.M.A. Basra, 2009. Plant drought stress: Effects, mechanisms and management. In: Sustainable agriculture. Springer: pp: 153-188. doi.org/10.1007/978-90-481-2666-8_12.
Grover, M., S.Z. Ali, V. Sandhya, A. Rasul & B. Venkateswarlu. (2011). Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World Journal of Microbiology and Biotechnology, 27(5), 1231-1240. doi.org/10.1007/s11274-010-0572-7.
Hammerschmidt, R., E.M. Nuckles & J. Kuć. (1982). Association of enhanced peroxidase activity with induced systemic resistance of cucumber to colletotrichum lagenarium. Physiological Plant Pathology, 20(1), 73-82. doi.org/1 0.1016/0048-4059(82)90025-X.
Hoekstra, A.Y., A.K. Chapagain, M.M. Aldaya & M.M. Mekonnen, 2011. The water footprint assessment manual: Setting the global standard. Routledge. doi.org/10.43 24/9781849775526.
Jabborova, D., A. Kannepalli, K. Davranov, A. Narimanov, Y. Enakiev, A. Syed, A.M. Elgorban, A.H. Bahkali, S. Wirth & R.Z. Sayyed. (2021). Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions. Scientific Reports, 11(1), 1-9. doi.org/10.1038/s41598-021-01337-9.
Karvembu, P., V. Gomathi, R. Anandham & J.K. Mary. (2021). Isolation, screening and identification of moisture stress tolerant rhizobacteria from xerophyte prosopis juliflora (sw). J. Pharmacogn. Phytochem, 9, 605-609. doi.org/10.22271/phyto.2020.v9.i6i.12981.
Kaya, M.D., G. Okçu, M. Atak, Y. Cıkılı & Ö. Kolsarıcı. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (helianthus annuus l.). European Journal of Agronomy, 24(4), 291-295. doi.org/10.1016/j.eja.2005.08.001.
Khan, N. & A. Bano. (2019). Exopolysaccharide producing rhizobacteria and their impact on growth and drought tolerance of wheat grown under rainfed conditions. PLoS One, 14(9), e0222302. doi.org/10.1371/journal.po ne.0222302.
Kumar, S., A.S. Beena, M. Awana & A. Singh. (2017). Physiological, biochemical, epigenetic and molecular analyses of wheat (Triticum aestivum) genotypes with contrasting salt tolerance. Frontiers in Plant Science, 8, 1151. doi.org/10.3389/fpls.2017.01151.
Li, Y., H. Shi, H. Zhang & S. Chen. (2019). Amelioration of drought effects in wheat and cucumber by the combined application of super absorbent polymer and potential biofertilizer. PeerJ, 7, e6073. doi.org/10.7717/peerj.6073.
Moaveni, P. (2011). Effect of water deficit stress on some physiological traits of wheat (triticum aestivum). Agric. Sci. Res. J, 1(1), 64-68.
Mumtaz, M.Z., M. Saqib, G. Abbas, J. Akhtar & Z. Ul-Qamar. (2020). Drought stress impairs grain yield and quality of rice genotypes by impaired photosynthetic attributes and k nutrition. 收藏, 1.
Nakano, Y. & K. Asada. (1987). Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiology, 28(1), 131-140. doi.org/10.1093/oxfordjournals.pcp.a077268.
Petrov, V., J. Hille, B. Mueller-Roeber & T.S. Gechev. (2015). Ros-mediated abiotic stress-induced programmed cell death in plants. Frontiers in Plant Science, 6, 69. doi.org/10.3389/fpls.2015.00069.
Rashid, U., H. Yasmin, M.N. Hassan, R. Naz, A. Nosheen, M. Sajjad, N. Ilyas, R. Keyani, Z. Jabeen & S. Mumtaz. (2021). Drought-tolerant bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions. Plant Cell Reports, 1-21. doi.org/10.1007/s00299-020-02640-x.
Sandhya, V., A. Sk Z, M. Grover, G. Reddy & B. Venkateswarlu. (2009). Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing pseudomonas putida strain gap-p45. Biology and Fertility of Soils, 46(1), 17-26. doi.org/10.1007/s00374-009-0 401-z.
Vurukonda, S.S.K.P., S. Vardharajula, M. Shrivastava & A. SkZ. (2016). Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184, 13-24. doi.org/10.1016/j.micr es.2015.12.003.
Xia, Y., M.A. Farooq, M.T. Javed, M.A. Kamran, T. Mukhtar, J. Ali, T. Tabassum, S. ur Rehman, M.F.H. Munis & T. Sultan. (2020). Multi-stress tolerant pgpr bacillus xiamenensis pm14 activating sugarcane (Saccharum officinarum l.) red rot disease resistance. Plant Physiology and Biochemistry, 151, 640-649. doi.org/10.1016/j.plaphy.2020.04.016.
Zhang, X., Y. Mi, H. Mao, S. Liu, L. Chen & F. Qin. (2020). Genetic variation in zmtip1 contributes to root hair elongation and drought tolerance in maize. Plant Biotechnology Journal, 18(5), 1271-1283. doi.org/10.1111/pbi.13290.
Bates, L.S., R.P. Waldren & I.D. Teare. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205-207. doi.org/10.1007/BF00018060.
Beauchamp, C. & I. Fridovich. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1), 276-287. doi.org/10.1016/0003-2697(71)90370-8.
Boretti, A. & L. Rosa. (2019). Reassessing the projections of the world water development report. NPJ Clean Water, 2(1), 1-6. doi.org/10.1038/s41545-019-0039-9.
Cai, J., Y. He, R. Xie & Y. Liu. (2020). A footprint-based water security assessment: An analysis of hunan province in china. Journal of Cleaner Production, 245, 118485. doi.org/10.1016/j.jclepro.2019.118485.
Fahad, S., A.A. Bajwa, U. Nazir, S.A. Anjum, A. Farooq, A. Zohaib, S. Sadia, W. Nasim, S. Adkins & S. Saud. (2017). Crop production under drought and heat stress: Plant responses and management options. Frontiers in Plant Science, 1147. doi.org/10.3389/fpls.2017.01147.
Farooq, M., A. Wahid, N. Kobayashi, D. Fujita & S.M.A. Basra, 2009. Plant drought stress: Effects, mechanisms and management. In: Sustainable agriculture. Springer: pp: 153-188. doi.org/10.1007/978-90-481-2666-8_12.
Grover, M., S.Z. Ali, V. Sandhya, A. Rasul & B. Venkateswarlu. (2011). Role of microorganisms in adaptation of agriculture crops to abiotic stresses. World Journal of Microbiology and Biotechnology, 27(5), 1231-1240. doi.org/10.1007/s11274-010-0572-7.
Hammerschmidt, R., E.M. Nuckles & J. Kuć. (1982). Association of enhanced peroxidase activity with induced systemic resistance of cucumber to colletotrichum lagenarium. Physiological Plant Pathology, 20(1), 73-82. doi.org/1 0.1016/0048-4059(82)90025-X.
Hoekstra, A.Y., A.K. Chapagain, M.M. Aldaya & M.M. Mekonnen, 2011. The water footprint assessment manual: Setting the global standard. Routledge. doi.org/10.43 24/9781849775526.
Jabborova, D., A. Kannepalli, K. Davranov, A. Narimanov, Y. Enakiev, A. Syed, A.M. Elgorban, A.H. Bahkali, S. Wirth & R.Z. Sayyed. (2021). Co-inoculation of rhizobacteria promotes growth, yield, and nutrient contents in soybean and improves soil enzymes and nutrients under drought conditions. Scientific Reports, 11(1), 1-9. doi.org/10.1038/s41598-021-01337-9.
Karvembu, P., V. Gomathi, R. Anandham & J.K. Mary. (2021). Isolation, screening and identification of moisture stress tolerant rhizobacteria from xerophyte prosopis juliflora (sw). J. Pharmacogn. Phytochem, 9, 605-609. doi.org/10.22271/phyto.2020.v9.i6i.12981.
Kaya, M.D., G. Okçu, M. Atak, Y. Cıkılı & Ö. Kolsarıcı. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (helianthus annuus l.). European Journal of Agronomy, 24(4), 291-295. doi.org/10.1016/j.eja.2005.08.001.
Khan, N. & A. Bano. (2019). Exopolysaccharide producing rhizobacteria and their impact on growth and drought tolerance of wheat grown under rainfed conditions. PLoS One, 14(9), e0222302. doi.org/10.1371/journal.po ne.0222302.
Kumar, S., A.S. Beena, M. Awana & A. Singh. (2017). Physiological, biochemical, epigenetic and molecular analyses of wheat (Triticum aestivum) genotypes with contrasting salt tolerance. Frontiers in Plant Science, 8, 1151. doi.org/10.3389/fpls.2017.01151.
Li, Y., H. Shi, H. Zhang & S. Chen. (2019). Amelioration of drought effects in wheat and cucumber by the combined application of super absorbent polymer and potential biofertilizer. PeerJ, 7, e6073. doi.org/10.7717/peerj.6073.
Moaveni, P. (2011). Effect of water deficit stress on some physiological traits of wheat (triticum aestivum). Agric. Sci. Res. J, 1(1), 64-68.
Mumtaz, M.Z., M. Saqib, G. Abbas, J. Akhtar & Z. Ul-Qamar. (2020). Drought stress impairs grain yield and quality of rice genotypes by impaired photosynthetic attributes and k nutrition. 收藏, 1.
Nakano, Y. & K. Asada. (1987). Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiology, 28(1), 131-140. doi.org/10.1093/oxfordjournals.pcp.a077268.
Petrov, V., J. Hille, B. Mueller-Roeber & T.S. Gechev. (2015). Ros-mediated abiotic stress-induced programmed cell death in plants. Frontiers in Plant Science, 6, 69. doi.org/10.3389/fpls.2015.00069.
Rashid, U., H. Yasmin, M.N. Hassan, R. Naz, A. Nosheen, M. Sajjad, N. Ilyas, R. Keyani, Z. Jabeen & S. Mumtaz. (2021). Drought-tolerant bacillus megaterium isolated from semi-arid conditions induces systemic tolerance of wheat under drought conditions. Plant Cell Reports, 1-21. doi.org/10.1007/s00299-020-02640-x.
Sandhya, V., A. Sk Z, M. Grover, G. Reddy & B. Venkateswarlu. (2009). Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing pseudomonas putida strain gap-p45. Biology and Fertility of Soils, 46(1), 17-26. doi.org/10.1007/s00374-009-0 401-z.
Vurukonda, S.S.K.P., S. Vardharajula, M. Shrivastava & A. SkZ. (2016). Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184, 13-24. doi.org/10.1016/j.micr es.2015.12.003.
Xia, Y., M.A. Farooq, M.T. Javed, M.A. Kamran, T. Mukhtar, J. Ali, T. Tabassum, S. ur Rehman, M.F.H. Munis & T. Sultan. (2020). Multi-stress tolerant pgpr bacillus xiamenensis pm14 activating sugarcane (Saccharum officinarum l.) red rot disease resistance. Plant Physiology and Biochemistry, 151, 640-649. doi.org/10.1016/j.plaphy.2020.04.016.
Zhang, X., Y. Mi, H. Mao, S. Liu, L. Chen & F. Qin. (2020). Genetic variation in zmtip1 contributes to root hair elongation and drought tolerance in maize. Plant Biotechnology Journal, 18(5), 1271-1283. doi.org/10.1111/pbi.13290.
Issue
Section
Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
How to Cite
Plant growth-promoting rhizobacteria mediated moisture stress alleviation in the early stages of Rice (Oryza sativa L.) variety CO 51. (2022). Journal of Applied and Natural Science, 14(4), 1124-1129. https://doi.org/10.31018/jans.v14i4.3776
