Assessment of rice (Co 51) seed ageing through volatile organic compound analysis using Headspace-Solid Phase Micro Extraction/ Gas Chromatography-Mass Spectrometry (HS-SPME/GCMS)
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Abstract
Seed ageing is an inevitable process that reduces seed quality during storage. When seeds deteriorate as a result of the lipid peroxidation process, it leads to produce toxic volatile organic compounds. These volatiles served as an indicator for the viability of stored seeds. With this background, the study was conducted to profile the volatile organic compounds emitted from rice seeds during storage. Volatile profiling of stored rice var. Co 51 seeds was done through Headspace-Solid phase microextraction/ Gas chromatography-mass spectrometry (HS-SPME/GCMS). The study clearly demonstrated that the significant decrease in physiological and biochemical quality attributes was noted due to an increase in the strength of volatiles released during ageing. When the release of total volatile strength reached more than 40%, a significant reduction in physiological attributes such as germination, root and shoot length, dry matter production and vigour index were observed. With respect to biochemical properties, a significant increase in electrical conductivity of seed leachate, lipid peroxidation and lipoxygenase activity, and decrease in dehydrogenase, catalase and peroxidase activities were observed. However, the highest reduction in all these properties were recorded when the total volatile strength reached to 54.90%. Finally, the study concluded that, among all the volatiles, 1-hexanol, 1-butanol, ethanol, hexanal, acetic acid, hexanoic acid and methyl ester were the most closely associated volatiles with seed deterioration. It indicates that these components could be considered the signature components for assessing the seed quality in rice during storage.
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Rice, Seed deterioration, Seed quality, volatile organic compounds.
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