Pollen storage, viability and effect of growth hormones on in vitro pollen germination in two medicinal plants (Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L.) of the tropical moist forest of North-east India
Article Main
Puyam Devanda Singh
Kewat Sanjay Kumar
https://orcid.org/0000-0003-2951-1835
Vinod Prasad Khanduri
https://orcid.org/0000-0003-2951-1835
Vinod Prasad Khanduri
Abstract
Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L. are well known for their medicinal uses in treating various human ailments traditionally. Pollen biology study was done in both plant species to decipher pollen viability, in vitro pollen germination, and pollen storage in varied temperature conditions. Pollen viability tests was done by 2, 3, 5-triphenyl tetrazolium chloride (TTC test) for which pollen grains were collected at the anthesis stage that ascertained high viability, ranged between 71.97%±4.30 % in C. colebrookianum and 81.63%±3.23 in C. infortunatum. In vitro pollen germination was conducted under different hormones (IBA, IAA, GA3, and Kinetin) with concentrations of 100 mg L-1, 200 mg L-1 and 300 mg L-1. Highest germination percentage of 52.10±5.30% and 61.91±1.76% in GA3 (200 mg L-1) was the most suitable growth hormone concentration for inducing in vitro pollen germination in both Clerodendrum species. Statistically, the response of all the concentrations of treatments, sucrose, and hormones, with their time on in vitro pollen germination of C. colebrookianum and C. infortunatum was found significantly different (p<0.05). In contrast, non-significant differences were recorded for in vitro pollen germination between the medicinal plant species. Pollen storage under temperature gradient conditions exhibited a similar trend in the viability for both C. colebrookianum, and C. infortunatum, i.e., the pollen remained viable up to 28 days at -20°C and 6°C, respectively. This study will be helpful in future breeding, hybridization, and conservation efforts for both the Clerodendrum species.
Article Details
Article Details
Keywords
Clerodendrum colebrookianum, Hormones, Medicinal uses, Pollen germination
References
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Kormutak, A., Bolecek, P., Galgóci, M. & Gomory, D. (2021). Longevity and germination of Juniperus communis L. pollen after storage. Scientific Reports, 11(1), 1-8. doi.org/10.1038/s41598-021-90942-9
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Kshetri, P., Tamreihao, K., Roy, S. S., Singh, T. S., Sharma, S. K. & Ansari, M. A. (2022). Ethnomedicinal plants of north eastern Himalayan region of India to combat hypertension. In Medicinal Plants (pp. 461-483). 1st Ed. New York, Apple Academic Press. doi.org/10.1201/97810 03277408
Li, Y., Tang, G. & Luo, F. (2015). Studies on pollen viability and cross-compatibility of four species of Hibiscus. Journal of Nanjing Forestry University (Natural Sciences Edition), 39(2), 183-186. doi.org/10.3969/j.issn.1000-2006.2015.02.032
Lin, Y., Wang, Y., Iqbal, A., Shi, P., Li, J., Yang, Y. & Lei, X. (2017). Optimization of culture medium and temperature for the in vitro germination of oil palm pollen. Scientia Horticulturae, 220, 134-138. doi.org/10.1016/j.scienta.201 7.03.040
Lokesh, D. & Amitsankar, D. (2012). Evaluation of mechanism for antihypertensive action of Clerodendrum colebrookianum Walp., used by folklore healers in north-east India. Journal of Ethnopharmacology, 143(1), 207-212. doi.org/10.1016/j.jep.2012.06.025
Maita, S. & Sotomayor, C. (2015). The effect of three plant bioregulators on pollen germination, pollen tube growth and fruit set in almond [Prunus dulcis (Mill.) DA Webb] cvs. Non Pareil and Carmel. Electronic Journal of Biotechnology, 18(5), 381-386. doi.org/10.1016/j.ejbt.2015.07.004
Manonmani, R. & Mekala, R. (2016). Pollen morphology, viability, germination and tube growth of some plant species. Ecology, 3(4), 5-6. doi.10.20959/wjpr20167-6437
Mao, A. A., Wetten, A., Fay, M. & Caligari, P. D. S. (1995). In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant. Plant Cell Reports, 14(8), 493-496. doi.org/10.1007/BF00232781
Marchioretto, L. D. R., De Rossi, A., do Amaral, L. O. & de Souza Ribeiro, A. M. A. (2019). Efficacy and mode of action of blossom thinners on ‘Fuji More’apple trees. Scientia Horticulturae, 246, 634-642. doi.org/10.101 6/j.scienta.2018.11.039
Marques, J. E. N. (2018). Assays of In vitro germination of kiwi pollen (Doctoral dissertation, Universidade de Coimbra). hdl.handle.net/10316/86144
Mesnoua, M., Roumani, M. & Salem, A. (2018). The effect of pollen storage temperatures on pollen viability, fruit set and fruit quality of six date palm cultivars. Scientia Horticulturae, 236, 279-283. doi.org/10.1016/j.scienta.2018.0 3.053
Murtem, G. & Chaudhry, P. (2016). An ethnobotanical study of medicinal plants used by the tribes in upper Subansiri district of Arunachal Pradesh, India. American journal of ethnomedicine, 3(3), 35-49. www.ajethno.com
Naik, A., Akhtar, S., Chattopadhyay, A., Thapa, U. & Hazra, P. (2016). In vitro teasle gourd pollen germination and pollen tube development as affected by sucrose, boric acid, and inorganic salts. International Journal of Vegetable Science, 22(2), 209-216. doi.org/10.1080/19315 260.2015.1008665
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Pollen storage, viability and effect of growth hormones on in vitro pollen germination in two medicinal plants (Clerodendrum colebrookianum Walp. and Clerodendrum infortunatum L.) of the tropical moist forest of North-east India. (2022). Journal of Applied and Natural Science, 14(3), 999-1008. https://doi.org/10.31018/jans.v14i3.3737
