Assessment of genetic diversity in pigeon pea (Cajanus cajan) using micro satellite markers
##plugins.themes.bootstrap3.article.main##
https://orcid.org/0000-0002-9566-5368
Ashwani Kumar
Neha Sharma
Abstract
An effective way to use germplasm for genetic improvement is to be aware of the genetic variation among crop genotypes. The objective of the present study was to assess the genetic diversity and population structure of 30 genotypes of pigeonpea from populations that were collected from various sources. In order to show a new structure within the pigeonpea genetic pool and to give crucial information for pigeonpea breeding operations, the predetermined study's goal was to define pigeonpea genotypes using a microsatellite marker technique. The genomic DNA of 30 pigeon pea genotypes were amplified with 20 SSR primers that produced 46 amplified bands, out of which 30 band were polymorphic (65.21%) and 16 bands were monomorphic (34.82%). Primer CcM 2977 generated a maximum number of amplified bands, of which 2 bands were polymorphic. Among 20 primers, only 8 primers showed the highest polymorphism (100%) and 5 primers were monomorphic in nature. Average of 2.30 bands per primer was amplified. The dendrogram constructed from the pooled data revealed six distinct clusters of which five were solitary. Cluster analysis of pigeon pea genotypes was based on Unweighted Pair Group Method with the Arithmetic average (UPGMA) method and the 30 pigeon pea genotypes were classified into six main groups. The present study indicated that the performance of SSR markers for the evaluation of genetic diversity could be beneficial for pigeon pea breeding. They could additionally be useful in genomic mapping research, developing pigeon pea cultivars with various genetics and reaping advanced crop productivity.
##plugins.themes.bootstrap3.article.details##
##plugins.themes.bootstrap3.article.details##
Genetic diversity, Pigeon pea, Microsatellite marker, UPGMA method
Adjebeng-Danquah, J., Manu-Aduening, J., Asante, I. K., Agyare, R. Y., Gracen, V. & Offei, S. K. (2020). Genetic diversity and population structure analysis of Ghanaian and exotic cassava accessions using simple sequence repeat (SSR) markers. Heliyon, 6(1), e03154.
Addae-Frimpomaah, F., Denwar, N. N., Adazebra, G. A., Adjebeng-Danquah, J., Kanton, R. A. L., Afful, N. T. & Duut, M. (2021). Assessment of genetic diversity among 32 pigeon pea genotypes using morphological traits and simple sequence repeat markers in Northern Ghana. CSIR Space Ghana, URI: http://hdl.handle.net/1234 56789/1996.
Adjebeng-Danquah J, Manu-Aduening J, Asante IK, Agyare RY, Gracen V. & Offei SK. (2020). Genetic diversity and population structure analysis of Ghanaian and exotic cassava accessions using simple sequence repeat (SSR) markers. Heliyon. 6(1):e03154. doi: 10.1016/j.heliyon.2019.e03154. PMID: 32042951; PMCID: PMC7002781.
Asare EK, Jaiswal S, Maley J, Båga M, Sammynaiken R, Rossnagel BG. & Chibbar RN. (2011). Barley grain constituents, starch composition, and structure affect starch in vitro enzymatic hydrolysis. J Agric Food Chem. 59(9):4743-54. doi: 10.1021/jf200054e. Epub 2011 Apr 19. PMID: 21462932.
Carvalho LJCB, & Schaal BA. (2001). Assessing genetic diversity in the cassava (Manihot esculenta Crantz) germplasm collection in Brazil using PCR-based markers. Euphytica 120: 133-142
Elameen, A., Larsen, A., Klemsdal, S. S., Fjellheim, S., Sundheim, L., Msolla, S. & Rognli, O. A. (2011). Phenotypic diversity of plant morphological and root descriptor traits within a sweet potato, Ipomoea batatas (L.) Lam., germplasm collection from Tanzania. Genetic Resources and Crop Evolution, 58(3), 397-407.
Jaccard, P. (1908). Nouvelles reserches sur la distribution florale. Bulletin Societe Vaudoise Des Sciences Naturelles. 44: 223-270.
Kinhoegbe, G., Djedatin, G., Saxena, R.K., Chitikineni, A., Bajaj, P., Molla J., Agbangla, C., Dansi, A. and Varshney, R.K. (2022) Genetic diversity and population structure of pigeonpea (Cajanus cajan [L.] Millspaugh) landraces grown in Benin revealed by Genotyping-By-Sequencing. PLoS ONE 17(7): e0271565.
Kimaro, D., Melis, R., Sibiya, J., Shimelis, H. and Shayanowako, A. (2020) Analysis of Genetic Diversity and Population Structure of Pigeonpea [Cajanus cajan (L.) Millsp] Accessions Using SSR Markers. Plants 9(12),1-14 1643. Kumar, A., Sengar, R.S., Rao, V.P., Shukla, G., Dixit, R. & Singh,R. (2017). Assessment of Genetic diversity in bread wheat (Triticum aestivum L.) using RAPD markers. J. Appl. & Nat. Sci. 9 (3): 1751 -1755.
Kumar, A., Goswami, A., Kumar, R., Chaudhary, B., Sagar, A. and Sengar, R.S. (2018). Assessment of Genetic Diversity in Indian Wheat cultivar’s (Triticum aestivum L.) By Using SSR marker Prog. Agric. 18 (2): 240-245.
Kumar, A., Singh, R., Singh, R. & Sengar, S. R. (2015). Molecular Approach for Detection of Plant pathogen. An International Journal of Biological Sciences Biotech today. 5(2):14 -19.
Kumar, S., Deepali, Mishra D. C., Bansal R., Kumari J. & Kumar S. (2016). Molecular characterization of Indian wheat germplasm line for stay green & other heat tolerance genes using linked SSR markes. An International Jouranl of Biological Sciences Biotech today. 6(1):90 -94. Mafakheri, K., Bihamta, M. R. & Abbasi, A. R. (2017). Assessment of genetic diversity in cowpea (Vigna unguiculata L.) germplasm using morphological and molecular characterisation. Cogent Food & Agriculture, 3(1), 1327 092.
Nei, M. & Li, W. H. (1979). Mathematical models for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76:5269-5273.
Ojuederie OB, Balogun MO, Fawole I, Igwe DO. & Olowolafe MO. (2014). Assessment of the genetic diversity of African yam bean (Sphenostylis stenocarpa Hochst ex. A Rich Hams) accessions using amplified fragment length polymorphism (AFLP) markers. Afr. J. Biotechnol. 13(18): 1850-1858
Pandit, S. Rathod, Sharma, A., Patil, D.H. & Dodamani, B.M. (2015). Performance of pigeon pea under different sources of nutrientsin rainfed conditions of Karnataka, Journal of Food Legumes. 28(2): 43-45.
Palombi MA. & Damiano C. (2002). Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Rep. (20).1061-1066.
Russell JR, Fuller JD, Macaulay M, Hatz BG, Jahoor A, Powell W & Waugh R. (1997). Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor. Appl. Genet. 95:714-722.
Sarkar. S, S. Panda. S, Yadav K.K. & Kandasamy P. (2018). Pigeon pea (Cajanus cajan) an important food legume in Indian scenario – A review. Legume Research. LR-4021: 1-10.
Sathyanarayana, N., Pittala, R. K., Tripathi, P. K., Chopra, R., Singh, H. R., Belamkar, V. & Egan, A. N. (2017). Transcriptomic resources for the medicinal legume Mucuna pruriens: de novo transcriptome assembly, annotation, identification and validation of EST-SSR markers. BMC genomics, 18(1), 1-18.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
