Effect of elicitors on physiomorphological and biochemical parameters of Indian mustard (Brassica juncea) and rapeseed (B. napus)
Article Main
Abstract
The present investigation was aimed to assess the effect of different concentrations of elicitors viz., salicylic acid (SA) and benzothiadiazole (BTH) on physiomorphological and biochemical parameters of Brassica juncea and B. napus cultivars. The field experiment was conducted during 2012-13 at Punjab Agricultural University, Ludhiana, India. Four different treatments of elicitors and a fungicide treatment were given to 10 week old plants up to four consecutive weeks. Content of total soluble protein, free amino acids, total sugars and reducing sugars of
leaves of both B. juncea and B. napus were evaluated after each spray. The content of total soluble protein increased, whereas the contents of free amino acids, total sugars and reducing sugars decreased in all the treatments after each spray. Among all the treatments, the combinations of elicitors, i.e., BTH (3 ppm) + SA (33 ppm) and BTH (7 ppm) + SA (17 ppm) exhibited maximum contents of total soluble protein, free amino acids, total sugars and reducing sugars than control in both the cultivars. These treatments were more pronounced in increasing plant height, internodal distance and number of pods per plant in both the cultivars. Our results suggest that the combinations of elicitors act synergistically to promote growth and metabolic activities in B. juncea and B. napus cultivars leading to the induction and regulation of disease resistance.
Article Details
Article Details
Benzothiadiazole, Brassica, Free amino acids, Salicylic acid, Total soluble protein, Total sugars
Amin, A.A., Rashad, EL-Sh.M. and EL-Abagy, H.M.H. (2007). Physiological effect of indole-3-butyric acid and salicylic acid on growth, yield and chemical constituents of onion plants. Journal of Applied Science Research, 3: 1554-1563.
Couee, I., Sulmon, C., Gouesbet, G. and Amrani, A.E.l. (2006). Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. Journal of Experimental Botany, 57: 449-459.
Daayf, F., Ongena, M., Boulanger, R., Hadrami, I.El and Belanger, R.R. (2000). Induction of phenolic compounds in two cultivars of cucumber by treatment of healthy and powdery mildew-infected plants with extracts of Reynoutria sachalinensis. Journal of Chemical Ecology, 26: 1579-1593.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356.
El-Khallal, S.M. (2007). Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (jasmonic acid and salicylic acid): Changes in growth, some metabolic activities and endogenous hormones related to defense mechanism. Australian Journal of Basic and Applied Sciences, 1:691-705.
Farouk, S. and Osman, M.A. (2011). The effect of plant defense elicitors on common bean (Phaseolus vulgaris L.) growth and yield in absence or presence of spider mite (Tetranychus urticae Koch) infestation. Journal of Stress Physiology & Biochemistry, 7: 5-22.
Farouk, S. and Osman, M.A. (2012). Alleviation of oxidative stress induced by spider mite invasion through application of elicitors in bean plants. Egyptian Journal of Biology, 14: 1-13.
Farouk, S., Ghoneem, K.M. and Ali, A.A. (2008). Induction and expression of systemic resistance to downy mildew disease in cucumber by elicitors. Egyptian Journal of Phytopathology, 36: 95-111.
Jaypal, R. and Mahadevan, A. (1968). Biochemical changes in banana leaves in response to leaf spot pathogenesis. Indian Phytopathology, 21: 43-48.
Jayraman, J. (1981). Protein fractionation in gram flour. Laboratory Manual in Biochemistry. Wiley Eastern Ltd., New Delhi, pp 79-80.
Lawton, K.A., Friedrich, L., Hunt, M., Weyman, K., Delaney, T.P., Kessmann, H., Staub, T. and Ryals, J. (1996). Benzothiadiazole includes disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant Journal, 10: 71-82.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. The J. of Biological Chemistry, 193:265-275.
Mandavia, M.K., Raval, L., Mandavia, C. and Karkar, C. (2012). Effect of brassinolide and salicylic acid on biochemical parameters and yield of groundnut. Indian Journal of Agricultural Biochemistry, 25: 20-24.
Meena, P.D., Awasthi, R.P., Chattopadhyay, C., Kolte, S.J. and Kumar, A. (2010). Alternaria blight: a chronic disease in rapeseed-mustard. Journal of Oilseed Brassica, 1: 1-11.
Miller, G.L. (1972). Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry, 31: 426-428.
Misra, R., Sharma, S., Mishra, K., Kumar, A. and Sriram S. (2008). Biochemical alterations induced in Taro in response to Phytophthora colocasiae infection. Advances in Natural and Applied Sciences, 2: 112-121.
Onifade, A.K. and Agboola, Y.A.J. (2003). Effect of fungal infection on proximate nutrient composition of coconut (Cocos nucifera Linn) fruit. Journal of Food, Agriculture and Environment, 1: 141-142.
Raskin, I. (1992). Role of salicylic acid in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 43: 439-463.
Shakirova, F.M., Sakhabutdinova, A.R., Bezrukova, M.V., Fatkhutdinova, R.A. and Fatkhutdinova, D.R. (2003). Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science, 164: 317-322.
Singh, Y., Rao, D.V. and Batra, A. (2011). Biochemical changes in Brassica juncea (L.) Czern. and Coss. infected with Albugo candida Kuntz. (Pers.). International Journal of Pharmaceutical Sciences Review and Research, 7: 74-78.
Vernooij, B., Friedrich, L., Ahl Goy, P., Staub, T., Kessmann, H. and Ryals, J. (1995). 2,6-Dichloroisonicotinic acid induced resistance to pathogens without the accumulation of salicylic acid. Molecular Plant Microbe Interaction, 8: 2228-2234.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
