Efficiency of gamma irradiation and ethyl methane sulphonate in inducing variations in Jasminum auriculatum Vahl.
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Abstract
Vegetatively propagated crops like Jasminum auriculatum have a constraint in genetic variation due to a narrow genetic base that restricts the insights in any crop breeding programme. Mutation breeding is a potential tool that directs a way to create desirable variation effectively in vegetatively propagated crops. The optimum dose of mutagen is the one which produces the maximum frequency of mutation in turn the variation, with minimum killing. The aim of the present paper unveils the Lethal Dose (LD50) and the growth reduction dose (GR50) for both Gamma irradiation (GI) and Ethyl methane sulphonate (EMS) for the ecotype ‘Muthu Mullai’ of Jasminum auriculatum. These parameters of GR and EMS treated cuttings were analysed by considering the GR50 values of the mortality rate (57%, 48%), survival percentage (12.479Gy, 13.268mM), shoot length (18.59Gy, 18.28mM), root length (20.39Gy, 18.17mM), number of leaves (22.29Gy, 17.47mM), number of sprouts (22.97Gy, 16.17mM), vigour index (10.43Gy, 11.05mM), leaf length (21.61Gy, 19.90mM) and leaf width (19.2Gy, 16.17mM). The LD50 value was 12.479 Gy for GI and 13.268 mM for EMS treatment. The GR50 for different growth parameters ranged from 14.93 to 22.9 Gy for GI and 1.05 to 19.9 mM for EMS treatment. The mutagenic efficiency and effectiveness were 214.96 and 89.36 for GI and 48.66 and 33.77 for EMS treatment, respectively. These doses can be used for generating considerable variation, which can be put to use in future crop improvement programmes for Jasmine.
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Effectiveness, GR50, LD50, Mutation, Mutagenic efficiency
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